Erwinia phage EtG

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Eganvirus; Erwinia virus EtG

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218M4L9|A0A218M4L9_9CAUD Capsid scaffolding protein OS=Erwinia phage EtG OX=2014586 GN=EtG_04 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.1VYY4 pKa = 11.0AMQGDD9 pKa = 4.09TLDD12 pKa = 3.95ALCARR17 pKa = 11.84DD18 pKa = 3.52YY19 pKa = 11.51GRR21 pKa = 11.84TEE23 pKa = 4.09GVVEE27 pKa = 4.42TVLQANPGLSEE38 pKa = 4.6LGVILPHH45 pKa = 6.21GTAIDD50 pKa = 4.04LPDD53 pKa = 3.72VEE55 pKa = 5.11TSATAEE61 pKa = 4.16TLNLWDD67 pKa = 3.73

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218M4I2|A0A218M4I2_9CAUD Tail protein OS=Erwinia phage EtG OX=2014586 GN=EtG_30 PE=4 SV=1
MM1 pKa = 7.6LKK3 pKa = 10.14NEE5 pKa = 4.34PSFASLLVKK14 pKa = 10.25QSPGMHH20 pKa = 6.48YY21 pKa = 10.31GHH23 pKa = 6.73GWIAGKK29 pKa = 9.96DD30 pKa = 3.62GKK32 pKa = 10.06RR33 pKa = 11.84WHH35 pKa = 7.19PSRR38 pKa = 11.84SQADD42 pKa = 3.86LLAGLSTQRR51 pKa = 11.84QGEE54 pKa = 4.32SWLSKK59 pKa = 10.42LFRR62 pKa = 11.84LQYY65 pKa = 10.39RR66 pKa = 3.79

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

9688

44

791

215.3

23.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.363 ± 0.433

1.125 ± 0.154

6.08 ± 0.262

6.162 ± 0.281

3.386 ± 0.29

6.647 ± 0.32

1.765 ± 0.189

5.45 ± 0.293

5.46 ± 0.331

9.259 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.167

3.891 ± 0.197

4.263 ± 0.252

3.799 ± 0.27

6.245 ± 0.385

6.255 ± 0.225

6.575 ± 0.308

6.596 ± 0.341

1.569 ± 0.162

2.663 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski