Kineobactrum sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Kineobactrum

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N5XZW2|A0A2N5XZW2_9GAMM LLM class F420-dependent oxidoreductase OS=Kineobactrum sediminis OX=1905677 GN=CWI75_14620 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.12VQHH5 pKa = 5.87VAVVCVVGLLSCLSQAAHH23 pKa = 6.74AAAPVAVEE31 pKa = 4.14DD32 pKa = 3.59NYY34 pKa = 11.57GRR36 pKa = 11.84FFSGANVLDD45 pKa = 4.12NDD47 pKa = 4.51SDD49 pKa = 4.86ADD51 pKa = 4.36DD52 pKa = 5.49DD53 pKa = 4.6PLSADD58 pKa = 4.94LVSQPQGGTVFLQTNGVFEE77 pKa = 4.13YY78 pKa = 10.6TPDD81 pKa = 3.53EE82 pKa = 4.78GFSGPDD88 pKa = 2.99TFQYY92 pKa = 10.23RR93 pKa = 11.84AEE95 pKa = 4.16AAGEE99 pKa = 3.77FSNVVNVYY107 pKa = 9.75LQVGGSTSEE116 pKa = 4.37SNTPPVAVDD125 pKa = 3.52DD126 pKa = 4.83NYY128 pKa = 11.59GGDD131 pKa = 3.62YY132 pKa = 10.71LGQNVLSNDD141 pKa = 3.08SDD143 pKa = 4.64AEE145 pKa = 4.2GDD147 pKa = 3.78PLAADD152 pKa = 4.9LVSQPLGGTVFMQLDD167 pKa = 4.05GTFSYY172 pKa = 10.27TPNEE176 pKa = 4.21GFSGSDD182 pKa = 2.88SFQYY186 pKa = 10.38RR187 pKa = 11.84AVANGDD193 pKa = 3.38FSNIATVTLFVEE205 pKa = 5.91GPANSPPVAQDD216 pKa = 2.98DD217 pKa = 4.94SYY219 pKa = 12.14GVDD222 pKa = 3.44YY223 pKa = 10.94QGKK226 pKa = 8.74NVLANDD232 pKa = 3.65TDD234 pKa = 3.74IDD236 pKa = 4.37EE237 pKa = 5.46DD238 pKa = 4.31PLTAILVTQPEE249 pKa = 4.94LGTVTLNSDD258 pKa = 3.46GTFTYY263 pKa = 10.01TPNTGASGNDD273 pKa = 2.85VFTYY277 pKa = 9.81QASDD281 pKa = 3.46GTDD284 pKa = 3.24LSNVATVSLLVEE296 pKa = 4.73APANSAPVAQDD307 pKa = 3.37DD308 pKa = 4.35NFGFDD313 pKa = 3.43YY314 pKa = 10.71QGRR317 pKa = 11.84NVLANDD323 pKa = 3.59TDD325 pKa = 3.69VDD327 pKa = 4.34EE328 pKa = 6.03DD329 pKa = 4.42PLTAILVTPIQNGSLTLNSDD349 pKa = 3.37GSFSYY354 pKa = 10.57TPATGFSGTDD364 pKa = 2.83SFTYY368 pKa = 9.72QASDD372 pKa = 3.17GSEE375 pKa = 4.02LSNTATVTLQVEE387 pKa = 4.87APAEE391 pKa = 4.17EE392 pKa = 4.44PVPLNPVALDD402 pKa = 3.91DD403 pKa = 4.6NFGADD408 pKa = 3.43FAGRR412 pKa = 11.84NVLANDD418 pKa = 4.15EE419 pKa = 4.68DD420 pKa = 4.46PSGAGLVALLEE431 pKa = 4.08QGPANGTVQLNSSGTFTYY449 pKa = 10.26EE450 pKa = 3.54PNAGFVGEE458 pKa = 5.07DD459 pKa = 3.37VFSYY463 pKa = 10.19RR464 pKa = 11.84ASNGEE469 pKa = 4.13LLSDD473 pKa = 3.81VATVTLLVVDD483 pKa = 4.3ATVTPAVAVDD493 pKa = 4.83DD494 pKa = 4.33EE495 pKa = 4.91FEE497 pKa = 5.21GDD499 pKa = 3.48FSGANLITNDD509 pKa = 4.57LYY511 pKa = 11.61ANPQDD516 pKa = 3.55LAAIKK521 pKa = 9.55VTDD524 pKa = 4.13PGEE527 pKa = 4.3GSLEE531 pKa = 4.07INPDD535 pKa = 2.72GSFTYY540 pKa = 10.35RR541 pKa = 11.84PFAGFTGNDD550 pKa = 2.95SFTYY554 pKa = 10.38LVRR557 pKa = 11.84DD558 pKa = 3.6PDD560 pKa = 3.95GLEE563 pKa = 4.24SNVATVLLTVDD574 pKa = 4.02GNTAGEE580 pKa = 4.38GGLAAKK586 pKa = 9.95KK587 pKa = 10.37DD588 pKa = 3.66ADD590 pKa = 3.97GTSVLVDD597 pKa = 3.44ATPNEE602 pKa = 3.99LAVARR607 pKa = 11.84RR608 pKa = 11.84FDD610 pKa = 5.09DD611 pKa = 3.13ICTRR615 pKa = 11.84LDD617 pKa = 3.57PANADD622 pKa = 3.67QQDD625 pKa = 4.48LLTLCANLRR634 pKa = 11.84KK635 pKa = 9.92QGTTAKK641 pKa = 9.98QALDD645 pKa = 3.76ALRR648 pKa = 11.84VITPEE653 pKa = 3.49EE654 pKa = 3.58LAAIGKK660 pKa = 5.66TVRR663 pKa = 11.84VLSFSQFRR671 pKa = 11.84NHH673 pKa = 6.25GSRR676 pKa = 11.84MARR679 pKa = 11.84VRR681 pKa = 11.84EE682 pKa = 4.0GSRR685 pKa = 11.84GVSLAGLDD693 pKa = 3.8LNYY696 pKa = 10.62EE697 pKa = 4.18NSRR700 pKa = 11.84VSGSTLDD707 pKa = 4.2ALLQEE712 pKa = 4.53SLGMGASGDD721 pKa = 3.59EE722 pKa = 4.04MLAGSRR728 pKa = 11.84LGVFVTGDD736 pKa = 3.87LSFGDD741 pKa = 3.55QDD743 pKa = 3.53RR744 pKa = 11.84TGLEE748 pKa = 3.83SGFDD752 pKa = 3.73FDD754 pKa = 5.18SQTLTVGMDD763 pKa = 3.21YY764 pKa = 11.12RR765 pKa = 11.84LTDD768 pKa = 3.37NAFVGTSLSIGQAEE782 pKa = 4.44VTFDD786 pKa = 3.42QQQGEE791 pKa = 4.69TTTDD795 pKa = 3.15NQALAVYY802 pKa = 10.01GSLYY806 pKa = 10.42SGNGYY811 pKa = 10.19VDD813 pKa = 5.59GIFSYY818 pKa = 10.36GWSDD822 pKa = 2.94VDD824 pKa = 3.44TEE826 pKa = 4.7RR827 pKa = 11.84NISYY831 pKa = 10.5QDD833 pKa = 3.07FGGSVEE839 pKa = 4.09RR840 pKa = 11.84TAAGSTDD847 pKa = 2.83GSEE850 pKa = 4.73YY851 pKa = 10.38YY852 pKa = 11.0VSVNAGYY859 pKa = 10.31SFSSGALRR867 pKa = 11.84LDD869 pKa = 3.13PVMRR873 pKa = 11.84FFYY876 pKa = 10.9LDD878 pKa = 3.31GSVDD882 pKa = 3.48GFTEE886 pKa = 4.4TGAQGLNLAVSDD898 pKa = 4.75NDD900 pKa = 3.83FQSMTVSASGQLSYY914 pKa = 10.79MFLPGWGVITPYY926 pKa = 10.8LRR928 pKa = 11.84LEE930 pKa = 4.14YY931 pKa = 10.16TRR933 pKa = 11.84EE934 pKa = 4.17LEE936 pKa = 4.18DD937 pKa = 3.05SAAGVRR943 pKa = 11.84YY944 pKa = 9.87RR945 pKa = 11.84FVNDD949 pKa = 3.96PFADD953 pKa = 3.37STLQIRR959 pKa = 11.84VDD961 pKa = 4.5DD962 pKa = 5.04PDD964 pKa = 3.41TSYY967 pKa = 10.61MVYY970 pKa = 10.29GAGVAAQFAHH980 pKa = 6.6GFSGFVSYY988 pKa = 10.51QALGGYY994 pKa = 9.98DD995 pKa = 3.58GLSGEE1000 pKa = 4.59TVSFGARR1007 pKa = 11.84WEE1009 pKa = 4.08LAFF1012 pKa = 5.37

Molecular weight: 106.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N5Y544|A0A2N5Y544_9GAMM Type IV pili twitching motility protein PilT OS=Kineobactrum sediminis OX=1905677 GN=CWI75_03960 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.87RR14 pKa = 11.84THH16 pKa = 5.99GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.46NGRR28 pKa = 11.84LLLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3544

0

3544

1232078

27

3408

347.7

38.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.86 ± 0.046

1.029 ± 0.016

5.834 ± 0.035

6.164 ± 0.037

3.631 ± 0.027

8.171 ± 0.038

2.249 ± 0.022

5.05 ± 0.03

2.791 ± 0.032

10.861 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.019

3.086 ± 0.026

4.845 ± 0.026

4.025 ± 0.028

6.711 ± 0.033

5.774 ± 0.026

5.217 ± 0.025

7.222 ± 0.034

1.399 ± 0.017

2.698 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski