Lactobacillus collinoides DSM 20515 = JCM 1123
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3157 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2B9U1|A0A0R2B9U1_LACCL Branched-chain amino acid ABC transporter substrate binding protein OS=Lactobacillus collinoides DSM 20515 = JCM 1123 OX=1423733 GN=FC82_GL002087 PE=3 SV=1
MM1 pKa = 8.04 DD2 pKa = 5.11 AGKK5 pKa = 8.0 TVPEE9 pKa = 4.31 TTKK12 pKa = 10.31 TPTGGISSASSNTDD26 pKa = 2.43 SSSILDD32 pKa = 3.71 EE33 pKa = 4.78 NKK35 pKa = 9.65 TEE37 pKa = 4.17 TPSVIDD43 pKa = 4.09 DD44 pKa = 3.82 VPGEE48 pKa = 4.14 VSGTPTIADD57 pKa = 3.23 DD58 pKa = 3.79 TLNVNKK64 pKa = 9.0 LTLINPTEE72 pKa = 4.17 KK73 pKa = 10.75 EE74 pKa = 3.03 IDD76 pKa = 3.42 AAKK79 pKa = 9.22 EE80 pKa = 4.09 TMEE83 pKa = 4.56 AAYY86 pKa = 9.69 KK87 pKa = 9.55 LTGQAQQLNALLDD100 pKa = 3.69 EE101 pKa = 5.0 AAPSASSAVITLTSNTDD118 pKa = 3.32 VIGYY122 pKa = 10.0 NSGQTSIILTFTVVGAKK139 pKa = 10.4 AGDD142 pKa = 3.89 VYY144 pKa = 10.91 TITIPKK150 pKa = 8.98 TSVFSYY156 pKa = 11.3 SSMDD160 pKa = 3.58 KK161 pKa = 10.21 LTATQGTSEE170 pKa = 4.32 TQSTADD176 pKa = 3.78 GGTMITVTMARR187 pKa = 11.84 SVSDD191 pKa = 3.44 LSFKK195 pKa = 10.65 IKK197 pKa = 10.64 LGTANNEE204 pKa = 4.07 NQQPRR209 pKa = 11.84 PMSDD213 pKa = 2.54 VGVNTYY219 pKa = 9.88 TITAMNGDD227 pKa = 4.12 TEE229 pKa = 4.49 VASIPITQTITPTISMGNVTRR250 pKa = 11.84 TTPNLSEE257 pKa = 4.06 LTAVQSGVDD266 pKa = 3.33 YY267 pKa = 10.85 IYY269 pKa = 10.33 TLKK272 pKa = 10.95 VNEE275 pKa = 4.28 ADD277 pKa = 3.35 GVYY280 pKa = 10.7 NDD282 pKa = 5.0 GSTSSYY288 pKa = 10.85 VNAAVNYY295 pKa = 7.06 GTTITIPVPEE305 pKa = 4.72 GFTLNSDD312 pKa = 3.61 VTNSINGFSDD322 pKa = 3.27 EE323 pKa = 4.45 TTISQPDD330 pKa = 3.7 GSGTNVIITVPKK342 pKa = 9.94 GSGKK346 pKa = 7.92 EE347 pKa = 4.11 TYY349 pKa = 9.95 EE350 pKa = 4.1 GGSGYY355 pKa = 10.89 KK356 pKa = 10.26 LVGSYY361 pKa = 10.71 DD362 pKa = 3.5 VAASEE367 pKa = 5.02 SEE369 pKa = 4.14 QTLTASGPVTITQVIDD385 pKa = 3.54 GTGTTITDD393 pKa = 3.13 EE394 pKa = 4.4 SEE396 pKa = 4.22 NVFSEE401 pKa = 4.19 TLAASTEE408 pKa = 4.06 IPDD411 pKa = 3.95 ASNISISMHH420 pKa = 5.94 GNSSSTSTEE429 pKa = 3.6 LALDD433 pKa = 3.99 GDD435 pKa = 4.34 TTNDD439 pKa = 3.21 PKK441 pKa = 11.52 YY442 pKa = 9.57 LTTYY446 pKa = 11.11 SLTDD450 pKa = 3.24 NSAISLTDD458 pKa = 3.86 DD459 pKa = 3.89 LNLTINVPDD468 pKa = 4.96 GIEE471 pKa = 4.07 MTSLTVPAEE480 pKa = 4.2 GATTSQYY487 pKa = 11.44 LPGTTSYY494 pKa = 11.31 AYY496 pKa = 10.49 QLTLADD502 pKa = 4.11 GSIEE506 pKa = 3.98 NGEE509 pKa = 3.95 VDD511 pKa = 3.78 AGGTITANYY520 pKa = 8.66 GSEE523 pKa = 3.82 VRR525 pKa = 11.84 QIVLTPNYY533 pKa = 9.16 LAAGAYY539 pKa = 7.02 TSSNAFILSGIMSSKK554 pKa = 10.71 YY555 pKa = 10.46 DD556 pKa = 3.87 DD557 pKa = 4.73 GSDD560 pKa = 3.39 VQYY563 pKa = 11.49 GDD565 pKa = 4.41 TIDD568 pKa = 4.01 FTSSLALSSDD578 pKa = 3.17 PSTIISSDD586 pKa = 3.75 SIKK589 pKa = 9.61 QTVIEE594 pKa = 4.53 SFAHH598 pKa = 6.24 FYY600 pKa = 10.94 AYY602 pKa = 10.78 LSQSDD607 pKa = 3.93 KK608 pKa = 11.31 TPGAVAAGSLSLRR621 pKa = 11.84 MTGQDD626 pKa = 2.41 SHH628 pKa = 5.45 TTYY631 pKa = 10.74 EE632 pKa = 3.97 VYY634 pKa = 10.58 EE635 pKa = 4.4 PIFYY639 pKa = 10.66 YY640 pKa = 10.46 VLPSATYY647 pKa = 9.33 ISGFSAIDD655 pKa = 3.31 SKK657 pKa = 11.97 AKK659 pKa = 9.03 VTEE662 pKa = 4.39 FQADD666 pKa = 3.53 DD667 pKa = 3.64 GRR669 pKa = 11.84 TVVQIDD675 pKa = 3.61 YY676 pKa = 10.35 SGTGEE681 pKa = 4.12 YY682 pKa = 10.92 VSTNSTFSQQVKK694 pKa = 9.75 LSNYY698 pKa = 9.97 ADD700 pKa = 4.48 ALPGSYY706 pKa = 10.4 GYY708 pKa = 11.46 LMYY711 pKa = 10.56 VYY713 pKa = 10.88 SPVTKK718 pKa = 10.45 LANTKK723 pKa = 9.56 MISDD727 pKa = 4.27 TSYY730 pKa = 10.73 SAGNAEE736 pKa = 3.85 AVEE739 pKa = 4.56 FGSGNWTISSASTFDD754 pKa = 3.29 QYY756 pKa = 11.99 SFGQTNEE763 pKa = 3.96 DD764 pKa = 3.5 VQGVTNATSDD774 pKa = 3.78 DD775 pKa = 3.95 QGSSDD780 pKa = 3.15 ATFYY784 pKa = 10.96 VALVNTLRR792 pKa = 11.84 DD793 pKa = 3.72 GTDD796 pKa = 2.9 NTTTSIINLPTSDD809 pKa = 4.24 DD810 pKa = 4.11 ANGSGYY816 pKa = 8.74 TFNLTGPVTLPTNFITATGTGDD838 pKa = 3.69 TIDD841 pKa = 3.54 ATVLYY846 pKa = 9.04 STATVAVTQGQKK858 pKa = 9.74 TIDD861 pKa = 3.26 TSSYY865 pKa = 9.44 VSADD869 pKa = 3.35 EE870 pKa = 4.14 VTSWDD875 pKa = 3.79 SIRR878 pKa = 11.84 SVLIKK883 pKa = 10.38 INNFGLDD890 pKa = 3.48 DD891 pKa = 3.99 STGKK895 pKa = 10.04 IALNGTIADD904 pKa = 4.02 MQNLAGMTGYY914 pKa = 10.23 LQAIVLVNDD923 pKa = 4.67 DD924 pKa = 4.18 GASVDD929 pKa = 3.54 NSTSLSITGTSTVTARR945 pKa = 11.84 FHH947 pKa = 6.9 YY948 pKa = 10.31 IDD950 pKa = 5.76 DD951 pKa = 4.18 NGEE954 pKa = 3.93 DD955 pKa = 3.63 QYY957 pKa = 11.12 ITLDD961 pKa = 3.84 DD962 pKa = 4.72 LKK964 pKa = 10.55 QTDD967 pKa = 3.82 LQDD970 pKa = 4.12 NVDD973 pKa = 4.35 SLQDD977 pKa = 3.42 IYY979 pKa = 10.35 PTNLDD984 pKa = 3.27 GFSAVDD990 pKa = 3.2 QALIPEE996 pKa = 5.57 GYY998 pKa = 8.25 TLASNQYY1005 pKa = 8.93 TYY1007 pKa = 11.68 VSGANADD1014 pKa = 4.51 GIMSSATTKK1023 pKa = 10.81 VGDD1026 pKa = 3.6 TAVYY1030 pKa = 8.51 NTDD1033 pKa = 3.4 GEE1035 pKa = 4.49 IVQYY1039 pKa = 10.61 EE1040 pKa = 4.15 LAKK1043 pKa = 10.6 NASLTVTYY1051 pKa = 10.23 IDD1053 pKa = 4.56 DD1054 pKa = 4.26 AADD1057 pKa = 3.76 GAVVGDD1063 pKa = 3.74 VTTVTGLLGASDD1075 pKa = 4.13 TYY1077 pKa = 11.28 KK1078 pKa = 10.72 VVIPNGYY1085 pKa = 9.35 EE1086 pKa = 3.86 LASGQASSIGYY1097 pKa = 7.03 TLSDD1101 pKa = 3.62 SDD1103 pKa = 3.85 TDD1105 pKa = 3.43 NLTVHH1110 pKa = 7.03 LVHH1113 pKa = 6.83 QITVIGTKK1121 pKa = 7.55 TTTNTVTYY1129 pKa = 9.7 TGLPTDD1135 pKa = 4.34 KK1136 pKa = 10.69 TPASQSKK1143 pKa = 8.84 KK1144 pKa = 9.29 VSWNVEE1150 pKa = 3.69 KK1151 pKa = 10.95 DD1152 pKa = 3.56 DD1153 pKa = 3.67 VTGEE1157 pKa = 4.04 VTYY1160 pKa = 10.86 VPSSTHH1166 pKa = 3.5 QTTYY1170 pKa = 7.75 TTTNVSGYY1178 pKa = 10.71 YY1179 pKa = 9.55 YY1180 pKa = 10.47 DD1181 pKa = 4.33 SSQAQVGFTVTTQTTEE1197 pKa = 4.02 PKK1199 pKa = 10.05 DD1200 pKa = 3.48 QSIEE1204 pKa = 3.74 VAYY1207 pKa = 10.53 YY1208 pKa = 11.16 AEE1210 pKa = 4.2 TQTTNIEE1217 pKa = 4.65 FVDD1220 pKa = 4.28 DD1221 pKa = 4.55 DD1222 pKa = 4.31 NNSAVVGTQTVLQGKK1237 pKa = 7.04 TDD1239 pKa = 3.79 EE1240 pKa = 4.21 NVPWSVSVPDD1250 pKa = 4.03 NYY1252 pKa = 10.85 VLASGQSSSGTYY1264 pKa = 7.57 TFKK1267 pKa = 11.15 ASDD1270 pKa = 3.53 NDD1272 pKa = 3.78 NIIVHH1277 pKa = 6.81 LSHH1280 pKa = 6.82 IQATGTTTTTRR1291 pKa = 11.84 NIYY1294 pKa = 10.46 FIVEE1298 pKa = 4.33 YY1299 pKa = 10.72 NNSLNPEE1306 pKa = 4.26 PVNQTAMWNVTKK1318 pKa = 11.05 DD1319 pKa = 3.46 MVTGAITATTTDD1331 pKa = 3.44 SYY1333 pKa = 11.66 NQVDD1337 pKa = 4.14 SLPISGYY1344 pKa = 6.55 TANTAIVPSEE1354 pKa = 3.96 NLEE1357 pKa = 4.65 PITTLPANAADD1368 pKa = 3.55 IYY1370 pKa = 9.95 VTYY1373 pKa = 9.3 TADD1376 pKa = 3.28 AQTASTEE1383 pKa = 4.13 YY1384 pKa = 10.96 VDD1386 pKa = 5.94 DD1387 pKa = 4.34 SASEE1391 pKa = 4.49 TIVGTPKK1398 pKa = 10.36 IIDD1401 pKa = 4.43 GYY1403 pKa = 9.78 TGTSTNWDD1411 pKa = 3.7 TSDD1414 pKa = 4.71 LPAGYY1419 pKa = 9.72 QLAANQPSSGTYY1431 pKa = 9.59 TFTAEE1436 pKa = 4.24 DD1437 pKa = 3.6 NQVVQIHH1444 pKa = 5.48 VVHH1447 pKa = 6.58 IHH1449 pKa = 5.38 TQSEE1453 pKa = 4.78 MSTKK1457 pKa = 10.06 DD1458 pKa = 2.99 TVNYY1462 pKa = 10.6 YY1463 pKa = 9.89 EE1464 pKa = 4.84 LPQDD1468 pKa = 3.64 KK1469 pKa = 10.94 AEE1471 pKa = 4.0 QSKK1474 pKa = 8.83 TEE1476 pKa = 4.09 SVNWKK1481 pKa = 9.65 IDD1483 pKa = 3.3 TDD1485 pKa = 3.79 EE1486 pKa = 4.25 ATGISTFTPSSTAIEE1501 pKa = 4.1 ISTPEE1506 pKa = 3.91 VPGYY1510 pKa = 8.97 TPSEE1514 pKa = 3.9 QTIKK1518 pKa = 9.75 FTQATSEE1525 pKa = 4.33 TQPSDD1530 pKa = 3.0 QTADD1534 pKa = 3.33 VTYY1537 pKa = 10.65 SADD1540 pKa = 3.46 PQEE1543 pKa = 4.09 ITFTYY1548 pKa = 10.82 VDD1550 pKa = 4.25 DD1551 pKa = 4.05 TTGDD1555 pKa = 3.26 EE1556 pKa = 4.53 MEE1558 pKa = 4.84 NLDD1561 pKa = 3.8 ITLEE1565 pKa = 4.09 GHH1567 pKa = 5.14 TDD1569 pKa = 3.12 EE1570 pKa = 5.76 AGTYY1574 pKa = 7.63 TVIVPKK1580 pKa = 10.63 NYY1582 pKa = 9.97 VLSQGQSATVPYY1594 pKa = 9.55 TYY1596 pKa = 9.9 TADD1599 pKa = 3.31 VQTPTKK1605 pKa = 10.61 VHH1607 pKa = 6.03 LTHH1610 pKa = 7.34 AIADD1614 pKa = 4.07 GTVVTTRR1621 pKa = 11.84 TITYY1625 pKa = 8.78 VVNGNPTAAPKK1636 pKa = 10.67 AVVQKK1641 pKa = 9.32 ITWKK1645 pKa = 10.1 VVRR1648 pKa = 11.84 DD1649 pKa = 3.84 MVTGTSYY1656 pKa = 10.23 ATAQNGYY1663 pKa = 9.76 DD1664 pKa = 3.46 AVITPEE1670 pKa = 4.04 LAGYY1674 pKa = 7.2 TADD1677 pKa = 3.78 KK1678 pKa = 11.04 SGIAQEE1684 pKa = 3.93 LLGRR1688 pKa = 11.84 VDD1690 pKa = 3.6 TADD1693 pKa = 3.96 LADD1696 pKa = 3.72 SAVTVTYY1703 pKa = 8.9 TAIPVAPDD1711 pKa = 3.15 NDD1713 pKa = 4.11 SEE1715 pKa = 4.85 NEE1717 pKa = 4.37 VVTPTQPDD1725 pKa = 3.72 TAVSGTMGSDD1735 pKa = 3.09 VDD1737 pKa = 4.57 NEE1739 pKa = 4.17 QSDD1742 pKa = 4.19 VTVLEE1747 pKa = 4.36 KK1748 pKa = 11.02 DD1749 pKa = 3.5 NQKK1752 pKa = 8.37 VTRR1755 pKa = 11.84 DD1756 pKa = 3.64 GNSDD1760 pKa = 3.42 EE1761 pKa = 4.54 TTKK1764 pKa = 11.22 SNAFGSSAGIYY1775 pKa = 8.58 VHH1777 pKa = 7.06 GSTHH1781 pKa = 6.75 ADD1783 pKa = 3.01 AVPTVTTDD1791 pKa = 3.15 DD1792 pKa = 3.71 TGVKK1796 pKa = 10.02 KK1797 pKa = 9.71 GTANKK1802 pKa = 10.11 VNITTAAKK1810 pKa = 10.33 LPQTDD1815 pKa = 3.68 EE1816 pKa = 4.22 KK1817 pKa = 11.46 QNGIWTIAGASLLGLFSLFGIGKK1840 pKa = 9.22 KK1841 pKa = 9.5 KK1842 pKa = 10.27 RR1843 pKa = 11.84 RR1844 pKa = 11.84 EE1845 pKa = 3.71 DD1846 pKa = 3.51 DD1847 pKa = 3.16
Molecular weight: 195.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.859
IPC_protein 3.897
Toseland 3.668
ProMoST 4.05
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.253
Thurlkill 3.719
EMBOSS 3.834
Sillero 4.024
Patrickios 0.985
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.9
Protein with the highest isoelectric point:
>tr|A0A0R2B817|A0A0R2B817_LACCL Glycosyltransferase OS=Lactobacillus collinoides DSM 20515 = JCM 1123 OX=1423733 GN=FC82_GL002417 PE=4 SV=1
MM1 pKa = 7.55 NILSQRR7 pKa = 11.84 MIDD10 pKa = 3.61 MSGHH14 pKa = 6.19 RR15 pKa = 11.84 IGRR18 pKa = 11.84 LVVIKK23 pKa = 10.61 RR24 pKa = 11.84 AGKK27 pKa = 9.95 LSNGNITWFCQCDD40 pKa = 3.57 CGNQCVVDD48 pKa = 3.66 GHH50 pKa = 6.27 LLRR53 pKa = 11.84 TGRR56 pKa = 11.84 TLSCGCIRR64 pKa = 11.84 KK65 pKa = 8.67 QRR67 pKa = 11.84 SRR69 pKa = 11.84 EE70 pKa = 4.07 LIRR73 pKa = 11.84 NNPKK77 pKa = 8.26 TRR79 pKa = 11.84 QYY81 pKa = 10.43 IGNWKK86 pKa = 9.07 PLEE89 pKa = 4.38 KK90 pKa = 9.12 TWHH93 pKa = 6.04 PRR95 pKa = 11.84 ATDD98 pKa = 3.22 RR99 pKa = 11.84 RR100 pKa = 11.84 KK101 pKa = 10.6 NNSSGITGVSYY112 pKa = 10.27 DD113 pKa = 3.72 QKK115 pKa = 7.87 QHH117 pKa = 5.08 RR118 pKa = 11.84 WIARR122 pKa = 11.84 LYY124 pKa = 9.44 FKK126 pKa = 10.81 GRR128 pKa = 11.84 YY129 pKa = 8.29 VLNKK133 pKa = 9.83 TFDD136 pKa = 3.56 NKK138 pKa = 10.96 ANAVEE143 pKa = 4.05 ARR145 pKa = 11.84 RR146 pKa = 11.84 QAEE149 pKa = 4.25 LRR151 pKa = 11.84 FLTLQEE157 pKa = 4.59 KK158 pKa = 10.7 NEE160 pKa = 3.97 QLL162 pKa = 3.5
Molecular weight: 18.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.663
IPC_protein 10.365
Toseland 10.774
ProMoST 10.423
Dawson 10.847
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 11.111
Grimsley 10.891
Solomon 10.965
Lehninger 10.935
Nozaki 10.774
DTASelect 10.54
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.833
IPC_peptide 10.979
IPC2_peptide 9.721
IPC2.peptide.svr19 8.596
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3157
0
3157
958480
50
3774
303.6
33.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.692 ± 0.047
0.485 ± 0.011
6.024 ± 0.044
4.87 ± 0.041
4.187 ± 0.031
6.919 ± 0.035
2.287 ± 0.023
6.701 ± 0.046
5.757 ± 0.045
9.7 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.691 ± 0.023
4.567 ± 0.034
3.705 ± 0.024
4.484 ± 0.037
3.972 ± 0.036
6.051 ± 0.049
7.039 ± 0.078
7.341 ± 0.037
1.069 ± 0.015
3.456 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here