bacterium D16-50

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4338 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9D3L7|A0A3A9D3L7_9BACT Esterase family protein OS=bacterium D16-50 OX=2320112 GN=D7X48_18660 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 10.35RR3 pKa = 11.84KK4 pKa = 9.49LLSLVLSAALVLSMAACGNEE24 pKa = 3.92DD25 pKa = 3.52EE26 pKa = 5.04PANGSTPQDD35 pKa = 3.6SVSLSSEE42 pKa = 4.31PEE44 pKa = 3.84SSSAGEE50 pKa = 4.0PSDD53 pKa = 5.01EE54 pKa = 4.5GDD56 pKa = 3.71ASDD59 pKa = 4.44ASDD62 pKa = 3.29VGGAASGEE70 pKa = 4.01PTVIRR75 pKa = 11.84FGTHH79 pKa = 5.01WVPEE83 pKa = 4.26LDD85 pKa = 3.41PYY87 pKa = 9.96YY88 pKa = 9.75TDD90 pKa = 3.77EE91 pKa = 4.28VTGEE95 pKa = 3.87YY96 pKa = 10.77TMGEE100 pKa = 4.36RR101 pKa = 11.84EE102 pKa = 4.04RR103 pKa = 11.84QAALAGLEE111 pKa = 4.42AIKK114 pKa = 10.4KK115 pKa = 8.77AYY117 pKa = 9.68NVEE120 pKa = 4.5FEE122 pKa = 4.11FLQYY126 pKa = 10.44PVDD129 pKa = 3.85VASDD133 pKa = 3.6LMTSVLAQDD142 pKa = 5.27PICDD146 pKa = 3.99LALMWGGVEE155 pKa = 4.02PTILAQNVLQDD166 pKa = 3.4LSAYY170 pKa = 9.0TGLFEE175 pKa = 6.52DD176 pKa = 6.13DD177 pKa = 3.41EE178 pKa = 5.21ASWLLKK184 pKa = 10.6GSVFDD189 pKa = 4.62GYY191 pKa = 11.73YY192 pKa = 10.37LLGYY196 pKa = 9.87EE197 pKa = 4.5FGEE200 pKa = 4.44TSFPLIVNMTMLEE213 pKa = 4.15AVDD216 pKa = 3.89SLKK219 pKa = 11.19DD220 pKa = 3.73EE221 pKa = 4.5NGKK224 pKa = 8.31TIYY227 pKa = 8.85PTDD230 pKa = 3.64LFKK233 pKa = 10.96EE234 pKa = 5.01GKK236 pKa = 6.35WTWSTFKK243 pKa = 10.93DD244 pKa = 3.51YY245 pKa = 10.85LAKK248 pKa = 10.43IKK250 pKa = 10.41AYY252 pKa = 8.9YY253 pKa = 10.4SNVAAPDD260 pKa = 3.54GAYY263 pKa = 10.4YY264 pKa = 10.51DD265 pKa = 3.95YY266 pKa = 11.27VQAYY270 pKa = 6.54EE271 pKa = 3.84TDD273 pKa = 3.51FRR275 pKa = 11.84YY276 pKa = 9.89AALGAVHH283 pKa = 7.03ANGGAIYY290 pKa = 10.27DD291 pKa = 3.97GGVTADD297 pKa = 3.24SAEE300 pKa = 4.26TIEE303 pKa = 4.09AVAYY307 pKa = 9.43IQEE310 pKa = 4.36LVDD313 pKa = 4.19EE314 pKa = 4.78EE315 pKa = 5.41LLTACHH321 pKa = 6.48LQDD324 pKa = 5.37DD325 pKa = 4.82YY326 pKa = 11.68TPEE329 pKa = 3.97WLRR332 pKa = 11.84GGNDD336 pKa = 3.17FGKK339 pKa = 10.49GATVFTDD346 pKa = 3.22CGGWMIGGHH355 pKa = 4.91STEE358 pKa = 4.28CAARR362 pKa = 11.84GEE364 pKa = 4.64SIGIIPWPMPDD375 pKa = 3.84DD376 pKa = 3.61ATVDD380 pKa = 3.63SEE382 pKa = 4.92TYY384 pKa = 9.8RR385 pKa = 11.84QSTNGGNSVGVLKK398 pKa = 10.75GVSPEE403 pKa = 3.98KK404 pKa = 10.48TEE406 pKa = 4.14LALKK410 pKa = 10.75AFILYY415 pKa = 7.24WQTYY419 pKa = 7.91HH420 pKa = 6.07KK421 pKa = 9.31TIAGVDD427 pKa = 4.05TIADD431 pKa = 3.56YY432 pKa = 10.62HH433 pKa = 6.63AAAALDD439 pKa = 3.6KK440 pKa = 10.84LAGYY444 pKa = 10.1GVDD447 pKa = 5.41LYY449 pKa = 11.58NEE451 pKa = 4.52TYY453 pKa = 11.03GDD455 pKa = 4.44DD456 pKa = 5.93LIDD459 pKa = 4.4CYY461 pKa = 10.63TYY463 pKa = 11.03IMSHH467 pKa = 6.09MSTNYY472 pKa = 10.49ANMMGLWEE480 pKa = 5.64DD481 pKa = 4.56YY482 pKa = 8.13NTHH485 pKa = 5.96DD486 pKa = 3.4QWTEE490 pKa = 3.4ILGQSLFKK498 pKa = 10.98APGMSSYY505 pKa = 11.43DD506 pKa = 3.45VAIKK510 pKa = 10.92ANLTNLTNKK519 pKa = 8.73TDD521 pKa = 3.77SIAAVLKK528 pKa = 10.32TEE530 pKa = 4.5GVHH533 pKa = 7.47DD534 pKa = 4.0NQKK537 pKa = 10.95PNITKK542 pKa = 10.24EE543 pKa = 4.04KK544 pKa = 10.59AILTAGAADD553 pKa = 3.99VDD555 pKa = 3.6WTQYY559 pKa = 10.45FSAEE563 pKa = 4.1DD564 pKa = 3.5AVDD567 pKa = 3.92GVIEE571 pKa = 4.1ITADD575 pKa = 3.44SITVNEE581 pKa = 4.35GLDD584 pKa = 3.59LNTPGKK590 pKa = 9.76YY591 pKa = 9.42EE592 pKa = 3.89KK593 pKa = 10.77AVTAKK598 pKa = 10.85ASDD601 pKa = 3.31KK602 pKa = 11.09SGNEE606 pKa = 3.65ASSDD610 pKa = 3.53LTVIVYY616 pKa = 10.1DD617 pKa = 4.89ADD619 pKa = 3.66NTDD622 pKa = 3.73APTAAAKK629 pKa = 10.63EE630 pKa = 4.01EE631 pKa = 4.4LPTVALNTEE640 pKa = 3.99TSGINWSDD648 pKa = 3.52FLEE651 pKa = 4.5SAEE654 pKa = 4.59DD655 pKa = 3.83ADD657 pKa = 4.94GVDD660 pKa = 3.41VKK662 pKa = 11.72DD663 pKa = 4.27NVTADD668 pKa = 4.06LSTLDD673 pKa = 3.31TTTPGEE679 pKa = 4.15YY680 pKa = 10.27DD681 pKa = 3.37VTLTVTDD688 pKa = 3.93YY689 pKa = 11.45VGNTAEE695 pKa = 4.13ITVKK699 pKa = 9.43VTVVSEE705 pKa = 4.05

Molecular weight: 76.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9CZ26|A0A3A9CZ26_9BACT Uncharacterized protein OS=bacterium D16-50 OX=2320112 GN=D7X48_20390 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.58QPKK8 pKa = 9.19KK9 pKa = 8.22RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 8.61VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.98GRR39 pKa = 11.84KK40 pKa = 8.68ILSAA44 pKa = 4.02

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4338

0

4338

1406950

25

3304

324.3

36.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.936 ± 0.04

1.589 ± 0.015

5.505 ± 0.028

8.003 ± 0.046

4.037 ± 0.026

7.831 ± 0.044

1.701 ± 0.017

6.303 ± 0.04

5.991 ± 0.04

9.29 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.081 ± 0.017

3.857 ± 0.026

3.401 ± 0.024

3.421 ± 0.024

5.553 ± 0.038

5.749 ± 0.034

4.829 ± 0.035

6.629 ± 0.032

1.076 ± 0.016

4.217 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski