Faecalicatena orotica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Faecalicatena

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Y9C692|A0A2Y9C692_9FIRM Formate acetyltransferase OS=Faecalicatena orotica OX=1544 GN=A8806_11473 PE=3 SV=1
MM1 pKa = 6.83STHH4 pKa = 5.59RR5 pKa = 11.84HH6 pKa = 5.32IDD8 pKa = 3.69KK9 pKa = 10.43ICCAVLLAVFILTAVFCNAEE29 pKa = 4.01ALGVEE34 pKa = 4.73TVSSAFGYY42 pKa = 9.84EE43 pKa = 3.85GKK45 pKa = 10.71LFDD48 pKa = 3.98TSSVHH53 pKa = 6.74TIDD56 pKa = 5.05IVMDD60 pKa = 3.69DD61 pKa = 3.12WDD63 pKa = 4.99GFLEE67 pKa = 4.35TCTNEE72 pKa = 3.88EE73 pKa = 4.12YY74 pKa = 10.36TICAVVIDD82 pKa = 3.83NEE84 pKa = 4.25AYY86 pKa = 9.58KK87 pKa = 10.94NVGIRR92 pKa = 11.84AKK94 pKa = 10.87GNTSLSSVAAYY105 pKa = 10.51GNDD108 pKa = 3.09RR109 pKa = 11.84YY110 pKa = 11.01SFKK113 pKa = 10.68IEE115 pKa = 3.57FDD117 pKa = 3.72HH118 pKa = 8.26YY119 pKa = 11.7DD120 pKa = 3.35DD121 pKa = 4.16AKK123 pKa = 11.21NYY125 pKa = 10.3YY126 pKa = 10.06GLDD129 pKa = 3.71KK130 pKa = 10.87ISLNNIIQDD139 pKa = 3.55NTYY142 pKa = 9.28MKK144 pKa = 10.48DD145 pKa = 3.53YY146 pKa = 10.92LSYY149 pKa = 11.58QMMGYY154 pKa = 10.18FGVDD158 pKa = 3.15APLCSYY164 pKa = 11.84ANITVNGEE172 pKa = 3.64DD173 pKa = 3.02WGLYY177 pKa = 9.44LAVEE181 pKa = 4.5GVEE184 pKa = 4.32EE185 pKa = 4.52SFLTRR190 pKa = 11.84NYY192 pKa = 10.49GSDD195 pKa = 3.3YY196 pKa = 11.43GEE198 pKa = 4.5LYY200 pKa = 10.7KK201 pKa = 10.38PDD203 pKa = 4.67SISMGGGRR211 pKa = 11.84GNGGGFDD218 pKa = 3.35MEE220 pKa = 4.66DD221 pKa = 3.39FEE223 pKa = 5.2EE224 pKa = 5.48RR225 pKa = 11.84SGQDD229 pKa = 3.07GEE231 pKa = 4.91ADD233 pKa = 3.59TEE235 pKa = 4.13EE236 pKa = 5.48DD237 pKa = 3.52SGKK240 pKa = 9.02DD241 pKa = 3.31TSGKK245 pKa = 8.24ATPGGGMKK253 pKa = 10.18PGGRR257 pKa = 11.84GQPEE261 pKa = 5.12DD262 pKa = 3.53GTLPDD267 pKa = 4.46MDD269 pKa = 5.58DD270 pKa = 3.84MPDD273 pKa = 3.27TGTMPEE279 pKa = 4.29KK280 pKa = 10.75GAMPDD285 pKa = 3.32MGDD288 pKa = 3.52MPEE291 pKa = 4.93DD292 pKa = 3.27GAMPDD297 pKa = 3.71MGNMPGDD304 pKa = 3.28GAMPDD309 pKa = 3.58MGDD312 pKa = 3.42MPEE315 pKa = 5.39DD316 pKa = 3.42GTLPEE321 pKa = 4.54MGDD324 pKa = 3.52MPGNGAMPANGGGMGMGSDD343 pKa = 4.34DD344 pKa = 3.47VSLIYY349 pKa = 10.62SDD351 pKa = 5.75DD352 pKa = 4.26DD353 pKa = 3.43QDD355 pKa = 3.98SYY357 pKa = 12.41SNIFDD362 pKa = 3.67NAKK365 pKa = 9.91TNVSDD370 pKa = 3.81ADD372 pKa = 3.57KK373 pKa = 10.96EE374 pKa = 4.13RR375 pKa = 11.84LIEE378 pKa = 4.17SLKK381 pKa = 9.7TLNEE385 pKa = 4.23GGDD388 pKa = 3.46IEE390 pKa = 5.21SVVDD394 pKa = 3.33VDD396 pKa = 3.45EE397 pKa = 4.76VIRR400 pKa = 11.84YY401 pKa = 7.5FVVHH405 pKa = 6.34NFVCNFDD412 pKa = 4.16SYY414 pKa = 9.87TGSMIHH420 pKa = 6.56NYY422 pKa = 9.82YY423 pKa = 10.43LYY425 pKa = 10.83EE426 pKa = 4.36EE427 pKa = 5.03DD428 pKa = 5.07GVMSMIPWDD437 pKa = 3.65YY438 pKa = 11.35NLAFGGFMSGSDD450 pKa = 3.24AGSLVNYY457 pKa = 9.61PIDD460 pKa = 3.82TPVSGGTVEE469 pKa = 4.23SRR471 pKa = 11.84PMLAWIFNEE480 pKa = 3.84EE481 pKa = 4.58AYY483 pKa = 9.94TEE485 pKa = 4.33IYY487 pKa = 10.12HH488 pKa = 6.82EE489 pKa = 4.33YY490 pKa = 8.54FAEE493 pKa = 5.19FISEE497 pKa = 4.28YY498 pKa = 10.23FDD500 pKa = 3.05SGYY503 pKa = 10.0FEE505 pKa = 5.35EE506 pKa = 5.37MIDD509 pKa = 4.16SVSEE513 pKa = 3.87MIAPYY518 pKa = 10.51VEE520 pKa = 4.0NDD522 pKa = 3.19PTKK525 pKa = 9.44FCTYY529 pKa = 10.55EE530 pKa = 3.81EE531 pKa = 4.4FEE533 pKa = 4.7EE534 pKa = 5.21GVSTLKK540 pKa = 10.53EE541 pKa = 3.98FCLLRR546 pKa = 11.84AEE548 pKa = 4.93SINGQLDD555 pKa = 3.45GTIPSTSDD563 pKa = 3.04GQGEE567 pKa = 4.43DD568 pKa = 3.76DD569 pKa = 4.9SALIDD574 pKa = 3.93AGDD577 pKa = 3.86IEE579 pKa = 4.65VSAMGSMGNMMGMGGSKK596 pKa = 10.55NKK598 pKa = 9.72GRR600 pKa = 4.06

Molecular weight: 65.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Y9CAT9|A0A2Y9CAT9_9FIRM Protein N-acetyltransferase RimJ/RimL family OS=Faecalicatena orotica OX=1544 GN=A8806_12310 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.63NRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.89VHH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5138

0

5138

1715350

28

2958

333.9

37.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.391 ± 0.035

1.523 ± 0.014

5.585 ± 0.023

7.671 ± 0.037

4.197 ± 0.022

7.317 ± 0.029

1.753 ± 0.013

7.437 ± 0.033

6.656 ± 0.025

9.02 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.245 ± 0.019

4.19 ± 0.022

3.349 ± 0.02

3.218 ± 0.019

4.335 ± 0.022

5.761 ± 0.025

5.292 ± 0.025

6.878 ± 0.027

0.967 ± 0.012

4.215 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski