Sulfurovum lithotrophicum
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 666 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T4CQ15|A0A2T4CQ15_9PROT Long-chain fatty acid transporter (Fragment) OS=Sulfurovum lithotrophicum OX=206403 GN=C9926_01835 PE=3 SV=1
KKK2 pKa = 10.16 QGIAYYY8 pKa = 7.73 DDD10 pKa = 3.36 DDD12 pKa = 2.7 DDD14 pKa = 4.36 NVDDD18 pKa = 3.4 YY19 pKa = 10.63 ASVVTDDD26 pKa = 4.37 DD27 pKa = 4.81 ALGSQDDD34 pKa = 4.38 DD35 pKa = 4.3 TEEE38 pKa = 4.27 TCPNCSTTPVCPIDDD53 pKa = 3.76 TPICPEEE60 pKa = 3.72 TTEEE64 pKa = 3.84 STPNTPAMSGTTIIPLLGGEEE85 pKa = 4.39 LVSFSQATHHH95 pKa = 5.69 QVTLNDDD102 pKa = 4.44 EEE104 pKa = 5.47 DD105 pKa = 3.52 DD106 pKa = 3.83 LVYYY110 pKa = 10.18 PNPGYYY116 pKa = 10.94 GTDDD120 pKa = 2.5 FTYYY124 pKa = 8.23 TRR126 pKa = 11.84 DDD128 pKa = 3.03 NGNEEE133 pKa = 4.21 VRR135 pKa = 11.84 TATVTVAPVITSRR148 pKa = 11.84 GEEE151 pKa = 3.84 LDDD154 pKa = 3.11 LGIGEEE160 pKa = 4.25 LISFTQPSYYY170 pKa = 11.45 VIVLDDD176 pKa = 5.69 DD177 pKa = 4.44 GTPDDD182 pKa = 6.04 DD183 pKa = 4.5 TDDD186 pKa = 3.99 DD187 pKa = 3.98 LRR189 pKa = 11.84 YYY191 pKa = 9.65 PNDDD195 pKa = 4.37 YYY197 pKa = 11.57 GLDDD201 pKa = 3.15 FSYYY205 pKa = 9.79 IRR207 pKa = 11.84 DDD209 pKa = 3.37 AGNVVTKKK217 pKa = 10.58 MTFTVDDD224 pKa = 3.23 VKKK227 pKa = 10.18 DDD229 pKa = 4.72 GDDD232 pKa = 3.71 LDDD235 pKa = 4.89 KK236 pKa = 11.15 SIMLLMFFTCLIGLYYY252 pKa = 7.8 YY253 pKa = 10.12 RR254 pKa = 11.84 RR255 pKa = 11.84 EEE257 pKa = 3.64 EE258 pKa = 3.98 SIKKK262 pKa = 10.18 KK263 pKa = 9.94 EE264 pKa = 3.92
Molecular weight: 28.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.683
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.567
ProMoST 4.012
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.923
Patrickios 1.189
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A2T4CP98|A0A2T4CP98_9PROT Aspartate transcarbamylase (Fragment) OS=Sulfurovum lithotrophicum OX=206403 GN=C9926_03110 PE=3 SV=1
GG1 pKa = 7.41 IGLTTARR8 pKa = 11.84 EE9 pKa = 4.13 ILDD12 pKa = 4.09 KK13 pKa = 11.36 ADD15 pKa = 5.16 LPYY18 pKa = 11.29 QMNSDD23 pKa = 4.02 DD24 pKa = 5.04 LSGEE28 pKa = 3.95 EE29 pKa = 3.5 ITRR32 pKa = 11.84 IRR34 pKa = 11.84 KK35 pKa = 9.32 IIEE38 pKa = 3.56 NDD40 pKa = 3.54 YY41 pKa = 11.08 VVEE44 pKa = 4.11 GDD46 pKa = 3.17 KK47 pKa = 10.91 RR48 pKa = 11.84 RR49 pKa = 11.84 EE50 pKa = 3.56 VSMDD54 pKa = 2.41 IRR56 pKa = 11.84 RR57 pKa = 11.84 LMDD60 pKa = 4.05 LGCYY64 pKa = 9.41 RR65 pKa = 11.84 GRR67 pKa = 11.84 RR68 pKa = 11.84 HH69 pKa = 6.29 RR70 pKa = 11.84 QGLPCRR76 pKa = 11.84 GQRR79 pKa = 11.84 TKK81 pKa = 10.95 TNARR85 pKa = 11.84 TRR87 pKa = 11.84 KK88 pKa = 7.95 GPRR91 pKa = 11.84 RR92 pKa = 11.84 GSVCRR97 pKa = 3.94
Molecular weight: 11.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.37
IPC_protein 9.999
Toseland 10.321
ProMoST 10.262
Dawson 10.467
Bjellqvist 10.204
Wikipedia 10.672
Rodwell 10.613
Grimsley 10.526
Solomon 10.57
Lehninger 10.526
Nozaki 10.365
DTASelect 10.175
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.409
Patrickios 10.35
IPC_peptide 10.57
IPC2_peptide 9.37
IPC2.peptide.svr19 8.522
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
666
0
666
170421
34
1101
255.9
28.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.057 ± 0.107
0.911 ± 0.036
5.78 ± 0.071
7.29 ± 0.09
4.597 ± 0.078
6.123 ± 0.106
2.212 ± 0.048
7.781 ± 0.097
8.238 ± 0.114
9.681 ± 0.109
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.797 ± 0.052
4.709 ± 0.087
3.155 ± 0.05
3.041 ± 0.052
3.488 ± 0.072
6.166 ± 0.082
5.793 ± 0.085
6.372 ± 0.085
0.811 ± 0.031
3.998 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here