Sebaldella termitidis (strain ATCC 33386 / NCTC 11300)

Taxonomy: cellular organisms; Bacteria; Fusobacteria; Fusobacteriia; Fusobacteriales; Leptotrichiaceae; Sebaldella; Sebaldella termitidis

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1AKT0|D1AKT0_SEBTE Cell envelope-related transcriptional attenuator OS=Sebaldella termitidis (strain ATCC 33386 / NCTC 11300) OX=526218 GN=Sterm_0211 PE=3 SV=1
MM1 pKa = 7.73GLFLDD6 pKa = 5.34LGILKK11 pKa = 10.29NCDD14 pKa = 3.09KK15 pKa = 11.02IKK17 pKa = 10.72AKK19 pKa = 10.5KK20 pKa = 10.03ILDD23 pKa = 3.18AWAKK27 pKa = 10.81DD28 pKa = 3.49RR29 pKa = 11.84TSDD32 pKa = 4.2ISPDD36 pKa = 3.21EE37 pKa = 4.45CQLDD41 pKa = 4.02EE42 pKa = 4.44QNGDD46 pKa = 3.59TVILLNADD54 pKa = 3.7SNFEE58 pKa = 3.92GNAQFFKK65 pKa = 10.77YY66 pKa = 10.44ASSKK70 pKa = 9.82TNDD73 pKa = 3.19AVMSLYY79 pKa = 10.08IYY81 pKa = 10.9DD82 pKa = 3.75GDD84 pKa = 3.66YY85 pKa = 10.4WGYY88 pKa = 10.99NLFDD92 pKa = 4.03NGQEE96 pKa = 3.66IDD98 pKa = 4.29KK99 pKa = 10.64FNPLPDD105 pKa = 3.74YY106 pKa = 10.9FDD108 pKa = 6.53DD109 pKa = 4.01IDD111 pKa = 3.71EE112 pKa = 4.82SEE114 pKa = 3.98IEE116 pKa = 4.22AYY118 pKa = 9.7KK119 pKa = 10.98GNSDD123 pKa = 4.17IVAQYY128 pKa = 10.65FNINKK133 pKa = 10.04DD134 pKa = 3.42SIEE137 pKa = 4.11NYY139 pKa = 10.44LIFWTDD145 pKa = 2.88EE146 pKa = 3.93LMDD149 pKa = 4.29EE150 pKa = 4.36GTAYY154 pKa = 10.39EE155 pKa = 3.97NDD157 pKa = 3.16EE158 pKa = 4.48FGYY161 pKa = 10.84EE162 pKa = 3.88DD163 pKa = 3.43WQLVDD168 pKa = 4.97FMEE171 pKa = 4.5KK172 pKa = 10.21LGYY175 pKa = 10.26KK176 pKa = 10.05YY177 pKa = 10.64FEE179 pKa = 4.19

Molecular weight: 20.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1AL57|D1AL57_SEBTE MATE efflux family protein OS=Sebaldella termitidis (strain ATCC 33386 / NCTC 11300) OX=526218 GN=Sterm_2347 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.15KK3 pKa = 10.58LYY5 pKa = 9.39MVLILSLAVTSCVVVEE21 pKa = 4.18DD22 pKa = 5.01GYY24 pKa = 10.9RR25 pKa = 11.84GTRR28 pKa = 11.84VRR30 pKa = 11.84VRR32 pKa = 11.84RR33 pKa = 11.84PPLIIIGG40 pKa = 3.76

Molecular weight: 4.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4124

0

4124

1275552

30

4897

309.3

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.772 ± 0.051

0.735 ± 0.019

5.497 ± 0.028

7.705 ± 0.074

4.595 ± 0.051

7.305 ± 0.101

1.271 ± 0.016

9.262 ± 0.038

8.921 ± 0.079

8.795 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.024

6.576 ± 0.083

2.662 ± 0.021

2.203 ± 0.017

3.256 ± 0.038

6.361 ± 0.059

5.414 ± 0.077

6.06 ± 0.031

0.725 ± 0.013

4.417 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski