Pseudomonas phage Skulduggery
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0SZB0|A0A1Y0SZB0_9CAUD Uncharacterized protein OS=Pseudomonas phage Skulduggery OX=2006671 GN=SKUL_14 PE=4 SV=1
MM1 pKa = 7.54 SKK3 pKa = 9.97 QPQTLTVGGKK13 pKa = 9.22 EE14 pKa = 3.84 LVEE17 pKa = 4.62 GYY19 pKa = 10.87 GFAFVGNYY27 pKa = 9.95 FMDD30 pKa = 4.73 GKK32 pKa = 9.86 HH33 pKa = 5.24 GQIGVSGGFDD43 pKa = 3.33 EE44 pKa = 4.71 EE45 pKa = 4.65 TAHH48 pKa = 5.76 ITATISILTILGKK61 pKa = 10.6 AFDD64 pKa = 4.86 RR65 pKa = 11.84 NLQSGVPAQPLIEE78 pKa = 5.06 DD79 pKa = 3.83 PVFTLTIDD87 pKa = 3.92 GLPDD91 pKa = 4.0 FVSGRR96 pKa = 11.84 TEE98 pKa = 4.51 PYY100 pKa = 9.02 PLKK103 pKa = 10.69 AVYY106 pKa = 10.0 QLGADD111 pKa = 4.56 LAADD115 pKa = 4.31 DD116 pKa = 4.27 TPGAYY121 pKa = 9.98 GEE123 pKa = 5.0 LIVDD127 pKa = 3.69 AGYY130 pKa = 10.77 NYY132 pKa = 10.05 EE133 pKa = 3.9 RR134 pKa = 11.84 AEE136 pKa = 4.43 AIGAQRR142 pKa = 11.84 IVQQIAAEE150 pKa = 4.12 LAAQEE155 pKa = 4.32 EE156 pKa = 4.8
Molecular weight: 16.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.246
IPC2_protein 4.253
IPC_protein 4.164
Toseland 3.986
ProMoST 4.253
Dawson 4.126
Bjellqvist 4.329
Wikipedia 4.012
Rodwell 3.999
Grimsley 3.897
Solomon 4.113
Lehninger 4.075
Nozaki 4.24
DTASelect 4.406
Thurlkill 4.012
EMBOSS 4.024
Sillero 4.279
Patrickios 3.376
IPC_peptide 4.126
IPC2_peptide 4.266
IPC2.peptide.svr19 4.21
Protein with the highest isoelectric point:
>tr|A0A1Y0SZK2|A0A1Y0SZK2_9CAUD Uncharacterized protein OS=Pseudomonas phage Skulduggery OX=2006671 GN=SKUL_7 PE=4 SV=1
MM1 pKa = 7.14 VSAPGRR7 pKa = 11.84 RR8 pKa = 11.84 DD9 pKa = 4.2 DD10 pKa = 4.85 IMLKK14 pKa = 8.51 IHH16 pKa = 6.57 ARR18 pKa = 11.84 VEE20 pKa = 4.2 VVDD23 pKa = 3.79 MGFVLDD29 pKa = 5.24 GKK31 pKa = 9.82 PSPCHH36 pKa = 6.48 LWTGPHH42 pKa = 6.41 SGTGRR47 pKa = 11.84 GGGYY51 pKa = 10.84 GRR53 pKa = 11.84 MHH55 pKa = 7.62 LSGQTVAVHH64 pKa = 5.89 IVVFTHH70 pKa = 6.81 FYY72 pKa = 10.82 GYY74 pKa = 11.05 VPGKK78 pKa = 10.04 KK79 pKa = 9.86 QIDD82 pKa = 3.91 HH83 pKa = 6.47 KK84 pKa = 10.93 CSNRR88 pKa = 11.84 TCCNPAHH95 pKa = 6.27 LQMVTHH101 pKa = 6.77 KK102 pKa = 10.53 QNQRR106 pKa = 11.84 LRR108 pKa = 11.84 DD109 pKa = 3.5 KK110 pKa = 11.04 RR111 pKa = 11.84 AGAKK115 pKa = 9.54 KK116 pKa = 10.36 AA117 pKa = 3.38
Molecular weight: 12.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.219
IPC2_protein 9.355
IPC_protein 9.414
Toseland 10.248
ProMoST 9.823
Dawson 10.379
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.877
Grimsley 10.423
Solomon 10.423
Lehninger 10.394
Nozaki 10.292
DTASelect 9.999
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.306
Patrickios 10.628
IPC_peptide 10.423
IPC2_peptide 8.99
IPC2.peptide.svr19 8.25
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
20252
47
3695
281.3
30.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.382 ± 0.474
0.879 ± 0.164
6.197 ± 0.193
6.73 ± 0.284
3.373 ± 0.162
8.72 ± 0.368
1.778 ± 0.12
4.498 ± 0.185
5.555 ± 0.257
7.728 ± 0.25
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.79 ± 0.147
3.733 ± 0.121
4.8 ± 0.222
4.829 ± 0.217
5.792 ± 0.222
5.16 ± 0.181
6.059 ± 0.243
5.915 ± 0.267
1.358 ± 0.133
2.726 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here