Amino acid dipepetide frequency for Deefgea sp. D17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.894AlaAla: 13.894 ± 0.205
1.147AlaCys: 1.147 ± 0.037
5.555AlaAsp: 5.555 ± 0.076
6.533AlaGlu: 6.533 ± 0.098
3.602AlaPhe: 3.602 ± 0.06
7.892AlaGly: 7.892 ± 0.11
2.283AlaHis: 2.283 ± 0.052
6.589AlaIle: 6.589 ± 0.099
5.227AlaLys: 5.227 ± 0.097
12.651AlaLeu: 12.651 ± 0.134
3.262AlaMet: 3.262 ± 0.066
3.941AlaAsn: 3.941 ± 0.07
4.079AlaPro: 4.079 ± 0.074
6.012AlaGln: 6.012 ± 0.111
5.403AlaArg: 5.403 ± 0.086
6.412AlaSer: 6.412 ± 0.098
5.259AlaThr: 5.259 ± 0.089
7.071AlaVal: 7.071 ± 0.101
1.57AlaTrp: 1.57 ± 0.045
2.513AlaTyr: 2.513 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.973CysAla: 0.973 ± 0.03
0.16CysCys: 0.16 ± 0.013
0.569CysAsp: 0.569 ± 0.024
0.508CysGlu: 0.508 ± 0.023
0.359CysPhe: 0.359 ± 0.019
0.934CysGly: 0.934 ± 0.03
0.309CysHis: 0.309 ± 0.019
0.573CysIle: 0.573 ± 0.025
0.33CysLys: 0.33 ± 0.018
1.052CysLeu: 1.052 ± 0.033
0.211CysMet: 0.211 ± 0.015
0.332CysAsn: 0.332 ± 0.019
0.463CysPro: 0.463 ± 0.026
0.464CysGln: 0.464 ± 0.019
0.515CysArg: 0.515 ± 0.023
0.679CysSer: 0.679 ± 0.029
0.461CysThr: 0.461 ± 0.021
0.662CysVal: 0.662 ± 0.03
0.148CysTrp: 0.148 ± 0.012
0.281CysTyr: 0.281 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
5.729AspAla: 5.729 ± 0.082
0.588AspCys: 0.588 ± 0.025
2.543AspAsp: 2.543 ± 0.053
3.232AspGlu: 3.232 ± 0.072
2.514AspPhe: 2.514 ± 0.057
3.868AspGly: 3.868 ± 0.087
1.015AspHis: 1.015 ± 0.031
3.034AspIle: 3.034 ± 0.059
2.08AspLys: 2.08 ± 0.052
5.664AspLeu: 5.664 ± 0.082
1.18AspMet: 1.18 ± 0.035
1.536AspAsn: 1.536 ± 0.047
2.296AspPro: 2.296 ± 0.06
2.243AspGln: 2.243 ± 0.051
2.424AspArg: 2.424 ± 0.053
2.594AspSer: 2.594 ± 0.059
2.238AspThr: 2.238 ± 0.052
3.786AspVal: 3.786 ± 0.089
0.926AspTrp: 0.926 ± 0.032
1.638AspTyr: 1.638 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
5.49GluAla: 5.49 ± 0.088
0.444GluCys: 0.444 ± 0.021
2.016GluAsp: 2.016 ± 0.06
2.372GluGlu: 2.372 ± 0.063
2.004GluPhe: 2.004 ± 0.052
2.935GluGly: 2.935 ± 0.057
1.385GluHis: 1.385 ± 0.039
3.838GluIle: 3.838 ± 0.066
2.737GluLys: 2.737 ± 0.062
6.494GluLeu: 6.494 ± 0.093
1.608GluMet: 1.608 ± 0.039
1.924GluAsn: 1.924 ± 0.045
1.849GluPro: 1.849 ± 0.048
3.386GluGln: 3.386 ± 0.071
3.44GluArg: 3.44 ± 0.073
2.924GluSer: 2.924 ± 0.051
2.74GluThr: 2.74 ± 0.064
3.967GluVal: 3.967 ± 0.075
0.726GluTrp: 0.726 ± 0.027
1.42GluTyr: 1.42 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
4.374PheAla: 4.374 ± 0.073
0.507PheCys: 0.507 ± 0.024
2.414PheAsp: 2.414 ± 0.049
2.145PheGlu: 2.145 ± 0.05
1.686PhePhe: 1.686 ± 0.052
3.092PheGly: 3.092 ± 0.066
0.75PheHis: 0.75 ± 0.028
2.299PheIle: 2.299 ± 0.054
1.699PheLys: 1.699 ± 0.047
3.441PheLeu: 3.441 ± 0.08
0.944PheMet: 0.944 ± 0.035
1.704PheAsn: 1.704 ± 0.047
1.375PhePro: 1.375 ± 0.035
1.13PheGln: 1.13 ± 0.034
1.616PheArg: 1.616 ± 0.045
2.766PheSer: 2.766 ± 0.065
2.072PheThr: 2.072 ± 0.068
2.62PheVal: 2.62 ± 0.053
0.59PheTrp: 0.59 ± 0.026
1.179PheTyr: 1.179 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
6.317GlyAla: 6.317 ± 0.095
0.809GlyCys: 0.809 ± 0.031
3.511GlyAsp: 3.511 ± 0.058
4.126GlyGlu: 4.126 ± 0.077
3.265GlyPhe: 3.265 ± 0.064
5.256GlyGly: 5.256 ± 0.103
1.562GlyHis: 1.562 ± 0.042
4.617GlyIle: 4.617 ± 0.082
3.672GlyLys: 3.672 ± 0.069
7.618GlyLeu: 7.618 ± 0.1
2.123GlyMet: 2.123 ± 0.051
2.381GlyAsn: 2.381 ± 0.057
1.874GlyPro: 1.874 ± 0.048
3.464GlyGln: 3.464 ± 0.07
3.569GlyArg: 3.569 ± 0.066
4.003GlySer: 4.003 ± 0.074
3.276GlyThr: 3.276 ± 0.097
5.653GlyVal: 5.653 ± 0.082
1.015GlyTrp: 1.015 ± 0.039
2.083GlyTyr: 2.083 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.23HisAla: 2.23 ± 0.055
0.313HisCys: 0.313 ± 0.018
1.122HisAsp: 1.122 ± 0.033
1.118HisGlu: 1.118 ± 0.042
1.083HisPhe: 1.083 ± 0.032
1.726HisGly: 1.726 ± 0.045
0.664HisHis: 0.664 ± 0.028
1.268HisIle: 1.268 ± 0.039
0.727HisLys: 0.727 ± 0.031
2.475HisLeu: 2.475 ± 0.052
0.405HisMet: 0.405 ± 0.021
0.755HisAsn: 0.755 ± 0.026
1.289HisPro: 1.289 ± 0.038
1.086HisGln: 1.086 ± 0.035
1.153HisArg: 1.153 ± 0.036
1.306HisSer: 1.306 ± 0.04
1.047HisThr: 1.047 ± 0.036
1.239HisVal: 1.239 ± 0.036
0.414HisTrp: 0.414 ± 0.022
0.702HisTyr: 0.702 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
7.663IleAla: 7.663 ± 0.116
0.64IleCys: 0.64 ± 0.026
3.889IleAsp: 3.889 ± 0.067
4.134IleGlu: 4.134 ± 0.069
1.97IlePhe: 1.97 ± 0.052
4.738IleGly: 4.738 ± 0.072
1.249IleHis: 1.249 ± 0.037
3.029IleIle: 3.029 ± 0.067
2.71IleLys: 2.71 ± 0.058
5.483IleLeu: 5.483 ± 0.08
1.182IleMet: 1.182 ± 0.034
2.585IleAsn: 2.585 ± 0.064
2.589IlePro: 2.589 ± 0.058
2.224IleGln: 2.224 ± 0.046
3.016IleArg: 3.016 ± 0.058
3.932IleSer: 3.932 ± 0.07
3.139IleThr: 3.139 ± 0.071
3.982IleVal: 3.982 ± 0.069
0.681IleTrp: 0.681 ± 0.029
1.44IleTyr: 1.44 ± 0.042
0.0IleXaa: 0.0 ± 0.0
Lys
4.448LysAla: 4.448 ± 0.078
0.243LysCys: 0.243 ± 0.017
2.178LysAsp: 2.178 ± 0.053
2.081LysGlu: 2.081 ± 0.058
1.261LysPhe: 1.261 ± 0.036
2.582LysGly: 2.582 ± 0.064
0.85LysHis: 0.85 ± 0.031
2.96LysIle: 2.96 ± 0.056
2.408LysLys: 2.408 ± 0.071
4.657LysLeu: 4.657 ± 0.075
1.424LysMet: 1.424 ± 0.039
2.075LysAsn: 2.075 ± 0.046
2.298LysPro: 2.298 ± 0.055
2.18LysGln: 2.18 ± 0.052
2.117LysArg: 2.117 ± 0.059
2.599LysSer: 2.599 ± 0.056
2.766LysThr: 2.766 ± 0.056
3.081LysVal: 3.081 ± 0.065
0.452LysTrp: 0.452 ± 0.022
1.108LysTyr: 1.108 ± 0.037
0.0LysXaa: 0.0 ± 0.0
Leu
14.043LeuAla: 14.043 ± 0.167
1.118LeuCys: 1.118 ± 0.033
5.892LeuAsp: 5.892 ± 0.095
5.264LeuGlu: 5.264 ± 0.093
3.964LeuPhe: 3.964 ± 0.083
7.606LeuGly: 7.606 ± 0.103
2.212LeuHis: 2.212 ± 0.05
6.794LeuIle: 6.794 ± 0.083
4.972LeuLys: 4.972 ± 0.084
12.378LeuLeu: 12.378 ± 0.169
2.797LeuMet: 2.797 ± 0.056
4.574LeuAsn: 4.574 ± 0.071
5.667LeuPro: 5.667 ± 0.092
4.961LeuGln: 4.961 ± 0.092
5.765LeuArg: 5.765 ± 0.085
7.559LeuSer: 7.559 ± 0.113
5.69LeuThr: 5.69 ± 0.097
6.716LeuVal: 6.716 ± 0.098
1.26LeuTrp: 1.26 ± 0.041
2.399LeuTyr: 2.399 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.933MetAla: 2.933 ± 0.064
0.165MetCys: 0.165 ± 0.011
1.165MetAsp: 1.165 ± 0.033
0.894MetGlu: 0.894 ± 0.036
0.749MetPhe: 0.749 ± 0.03
1.846MetGly: 1.846 ± 0.044
0.564MetHis: 0.564 ± 0.023
1.358MetIle: 1.358 ± 0.041
1.278MetLys: 1.278 ± 0.041
3.03MetLeu: 3.03 ± 0.062
0.754MetMet: 0.754 ± 0.03
1.105MetAsn: 1.105 ± 0.033
1.433MetPro: 1.433 ± 0.041
1.473MetGln: 1.473 ± 0.041
1.331MetArg: 1.331 ± 0.037
1.896MetSer: 1.896 ± 0.047
1.434MetThr: 1.434 ± 0.038
1.769MetVal: 1.769 ± 0.045
0.227MetTrp: 0.227 ± 0.015
0.473MetTyr: 0.473 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.844AsnAla: 3.844 ± 0.067
0.339AsnCys: 0.339 ± 0.017
2.068AsnAsp: 2.068 ± 0.072
1.972AsnGlu: 1.972 ± 0.048
1.282AsnPhe: 1.282 ± 0.04
2.833AsnGly: 2.833 ± 0.069
0.858AsnHis: 0.858 ± 0.034
2.118AsnIle: 2.118 ± 0.055
1.652AsnLys: 1.652 ± 0.048
3.799AsnLeu: 3.799 ± 0.064
0.88AsnMet: 0.88 ± 0.034
1.447AsnAsn: 1.447 ± 0.045
2.337AsnPro: 2.337 ± 0.058
1.923AsnGln: 1.923 ± 0.053
1.947AsnArg: 1.947 ± 0.045
2.11AsnSer: 2.11 ± 0.055
2.048AsnThr: 2.048 ± 0.056
2.305AsnVal: 2.305 ± 0.057
0.558AsnTrp: 0.558 ± 0.022
1.044AsnTyr: 1.044 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
4.913ProAla: 4.913 ± 0.084
0.374ProCys: 0.374 ± 0.02
2.397ProAsp: 2.397 ± 0.055
3.081ProGlu: 3.081 ± 0.058
1.702ProPhe: 1.702 ± 0.043
2.38ProGly: 2.38 ± 0.052
0.985ProHis: 0.985 ± 0.035
2.6ProIle: 2.6 ± 0.058
1.82ProLys: 1.82 ± 0.048
4.65ProLeu: 4.65 ± 0.08
1.1ProMet: 1.1 ± 0.032
1.832ProAsn: 1.832 ± 0.046
1.561ProPro: 1.561 ± 0.055
2.109ProGln: 2.109 ± 0.048
1.831ProArg: 1.831 ± 0.053
2.766ProSer: 2.766 ± 0.056
2.402ProThr: 2.402 ± 0.064
3.292ProVal: 3.292 ± 0.071
0.617ProTrp: 0.617 ± 0.025
1.171ProTyr: 1.171 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
5.714GlnAla: 5.714 ± 0.104
0.439GlnCys: 0.439 ± 0.02
1.978GlnAsp: 1.978 ± 0.04
1.909GlnGlu: 1.909 ± 0.055
1.995GlnPhe: 1.995 ± 0.046
3.046GlnGly: 3.046 ± 0.071
1.38GlnHis: 1.38 ± 0.044
3.267GlnIle: 3.267 ± 0.067
1.918GlnLys: 1.918 ± 0.046
6.342GlnLeu: 6.342 ± 0.12
1.298GlnMet: 1.298 ± 0.036
1.62GlnAsn: 1.62 ± 0.04
1.875GlnPro: 1.875 ± 0.046
3.57GlnGln: 3.57 ± 0.102
2.987GlnArg: 2.987 ± 0.066
2.821GlnSer: 2.821 ± 0.065
2.5GlnThr: 2.5 ± 0.057
3.255GlnVal: 3.255 ± 0.051
0.808GlnTrp: 0.808 ± 0.031
1.433GlnTyr: 1.433 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
4.769ArgAla: 4.769 ± 0.069
0.497ArgCys: 0.497 ± 0.023
2.784ArgAsp: 2.784 ± 0.059
3.113ArgGlu: 3.113 ± 0.064
2.438ArgPhe: 2.438 ± 0.047
3.049ArgGly: 3.049 ± 0.062
1.273ArgHis: 1.273 ± 0.041
3.19ArgIle: 3.19 ± 0.063
2.034ArgLys: 2.034 ± 0.055
6.017ArgLeu: 6.017 ± 0.099
1.326ArgMet: 1.326 ± 0.035
1.831ArgAsn: 1.831 ± 0.045
2.013ArgPro: 2.013 ± 0.048
2.952ArgGln: 2.952 ± 0.065
2.958ArgArg: 2.958 ± 0.067
2.895ArgSer: 2.895 ± 0.063
2.237ArgThr: 2.237 ± 0.052
3.543ArgVal: 3.543 ± 0.07
0.832ArgTrp: 0.832 ± 0.033
1.749ArgTyr: 1.749 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
6.497SerAla: 6.497 ± 0.09
0.575SerCys: 0.575 ± 0.026
3.084SerAsp: 3.084 ± 0.066
3.161SerGlu: 3.161 ± 0.063
2.311SerPhe: 2.311 ± 0.054
4.813SerGly: 4.813 ± 0.077
1.372SerHis: 1.372 ± 0.04
3.502SerIle: 3.502 ± 0.073
2.621SerLys: 2.621 ± 0.068
6.538SerLeu: 6.538 ± 0.097
1.502SerMet: 1.502 ± 0.043
2.397SerAsn: 2.397 ± 0.056
2.656SerPro: 2.656 ± 0.054
2.983SerGln: 2.983 ± 0.06
3.041SerArg: 3.041 ± 0.06
3.979SerSer: 3.979 ± 0.082
3.146SerThr: 3.146 ± 0.062
4.056SerVal: 4.056 ± 0.08
0.821SerTrp: 0.821 ± 0.027
1.523SerTyr: 1.523 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
5.643ThrAla: 5.643 ± 0.099
0.432ThrCys: 0.432 ± 0.02
2.393ThrAsp: 2.393 ± 0.061
2.69ThrGlu: 2.69 ± 0.062
1.7ThrPhe: 1.7 ± 0.049
3.946ThrGly: 3.946 ± 0.079
1.173ThrHis: 1.173 ± 0.04
2.99ThrIle: 2.99 ± 0.078
1.528ThrLys: 1.528 ± 0.044
6.397ThrLeu: 6.397 ± 0.1
1.161ThrMet: 1.161 ± 0.037
1.406ThrAsn: 1.406 ± 0.046
3.284ThrPro: 3.284 ± 0.087
2.583ThrGln: 2.583 ± 0.053
2.393ThrArg: 2.393 ± 0.059
2.779ThrSer: 2.779 ± 0.063
2.668ThrThr: 2.668 ± 0.054
3.589ThrVal: 3.589 ± 0.125
0.711ThrTrp: 0.711 ± 0.025
1.2ThrTyr: 1.2 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
7.651ValAla: 7.651 ± 0.091
0.686ValCys: 0.686 ± 0.026
3.728ValAsp: 3.728 ± 0.076
3.741ValGlu: 3.741 ± 0.071
2.707ValPhe: 2.707 ± 0.056
4.823ValGly: 4.823 ± 0.084
1.253ValHis: 1.253 ± 0.036
4.246ValIle: 4.246 ± 0.072
3.037ValLys: 3.037 ± 0.063
7.771ValLeu: 7.771 ± 0.104
1.93ValMet: 1.93 ± 0.046
2.689ValAsn: 2.689 ± 0.084
2.702ValPro: 2.702 ± 0.048
2.601ValGln: 2.601 ± 0.056
3.251ValArg: 3.251 ± 0.063
4.262ValSer: 4.262 ± 0.068
3.641ValThr: 3.641 ± 0.096
5.175ValVal: 5.175 ± 0.085
0.854ValTrp: 0.854 ± 0.031
1.554ValTyr: 1.554 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.146TrpAla: 1.146 ± 0.036
0.172TrpCys: 0.172 ± 0.013
0.539TrpAsp: 0.539 ± 0.025
0.514TrpGlu: 0.514 ± 0.02
0.563TrpPhe: 0.563 ± 0.026
0.873TrpGly: 0.873 ± 0.035
0.385TrpHis: 0.385 ± 0.02
0.731TrpIle: 0.731 ± 0.027
0.436TrpLys: 0.436 ± 0.022
2.128TrpLeu: 2.128 ± 0.05
0.318TrpMet: 0.318 ± 0.019
0.423TrpAsn: 0.423 ± 0.022
0.619TrpPro: 0.619 ± 0.029
1.233TrpGln: 1.233 ± 0.047
1.023TrpArg: 1.023 ± 0.037
0.706TrpSer: 0.706 ± 0.028
0.534TrpThr: 0.534 ± 0.024
0.959TrpVal: 0.959 ± 0.033
0.262TrpTrp: 0.262 ± 0.017
0.339TrpTyr: 0.339 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.626TyrAla: 2.626 ± 0.054
0.336TyrCys: 0.336 ± 0.021
1.304TyrAsp: 1.304 ± 0.039
1.108TyrGlu: 1.108 ± 0.033
1.247TyrPhe: 1.247 ± 0.032
1.957TyrGly: 1.957 ± 0.045
0.6TyrHis: 0.6 ± 0.026
1.274TyrIle: 1.274 ± 0.038
0.881TyrLys: 0.881 ± 0.033
3.046TyrLeu: 3.046 ± 0.066
0.473TyrMet: 0.473 ± 0.022
0.806TyrAsn: 0.806 ± 0.034
1.324TyrPro: 1.324 ± 0.038
1.575TyrGln: 1.575 ± 0.042
1.72TyrArg: 1.72 ± 0.049
1.59TyrSer: 1.59 ± 0.046
1.371TyrThr: 1.371 ± 0.072
1.58TyrVal: 1.58 ± 0.043
0.431TyrTrp: 0.431 ± 0.021
0.727TyrTyr: 0.727 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3066 proteins (984057 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski