Amino acid dipepetide frequency for Paenibacillus phage Eltigre

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.65AlaAla: 4.65 ± 0.984
0.498AlaCys: 0.498 ± 0.17
4.151AlaAsp: 4.151 ± 0.713
6.642AlaGlu: 6.642 ± 0.646
1.827AlaPhe: 1.827 ± 0.341
4.151AlaGly: 4.151 ± 0.584
0.913AlaHis: 0.913 ± 0.274
4.567AlaIle: 4.567 ± 0.793
5.812AlaLys: 5.812 ± 0.669
5.48AlaLeu: 5.48 ± 0.576
1.245AlaMet: 1.245 ± 0.291
2.906AlaAsn: 2.906 ± 0.516
1.162AlaPro: 1.162 ± 0.34
1.993AlaGln: 1.993 ± 0.419
2.076AlaArg: 2.076 ± 0.428
4.234AlaSer: 4.234 ± 0.635
3.902AlaThr: 3.902 ± 0.733
3.736AlaVal: 3.736 ± 0.568
1.245AlaTrp: 1.245 ± 0.315
2.408AlaTyr: 2.408 ± 0.429
0.0AlaXaa: 0.0 ± 0.0
Cys
0.415CysAla: 0.415 ± 0.2
0.249CysCys: 0.249 ± 0.127
0.581CysAsp: 0.581 ± 0.199
0.498CysGlu: 0.498 ± 0.227
0.249CysPhe: 0.249 ± 0.128
0.83CysGly: 0.83 ± 0.262
0.249CysHis: 0.249 ± 0.128
0.415CysIle: 0.415 ± 0.19
1.328CysLys: 1.328 ± 0.361
0.747CysLeu: 0.747 ± 0.272
0.332CysMet: 0.332 ± 0.146
0.332CysAsn: 0.332 ± 0.184
0.581CysPro: 0.581 ± 0.245
0.249CysGln: 0.249 ± 0.155
0.581CysArg: 0.581 ± 0.188
0.581CysSer: 0.581 ± 0.189
0.332CysThr: 0.332 ± 0.169
0.498CysVal: 0.498 ± 0.222
0.249CysTrp: 0.249 ± 0.185
0.415CysTyr: 0.415 ± 0.189
0.0CysXaa: 0.0 ± 0.0
Asp
3.487AspAla: 3.487 ± 0.527
0.664AspCys: 0.664 ± 0.292
4.318AspAsp: 4.318 ± 0.719
4.318AspGlu: 4.318 ± 0.783
2.906AspPhe: 2.906 ± 0.578
3.736AspGly: 3.736 ± 0.566
0.747AspHis: 0.747 ± 0.23
3.985AspIle: 3.985 ± 0.433
3.902AspLys: 3.902 ± 0.624
5.397AspLeu: 5.397 ± 0.705
2.242AspMet: 2.242 ± 0.423
1.744AspAsn: 1.744 ± 0.358
2.242AspPro: 2.242 ± 0.406
2.076AspGln: 2.076 ± 0.427
2.823AspArg: 2.823 ± 0.522
2.74AspSer: 2.74 ± 0.454
3.072AspThr: 3.072 ± 0.435
3.819AspVal: 3.819 ± 0.597
0.747AspTrp: 0.747 ± 0.26
1.993AspTyr: 1.993 ± 0.509
0.0AspXaa: 0.0 ± 0.0
Glu
5.48GluAla: 5.48 ± 0.719
0.415GluCys: 0.415 ± 0.226
5.148GluAsp: 5.148 ± 0.815
6.144GluGlu: 6.144 ± 0.829
2.74GluPhe: 2.74 ± 0.403
3.321GluGly: 3.321 ± 0.419
1.411GluHis: 1.411 ± 0.445
6.642GluIle: 6.642 ± 0.824
8.386GluLys: 8.386 ± 0.788
7.057GluLeu: 7.057 ± 0.761
4.816GluMet: 4.816 ± 0.553
3.819GluAsn: 3.819 ± 0.664
2.076GluPro: 2.076 ± 0.406
4.401GluGln: 4.401 ± 0.691
4.151GluArg: 4.151 ± 0.616
4.401GluSer: 4.401 ± 0.54
4.816GluThr: 4.816 ± 0.596
5.148GluVal: 5.148 ± 0.74
0.996GluTrp: 0.996 ± 0.315
3.57GluTyr: 3.57 ± 0.567
0.0GluXaa: 0.0 ± 0.0
Phe
1.993PheAla: 1.993 ± 0.365
0.83PheCys: 0.83 ± 0.251
2.325PheAsp: 2.325 ± 0.469
3.487PheGlu: 3.487 ± 0.527
1.162PhePhe: 1.162 ± 0.338
2.325PheGly: 2.325 ± 0.401
1.245PheHis: 1.245 ± 0.351
3.072PheIle: 3.072 ± 0.441
2.989PheLys: 2.989 ± 0.612
3.487PheLeu: 3.487 ± 0.571
1.162PheMet: 1.162 ± 0.333
2.076PheAsn: 2.076 ± 0.375
0.83PhePro: 0.83 ± 0.253
1.411PheGln: 1.411 ± 0.326
0.83PheArg: 0.83 ± 0.276
2.491PheSer: 2.491 ± 0.491
1.744PheThr: 1.744 ± 0.3
2.159PheVal: 2.159 ± 0.487
0.498PheTrp: 0.498 ± 0.206
1.328PheTyr: 1.328 ± 0.325
0.0PheXaa: 0.0 ± 0.0
Gly
3.321GlyAla: 3.321 ± 0.648
0.747GlyCys: 0.747 ± 0.333
2.989GlyAsp: 2.989 ± 0.589
4.816GlyGlu: 4.816 ± 0.754
2.823GlyPhe: 2.823 ± 0.499
2.657GlyGly: 2.657 ± 0.561
0.913GlyHis: 0.913 ± 0.364
5.563GlyIle: 5.563 ± 0.892
6.559GlyLys: 6.559 ± 0.533
4.567GlyLeu: 4.567 ± 0.921
1.578GlyMet: 1.578 ± 0.562
3.238GlyAsn: 3.238 ± 0.493
0.581GlyPro: 0.581 ± 0.232
2.242GlyGln: 2.242 ± 0.399
2.989GlyArg: 2.989 ± 0.545
3.238GlySer: 3.238 ± 0.583
2.242GlyThr: 2.242 ± 0.594
3.57GlyVal: 3.57 ± 0.488
0.913GlyTrp: 0.913 ± 0.212
2.408GlyTyr: 2.408 ± 0.505
0.0GlyXaa: 0.0 ± 0.0
His
1.245HisAla: 1.245 ± 0.347
0.249HisCys: 0.249 ± 0.133
0.913HisAsp: 0.913 ± 0.365
1.495HisGlu: 1.495 ± 0.411
0.913HisPhe: 0.913 ± 0.248
0.415HisGly: 0.415 ± 0.174
0.415HisHis: 0.415 ± 0.167
1.91HisIle: 1.91 ± 0.372
0.996HisLys: 0.996 ± 0.36
2.159HisLeu: 2.159 ± 0.509
0.332HisMet: 0.332 ± 0.15
0.913HisAsn: 0.913 ± 0.272
0.664HisPro: 0.664 ± 0.205
0.664HisGln: 0.664 ± 0.189
0.996HisArg: 0.996 ± 0.212
1.245HisSer: 1.245 ± 0.362
0.415HisThr: 0.415 ± 0.18
1.411HisVal: 1.411 ± 0.383
0.166HisTrp: 0.166 ± 0.101
0.415HisTyr: 0.415 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
4.068IleAla: 4.068 ± 0.628
0.83IleCys: 0.83 ± 0.287
4.65IleAsp: 4.65 ± 0.719
6.974IleGlu: 6.974 ± 0.68
2.989IlePhe: 2.989 ± 0.461
3.653IleGly: 3.653 ± 0.615
1.495IleHis: 1.495 ± 0.338
3.736IleIle: 3.736 ± 0.684
6.891IleLys: 6.891 ± 1.03
5.895IleLeu: 5.895 ± 0.785
1.411IleMet: 1.411 ± 0.368
2.989IleAsn: 2.989 ± 0.511
3.321IlePro: 3.321 ± 0.454
3.072IleGln: 3.072 ± 0.552
3.57IleArg: 3.57 ± 0.592
3.819IleSer: 3.819 ± 0.548
4.068IleThr: 4.068 ± 0.418
4.899IleVal: 4.899 ± 0.638
0.913IleTrp: 0.913 ± 0.271
3.155IleTyr: 3.155 ± 0.497
0.0IleXaa: 0.0 ± 0.0
Lys
6.061LysAla: 6.061 ± 0.644
0.747LysCys: 0.747 ± 0.221
4.068LysAsp: 4.068 ± 0.508
9.216LysGlu: 9.216 ± 0.912
2.491LysPhe: 2.491 ± 0.333
6.061LysGly: 6.061 ± 0.772
1.411LysHis: 1.411 ± 0.232
6.393LysIle: 6.393 ± 0.7
8.884LysLys: 8.884 ± 1.218
6.393LysLeu: 6.393 ± 0.719
3.404LysMet: 3.404 ± 0.476
4.401LysAsn: 4.401 ± 0.683
2.408LysPro: 2.408 ± 0.488
4.484LysGln: 4.484 ± 0.645
5.48LysArg: 5.48 ± 0.832
5.729LysSer: 5.729 ± 0.675
5.48LysThr: 5.48 ± 0.772
3.653LysVal: 3.653 ± 0.494
1.245LysTrp: 1.245 ± 0.314
2.823LysTyr: 2.823 ± 0.38
0.0LysXaa: 0.0 ± 0.0
Leu
6.31LeuAla: 6.31 ± 0.717
0.747LeuCys: 0.747 ± 0.272
4.816LeuAsp: 4.816 ± 0.585
7.307LeuGlu: 7.307 ± 0.711
2.574LeuPhe: 2.574 ± 0.416
4.65LeuGly: 4.65 ± 0.559
1.91LeuHis: 1.91 ± 0.393
4.899LeuIle: 4.899 ± 0.51
6.891LeuLys: 6.891 ± 0.657
6.476LeuLeu: 6.476 ± 0.814
1.495LeuMet: 1.495 ± 0.32
4.401LeuAsn: 4.401 ± 0.672
2.989LeuPro: 2.989 ± 0.452
4.484LeuGln: 4.484 ± 0.586
3.57LeuArg: 3.57 ± 0.507
6.227LeuSer: 6.227 ± 0.778
5.48LeuThr: 5.48 ± 0.644
3.985LeuVal: 3.985 ± 0.537
0.913LeuTrp: 0.913 ± 0.342
2.74LeuTyr: 2.74 ± 0.494
0.0LeuXaa: 0.0 ± 0.0
Met
1.993MetAla: 1.993 ± 0.396
0.083MetCys: 0.083 ± 0.089
1.661MetAsp: 1.661 ± 0.354
2.74MetGlu: 2.74 ± 0.398
1.495MetPhe: 1.495 ± 0.308
1.162MetGly: 1.162 ± 0.295
0.166MetHis: 0.166 ± 0.111
2.242MetIle: 2.242 ± 0.417
3.736MetLys: 3.736 ± 0.558
2.823MetLeu: 2.823 ± 0.585
0.83MetMet: 0.83 ± 0.245
2.325MetAsn: 2.325 ± 0.438
0.996MetPro: 0.996 ± 0.274
1.328MetGln: 1.328 ± 0.277
1.079MetArg: 1.079 ± 0.35
2.242MetSer: 2.242 ± 0.418
1.495MetThr: 1.495 ± 0.349
1.079MetVal: 1.079 ± 0.396
0.249MetTrp: 0.249 ± 0.145
0.913MetTyr: 0.913 ± 0.29
0.0MetXaa: 0.0 ± 0.0
Asn
1.993AsnAla: 1.993 ± 0.361
0.581AsnCys: 0.581 ± 0.202
2.076AsnAsp: 2.076 ± 0.465
3.321AsnGlu: 3.321 ± 0.592
1.245AsnPhe: 1.245 ± 0.31
3.736AsnGly: 3.736 ± 0.584
1.079AsnHis: 1.079 ± 0.288
3.238AsnIle: 3.238 ± 0.525
3.653AsnLys: 3.653 ± 0.589
4.068AsnLeu: 4.068 ± 0.635
1.495AsnMet: 1.495 ± 0.512
2.491AsnAsn: 2.491 ± 0.608
2.325AsnPro: 2.325 ± 0.507
1.661AsnGln: 1.661 ± 0.417
3.321AsnArg: 3.321 ± 0.54
2.74AsnSer: 2.74 ± 0.428
2.325AsnThr: 2.325 ± 0.44
2.823AsnVal: 2.823 ± 0.507
0.747AsnTrp: 0.747 ± 0.225
1.744AsnTyr: 1.744 ± 0.347
0.0AsnXaa: 0.0 ± 0.0
Pro
1.91ProAla: 1.91 ± 0.336
0.083ProCys: 0.083 ± 0.084
1.578ProAsp: 1.578 ± 0.326
2.823ProGlu: 2.823 ± 0.75
1.162ProPhe: 1.162 ± 0.294
2.325ProGly: 2.325 ± 0.403
0.913ProHis: 0.913 ± 0.254
2.242ProIle: 2.242 ± 0.386
3.321ProLys: 3.321 ± 0.667
2.574ProLeu: 2.574 ± 0.411
0.664ProMet: 0.664 ± 0.252
1.744ProAsn: 1.744 ± 0.394
0.913ProPro: 0.913 ± 0.26
0.747ProGln: 0.747 ± 0.342
0.498ProArg: 0.498 ± 0.195
2.823ProSer: 2.823 ± 0.581
1.827ProThr: 1.827 ± 0.343
2.076ProVal: 2.076 ± 0.525
0.498ProTrp: 0.498 ± 0.185
1.162ProTyr: 1.162 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
3.321GlnAla: 3.321 ± 0.539
0.249GlnCys: 0.249 ± 0.155
1.993GlnAsp: 1.993 ± 0.323
4.068GlnGlu: 4.068 ± 0.64
1.661GlnPhe: 1.661 ± 0.311
2.574GlnGly: 2.574 ± 0.588
1.079GlnHis: 1.079 ± 0.275
2.491GlnIle: 2.491 ± 0.423
3.487GlnLys: 3.487 ± 0.598
3.653GlnLeu: 3.653 ± 0.669
1.91GlnMet: 1.91 ± 0.466
1.328GlnAsn: 1.328 ± 0.345
1.827GlnPro: 1.827 ± 0.477
1.993GlnGln: 1.993 ± 0.503
2.657GlnArg: 2.657 ± 0.499
2.242GlnSer: 2.242 ± 0.392
2.574GlnThr: 2.574 ± 0.424
1.91GlnVal: 1.91 ± 0.368
0.415GlnTrp: 0.415 ± 0.209
1.162GlnTyr: 1.162 ± 0.339
0.0GlnXaa: 0.0 ± 0.0
Arg
2.657ArgAla: 2.657 ± 0.415
0.498ArgCys: 0.498 ± 0.241
2.989ArgAsp: 2.989 ± 0.413
3.321ArgGlu: 3.321 ± 0.539
1.411ArgPhe: 1.411 ± 0.331
2.657ArgGly: 2.657 ± 0.519
0.747ArgHis: 0.747 ± 0.238
2.989ArgIle: 2.989 ± 0.578
4.816ArgLys: 4.816 ± 0.857
4.234ArgLeu: 4.234 ± 0.607
1.495ArgMet: 1.495 ± 0.31
2.325ArgAsn: 2.325 ± 0.474
1.328ArgPro: 1.328 ± 0.337
2.574ArgGln: 2.574 ± 0.369
2.408ArgArg: 2.408 ± 0.431
2.242ArgSer: 2.242 ± 0.307
2.408ArgThr: 2.408 ± 0.468
2.906ArgVal: 2.906 ± 0.483
0.415ArgTrp: 0.415 ± 0.188
1.827ArgTyr: 1.827 ± 0.349
0.0ArgXaa: 0.0 ± 0.0
Ser
5.231SerAla: 5.231 ± 0.936
0.581SerCys: 0.581 ± 0.186
3.321SerAsp: 3.321 ± 0.498
5.231SerGlu: 5.231 ± 0.649
2.574SerPhe: 2.574 ± 0.447
4.068SerGly: 4.068 ± 0.677
0.913SerHis: 0.913 ± 0.327
6.476SerIle: 6.476 ± 0.788
4.234SerLys: 4.234 ± 0.552
5.397SerLeu: 5.397 ± 0.848
1.661SerMet: 1.661 ± 0.353
2.159SerAsn: 2.159 ± 0.408
2.491SerPro: 2.491 ± 0.399
2.574SerGln: 2.574 ± 0.578
2.325SerArg: 2.325 ± 0.457
3.404SerSer: 3.404 ± 0.594
3.653SerThr: 3.653 ± 0.578
3.985SerVal: 3.985 ± 0.778
0.249SerTrp: 0.249 ± 0.152
1.993SerTyr: 1.993 ± 0.322
0.0SerXaa: 0.0 ± 0.0
Thr
3.902ThrAla: 3.902 ± 0.665
0.332ThrCys: 0.332 ± 0.148
2.325ThrAsp: 2.325 ± 0.459
3.57ThrGlu: 3.57 ± 0.527
2.159ThrPhe: 2.159 ± 0.378
4.068ThrGly: 4.068 ± 0.544
0.913ThrHis: 0.913 ± 0.32
4.068ThrIle: 4.068 ± 0.496
5.314ThrLys: 5.314 ± 0.649
4.068ThrLeu: 4.068 ± 0.706
1.578ThrMet: 1.578 ± 0.398
1.993ThrAsn: 1.993 ± 0.376
2.574ThrPro: 2.574 ± 0.485
2.574ThrGln: 2.574 ± 0.527
1.993ThrArg: 1.993 ± 0.369
4.068ThrSer: 4.068 ± 0.542
2.325ThrThr: 2.325 ± 0.468
4.65ThrVal: 4.65 ± 0.615
0.249ThrTrp: 0.249 ± 0.147
2.657ThrTyr: 2.657 ± 0.478
0.0ThrXaa: 0.0 ± 0.0
Val
3.321ValAla: 3.321 ± 0.503
0.498ValCys: 0.498 ± 0.214
3.819ValAsp: 3.819 ± 0.569
5.314ValGlu: 5.314 ± 0.632
2.823ValPhe: 2.823 ± 0.488
2.823ValGly: 2.823 ± 0.638
0.581ValHis: 0.581 ± 0.298
4.234ValIle: 4.234 ± 0.592
5.397ValLys: 5.397 ± 0.696
3.819ValLeu: 3.819 ± 0.46
1.495ValMet: 1.495 ± 0.34
2.408ValAsn: 2.408 ± 0.478
1.578ValPro: 1.578 ± 0.402
1.91ValGln: 1.91 ± 0.345
2.325ValArg: 2.325 ± 0.394
4.982ValSer: 4.982 ± 0.536
4.484ValThr: 4.484 ± 0.514
3.736ValVal: 3.736 ± 0.518
1.827ValTrp: 1.827 ± 0.895
2.076ValTyr: 2.076 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
0.415TrpAla: 0.415 ± 0.159
0.166TrpCys: 0.166 ± 0.128
0.83TrpAsp: 0.83 ± 0.302
0.996TrpGlu: 0.996 ± 0.241
0.913TrpPhe: 0.913 ± 0.24
0.664TrpGly: 0.664 ± 0.216
0.415TrpHis: 0.415 ± 0.16
0.498TrpIle: 0.498 ± 0.166
0.913TrpLys: 0.913 ± 0.247
1.578TrpLeu: 1.578 ± 0.317
0.166TrpMet: 0.166 ± 0.12
1.827TrpAsn: 1.827 ± 1.038
0.0TrpPro: 0.0 ± 0.0
0.664TrpGln: 0.664 ± 0.198
0.498TrpArg: 0.498 ± 0.237
0.913TrpSer: 0.913 ± 0.259
0.415TrpThr: 0.415 ± 0.191
0.581TrpVal: 0.581 ± 0.226
0.166TrpTrp: 0.166 ± 0.123
0.498TrpTyr: 0.498 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.827TyrAla: 1.827 ± 0.386
0.664TyrCys: 0.664 ± 0.265
2.491TyrAsp: 2.491 ± 0.52
2.657TyrGlu: 2.657 ± 0.385
1.328TyrPhe: 1.328 ± 0.298
2.076TyrGly: 2.076 ± 0.482
0.415TyrHis: 0.415 ± 0.194
2.74TyrIle: 2.74 ± 0.483
3.321TyrLys: 3.321 ± 0.501
2.989TyrLeu: 2.989 ± 0.495
1.245TyrMet: 1.245 ± 0.355
1.245TyrAsn: 1.245 ± 0.33
0.996TyrPro: 0.996 ± 0.284
1.495TyrGln: 1.495 ± 0.297
2.076TyrArg: 2.076 ± 0.466
2.159TyrSer: 2.159 ± 0.428
2.325TyrThr: 2.325 ± 0.39
2.823TyrVal: 2.823 ± 0.434
0.415TyrTrp: 0.415 ± 0.169
1.578TyrTyr: 1.578 ± 0.314
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12045 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski