Gammapapillomavirus 7
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2ALC5|A0A2D2ALC5_9PAPI Regulatory protein E2 OS=Gammapapillomavirus 7 OX=1175849 GN=E2 PE=3 SV=1
MM1 pKa = 8.1 DD2 pKa = 3.86 SEE4 pKa = 4.64 RR5 pKa = 11.84 PRR7 pKa = 11.84 KK8 pKa = 8.02 RR9 pKa = 11.84 TKK11 pKa = 10.15 RR12 pKa = 11.84 DD13 pKa = 3.08 SVTNLYY19 pKa = 7.64 RR20 pKa = 11.84 TCQISGNCPEE30 pKa = 4.16 DD31 pKa = 3.32 VKK33 pKa = 11.62 NKK35 pKa = 10.04 VEE37 pKa = 4.34 ANTLADD43 pKa = 3.34 KK44 pKa = 10.86 LLRR47 pKa = 11.84 ILSSIVYY54 pKa = 9.96 FGGLGIGTGKK64 pKa = 8.78 GTGGSTGYY72 pKa = 10.49 RR73 pKa = 11.84 PISSGGGGGGRR84 pKa = 11.84 VTGDD88 pKa = 3.05 GTVIRR93 pKa = 11.84 PNIVVEE99 pKa = 4.07 PVGPSDD105 pKa = 5.19 VIPIDD110 pKa = 3.61 ALSPGSSSIVPLQEE124 pKa = 3.95 AGAEE128 pKa = 4.07 IIIPDD133 pKa = 3.52 ISTDD137 pKa = 3.39 LGAGEE142 pKa = 5.07 LEE144 pKa = 4.97 VITEE148 pKa = 4.14 PDD150 pKa = 3.51 IIDD153 pKa = 3.05 ISGPQEE159 pKa = 4.21 SPTVTTSEE167 pKa = 4.87 DD168 pKa = 2.89 IAIIDD173 pKa = 3.97 VQPGPSTPRR182 pKa = 11.84 RR183 pKa = 11.84 VVTSSSRR190 pKa = 11.84 FSNPSFISVVTSSSTTEE207 pKa = 3.99 AGSTAIDD214 pKa = 3.87 VFVHH218 pKa = 6.25 GAAGGDD224 pKa = 3.56 IIGEE228 pKa = 4.38 EE229 pKa = 4.06 IPLDD233 pKa = 3.58 TFNEE237 pKa = 4.12 PEE239 pKa = 3.97 EE240 pKa = 4.42 FEE242 pKa = 5.27 IEE244 pKa = 5.33 DD245 pKa = 3.54 IPQPRR250 pKa = 11.84 SSTPRR255 pKa = 11.84 AFQRR259 pKa = 11.84 AFSRR263 pKa = 11.84 ARR265 pKa = 11.84 DD266 pKa = 3.56 LYY268 pKa = 9.85 NRR270 pKa = 11.84 RR271 pKa = 11.84 VRR273 pKa = 11.84 QVMTQNINFLTRR285 pKa = 11.84 APQAVQFAFEE295 pKa = 4.96 NPAFDD300 pKa = 3.77 NDD302 pKa = 3.21 ITIEE306 pKa = 4.05 FQQDD310 pKa = 3.35 LNQLAAAAPDD320 pKa = 4.13 PDD322 pKa = 3.6 FADD325 pKa = 3.05 IVRR328 pKa = 11.84 LHH330 pKa = 6.86 RR331 pKa = 11.84 PRR333 pKa = 11.84 FSEE336 pKa = 3.96 TAEE339 pKa = 3.84 GQIRR343 pKa = 11.84 VSRR346 pKa = 11.84 LGTKK350 pKa = 8.53 GTIRR354 pKa = 11.84 LRR356 pKa = 11.84 SGTQIGEE363 pKa = 4.49 TVHH366 pKa = 7.0 FYY368 pKa = 11.54 YY369 pKa = 10.63 DD370 pKa = 3.62 LSSIEE375 pKa = 4.0 DD376 pKa = 3.42 AEE378 pKa = 4.58 AIEE381 pKa = 4.44 LSVLGEE387 pKa = 4.03 HH388 pKa = 6.72 SGDD391 pKa = 3.1 ATVINPQAEE400 pKa = 4.59 STFVDD405 pKa = 4.38 AEE407 pKa = 4.12 NSDD410 pKa = 3.86 APLLFPEE417 pKa = 5.06 EE418 pKa = 4.34 EE419 pKa = 4.16 LLDD422 pKa = 5.32 DD423 pKa = 4.48 ISEE426 pKa = 4.58 DD427 pKa = 3.66 FSNSHH432 pKa = 6.94 VILSSGGRR440 pKa = 11.84 RR441 pKa = 11.84 SVLSVPTLPPGVALKK456 pKa = 10.81 VFIDD460 pKa = 4.05 DD461 pKa = 3.45 VGEE464 pKa = 4.1 GLFVSHH470 pKa = 7.11 PVSTDD475 pKa = 3.34 SIPPGVIPISDD486 pKa = 3.86 IQPSILIDD494 pKa = 3.59 EE495 pKa = 4.96 FSSDD499 pKa = 4.99 DD500 pKa = 4.21 FILHH504 pKa = 7.02 PSHH507 pKa = 6.25 IPKK510 pKa = 10.32 RR511 pKa = 11.84 KK512 pKa = 8.36 RR513 pKa = 11.84 KK514 pKa = 9.46 RR515 pKa = 11.84 VTSLL519 pKa = 2.88
Molecular weight: 55.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.669
IPC2_protein 4.533
IPC_protein 4.507
Toseland 4.342
ProMoST 4.622
Dawson 4.457
Bjellqvist 4.609
Wikipedia 4.342
Rodwell 4.342
Grimsley 4.253
Solomon 4.457
Lehninger 4.418
Nozaki 4.558
DTASelect 4.749
Thurlkill 4.342
EMBOSS 4.355
Sillero 4.622
Patrickios 4.177
IPC_peptide 4.457
IPC2_peptide 4.609
IPC2.peptide.svr19 4.561
Protein with the highest isoelectric point:
>tr|A0A2D2ALC6|A0A2D2ALC6_9PAPI ^E4 (Fragment) OS=Gammapapillomavirus 7 OX=1175849 GN=^E4 PE=4 SV=1
MM1 pKa = 7.57 EE2 pKa = 4.83 SLAEE6 pKa = 4.43 RR7 pKa = 11.84 FDD9 pKa = 4.13 ALQEE13 pKa = 4.18 NQLTLYY19 pKa = 7.69 EE20 pKa = 4.3 TGSTNIEE27 pKa = 3.92 DD28 pKa = 4.48 QILYY32 pKa = 9.33 WDD34 pKa = 4.05 TVRR37 pKa = 11.84 QEE39 pKa = 4.14 YY40 pKa = 11.14 VLFHH44 pKa = 5.95 YY45 pKa = 10.3 ARR47 pKa = 11.84 QRR49 pKa = 11.84 GLSHH53 pKa = 7.28 IGLQPVPTLTVSEE66 pKa = 4.51 QKK68 pKa = 10.75 AKK70 pKa = 10.47 QAILMVLQLKK80 pKa = 8.89 SLKK83 pKa = 9.98 QSQFGNEE90 pKa = 3.95 PWTMQDD96 pKa = 2.44 TSYY99 pKa = 11.41 EE100 pKa = 4.2 LFNTAPSNTFKK111 pKa = 10.85 KK112 pKa = 10.15 GAYY115 pKa = 8.18 TVDD118 pKa = 3.05 VYY120 pKa = 11.9 YY121 pKa = 11.01 DD122 pKa = 3.8 GDD124 pKa = 3.6 EE125 pKa = 4.67 DD126 pKa = 5.07 NYY128 pKa = 10.74 FPYY131 pKa = 10.0 TAWSYY136 pKa = 11.28 IYY138 pKa = 9.86 YY139 pKa = 10.45 QNGDD143 pKa = 3.96 NEE145 pKa = 4.16 WHH147 pKa = 6.37 KK148 pKa = 11.48 VKK150 pKa = 10.95 GDD152 pKa = 3.1 IDD154 pKa = 4.34 YY155 pKa = 11.13 EE156 pKa = 4.35 GLFYY160 pKa = 10.77 KK161 pKa = 10.49 SHH163 pKa = 7.33 DD164 pKa = 3.84 GEE166 pKa = 4.05 KK167 pKa = 9.84 VYY169 pKa = 11.25 YY170 pKa = 8.49 VTFYY174 pKa = 11.59 DD175 pKa = 3.64 DD176 pKa = 3.19 ATRR179 pKa = 11.84 FSRR182 pKa = 11.84 TGKK185 pKa = 6.65 WTVRR189 pKa = 11.84 YY190 pKa = 9.85 KK191 pKa = 10.16 NTTISSTSITSTSGPTGNPTSKK213 pKa = 10.23 PSSDD217 pKa = 3.45 SVGQKK222 pKa = 8.88 ATEE225 pKa = 3.89 QASKK229 pKa = 10.49 RR230 pKa = 11.84 RR231 pKa = 11.84 QRR233 pKa = 11.84 SPSPSSPRR241 pKa = 11.84 HH242 pKa = 4.34 TRR244 pKa = 11.84 SRR246 pKa = 11.84 TWQSDD251 pKa = 2.78 GDD253 pKa = 4.18 TSSSSPGISTRR264 pKa = 11.84 RR265 pKa = 11.84 RR266 pKa = 11.84 RR267 pKa = 11.84 RR268 pKa = 11.84 EE269 pKa = 3.98 GKK271 pKa = 8.32 STTQRR276 pKa = 11.84 RR277 pKa = 11.84 EE278 pKa = 3.75 RR279 pKa = 11.84 PSRR282 pKa = 11.84 ATSPGVAPEE291 pKa = 4.0 EE292 pKa = 3.93 VGRR295 pKa = 11.84 RR296 pKa = 11.84 HH297 pKa = 6.98 RR298 pKa = 11.84 FTQRR302 pKa = 11.84 KK303 pKa = 7.77 GLSRR307 pKa = 11.84 LGQLQEE313 pKa = 4.32 DD314 pKa = 4.14 ARR316 pKa = 11.84 DD317 pKa = 3.63 PPIILLKK324 pKa = 10.96 GPANTLKK331 pKa = 10.57 CYY333 pKa = 10.45 RR334 pKa = 11.84 FRR336 pKa = 11.84 CRR338 pKa = 11.84 SKK340 pKa = 10.62 CRR342 pKa = 11.84 SLIYY346 pKa = 10.52 RR347 pKa = 11.84 MSTVFSWVGEE357 pKa = 4.22 GPEE360 pKa = 3.94 RR361 pKa = 11.84 LGEE364 pKa = 4.03 PRR366 pKa = 11.84 MLLAFKK372 pKa = 10.59 DD373 pKa = 3.39 KK374 pKa = 10.34 EE375 pKa = 3.78 QRR377 pKa = 11.84 QRR379 pKa = 11.84 FLSTVHH385 pKa = 6.13 LPKK388 pKa = 9.91 GTHH391 pKa = 5.62 YY392 pKa = 11.42 SLGNLNSLL400 pKa = 4.21
Molecular weight: 46.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.865
IPC2_protein 9.151
IPC_protein 9.165
Toseland 9.56
ProMoST 9.443
Dawson 9.882
Bjellqvist 9.633
Wikipedia 10.101
Rodwell 10.072
Grimsley 9.984
Solomon 9.911
Lehninger 9.853
Nozaki 9.604
DTASelect 9.619
Thurlkill 9.692
EMBOSS 10.014
Sillero 9.809
Patrickios 4.851
IPC_peptide 9.897
IPC2_peptide 8.317
IPC2.peptide.svr19 8.027
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2413
93
617
344.7
38.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.263 ± 0.438
2.196 ± 0.788
6.672 ± 0.469
5.802 ± 0.783
4.31 ± 0.443
6.009 ± 0.792
1.782 ± 0.117
5.636 ± 0.945
5.222 ± 0.792
8.91 ± 0.848
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.575 ± 0.374
4.642 ± 0.729
6.051 ± 0.964
4.434 ± 0.371
5.802 ± 1.05
8.869 ± 0.792
6.299 ± 0.695
6.216 ± 0.573
1.202 ± 0.304
3.108 ± 0.634
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here