Wuhan millipede virus 3
Average proteome isoelectric point is 5.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KM91|A0A1L3KM91_9VIRU Uncharacterized protein OS=Wuhan millipede virus 3 OX=1923742 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 10.36 NATNQSITEE11 pKa = 3.97 QTEE14 pKa = 3.66 EE15 pKa = 4.09 QIQITHH21 pKa = 6.78 LEE23 pKa = 4.36 DD24 pKa = 3.5 EE25 pKa = 5.04 GSTEE29 pKa = 3.95 QVVASLVNTNIDD41 pKa = 3.78 SQSLQSTVGEE51 pKa = 4.48 LRR53 pKa = 11.84 NHH55 pKa = 7.69 DD56 pKa = 3.37 IPDD59 pKa = 3.61 FLEE62 pKa = 3.94 RR63 pKa = 11.84 LYY65 pKa = 10.86 EE66 pKa = 4.15 LEE68 pKa = 4.1 SFTWTKK74 pKa = 10.22 TSVRR78 pKa = 11.84 GQALKK83 pKa = 9.95 TYY85 pKa = 9.26 RR86 pKa = 11.84 FPDD89 pKa = 3.47 ALLNLAPIAAKK100 pKa = 9.17 TRR102 pKa = 11.84 NFFGFRR108 pKa = 11.84 AGVEE112 pKa = 3.98 LVVLVNKK119 pKa = 10.19 QPFQAGNLMISYY131 pKa = 9.77 LPNARR136 pKa = 11.84 YY137 pKa = 7.57 NTLKK141 pKa = 10.61 HH142 pKa = 6.22 DD143 pKa = 3.66 LHH145 pKa = 6.76 RR146 pKa = 11.84 RR147 pKa = 11.84 NEE149 pKa = 4.53 GIVMRR154 pKa = 11.84 SGCPRR159 pKa = 11.84 TNLDD163 pKa = 3.95 LMDD166 pKa = 3.93 GTRR169 pKa = 11.84 ANLEE173 pKa = 4.04 VPYY176 pKa = 10.41 VSPFVYY182 pKa = 10.81 YY183 pKa = 11.0 NLLTKK188 pKa = 10.41 EE189 pKa = 4.02 GTIGDD194 pKa = 4.16 FAISVYY200 pKa = 11.07 SPLTDD205 pKa = 3.25 VAASGTVSVQVMARR219 pKa = 11.84 FIKK222 pKa = 10.48 PEE224 pKa = 3.87 LAFPTGLVPATYY236 pKa = 10.45 NPLSEE241 pKa = 4.76 ISSQLLRR248 pKa = 11.84 VDD250 pKa = 3.44 TEE252 pKa = 4.04 DD253 pKa = 3.22 RR254 pKa = 11.84 AKK256 pKa = 10.21 IRR258 pKa = 11.84 KK259 pKa = 8.42 AQTEE263 pKa = 4.25 LQKK266 pKa = 11.38 LLDD269 pKa = 4.93 KK270 pKa = 10.8 IDD272 pKa = 3.32 GTMAHH277 pKa = 5.96 QVGNQNTMNFKK288 pKa = 10.5 QKK290 pKa = 10.81 ALPNMTNSDD299 pKa = 3.29 GSEE302 pKa = 4.0 FSHH305 pKa = 6.5 NLSISKK311 pKa = 10.48 NNSLKK316 pKa = 10.7 SASLGHH322 pKa = 6.87 AGPSEE327 pKa = 3.95 MDD329 pKa = 3.0 IKK331 pKa = 10.85 HH332 pKa = 6.26 ILAIPCFHH340 pKa = 7.13 NYY342 pKa = 9.39 FNISKK347 pKa = 8.91 SQAGGTSVFSTLVGPTVLPNYY368 pKa = 8.6 TSTLGTPIDD377 pKa = 3.91 YY378 pKa = 11.27 LNFFANMFQKK388 pKa = 9.79 WRR390 pKa = 11.84 GSIKK394 pKa = 9.97 YY395 pKa = 9.51 SFRR398 pKa = 11.84 AVKK401 pKa = 8.53 TQFHH405 pKa = 6.16 SCRR408 pKa = 11.84 IRR410 pKa = 11.84 VWFCPGATEE419 pKa = 4.55 QSGVDD424 pKa = 3.45 RR425 pKa = 11.84 NACYY429 pKa = 10.74 SKK431 pKa = 10.57 IVDD434 pKa = 4.06 LKK436 pKa = 10.71 EE437 pKa = 4.09 LNTFEE442 pKa = 4.87 FEE444 pKa = 4.56 VPFVYY449 pKa = 9.65 PQPWLNTFTAPNSLGVLGVDD469 pKa = 3.5 ILNALVAPDD478 pKa = 3.97 TVADD482 pKa = 3.79 NFDD485 pKa = 4.15 VIVEE489 pKa = 4.32 RR490 pKa = 11.84 SMGSDD495 pKa = 3.2 FAFNLVEE502 pKa = 4.82 PNTVMPRR509 pKa = 11.84 DD510 pKa = 3.61 ASTHH514 pKa = 6.18 KK515 pKa = 10.41 DD516 pKa = 3.23 TVASFSGKK524 pKa = 9.1 FRR526 pKa = 11.84 HH527 pKa = 6.64 QIGTQSNQDD536 pKa = 3.38 NQRR539 pKa = 11.84 TLQDD543 pKa = 4.79 DD544 pKa = 3.66 ITFEE548 pKa = 4.52 RR549 pKa = 11.84 PVSSVWADD557 pKa = 3.47 EE558 pKa = 4.08 HH559 pKa = 7.08 TMGQDD564 pKa = 2.76 ITNVKK569 pKa = 9.76 QLIGRR574 pKa = 11.84 ATRR577 pKa = 11.84 FSRR580 pKa = 11.84 YY581 pKa = 9.26 IYY583 pKa = 8.24 TIPDD587 pKa = 3.14 ATQSFNIAAGTGTIAYY603 pKa = 9.6 ASATNTTDD611 pKa = 3.63 FTGTAFADD619 pKa = 4.78 FKK621 pKa = 11.32 LPTGGSLTVNATKK634 pKa = 10.66 SFAVVYY640 pKa = 10.45 QGTHH644 pKa = 4.9 YY645 pKa = 11.34 ALAPGEE651 pKa = 3.91 YY652 pKa = 9.53 RR653 pKa = 11.84 ITDD656 pKa = 3.29 KK657 pKa = 11.34 TDD659 pKa = 3.25 FFGIHH664 pKa = 5.34 MTNFGDD670 pKa = 4.61 PEE672 pKa = 4.15 MQLVTKK678 pKa = 10.53 SDD680 pKa = 3.74 FTTAPATTAFPEE692 pKa = 4.14 ITVLGTVEE700 pKa = 4.17 EE701 pKa = 4.26 EE702 pKa = 4.01 QLALTIYY709 pKa = 6.6 PHH711 pKa = 6.77 CMGVAKK717 pKa = 10.13 WDD719 pKa = 4.12 DD720 pKa = 3.8 NNNLYY725 pKa = 11.07 ANSFDD730 pKa = 3.06 ILTYY734 pKa = 10.68 LSSLYY739 pKa = 9.38 TFQRR743 pKa = 11.84 GGVNIKK749 pKa = 10.03 IINALEE755 pKa = 4.48 DD756 pKa = 3.34 YY757 pKa = 10.81 AIALNPEE764 pKa = 3.44 QSYY767 pKa = 10.21 VNKK770 pKa = 10.06 AAEE773 pKa = 4.2 RR774 pKa = 11.84 VTLTNTSKK782 pKa = 11.59 ANMSSAIIQEE792 pKa = 3.78 VRR794 pKa = 11.84 PLVEE798 pKa = 4.81 GYY800 pKa = 11.05 GEE802 pKa = 3.9 IHH804 pKa = 6.49 VPFYY808 pKa = 11.32 SNSYY812 pKa = 8.17 CTSINNASLFTPLDD826 pKa = 3.77 EE827 pKa = 4.92 VNNLCMPATAITLIANGPVPQIQFYY852 pKa = 10.75 RR853 pKa = 11.84 SASPDD858 pKa = 3.24 FEE860 pKa = 4.33 FSYY863 pKa = 11.31 LCGTPLII870 pKa = 4.68
Molecular weight: 96.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.264
IPC2_protein 5.296
IPC_protein 5.296
Toseland 5.448
ProMoST 5.525
Dawson 5.397
Bjellqvist 5.486
Wikipedia 5.334
Rodwell 5.347
Grimsley 5.474
Solomon 5.397
Lehninger 5.372
Nozaki 5.575
DTASelect 5.753
Thurlkill 5.55
EMBOSS 5.512
Sillero 5.664
Patrickios 3.872
IPC_peptide 5.41
IPC2_peptide 5.664
IPC2.peptide.svr19 5.614
Protein with the highest isoelectric point:
>tr|A0A1L3KM91|A0A1L3KM91_9VIRU Uncharacterized protein OS=Wuhan millipede virus 3 OX=1923742 PE=4 SV=1
MM1 pKa = 7.38 ATTTEE6 pKa = 4.35 INPSALKK13 pKa = 9.48 ATLKK17 pKa = 9.99 QMKK20 pKa = 9.25 TIRR23 pKa = 11.84 QKK25 pKa = 10.85 FDD27 pKa = 3.22 DD28 pKa = 4.02 VSRR31 pKa = 11.84 LEE33 pKa = 4.45 HH34 pKa = 6.8 VLEE37 pKa = 3.94 AHH39 pKa = 7.0 RR40 pKa = 11.84 NQSKK44 pKa = 9.65 MSKK47 pKa = 9.25 PKK49 pKa = 9.78 KK50 pKa = 9.68 DD51 pKa = 3.35 PRR53 pKa = 11.84 FLEE56 pKa = 4.34 EE57 pKa = 3.83 KK58 pKa = 10.52 RR59 pKa = 11.84 EE60 pKa = 4.15 GKK62 pKa = 9.23 MKK64 pKa = 9.19 TQNFNRR70 pKa = 11.84 KK71 pKa = 8.35 VDD73 pKa = 3.3 IKK75 pKa = 10.29 RR76 pKa = 11.84 WKK78 pKa = 8.72 TINEE82 pKa = 4.19 LEE84 pKa = 4.2 YY85 pKa = 10.42 QHH87 pKa = 6.82 QIFDD91 pKa = 4.55 GIKK94 pKa = 9.75 SFFGKK99 pKa = 10.79 VNDD102 pKa = 4.57 FGDD105 pKa = 4.47 KK106 pKa = 10.27 IPEE109 pKa = 3.67 FGMRR113 pKa = 11.84 LDD115 pKa = 3.62 EE116 pKa = 4.92 ASAKK120 pKa = 10.05 LAEE123 pKa = 4.36 GMKK126 pKa = 10.4 DD127 pKa = 3.85 FPEE130 pKa = 4.44 TMQKK134 pKa = 11.09 ANDD137 pKa = 3.4 ATTTVSRR144 pKa = 11.84 ALDD147 pKa = 3.54 MIYY150 pKa = 10.69 QLMEE154 pKa = 3.71 TAKK157 pKa = 11.18 DD158 pKa = 3.44 KK159 pKa = 7.99 MTKK162 pKa = 9.86 ISDD165 pKa = 4.52 GITGFFEE172 pKa = 3.91 QLKK175 pKa = 6.6 MHH177 pKa = 7.13 PYY179 pKa = 9.24 IVAITFIILIVLMTQPFKK197 pKa = 11.03 YY198 pKa = 10.27 AVVPLVLFIMYY209 pKa = 10.5 LLGWHH214 pKa = 6.35 EE215 pKa = 4.37 KK216 pKa = 10.17 IIAKK220 pKa = 9.51 VKK222 pKa = 10.34 QILARR227 pKa = 11.84 FRR229 pKa = 11.84 QQDD232 pKa = 3.39 GVTDD236 pKa = 4.5 SISIFGQIIFTILAFFGISQIPTEE260 pKa = 4.44 KK261 pKa = 10.31 FYY263 pKa = 10.58 EE264 pKa = 4.21 TLIKK268 pKa = 10.61 RR269 pKa = 11.84 LDD271 pKa = 4.29 SIPKK275 pKa = 9.9 AFTGATKK282 pKa = 9.9 IWDD285 pKa = 3.82 SAGKK289 pKa = 10.08 AFEE292 pKa = 4.3 VVSDD296 pKa = 3.51 QFKK299 pKa = 9.23 ITFLHH304 pKa = 6.32 YY305 pKa = 10.24 KK306 pKa = 10.47 AEE308 pKa = 4.18 DD309 pKa = 4.13 LIKK312 pKa = 10.93 EE313 pKa = 4.17 EE314 pKa = 4.39 GTVRR318 pKa = 11.84 EE319 pKa = 3.94 IMVWSTRR326 pKa = 11.84 VLHH329 pKa = 6.1 YY330 pKa = 9.53 MQVPNRR336 pKa = 11.84 TSLSKK341 pKa = 11.07 DD342 pKa = 3.0 EE343 pKa = 4.13 EE344 pKa = 4.28 AVRR347 pKa = 11.84 EE348 pKa = 4.18 VTEE351 pKa = 5.29 LFNQMYY357 pKa = 9.36 RR358 pKa = 11.84 WQHH361 pKa = 4.45 TTSIWKK367 pKa = 9.66 SLPTEE372 pKa = 4.04 SQRR375 pKa = 11.84 LILSQKK381 pKa = 10.24 PHH383 pKa = 5.66 MQDD386 pKa = 3.2 LFRR389 pKa = 11.84 IVCKK393 pKa = 10.69 SSVHH397 pKa = 5.81 EE398 pKa = 4.39 GGPRR402 pKa = 11.84 KK403 pKa = 10.02 APISILFSGEE413 pKa = 3.72 SGRR416 pKa = 11.84 GKK418 pKa = 10.4 SEE420 pKa = 3.82 LLIPLAYY427 pKa = 10.35 ALLDD431 pKa = 3.55 NRR433 pKa = 11.84 GTKK436 pKa = 8.71 TNHH439 pKa = 5.78 RR440 pKa = 11.84 NEE442 pKa = 4.04 IYY444 pKa = 10.04 VRR446 pKa = 11.84 NYY448 pKa = 7.12 EE449 pKa = 3.91 TEE451 pKa = 3.97 YY452 pKa = 9.92 WDD454 pKa = 4.87 GYY456 pKa = 9.95 CGQKK460 pKa = 9.76 IVLFDD465 pKa = 4.84 DD466 pKa = 4.46 AFQMKK471 pKa = 8.03 DD472 pKa = 3.14 TPGNPSPEE480 pKa = 3.75 FMEE483 pKa = 5.15 AIRR486 pKa = 11.84 VNNTAPAHH494 pKa = 4.58 VHH496 pKa = 6.37 CADD499 pKa = 3.88 TNDD502 pKa = 3.28 KK503 pKa = 11.12 GRR505 pKa = 11.84 FFSSEE510 pKa = 3.18 ICLYY514 pKa = 7.09 TTNLQKK520 pKa = 11.14 DD521 pKa = 4.02 FTRR524 pKa = 11.84 FIASMNCPEE533 pKa = 4.08 AAVRR537 pKa = 11.84 RR538 pKa = 11.84 LNMNAFRR545 pKa = 11.84 IITNPAYY552 pKa = 10.13 EE553 pKa = 4.42 KK554 pKa = 10.65 VIQVDD559 pKa = 3.85 GQEE562 pKa = 4.1 VRR564 pKa = 11.84 RR565 pKa = 11.84 LDD567 pKa = 3.45 PTLCGWSKK575 pKa = 10.55 HH576 pKa = 5.62 RR577 pKa = 11.84 EE578 pKa = 3.85 QLKK581 pKa = 9.05 CRR583 pKa = 11.84 CYY585 pKa = 10.92 GEE587 pKa = 5.11 YY588 pKa = 9.88 FDD590 pKa = 6.41 SMGLKK595 pKa = 8.47 HH596 pKa = 6.45 TGTGKK601 pKa = 10.38 DD602 pKa = 3.64 PACIAHH608 pKa = 7.19 PCQKK612 pKa = 10.58 CKK614 pKa = 10.55 DD615 pKa = 3.13 ICIRR619 pKa = 11.84 EE620 pKa = 4.11 NMDD623 pKa = 3.35 EE624 pKa = 4.54 LAFCPHH630 pKa = 6.95 HH631 pKa = 6.64 YY632 pKa = 10.58 LFQRR636 pKa = 11.84 YY637 pKa = 8.5 NMLTDD642 pKa = 3.6 EE643 pKa = 5.26 SIGEE647 pKa = 4.14 PLDD650 pKa = 3.65 FTALLAILRR659 pKa = 11.84 TYY661 pKa = 10.2 DD662 pKa = 3.19 QNLVARR668 pKa = 11.84 EE669 pKa = 3.86 TGKK672 pKa = 10.84 LDD674 pKa = 3.72 FYY676 pKa = 11.53 EE677 pKa = 4.91 KK678 pKa = 10.1 FASNIRR684 pKa = 11.84 LFEE687 pKa = 4.14 HH688 pKa = 6.15 QMGPNEE694 pKa = 3.85 EE695 pKa = 4.41 HH696 pKa = 6.09 QEE698 pKa = 3.84 QRR700 pKa = 11.84 APEE703 pKa = 3.94 VDD705 pKa = 3.32 EE706 pKa = 4.46 TFSMAHH712 pKa = 7.11 LYY714 pKa = 10.54 NFEE717 pKa = 4.29 DD718 pKa = 3.76 AFYY721 pKa = 10.77 QPGVVDD727 pKa = 5.0 LEE729 pKa = 4.47 VPEE732 pKa = 4.93 DD733 pKa = 3.57 ALSFHH738 pKa = 7.17 LMQNFILEE746 pKa = 4.09 QVRR749 pKa = 11.84 LYY751 pKa = 10.93 GHH753 pKa = 6.22 EE754 pKa = 3.8 RR755 pKa = 11.84 DD756 pKa = 3.91 FFGLLEE762 pKa = 4.33 VEE764 pKa = 4.86 LSYY767 pKa = 11.12 IPALYY772 pKa = 10.32 AFHH775 pKa = 7.31 RR776 pKa = 11.84 RR777 pKa = 11.84 ATEE780 pKa = 3.69 FGIININNINYY791 pKa = 10.33 SLINCLDD798 pKa = 3.62 AEE800 pKa = 4.66 EE801 pKa = 3.96 IVYY804 pKa = 9.03 MRR806 pKa = 11.84 EE807 pKa = 3.45 QAKK810 pKa = 9.37 FYY812 pKa = 10.43 CVKK815 pKa = 10.19 KK816 pKa = 9.86 QSCIRR821 pKa = 11.84 LLYY824 pKa = 9.37 EE825 pKa = 3.84 WARR828 pKa = 11.84 SYY830 pKa = 11.45 LSRR833 pKa = 11.84 TVGQLTSLLDD843 pKa = 3.73 EE844 pKa = 4.62 TPVTILLTNLTFFVGLAALIITTKK868 pKa = 10.84 NLFKK872 pKa = 10.89 SFHH875 pKa = 6.22 TSSKK879 pKa = 10.56 LDD881 pKa = 3.36 RR882 pKa = 11.84 LYY884 pKa = 10.66 KK885 pKa = 8.99 QEE887 pKa = 4.49 KK888 pKa = 9.03 IAVEE892 pKa = 3.45 QHH894 pKa = 4.84 IRR896 pKa = 11.84 EE897 pKa = 3.99 IQEE900 pKa = 4.32 IYY902 pKa = 9.71 WSARR906 pKa = 11.84 PKK908 pKa = 9.89 QQALFGSCAFGDD920 pKa = 3.64 ACEE923 pKa = 4.18 RR924 pKa = 11.84 APGEE928 pKa = 4.1 LDD930 pKa = 3.53 HH931 pKa = 7.28 PAALQILSSMCLVCCPSCYY950 pKa = 10.3 KK951 pKa = 9.8 MSHH954 pKa = 7.28 DD955 pKa = 3.7 GTIAASYY962 pKa = 10.84 KK963 pKa = 10.37 FGFDD967 pKa = 4.72 AEE969 pKa = 4.52 SQVYY973 pKa = 8.94 NANLLRR979 pKa = 11.84 EE980 pKa = 3.98 YY981 pKa = 9.79 WRR983 pKa = 11.84 RR984 pKa = 11.84 NSQTQHH990 pKa = 5.38 VAQSMPIQVIGTNMTIGTQEE1010 pKa = 3.75 INLDD1014 pKa = 3.65 DD1015 pKa = 4.9 SEE1017 pKa = 4.6 THH1019 pKa = 5.5 TKK1021 pKa = 9.68 IAAEE1025 pKa = 4.14 TAASSANVNIRR1036 pKa = 11.84 TVPLVKK1042 pKa = 10.46 VEE1044 pKa = 4.72 ASASSANVNLTTRR1057 pKa = 11.84 PQVRR1061 pKa = 11.84 TEE1063 pKa = 3.69 ITPTEE1068 pKa = 3.85 IAEE1071 pKa = 4.3 KK1072 pKa = 10.48 VVTGVKK1078 pKa = 9.54 EE1079 pKa = 3.5 NHH1081 pKa = 6.91 AIVRR1085 pKa = 11.84 WIEE1088 pKa = 3.95 DD1089 pKa = 3.7 KK1090 pKa = 10.46 ITDD1093 pKa = 3.82 RR1094 pKa = 11.84 KK1095 pKa = 10.18 VSSIEE1100 pKa = 3.71 RR1101 pKa = 11.84 KK1102 pKa = 9.09 IFTRR1106 pKa = 11.84 NKK1108 pKa = 8.29 EE1109 pKa = 3.98 RR1110 pKa = 11.84 KK1111 pKa = 9.81 LEE1113 pKa = 3.94 FDD1115 pKa = 3.48 NLALKK1120 pKa = 10.62 ALLGVLAKK1128 pKa = 10.61 DD1129 pKa = 4.03 DD1130 pKa = 4.66 ASHH1133 pKa = 6.8 EE1134 pKa = 4.37 MACGLNEE1141 pKa = 4.32 EE1142 pKa = 4.86 YY1143 pKa = 9.99 WDD1145 pKa = 3.88 QVNSGIKK1152 pKa = 8.6 TVEE1155 pKa = 3.33 IRR1157 pKa = 11.84 KK1158 pKa = 9.32 FIGKK1162 pKa = 6.92 WQRR1165 pKa = 11.84 LTVGEE1170 pKa = 4.19 IMEE1173 pKa = 4.35 IRR1175 pKa = 11.84 CGDD1178 pKa = 3.03 KK1179 pKa = 10.31 MMRR1182 pKa = 11.84 KK1183 pKa = 8.79 QVIALSVNKK1192 pKa = 10.54 LEE1194 pKa = 4.24 EE1195 pKa = 4.04 LLRR1198 pKa = 11.84 NCGGSAMPGFTLQEE1212 pKa = 3.83 ALTAYY1217 pKa = 10.36 SCIYY1221 pKa = 9.92 PDD1223 pKa = 3.25 WQGSEE1228 pKa = 4.99 FIAITLAEE1236 pKa = 4.66 PEE1238 pKa = 4.12 NSSYY1242 pKa = 10.37 LTTPKK1247 pKa = 10.34 EE1248 pKa = 3.95 LRR1250 pKa = 11.84 HH1251 pKa = 6.16 EE1252 pKa = 4.0 IHH1254 pKa = 7.0 ADD1256 pKa = 3.21 SNGRR1260 pKa = 11.84 QLVGRR1265 pKa = 11.84 IMRR1268 pKa = 11.84 NSLYY1272 pKa = 10.75 ALHH1275 pKa = 7.66 DD1276 pKa = 4.27 DD1277 pKa = 3.12 KK1278 pKa = 11.68 FMYY1281 pKa = 10.83 GNVLFVMGTTALMPWHH1297 pKa = 7.15 FIDD1300 pKa = 5.24 AMRR1303 pKa = 11.84 INKK1306 pKa = 8.52 NRR1308 pKa = 11.84 DD1309 pKa = 2.98 EE1310 pKa = 4.87 LLYY1313 pKa = 10.95 LSNLNGKK1320 pKa = 9.57 NIITFPASRR1329 pKa = 11.84 LDD1331 pKa = 3.46 NAVRR1335 pKa = 11.84 LQKK1338 pKa = 10.97 GGFDD1342 pKa = 3.33 TDD1344 pKa = 3.41 AALVRR1349 pKa = 11.84 FDD1351 pKa = 3.84 PVNDD1355 pKa = 3.76 KK1356 pKa = 10.56 VHH1358 pKa = 6.42 AHH1360 pKa = 6.05 PKK1362 pKa = 8.84 IIKK1365 pKa = 10.33 SFVSQSNLKK1374 pKa = 10.04 NFPEE1378 pKa = 4.13 SSKK1381 pKa = 10.67 FFAILSSYY1389 pKa = 9.54 RR1390 pKa = 11.84 TMSTLTHH1397 pKa = 7.24 FIPSMISCNDD1407 pKa = 2.94 VGAHH1411 pKa = 6.47 FDD1413 pKa = 3.37 ATNTINIDD1421 pKa = 3.64 GALQQYY1427 pKa = 9.9 KK1428 pKa = 10.26 YY1429 pKa = 10.36 RR1430 pKa = 11.84 DD1431 pKa = 3.09 IYY1433 pKa = 11.3 AYY1435 pKa = 10.57 FSDD1438 pKa = 4.36 TQFGDD1443 pKa = 3.36 CGAPLVLQNVNSDD1456 pKa = 3.46 QKK1458 pKa = 11.12 ILGIHH1463 pKa = 6.3 VAGQDD1468 pKa = 3.16 NGVAFSQCITAEE1480 pKa = 4.18 MIQEE1484 pKa = 5.36 GIAKK1488 pKa = 9.0 MPFAQQIFTEE1498 pKa = 4.82 PYY1500 pKa = 9.43 EE1501 pKa = 4.33 MIEE1504 pKa = 4.18 EE1505 pKa = 4.91 EE1506 pKa = 4.25 IPLDD1510 pKa = 3.61 AEE1512 pKa = 4.57 RR1513 pKa = 11.84 EE1514 pKa = 4.3 GSVPMGAGLNVIGKK1528 pKa = 7.5 MAPRR1532 pKa = 11.84 YY1533 pKa = 9.26 NIRR1536 pKa = 11.84 GATKK1540 pKa = 9.66 TKK1542 pKa = 8.7 ITPSVFFDD1550 pKa = 3.5 KK1551 pKa = 10.47 VTEE1554 pKa = 4.22 HH1555 pKa = 5.99 KK1556 pKa = 9.66 TMPTVLRR1563 pKa = 11.84 KK1564 pKa = 10.17 DD1565 pKa = 3.87 GPAGDD1570 pKa = 3.67 PMEE1573 pKa = 5.39 RR1574 pKa = 11.84 GLLKK1578 pKa = 10.48 FGKK1581 pKa = 9.43 SVPWIQPTLIKK1592 pKa = 10.69 VATNDD1597 pKa = 3.14 VEE1599 pKa = 4.29 NNYY1602 pKa = 10.52 NINACGLSPQQYY1614 pKa = 9.16 RR1615 pKa = 11.84 RR1616 pKa = 11.84 KK1617 pKa = 9.96 LDD1619 pKa = 3.31 YY1620 pKa = 11.21 CEE1622 pKa = 4.54 AVCGVPDD1629 pKa = 4.58 DD1630 pKa = 5.11 EE1631 pKa = 5.42 YY1632 pKa = 10.57 MAPINRR1638 pKa = 11.84 GTSLGFPYY1646 pKa = 10.56 VVDD1649 pKa = 3.65 WNHH1652 pKa = 6.49 PNGKK1656 pKa = 9.15 RR1657 pKa = 11.84 EE1658 pKa = 4.28 AFGTDD1663 pKa = 2.8 EE1664 pKa = 4.65 WKK1666 pKa = 10.95 LDD1668 pKa = 3.38 TPQAKK1673 pKa = 10.03 KK1674 pKa = 10.15 IEE1676 pKa = 4.21 RR1677 pKa = 11.84 DD1678 pKa = 3.57 VMEE1681 pKa = 5.87 LEE1683 pKa = 4.46 DD1684 pKa = 3.23 KK1685 pKa = 10.78 CRR1687 pKa = 11.84 RR1688 pKa = 11.84 GEE1690 pKa = 3.78 QAGVYY1695 pKa = 7.06 WTDD1698 pKa = 3.02 TLKK1701 pKa = 11.03 DD1702 pKa = 3.33 EE1703 pKa = 4.62 RR1704 pKa = 11.84 RR1705 pKa = 11.84 PLEE1708 pKa = 3.84 KK1709 pKa = 10.66 VKK1711 pKa = 10.81 AGKK1714 pKa = 8.06 TRR1716 pKa = 11.84 VFCAGPVHH1724 pKa = 5.58 FTILFRR1730 pKa = 11.84 MYY1732 pKa = 10.79 FLGFAAWIMRR1742 pKa = 11.84 NRR1744 pKa = 11.84 NSNEE1748 pKa = 3.14 ISTGTNVYY1756 pKa = 10.58 SYY1758 pKa = 10.86 DD1759 pKa = 3.25 WNNIARR1765 pKa = 11.84 KK1766 pKa = 9.18 LRR1768 pKa = 11.84 SRR1770 pKa = 11.84 GLDD1773 pKa = 2.97 SKK1775 pKa = 11.76 GNVGVVAGDD1784 pKa = 3.64 FEE1786 pKa = 5.2 NFDD1789 pKa = 4.43 GSLSSQILWAIFEE1802 pKa = 4.79 SIQRR1806 pKa = 11.84 WYY1808 pKa = 11.32 NDD1810 pKa = 3.27 GEE1812 pKa = 4.23 EE1813 pKa = 4.25 NEE1815 pKa = 4.44 MIRR1818 pKa = 11.84 RR1819 pKa = 11.84 TLWAHH1824 pKa = 6.46 LVHH1827 pKa = 6.65 AVHH1830 pKa = 6.36 VNEE1833 pKa = 4.07 GTVYY1837 pKa = 10.56 SATHH1841 pKa = 5.7 SQPSGCPITAILNSIYY1857 pKa = 10.6 NSIVIRR1863 pKa = 11.84 IVYY1866 pKa = 9.96 LLAAEE1871 pKa = 4.56 DD1872 pKa = 4.51 FEE1874 pKa = 5.68 INNNLRR1880 pKa = 11.84 LGEE1883 pKa = 4.21 LANMEE1888 pKa = 4.5 KK1889 pKa = 10.49 FNQFVACVSYY1899 pKa = 11.55 GDD1901 pKa = 4.72 DD1902 pKa = 3.52 NLIAICEE1909 pKa = 4.25 MIRR1912 pKa = 11.84 EE1913 pKa = 4.47 WFNQIIITEE1922 pKa = 4.09 KK1923 pKa = 9.79 FKK1925 pKa = 11.17 QIGHH1929 pKa = 6.46 VYY1931 pKa = 8.87 TDD1933 pKa = 3.27 EE1934 pKa = 4.59 RR1935 pKa = 11.84 KK1936 pKa = 7.86 TGEE1939 pKa = 3.91 IYY1941 pKa = 9.69 TVRR1944 pKa = 11.84 SLSEE1948 pKa = 3.68 VAFLKK1953 pKa = 10.73 RR1954 pKa = 11.84 KK1955 pKa = 8.58 FVWSEE1960 pKa = 3.24 MAQRR1964 pKa = 11.84 FIAPLDD1970 pKa = 3.35 IDD1972 pKa = 3.91 VVLEE1976 pKa = 3.74 IVQWTKK1982 pKa = 10.95 RR1983 pKa = 11.84 GMQKK1987 pKa = 10.85 DD1988 pKa = 4.5 EE1989 pKa = 3.8 ITLANIEE1996 pKa = 3.94 VCMRR2000 pKa = 11.84 EE2001 pKa = 3.97 LSLHH2005 pKa = 6.02 GEE2007 pKa = 4.17 NVYY2010 pKa = 11.09 NLYY2013 pKa = 10.83 KK2014 pKa = 10.79 NEE2016 pKa = 4.01 FKK2018 pKa = 10.65 HH2019 pKa = 5.03 VCAMHH2024 pKa = 6.88 GINFRR2029 pKa = 11.84 FLTWHH2034 pKa = 6.1 EE2035 pKa = 4.81 VYY2037 pKa = 10.73 CSVLEE2042 pKa = 4.14 LPLYY2046 pKa = 9.3 LHH2048 pKa = 6.8 EE2049 pKa = 5.5 GEE2051 pKa = 4.5 VTPLDD2056 pKa = 4.47 LMITNEE2062 pKa = 4.1 WVVHH2066 pKa = 6.28 CISADD2071 pKa = 3.08 AKK2073 pKa = 9.84 MSRR2076 pKa = 11.84 GFAKK2080 pKa = 10.64 ALVAEE2085 pKa = 4.89 RR2086 pKa = 11.84 MTQTEE2091 pKa = 4.2 VEE2093 pKa = 4.16 RR2094 pKa = 11.84 LKK2096 pKa = 10.76 KK2097 pKa = 10.2 AKK2099 pKa = 10.31 HH2100 pKa = 6.04 EE2101 pKa = 4.24 IGDD2104 pKa = 4.08 VIFCDD2109 pKa = 4.01 DD2110 pKa = 4.01 SKK2112 pKa = 10.19 TIHH2115 pKa = 6.69 LVTKK2119 pKa = 9.81 NLYY2122 pKa = 5.94 WHH2124 pKa = 7.07 KK2125 pKa = 10.47 PRR2127 pKa = 11.84 NTFNFEE2133 pKa = 3.79 RR2134 pKa = 11.84 ALKK2137 pKa = 9.8 NLNIACRR2144 pKa = 11.84 QRR2146 pKa = 11.84 GIGSVSMPPMQCGLDD2161 pKa = 3.22 AKK2163 pKa = 9.5 YY2164 pKa = 8.14 THH2166 pKa = 6.68 LKK2168 pKa = 9.66 IWDD2171 pKa = 3.88 LEE2173 pKa = 4.14 KK2174 pKa = 11.15 LLTKK2178 pKa = 10.5 HH2179 pKa = 5.6 LTDD2182 pKa = 3.6 VAWTIFAA2189 pKa = 4.91
Molecular weight: 250.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.183
IPC2_protein 6.198
IPC_protein 6.262
Toseland 6.504
ProMoST 6.678
Dawson 6.605
Bjellqvist 6.62
Wikipedia 6.62
Rodwell 6.605
Grimsley 6.62
Solomon 6.605
Lehninger 6.605
Nozaki 6.912
DTASelect 7.044
Thurlkill 7.073
EMBOSS 7.044
Sillero 7.0
Patrickios 4.24
IPC_peptide 6.62
IPC2_peptide 6.825
IPC2.peptide.svr19 6.799
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3059
870
2189
1529.5
173.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.192 ± 0.309
1.733 ± 0.345
5.165 ± 0.053
6.571 ± 0.862
5.263 ± 0.239
5.361 ± 0.134
2.517 ± 0.278
6.865 ± 0.553
5.721 ± 0.896
8.238 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.779 ± 0.351
5.623 ± 0.63
4.021 ± 0.512
4.119 ± 0.123
5.034 ± 0.557
6.048 ± 0.761
6.996 ± 1.144
6.015 ± 0.265
1.275 ± 0.289
3.465 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here