Bizionia paragorgiae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Bizionia

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2859 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3YW71|A0A1H3YW71_9FLAO Cystathionine gamma-lyase OS=Bizionia paragorgiae OX=283786 GN=SAMN04487990_107113 PE=3 SV=1
MM1 pKa = 7.17KK2 pKa = 9.86TNKK5 pKa = 9.65FLTLILSFVAVFAMTSCVEE24 pKa = 4.48DD25 pKa = 4.45DD26 pKa = 4.72DD27 pKa = 4.46FTVPSSLGNEE37 pKa = 3.78EE38 pKa = 3.65NAALVEE44 pKa = 4.0LLEE47 pKa = 4.56TSTEE51 pKa = 3.35VDD53 pKa = 2.95MAYY56 pKa = 10.57VQGLYY61 pKa = 10.25TSGTAPEE68 pKa = 4.44QIAADD73 pKa = 4.05IYY75 pKa = 9.63VKK77 pKa = 10.72GYY79 pKa = 10.41VSSSDD84 pKa = 3.2ATGNFYY90 pKa = 11.0KK91 pKa = 10.25EE92 pKa = 5.23FYY94 pKa = 9.55MQDD97 pKa = 3.27TPSNPTKK104 pKa = 10.19ALKK107 pKa = 10.34IIVSQSDD114 pKa = 3.89TYY116 pKa = 11.53NQFNKK121 pKa = 9.74GRR123 pKa = 11.84EE124 pKa = 3.93VYY126 pKa = 10.44INLKK130 pKa = 9.33GLYY133 pKa = 9.51VGEE136 pKa = 4.03EE137 pKa = 3.74RR138 pKa = 11.84VGNGVYY144 pKa = 10.21TIGGSVEE151 pKa = 3.77TDD153 pKa = 3.01QFGTTVQQLDD163 pKa = 3.48EE164 pKa = 4.72HH165 pKa = 6.08QVEE168 pKa = 4.66TKK170 pKa = 9.9ILRR173 pKa = 11.84SGTTADD179 pKa = 4.6MIPLPKK185 pKa = 9.85TFSSINGSNVGMLVSVDD202 pKa = 3.39NVEE205 pKa = 4.66FADD208 pKa = 4.12DD209 pKa = 4.12LAGKK213 pKa = 10.28AYY215 pKa = 10.31FDD217 pKa = 4.6PNEE220 pKa = 4.35SYY222 pKa = 9.17DD223 pKa = 3.76TQRR226 pKa = 11.84TMQACEE232 pKa = 3.86GLSYY236 pKa = 10.6ATFQLEE242 pKa = 4.35TSSFASFKK250 pKa = 10.77QEE252 pKa = 3.77PLPIKK257 pKa = 10.41NGTISAVVVKK267 pKa = 9.62TFDD270 pKa = 3.22GSQVVMALNSINDD283 pKa = 3.45VNFTDD288 pKa = 4.74ARR290 pKa = 11.84CSLLDD295 pKa = 3.44PTDD298 pKa = 3.61FTEE301 pKa = 4.77IFSEE305 pKa = 4.64DD306 pKa = 4.24FEE308 pKa = 4.76TMAANQSINANGWTNYY324 pKa = 9.47IEE326 pKa = 4.55AGNRR330 pKa = 11.84DD331 pKa = 3.12WRR333 pKa = 11.84VVVTTDD339 pKa = 2.86SGNPGSQVASFGAYY353 pKa = 10.09NSGDD357 pKa = 3.51VQNISWLITPGVDD370 pKa = 4.72LDD372 pKa = 3.84ASDD375 pKa = 4.84YY376 pKa = 11.13EE377 pKa = 4.32FLSFEE382 pKa = 4.34TSNSFSDD389 pKa = 4.03GSEE392 pKa = 3.93LEE394 pKa = 4.94LYY396 pKa = 10.28ISTDD400 pKa = 3.1WNGVEE405 pKa = 4.77ADD407 pKa = 3.75VANATWMPLSGTIVSDD423 pKa = 3.35GTFYY427 pKa = 11.06QDD429 pKa = 3.52WISSGSIDD437 pKa = 3.55LSSYY441 pKa = 10.98SGTAHH446 pKa = 6.61VAFKK450 pKa = 11.1YY451 pKa = 10.73VGGGDD456 pKa = 3.34AGIDD460 pKa = 3.43GTYY463 pKa = 10.72EE464 pKa = 4.04LDD466 pKa = 3.62NVSIVANN473 pKa = 3.97

Molecular weight: 51.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3YA99|A0A1H3YA99_9FLAO Glyceraldehyde 3-phosphate dehydrogenase OS=Bizionia paragorgiae OX=283786 GN=SAMN04487990_106115 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.92GRR40 pKa = 11.84KK41 pKa = 8.34KK42 pKa = 10.21ISVSSEE48 pKa = 3.74TRR50 pKa = 11.84HH51 pKa = 5.96KK52 pKa = 10.68KK53 pKa = 9.8

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2859

0

2859

932437

24

4616

326.1

36.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.59 ± 0.043

0.764 ± 0.016

5.644 ± 0.04

6.499 ± 0.047

5.139 ± 0.042

6.141 ± 0.044

1.867 ± 0.025

7.789 ± 0.044

7.775 ± 0.06

9.496 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.08 ± 0.021

6.064 ± 0.046

3.315 ± 0.026

3.414 ± 0.03

3.317 ± 0.031

6.538 ± 0.039

6.212 ± 0.062

6.38 ± 0.038

0.922 ± 0.014

4.053 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski