Pasteurellaceae bacterium 15-036681

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; unclassified Pasteurellaceae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2341 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V3TSB7|A0A1V3TSB7_9PAST UPF0181 protein BMT54_05560 OS=Pasteurellaceae bacterium 15-036681 OX=1924934 GN=BMT54_05560 PE=3 SV=1
MM1 pKa = 7.33AVVGLFYY8 pKa = 11.0GSDD11 pKa = 3.2TGNTEE16 pKa = 4.52NISKK20 pKa = 9.68MIQKK24 pKa = 9.57QLGSNLVDD32 pKa = 3.01IRR34 pKa = 11.84DD35 pKa = 3.58IAKK38 pKa = 8.47STKK41 pKa = 9.86EE42 pKa = 4.18DD43 pKa = 3.05IEE45 pKa = 5.8AYY47 pKa = 10.37DD48 pKa = 3.8FLLFGIPTWYY58 pKa = 10.38YY59 pKa = 11.33GEE61 pKa = 4.66SQADD65 pKa = 3.34WDD67 pKa = 4.88DD68 pKa = 4.41FMPTLKK74 pKa = 10.77EE75 pKa = 3.27IDD77 pKa = 3.87FNGKK81 pKa = 8.82VVGIFGCGDD90 pKa = 3.21QEE92 pKa = 5.04DD93 pKa = 4.3YY94 pKa = 11.79AEE96 pKa = 4.32YY97 pKa = 10.5FCDD100 pKa = 4.05AMGTVRR106 pKa = 11.84DD107 pKa = 3.9VVEE110 pKa = 4.2PNGGVLVGLWPTEE123 pKa = 3.88GYY125 pKa = 8.73TFEE128 pKa = 5.07ASQALVDD135 pKa = 4.61DD136 pKa = 4.2DD137 pKa = 4.8TFVGLCIDD145 pKa = 4.19EE146 pKa = 4.82DD147 pKa = 3.9RR148 pKa = 11.84QPEE151 pKa = 4.24LTEE154 pKa = 3.78EE155 pKa = 5.16RR156 pKa = 11.84VNTWCKK162 pKa = 10.46QIFDD166 pKa = 4.13EE167 pKa = 4.5MCLAEE172 pKa = 4.38LAA174 pKa = 4.24

Molecular weight: 19.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V3TWI8|A0A1V3TWI8_9PAST High-affinity zinc uptake system protein ZnuA OS=Pasteurellaceae bacterium 15-036681 OX=1924934 GN=BMT54_00525 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84ARR14 pKa = 11.84SHH16 pKa = 6.09GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.76VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84QSLSAA44 pKa = 4.02

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2341

0

2341

739913

25

4196

316.1

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.381 ± 0.055

0.953 ± 0.022

5.184 ± 0.05

6.5 ± 0.054

4.324 ± 0.043

6.748 ± 0.049

1.965 ± 0.026

6.935 ± 0.052

6.217 ± 0.048

10.133 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.027

4.908 ± 0.052

3.707 ± 0.039

4.803 ± 0.054

4.287 ± 0.048

6.034 ± 0.041

5.392 ± 0.062

6.87 ± 0.046

1.124 ± 0.019

3.155 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski