Lactobacillus collinoides DSM 20515 = JCM 1123

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Secundilactobacillus; Secundilactobacillus collinoides

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0R2B9U1|A0A0R2B9U1_LACCL Branched-chain amino acid ABC transporter substrate binding protein OS=Lactobacillus collinoides DSM 20515 = JCM 1123 OX=1423733 GN=FC82_GL002087 PE=3 SV=1
MM1 pKa = 8.04DD2 pKa = 5.11AGKK5 pKa = 8.0TVPEE9 pKa = 4.31TTKK12 pKa = 10.31TPTGGISSASSNTDD26 pKa = 2.43SSSILDD32 pKa = 3.71EE33 pKa = 4.78NKK35 pKa = 9.65TEE37 pKa = 4.17TPSVIDD43 pKa = 4.09DD44 pKa = 3.82VPGEE48 pKa = 4.14VSGTPTIADD57 pKa = 3.23DD58 pKa = 3.79TLNVNKK64 pKa = 9.0LTLINPTEE72 pKa = 4.17KK73 pKa = 10.75EE74 pKa = 3.03IDD76 pKa = 3.42AAKK79 pKa = 9.22EE80 pKa = 4.09TMEE83 pKa = 4.56AAYY86 pKa = 9.69KK87 pKa = 9.55LTGQAQQLNALLDD100 pKa = 3.69EE101 pKa = 5.0AAPSASSAVITLTSNTDD118 pKa = 3.32VIGYY122 pKa = 10.0NSGQTSIILTFTVVGAKK139 pKa = 10.4AGDD142 pKa = 3.89VYY144 pKa = 10.91TITIPKK150 pKa = 8.98TSVFSYY156 pKa = 11.3SSMDD160 pKa = 3.58KK161 pKa = 10.21LTATQGTSEE170 pKa = 4.32TQSTADD176 pKa = 3.78GGTMITVTMARR187 pKa = 11.84SVSDD191 pKa = 3.44LSFKK195 pKa = 10.65IKK197 pKa = 10.64LGTANNEE204 pKa = 4.07NQQPRR209 pKa = 11.84PMSDD213 pKa = 2.54VGVNTYY219 pKa = 9.88TITAMNGDD227 pKa = 4.12TEE229 pKa = 4.49VASIPITQTITPTISMGNVTRR250 pKa = 11.84TTPNLSEE257 pKa = 4.06LTAVQSGVDD266 pKa = 3.33YY267 pKa = 10.85IYY269 pKa = 10.33TLKK272 pKa = 10.95VNEE275 pKa = 4.28ADD277 pKa = 3.35GVYY280 pKa = 10.7NDD282 pKa = 5.0GSTSSYY288 pKa = 10.85VNAAVNYY295 pKa = 7.06GTTITIPVPEE305 pKa = 4.72GFTLNSDD312 pKa = 3.61VTNSINGFSDD322 pKa = 3.27EE323 pKa = 4.45TTISQPDD330 pKa = 3.7GSGTNVIITVPKK342 pKa = 9.94GSGKK346 pKa = 7.92EE347 pKa = 4.11TYY349 pKa = 9.95EE350 pKa = 4.1GGSGYY355 pKa = 10.89KK356 pKa = 10.26LVGSYY361 pKa = 10.71DD362 pKa = 3.5VAASEE367 pKa = 5.02SEE369 pKa = 4.14QTLTASGPVTITQVIDD385 pKa = 3.54GTGTTITDD393 pKa = 3.13EE394 pKa = 4.4SEE396 pKa = 4.22NVFSEE401 pKa = 4.19TLAASTEE408 pKa = 4.06IPDD411 pKa = 3.95ASNISISMHH420 pKa = 5.94GNSSSTSTEE429 pKa = 3.6LALDD433 pKa = 3.99GDD435 pKa = 4.34TTNDD439 pKa = 3.21PKK441 pKa = 11.52YY442 pKa = 9.57LTTYY446 pKa = 11.11SLTDD450 pKa = 3.24NSAISLTDD458 pKa = 3.86DD459 pKa = 3.89LNLTINVPDD468 pKa = 4.96GIEE471 pKa = 4.07MTSLTVPAEE480 pKa = 4.2GATTSQYY487 pKa = 11.44LPGTTSYY494 pKa = 11.31AYY496 pKa = 10.49QLTLADD502 pKa = 4.11GSIEE506 pKa = 3.98NGEE509 pKa = 3.95VDD511 pKa = 3.78AGGTITANYY520 pKa = 8.66GSEE523 pKa = 3.82VRR525 pKa = 11.84QIVLTPNYY533 pKa = 9.16LAAGAYY539 pKa = 7.02TSSNAFILSGIMSSKK554 pKa = 10.71YY555 pKa = 10.46DD556 pKa = 3.87DD557 pKa = 4.73GSDD560 pKa = 3.39VQYY563 pKa = 11.49GDD565 pKa = 4.41TIDD568 pKa = 4.01FTSSLALSSDD578 pKa = 3.17PSTIISSDD586 pKa = 3.75SIKK589 pKa = 9.61QTVIEE594 pKa = 4.53SFAHH598 pKa = 6.24FYY600 pKa = 10.94AYY602 pKa = 10.78LSQSDD607 pKa = 3.93KK608 pKa = 11.31TPGAVAAGSLSLRR621 pKa = 11.84MTGQDD626 pKa = 2.41SHH628 pKa = 5.45TTYY631 pKa = 10.74EE632 pKa = 3.97VYY634 pKa = 10.58EE635 pKa = 4.4PIFYY639 pKa = 10.66YY640 pKa = 10.46VLPSATYY647 pKa = 9.33ISGFSAIDD655 pKa = 3.31SKK657 pKa = 11.97AKK659 pKa = 9.03VTEE662 pKa = 4.39FQADD666 pKa = 3.53DD667 pKa = 3.64GRR669 pKa = 11.84TVVQIDD675 pKa = 3.61YY676 pKa = 10.35SGTGEE681 pKa = 4.12YY682 pKa = 10.92VSTNSTFSQQVKK694 pKa = 9.75LSNYY698 pKa = 9.97ADD700 pKa = 4.48ALPGSYY706 pKa = 10.4GYY708 pKa = 11.46LMYY711 pKa = 10.56VYY713 pKa = 10.88SPVTKK718 pKa = 10.45LANTKK723 pKa = 9.56MISDD727 pKa = 4.27TSYY730 pKa = 10.73SAGNAEE736 pKa = 3.85AVEE739 pKa = 4.56FGSGNWTISSASTFDD754 pKa = 3.29QYY756 pKa = 11.99SFGQTNEE763 pKa = 3.96DD764 pKa = 3.5VQGVTNATSDD774 pKa = 3.78DD775 pKa = 3.95QGSSDD780 pKa = 3.15ATFYY784 pKa = 10.96VALVNTLRR792 pKa = 11.84DD793 pKa = 3.72GTDD796 pKa = 2.9NTTTSIINLPTSDD809 pKa = 4.24DD810 pKa = 4.11ANGSGYY816 pKa = 8.74TFNLTGPVTLPTNFITATGTGDD838 pKa = 3.69TIDD841 pKa = 3.54ATVLYY846 pKa = 9.04STATVAVTQGQKK858 pKa = 9.74TIDD861 pKa = 3.26TSSYY865 pKa = 9.44VSADD869 pKa = 3.35EE870 pKa = 4.14VTSWDD875 pKa = 3.79SIRR878 pKa = 11.84SVLIKK883 pKa = 10.38INNFGLDD890 pKa = 3.48DD891 pKa = 3.99STGKK895 pKa = 10.04IALNGTIADD904 pKa = 4.02MQNLAGMTGYY914 pKa = 10.23LQAIVLVNDD923 pKa = 4.67DD924 pKa = 4.18GASVDD929 pKa = 3.54NSTSLSITGTSTVTARR945 pKa = 11.84FHH947 pKa = 6.9YY948 pKa = 10.31IDD950 pKa = 5.76DD951 pKa = 4.18NGEE954 pKa = 3.93DD955 pKa = 3.63QYY957 pKa = 11.12ITLDD961 pKa = 3.84DD962 pKa = 4.72LKK964 pKa = 10.55QTDD967 pKa = 3.82LQDD970 pKa = 4.12NVDD973 pKa = 4.35SLQDD977 pKa = 3.42IYY979 pKa = 10.35PTNLDD984 pKa = 3.27GFSAVDD990 pKa = 3.2QALIPEE996 pKa = 5.57GYY998 pKa = 8.25TLASNQYY1005 pKa = 8.93TYY1007 pKa = 11.68VSGANADD1014 pKa = 4.51GIMSSATTKK1023 pKa = 10.81VGDD1026 pKa = 3.6TAVYY1030 pKa = 8.51NTDD1033 pKa = 3.4GEE1035 pKa = 4.49IVQYY1039 pKa = 10.61EE1040 pKa = 4.15LAKK1043 pKa = 10.6NASLTVTYY1051 pKa = 10.23IDD1053 pKa = 4.56DD1054 pKa = 4.26AADD1057 pKa = 3.76GAVVGDD1063 pKa = 3.74VTTVTGLLGASDD1075 pKa = 4.13TYY1077 pKa = 11.28KK1078 pKa = 10.72VVIPNGYY1085 pKa = 9.35EE1086 pKa = 3.86LASGQASSIGYY1097 pKa = 7.03TLSDD1101 pKa = 3.62SDD1103 pKa = 3.85TDD1105 pKa = 3.43NLTVHH1110 pKa = 7.03LVHH1113 pKa = 6.83QITVIGTKK1121 pKa = 7.55TTTNTVTYY1129 pKa = 9.7TGLPTDD1135 pKa = 4.34KK1136 pKa = 10.69TPASQSKK1143 pKa = 8.84KK1144 pKa = 9.29VSWNVEE1150 pKa = 3.69KK1151 pKa = 10.95DD1152 pKa = 3.56DD1153 pKa = 3.67VTGEE1157 pKa = 4.04VTYY1160 pKa = 10.86VPSSTHH1166 pKa = 3.5QTTYY1170 pKa = 7.75TTTNVSGYY1178 pKa = 10.71YY1179 pKa = 9.55YY1180 pKa = 10.47DD1181 pKa = 4.33SSQAQVGFTVTTQTTEE1197 pKa = 4.02PKK1199 pKa = 10.05DD1200 pKa = 3.48QSIEE1204 pKa = 3.74VAYY1207 pKa = 10.53YY1208 pKa = 11.16AEE1210 pKa = 4.2TQTTNIEE1217 pKa = 4.65FVDD1220 pKa = 4.28DD1221 pKa = 4.55DD1222 pKa = 4.31NNSAVVGTQTVLQGKK1237 pKa = 7.04TDD1239 pKa = 3.79EE1240 pKa = 4.21NVPWSVSVPDD1250 pKa = 4.03NYY1252 pKa = 10.85VLASGQSSSGTYY1264 pKa = 7.57TFKK1267 pKa = 11.15ASDD1270 pKa = 3.53NDD1272 pKa = 3.78NIIVHH1277 pKa = 6.81LSHH1280 pKa = 6.82IQATGTTTTTRR1291 pKa = 11.84NIYY1294 pKa = 10.46FIVEE1298 pKa = 4.33YY1299 pKa = 10.72NNSLNPEE1306 pKa = 4.26PVNQTAMWNVTKK1318 pKa = 11.05DD1319 pKa = 3.46MVTGAITATTTDD1331 pKa = 3.44SYY1333 pKa = 11.66NQVDD1337 pKa = 4.14SLPISGYY1344 pKa = 6.55TANTAIVPSEE1354 pKa = 3.96NLEE1357 pKa = 4.65PITTLPANAADD1368 pKa = 3.55IYY1370 pKa = 9.95VTYY1373 pKa = 9.3TADD1376 pKa = 3.28AQTASTEE1383 pKa = 4.13YY1384 pKa = 10.96VDD1386 pKa = 5.94DD1387 pKa = 4.34SASEE1391 pKa = 4.49TIVGTPKK1398 pKa = 10.36IIDD1401 pKa = 4.43GYY1403 pKa = 9.78TGTSTNWDD1411 pKa = 3.7TSDD1414 pKa = 4.71LPAGYY1419 pKa = 9.72QLAANQPSSGTYY1431 pKa = 9.59TFTAEE1436 pKa = 4.24DD1437 pKa = 3.6NQVVQIHH1444 pKa = 5.48VVHH1447 pKa = 6.58IHH1449 pKa = 5.38TQSEE1453 pKa = 4.78MSTKK1457 pKa = 10.06DD1458 pKa = 2.99TVNYY1462 pKa = 10.6YY1463 pKa = 9.89EE1464 pKa = 4.84LPQDD1468 pKa = 3.64KK1469 pKa = 10.94AEE1471 pKa = 4.0QSKK1474 pKa = 8.83TEE1476 pKa = 4.09SVNWKK1481 pKa = 9.65IDD1483 pKa = 3.3TDD1485 pKa = 3.79EE1486 pKa = 4.25ATGISTFTPSSTAIEE1501 pKa = 4.1ISTPEE1506 pKa = 3.91VPGYY1510 pKa = 8.97TPSEE1514 pKa = 3.9QTIKK1518 pKa = 9.75FTQATSEE1525 pKa = 4.33TQPSDD1530 pKa = 3.0QTADD1534 pKa = 3.33VTYY1537 pKa = 10.65SADD1540 pKa = 3.46PQEE1543 pKa = 4.09ITFTYY1548 pKa = 10.82VDD1550 pKa = 4.25DD1551 pKa = 4.05TTGDD1555 pKa = 3.26EE1556 pKa = 4.53MEE1558 pKa = 4.84NLDD1561 pKa = 3.8ITLEE1565 pKa = 4.09GHH1567 pKa = 5.14TDD1569 pKa = 3.12EE1570 pKa = 5.76AGTYY1574 pKa = 7.63TVIVPKK1580 pKa = 10.63NYY1582 pKa = 9.97VLSQGQSATVPYY1594 pKa = 9.55TYY1596 pKa = 9.9TADD1599 pKa = 3.31VQTPTKK1605 pKa = 10.61VHH1607 pKa = 6.03LTHH1610 pKa = 7.34AIADD1614 pKa = 4.07GTVVTTRR1621 pKa = 11.84TITYY1625 pKa = 8.78VVNGNPTAAPKK1636 pKa = 10.67AVVQKK1641 pKa = 9.32ITWKK1645 pKa = 10.1VVRR1648 pKa = 11.84DD1649 pKa = 3.84MVTGTSYY1656 pKa = 10.23ATAQNGYY1663 pKa = 9.76DD1664 pKa = 3.46AVITPEE1670 pKa = 4.04LAGYY1674 pKa = 7.2TADD1677 pKa = 3.78KK1678 pKa = 11.04SGIAQEE1684 pKa = 3.93LLGRR1688 pKa = 11.84VDD1690 pKa = 3.6TADD1693 pKa = 3.96LADD1696 pKa = 3.72SAVTVTYY1703 pKa = 8.9TAIPVAPDD1711 pKa = 3.15NDD1713 pKa = 4.11SEE1715 pKa = 4.85NEE1717 pKa = 4.37VVTPTQPDD1725 pKa = 3.72TAVSGTMGSDD1735 pKa = 3.09VDD1737 pKa = 4.57NEE1739 pKa = 4.17QSDD1742 pKa = 4.19VTVLEE1747 pKa = 4.36KK1748 pKa = 11.02DD1749 pKa = 3.5NQKK1752 pKa = 8.37VTRR1755 pKa = 11.84DD1756 pKa = 3.64GNSDD1760 pKa = 3.42EE1761 pKa = 4.54TTKK1764 pKa = 11.22SNAFGSSAGIYY1775 pKa = 8.58VHH1777 pKa = 7.06GSTHH1781 pKa = 6.75ADD1783 pKa = 3.01AVPTVTTDD1791 pKa = 3.15DD1792 pKa = 3.71TGVKK1796 pKa = 10.02KK1797 pKa = 9.71GTANKK1802 pKa = 10.11VNITTAAKK1810 pKa = 10.33LPQTDD1815 pKa = 3.68EE1816 pKa = 4.22KK1817 pKa = 11.46QNGIWTIAGASLLGLFSLFGIGKK1840 pKa = 9.22KK1841 pKa = 9.5KK1842 pKa = 10.27RR1843 pKa = 11.84RR1844 pKa = 11.84EE1845 pKa = 3.71DD1846 pKa = 3.51DD1847 pKa = 3.16

Molecular weight:
195.32 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0R2B817|A0A0R2B817_LACCL Glycosyltransferase OS=Lactobacillus collinoides DSM 20515 = JCM 1123 OX=1423733 GN=FC82_GL002417 PE=4 SV=1
MM1 pKa = 7.55NILSQRR7 pKa = 11.84MIDD10 pKa = 3.61MSGHH14 pKa = 6.19RR15 pKa = 11.84IGRR18 pKa = 11.84LVVIKK23 pKa = 10.61RR24 pKa = 11.84AGKK27 pKa = 9.95LSNGNITWFCQCDD40 pKa = 3.57CGNQCVVDD48 pKa = 3.66GHH50 pKa = 6.27LLRR53 pKa = 11.84TGRR56 pKa = 11.84TLSCGCIRR64 pKa = 11.84KK65 pKa = 8.67QRR67 pKa = 11.84SRR69 pKa = 11.84EE70 pKa = 4.07LIRR73 pKa = 11.84NNPKK77 pKa = 8.26TRR79 pKa = 11.84QYY81 pKa = 10.43IGNWKK86 pKa = 9.07PLEE89 pKa = 4.38KK90 pKa = 9.12TWHH93 pKa = 6.04PRR95 pKa = 11.84ATDD98 pKa = 3.22RR99 pKa = 11.84RR100 pKa = 11.84KK101 pKa = 10.6NNSSGITGVSYY112 pKa = 10.27DD113 pKa = 3.72QKK115 pKa = 7.87QHH117 pKa = 5.08RR118 pKa = 11.84WIARR122 pKa = 11.84LYY124 pKa = 9.44FKK126 pKa = 10.81GRR128 pKa = 11.84YY129 pKa = 8.29VLNKK133 pKa = 9.83TFDD136 pKa = 3.56NKK138 pKa = 10.96ANAVEE143 pKa = 4.05ARR145 pKa = 11.84RR146 pKa = 11.84QAEE149 pKa = 4.25LRR151 pKa = 11.84FLTLQEE157 pKa = 4.59KK158 pKa = 10.7NEE160 pKa = 3.97QLL162 pKa = 3.5

Molecular weight:
18.95 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3157

0

3157

958480

50

3774

303.6

33.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.692 ± 0.047

0.485 ± 0.011

6.024 ± 0.044

4.87 ± 0.041

4.187 ± 0.031

6.919 ± 0.035

2.287 ± 0.023

6.701 ± 0.046

5.757 ± 0.045

9.7 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.023

4.567 ± 0.034

3.705 ± 0.024

4.484 ± 0.037

3.972 ± 0.036

6.051 ± 0.049

7.039 ± 0.078

7.341 ± 0.037

1.069 ± 0.015

3.456 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski