Pseudodesulfovibrio mercurii
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3451 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F0JG37|F0JG37_9DELT Pyruvate carboxylase OS=Pseudodesulfovibrio mercurii OX=641491 GN=DND132_0569 PE=4 SV=1
MM1 pKa = 7.69 NIRR4 pKa = 11.84 RR5 pKa = 11.84 FLWLVVLCFCLVTSEE20 pKa = 5.1 AFANPSITLYY30 pKa = 10.91 DD31 pKa = 3.17 WAFNIDD37 pKa = 3.8 GVITTSGDD45 pKa = 3.51 MPVSGSLTDD54 pKa = 4.01 GLGEE58 pKa = 4.39 LSWSTSEE65 pKa = 4.58 AGSHH69 pKa = 4.8 TFIAFFDD76 pKa = 4.08 YY77 pKa = 11.15 EE78 pKa = 4.0 IDD80 pKa = 3.73 EE81 pKa = 4.53 PLNTFFNEE89 pKa = 3.5 YY90 pKa = 9.1 GAAVGVPAPGMMWEE104 pKa = 3.82 IDD106 pKa = 3.48 EE107 pKa = 4.91 PGFLFGDD114 pKa = 4.14 IYY116 pKa = 10.4 THH118 pKa = 5.93 VLAGALDD125 pKa = 3.86 NTNALPWGNDD135 pKa = 3.17 DD136 pKa = 4.98 DD137 pKa = 5.54 VSFALGWTFSLAEE150 pKa = 3.96 NQIANIILDD159 pKa = 4.04 LSEE162 pKa = 5.94 LIPLVPFYY170 pKa = 10.34 LAQMDD175 pKa = 4.13 TQSPEE180 pKa = 3.74 TIYY183 pKa = 11.15 YY184 pKa = 10.25 SGALTIEE191 pKa = 4.36 TTPVPEE197 pKa = 4.68 PSTIILFGLGLLSALGVARR216 pKa = 11.84 ARR218 pKa = 11.84 ARR220 pKa = 11.84 RR221 pKa = 11.84 RR222 pKa = 3.31
Molecular weight: 24.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.605
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.656
EMBOSS 3.719
Sillero 3.923
Patrickios 0.998
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|F0JBL9|F0JBL9_9DELT Extracellular solute-binding protein family 3 OS=Pseudodesulfovibrio mercurii OX=641491 GN=DND132_2318 PE=4 SV=1
MM1 pKa = 7.7 PKK3 pKa = 9.95 IKK5 pKa = 9.47 TRR7 pKa = 11.84 RR8 pKa = 11.84 AAAKK12 pKa = 9.92 RR13 pKa = 11.84 FSQTATGKK21 pKa = 10.13 FKK23 pKa = 10.78 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 KK27 pKa = 9.07 NLRR30 pKa = 11.84 HH31 pKa = 6.24 ILTKK35 pKa = 10.67 KK36 pKa = 7.68 NAKK39 pKa = 9.22 RR40 pKa = 11.84 KK41 pKa = 9.18 RR42 pKa = 11.84 RR43 pKa = 11.84 LGQSTTVDD51 pKa = 3.53 TANMKK56 pKa = 10.08 AVRR59 pKa = 11.84 RR60 pKa = 11.84 QLPNGG65 pKa = 3.51
Molecular weight: 7.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.515
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.237
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.048
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3451
0
3451
1132766
31
2133
328.2
36.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.242 ± 0.05
1.305 ± 0.019
5.957 ± 0.038
6.35 ± 0.042
4.088 ± 0.028
8.35 ± 0.038
1.992 ± 0.019
5.096 ± 0.034
4.268 ± 0.042
10.63 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.844 ± 0.021
2.93 ± 0.02
4.928 ± 0.031
2.838 ± 0.024
6.608 ± 0.043
4.976 ± 0.026
5.178 ± 0.028
7.537 ± 0.031
1.203 ± 0.017
2.679 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here