Callinectes ornatus blue crab associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RL45|A0A0K1RL45_9CIRC Putative replication initiation protein OS=Callinectes ornatus blue crab associated circular virus OX=1692245 PE=4 SV=1
MM1 pKa = 7.26AQAKK5 pKa = 9.57RR6 pKa = 11.84WVFTLNNYY14 pKa = 9.94SGADD18 pKa = 3.62EE19 pKa = 4.12QLLEE23 pKa = 3.99QLSRR27 pKa = 11.84STDD30 pKa = 3.48VVTLIYY36 pKa = 10.42GRR38 pKa = 11.84EE39 pKa = 3.98VAPGTGTNHH48 pKa = 5.72LQGMIIFATRR58 pKa = 11.84KK59 pKa = 9.14RR60 pKa = 11.84LRR62 pKa = 11.84QIRR65 pKa = 11.84NYY67 pKa = 10.49PPFQRR72 pKa = 11.84AHH74 pKa = 6.55LEE76 pKa = 4.29VMRR79 pKa = 11.84STPARR84 pKa = 11.84ARR86 pKa = 11.84EE87 pKa = 3.92YY88 pKa = 10.55CIKK91 pKa = 11.0DD92 pKa = 3.13GDD94 pKa = 3.93FVEE97 pKa = 4.77YY98 pKa = 10.88GEE100 pKa = 4.21PVEE103 pKa = 4.45EE104 pKa = 3.99NRR106 pKa = 11.84QGRR109 pKa = 11.84RR110 pKa = 11.84NDD112 pKa = 2.8IHH114 pKa = 8.24DD115 pKa = 4.41IIAWLDD121 pKa = 3.46LFIEE125 pKa = 4.55EE126 pKa = 4.39NNRR129 pKa = 11.84PPTQEE134 pKa = 4.09EE135 pKa = 4.68VAQLQPIAMLRR146 pKa = 11.84YY147 pKa = 9.67RR148 pKa = 11.84NFMDD152 pKa = 4.89LARR155 pKa = 11.84LRR157 pKa = 11.84APQPQLIRR165 pKa = 11.84NGVARR170 pKa = 11.84EE171 pKa = 4.08GWQMDD176 pKa = 4.26LEE178 pKa = 4.57HH179 pKa = 7.54ALDD182 pKa = 4.3GDD184 pKa = 3.73APDD187 pKa = 3.48RR188 pKa = 11.84HH189 pKa = 6.94ILFYY193 pKa = 11.02VDD195 pKa = 3.33PAGGNGKK202 pKa = 7.29TWFQQYY208 pKa = 11.21LLTKK212 pKa = 8.89YY213 pKa = 9.63PRR215 pKa = 11.84RR216 pKa = 11.84VQILGTGNFADD227 pKa = 3.98CAYY230 pKa = 10.54AVDD233 pKa = 4.03EE234 pKa = 4.78SKK236 pKa = 11.09DD237 pKa = 3.59IIISPEE243 pKa = 3.63MTLWLPAIWLSVCEE257 pKa = 4.69AEE259 pKa = 6.0LNSQTT264 pKa = 4.5

Molecular weight: 30.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RL45|A0A0K1RL45_9CIRC Putative replication initiation protein OS=Callinectes ornatus blue crab associated circular virus OX=1692245 PE=4 SV=1
MM1 pKa = 7.24SAIVPFARR9 pKa = 11.84YY10 pKa = 8.71AFPGARR16 pKa = 11.84RR17 pKa = 11.84AAAKK21 pKa = 10.53ALIRR25 pKa = 11.84TGKK28 pKa = 7.7ITPRR32 pKa = 11.84QYY34 pKa = 9.6MAAYY38 pKa = 9.24KK39 pKa = 9.98IGRR42 pKa = 11.84AAYY45 pKa = 8.39RR46 pKa = 11.84WRR48 pKa = 11.84GIFKK52 pKa = 10.45SKK54 pKa = 10.19GYY56 pKa = 9.77QRR58 pKa = 11.84SKK60 pKa = 10.6RR61 pKa = 11.84RR62 pKa = 11.84FVRR65 pKa = 11.84NVRR68 pKa = 11.84NVVRR72 pKa = 11.84NVRR75 pKa = 11.84SAQLKK80 pKa = 5.7TTHH83 pKa = 7.08LDD85 pKa = 3.49FASDD89 pKa = 3.46EE90 pKa = 4.21QFTNKK95 pKa = 9.71LNHH98 pKa = 6.0LHH100 pKa = 6.62HH101 pKa = 5.9VQPYY105 pKa = 8.17KK106 pKa = 10.63LVYY109 pKa = 10.57LGDD112 pKa = 4.22GDD114 pKa = 4.47NKK116 pKa = 10.29RR117 pKa = 11.84QSSTITCSGLRR128 pKa = 11.84IQFCFANTQPNGGQPQSHH146 pKa = 6.84LRR148 pKa = 11.84TDD150 pKa = 3.53YY151 pKa = 11.34DD152 pKa = 2.96ILRR155 pKa = 11.84FINSISTISEE165 pKa = 3.78ITCTQNLHH173 pKa = 5.28TPRR176 pKa = 11.84ILGQKK181 pKa = 9.51ILLKK185 pKa = 10.38PRR187 pKa = 11.84LTISTSRR194 pKa = 11.84IHH196 pKa = 7.81VEE198 pKa = 3.61

Molecular weight: 22.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

462

198

264

231.0

26.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.009 ± 0.259

1.299 ± 0.13

4.762 ± 1.036

4.762 ± 1.943

3.896 ± 0.389

5.844 ± 0.173

2.597 ± 0.561

7.143 ± 0.561

3.896 ± 1.295

8.658 ± 0.648

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.948 ± 0.561

4.978 ± 0.043

4.978 ± 0.561

6.277 ± 0.13

9.957 ± 0.691

4.762 ± 1.382

6.061 ± 0.907

4.762 ± 0.13

1.515 ± 0.604

3.896 ± 0.086
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski