Cotton leaf curl Burewala virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus

Average proteome isoelectric point is 8.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K8ESZ7|K8ESZ7_9GEMI Capsid protein OS=Cotton leaf curl Burewala virus OX=620894 GN=CP PE=3 SV=1
MM1 pKa = 7.66AAPKK5 pKa = 10.08RR6 pKa = 11.84FKK8 pKa = 10.87VQAKK12 pKa = 9.81NYY14 pKa = 9.23FITYY18 pKa = 6.67PQCSLTKK25 pKa = 10.54EE26 pKa = 4.03EE27 pKa = 5.09ALSQIQAINTPSNKK41 pKa = 9.74KK42 pKa = 9.85YY43 pKa = 10.49IKK45 pKa = 10.16LCRR48 pKa = 11.84EE49 pKa = 3.86LHH51 pKa = 6.34EE52 pKa = 5.81DD53 pKa = 4.2GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 4.8GKK67 pKa = 9.67FVCTNNRR74 pKa = 11.84FFDD77 pKa = 4.02LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.46PNIQGVKK95 pKa = 9.81SSSDD99 pKa = 3.09VKK101 pKa = 11.12AYY103 pKa = 9.68IDD105 pKa = 3.75KK106 pKa = 11.33DD107 pKa = 3.43GDD109 pKa = 3.69TLEE112 pKa = 4.14WGEE115 pKa = 3.84FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.05AYY134 pKa = 10.69AAALNAGTKK143 pKa = 9.96SEE145 pKa = 3.99ALRR148 pKa = 11.84VIKK151 pKa = 10.2EE152 pKa = 3.88LAPKK156 pKa = 10.6DD157 pKa = 3.7FVLQFHH163 pKa = 6.7NLNANLDD170 pKa = 4.29RR171 pKa = 11.84IFQEE175 pKa = 4.33PPAPYY180 pKa = 9.43ISPFSPSSFDD190 pKa = 3.4QVPEE194 pKa = 3.89EE195 pKa = 4.29LEE197 pKa = 3.84VWVIDD202 pKa = 3.97NVVDD206 pKa = 4.02AAARR210 pKa = 11.84PLRR213 pKa = 11.84PRR215 pKa = 11.84SIVIEE220 pKa = 4.26GDD222 pKa = 3.21SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 4.79DD262 pKa = 4.33VDD264 pKa = 3.73PHH266 pKa = 6.08FLKK269 pKa = 10.67HH270 pKa = 5.9FKK272 pKa = 10.69EE273 pKa = 4.43FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.27KK312 pKa = 10.62EE313 pKa = 4.11FIDD316 pKa = 4.16EE317 pKa = 4.52EE318 pKa = 4.78KK319 pKa = 10.24NTALKK324 pKa = 10.38NWAVKK329 pKa = 10.17NAIFITLDD337 pKa = 3.05RR338 pKa = 11.84PLYY341 pKa = 10.76SGTNQSTAQGSEE353 pKa = 3.9EE354 pKa = 3.88AQQEE358 pKa = 4.37EE359 pKa = 4.97EE360 pKa = 4.31SRR362 pKa = 11.84SS363 pKa = 3.6

Molecular weight: 40.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K8F8A6|K8F8A6_9GEMI Replication enhancer OS=Cotton leaf curl Burewala virus OX=620894 GN=REn PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.79VSRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 10.71AKK42 pKa = 10.29AWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.87PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.1SRR80 pKa = 11.84HH81 pKa = 6.13DD82 pKa = 3.26IQHH85 pKa = 6.35IGKK88 pKa = 8.97VMCVSDD94 pKa = 3.59VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.38NVHH172 pKa = 6.5RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 8.77QVLRR180 pKa = 11.84KK181 pKa = 9.41WYY183 pKa = 8.08ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.65KK201 pKa = 9.58FIRR204 pKa = 11.84VNNYY208 pKa = 7.74VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1106

54

363

184.3

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.239 ± 0.746

1.808 ± 0.566

4.521 ± 0.749

4.521 ± 0.767

4.25 ± 0.525

4.521 ± 0.616

2.984 ± 0.375

5.787 ± 0.737

6.691 ± 0.497

6.781 ± 0.973

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.596

5.515 ± 0.605

6.058 ± 0.531

5.696 ± 0.845

6.148 ± 1.024

8.59 ± 2.114

6.329 ± 1.011

6.058 ± 1.56

1.356 ± 0.129

3.888 ± 0.685
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski