Streptococcus phage Javan464

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B533|A0A4D6B533_9CAUD Uncharacterized protein OS=Streptococcus phage Javan464 OX=2548185 GN=Javan464_0046 PE=4 SV=1
MM1 pKa = 7.43EE2 pKa = 5.08FVDD5 pKa = 4.87KK6 pKa = 10.36KK7 pKa = 10.84LSEE10 pKa = 3.74ITPYY14 pKa = 11.02KK15 pKa = 10.45NNPRR19 pKa = 11.84NNDD22 pKa = 3.24EE23 pKa = 4.0AVEE26 pKa = 4.0PVAEE30 pKa = 4.39SIKK33 pKa = 10.63EE34 pKa = 3.9FGFKK38 pKa = 10.7VPIVVDD44 pKa = 3.72KK45 pKa = 11.38NGEE48 pKa = 3.99IVNGHH53 pKa = 5.21TRR55 pKa = 11.84YY56 pKa = 9.98KK57 pKa = 10.46AAQKK61 pKa = 10.59LGLEE65 pKa = 4.26TVPVIVADD73 pKa = 4.4DD74 pKa = 3.97LSEE77 pKa = 4.06EE78 pKa = 4.08QIKK81 pKa = 10.65AFRR84 pKa = 11.84LADD87 pKa = 3.56NKK89 pKa = 10.32VSEE92 pKa = 4.23IAVWDD97 pKa = 4.02LDD99 pKa = 4.36LLNEE103 pKa = 4.26EE104 pKa = 5.17LNDD107 pKa = 4.3ILDD110 pKa = 4.08LDD112 pKa = 3.72MSVFGFEE119 pKa = 5.75LEE121 pKa = 4.35IDD123 pKa = 4.55DD124 pKa = 5.0EE125 pKa = 4.41NQEE128 pKa = 4.06NLDD131 pKa = 3.56ADD133 pKa = 3.88FEE135 pKa = 4.69EE136 pKa = 6.03IEE138 pKa = 4.73DD139 pKa = 3.95DD140 pKa = 3.71SVLIVEE146 pKa = 4.79AEE148 pKa = 4.12SEE150 pKa = 4.27EE151 pKa = 4.18EE152 pKa = 4.32LEE154 pKa = 4.0KK155 pKa = 11.04LYY157 pKa = 11.23DD158 pKa = 3.62EE159 pKa = 4.51FVEE162 pKa = 5.0RR163 pKa = 11.84GFKK166 pKa = 10.58CRR168 pKa = 11.84VSILL172 pKa = 3.46

Molecular weight: 19.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B692|A0A4D6B692_9CAUD Uncharacterized protein OS=Streptococcus phage Javan464 OX=2548185 GN=Javan464_0044 PE=4 SV=1
MM1 pKa = 7.5VILPDD6 pKa = 3.68YY7 pKa = 10.67QGVGLGTRR15 pKa = 11.84FLKK18 pKa = 10.74SVAEE22 pKa = 4.4IYY24 pKa = 10.38SCQGFDD30 pKa = 3.73FRR32 pKa = 11.84IVTSAKK38 pKa = 10.61NLINALNRR46 pKa = 11.84STNWKK51 pKa = 9.6LKK53 pKa = 10.65SYY55 pKa = 11.09DD56 pKa = 3.33KK57 pKa = 11.45GKK59 pKa = 8.49TPTGNSSIKK68 pKa = 10.27QLAKK72 pKa = 9.42TARR75 pKa = 11.84KK76 pKa = 8.56NVKK79 pKa = 8.85IASFLFVRR87 pKa = 11.84KK88 pKa = 9.6DD89 pKa = 3.02

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12471

43

915

186.1

21.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.88 ± 0.52

0.666 ± 0.098

6.808 ± 0.297

7.537 ± 0.431

4.13 ± 0.208

5.789 ± 0.364

1.459 ± 0.153

7.273 ± 0.279

9.029 ± 0.394

8.339 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.153

5.573 ± 0.24

2.606 ± 0.175

4.001 ± 0.235

4.33 ± 0.227

5.982 ± 0.284

6.078 ± 0.268

6.142 ± 0.214

1.243 ± 0.157

3.705 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski