Salmonella phage SEN8
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S6KZY9|A0A1S6KZY9_9CAUD Endolysin OS=Salmonella phage SEN8 OX=1647468 GN=SEN8_11 PE=3 SV=1
MM1 pKa = 7.72 KK2 pKa = 10.2 KK3 pKa = 10.08 YY4 pKa = 10.75 QDD6 pKa = 3.13 IKK8 pKa = 11.08 NFRR11 pKa = 11.84 LIDD14 pKa = 3.73 APVNRR19 pKa = 11.84 GKK21 pKa = 7.98 TQSEE25 pKa = 4.38 INIGAYY31 pKa = 9.83 FLEE34 pKa = 5.32 SEE36 pKa = 5.3 DD37 pKa = 3.64 GQDD40 pKa = 2.89 WYY42 pKa = 10.7 EE43 pKa = 4.14 CQSLFSDD50 pKa = 3.76 DD51 pKa = 3.73 TAKK54 pKa = 10.43 IMYY57 pKa = 9.57 DD58 pKa = 3.26 HH59 pKa = 7.22 EE60 pKa = 4.48 GVIWGVINKK69 pKa = 7.69 PVPQRR74 pKa = 11.84 GNTYY78 pKa = 9.8 AVSMLWPVNMSVAEE92 pKa = 4.35 IDD94 pKa = 5.01 AADD97 pKa = 4.62 CPDD100 pKa = 3.66 DD101 pKa = 4.29 CRR103 pKa = 11.84 GDD105 pKa = 4.09 GSWLYY110 pKa = 11.01 RR111 pKa = 11.84 DD112 pKa = 4.12 GKK114 pKa = 9.77 VLPVPVDD121 pKa = 3.49 YY122 pKa = 10.47 QAKK125 pKa = 10.73 AEE127 pKa = 4.19 TTRR130 pKa = 11.84 QKK132 pKa = 11.31 LLDD135 pKa = 3.97 AANSVIADD143 pKa = 3.37 WRR145 pKa = 11.84 TEE147 pKa = 3.73 LALGEE152 pKa = 4.44 ISDD155 pKa = 4.67 DD156 pKa = 4.32 DD157 pKa = 4.18 KK158 pKa = 12.09 ASLTKK163 pKa = 8.87 WMAYY167 pKa = 8.98 IRR169 pKa = 11.84 EE170 pKa = 4.38 LKK172 pKa = 10.76 SLVLTGISDD181 pKa = 3.44 EE182 pKa = 4.2 ATFNKK187 pKa = 9.4 IQWPVLPQQ195 pKa = 3.32
Molecular weight: 22.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.568
IPC2_protein 4.533
IPC_protein 4.482
Toseland 4.291
ProMoST 4.609
Dawson 4.444
Bjellqvist 4.596
Wikipedia 4.368
Rodwell 4.304
Grimsley 4.19
Solomon 4.444
Lehninger 4.406
Nozaki 4.558
DTASelect 4.787
Thurlkill 4.317
EMBOSS 4.368
Sillero 4.596
Patrickios 3.923
IPC_peptide 4.444
IPC2_peptide 4.571
IPC2.peptide.svr19 4.581
Protein with the highest isoelectric point:
>tr|A0A1S6KZZ3|A0A1S6KZZ3_9CAUD Major capsid protein OS=Salmonella phage SEN8 OX=1647468 GN=SEN8_6 PE=4 SV=1
MM1 pKa = 7.37 KK2 pKa = 9.97 KK3 pKa = 10.38 KK4 pKa = 10.45 VMSVFFQLAWAALLVISLLYY24 pKa = 9.29 PRR26 pKa = 11.84 SGAPVLVGASVWVSCFLAWLLAALCAVGWFAGDD59 pKa = 3.41 RR60 pKa = 11.84 ARR62 pKa = 11.84 DD63 pKa = 3.54 EE64 pKa = 4.01 VRR66 pKa = 11.84 AALIKK71 pKa = 10.38 FRR73 pKa = 11.84 AHH75 pKa = 5.83 PVKK78 pKa = 10.2 PVRR81 pKa = 11.84 TWAIRR86 pKa = 11.84 LLIVLCLAFSGWVITLVFYY105 pKa = 11.32 LLTLVLYY112 pKa = 9.81 QIARR116 pKa = 11.84 SQLHH120 pKa = 6.13 EE121 pKa = 4.36 PMAAA125 pKa = 3.23
Molecular weight: 13.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.604
IPC_protein 10.058
Toseland 10.379
ProMoST 10.043
Dawson 10.526
Bjellqvist 10.233
Wikipedia 10.701
Rodwell 10.862
Grimsley 10.584
Solomon 10.584
Lehninger 10.555
Nozaki 10.409
DTASelect 10.204
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.452
Patrickios 10.643
IPC_peptide 10.584
IPC2_peptide 9.385
IPC2.peptide.svr19 8.457
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
11156
39
935
227.7
25.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.537 ± 0.636
1.156 ± 0.163
5.791 ± 0.234
6.203 ± 0.363
3.155 ± 0.201
6.633 ± 0.422
1.936 ± 0.211
5.002 ± 0.243
5.208 ± 0.285
9.86 ± 0.294
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.591 ± 0.149
4.616 ± 0.296
4.213 ± 0.143
4.249 ± 0.212
6.517 ± 0.442
6.194 ± 0.189
5.88 ± 0.339
6.678 ± 0.279
1.721 ± 0.146
2.859 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here