Chayote yellow mosaic Benin betasatellite
Average proteome isoelectric point is 5.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191KVK0|A0A191KVK0_9VIRU BC1 OS=Chayote yellow mosaic Benin betasatellite OX=1736759 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 10.51 YY5 pKa = 9.02 KK6 pKa = 10.7 NKK8 pKa = 9.45 RR9 pKa = 11.84 GVEE12 pKa = 4.06 FTVDD16 pKa = 2.96 VFIKK20 pKa = 10.12 VGKK23 pKa = 9.07 VEE25 pKa = 4.18 CCIKK29 pKa = 9.95 VTATKK34 pKa = 10.23 DD35 pKa = 3.41 AYY37 pKa = 11.1 VSTSSFIIPYY47 pKa = 9.9 GYY49 pKa = 10.75 LEE51 pKa = 4.36 VIIPFDD57 pKa = 4.01 FNGTEE62 pKa = 3.94 QEE64 pKa = 3.69 ITNAIKK70 pKa = 10.53 AVFHH74 pKa = 5.2 TTKK77 pKa = 10.39 HH78 pKa = 5.64 RR79 pKa = 11.84 DD80 pKa = 2.79 IRR82 pKa = 11.84 AEE84 pKa = 4.03 EE85 pKa = 4.42 LVDD88 pKa = 4.19 AMDD91 pKa = 3.68 IVMSEE96 pKa = 3.99 NEE98 pKa = 4.37 NLIGMQIIEE107 pKa = 4.24 PHH109 pKa = 7.0 RR110 pKa = 11.84 ITSKK114 pKa = 9.9 TSVV117 pKa = 2.93
Molecular weight: 13.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.674
IPC2_protein 5.703
IPC_protein 5.677
Toseland 6.008
ProMoST 5.982
Dawson 5.881
Bjellqvist 5.893
Wikipedia 5.868
Rodwell 5.868
Grimsley 6.122
Solomon 5.881
Lehninger 5.868
Nozaki 6.122
DTASelect 6.3
Thurlkill 6.287
EMBOSS 6.249
Sillero 6.224
Patrickios 4.253
IPC_peptide 5.906
IPC2_peptide 6.198
IPC2.peptide.svr19 6.303
Protein with the highest isoelectric point:
>tr|A0A191KVK0|A0A191KVK0_9VIRU BC1 OS=Chayote yellow mosaic Benin betasatellite OX=1736759 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 10.51 YY5 pKa = 9.02 KK6 pKa = 10.7 NKK8 pKa = 9.45 RR9 pKa = 11.84 GVEE12 pKa = 4.06 FTVDD16 pKa = 2.96 VFIKK20 pKa = 10.12 VGKK23 pKa = 9.07 VEE25 pKa = 4.18 CCIKK29 pKa = 9.95 VTATKK34 pKa = 10.23 DD35 pKa = 3.41 AYY37 pKa = 11.1 VSTSSFIIPYY47 pKa = 9.9 GYY49 pKa = 10.75 LEE51 pKa = 4.36 VIIPFDD57 pKa = 4.01 FNGTEE62 pKa = 3.94 QEE64 pKa = 3.69 ITNAIKK70 pKa = 10.53 AVFHH74 pKa = 5.2 TTKK77 pKa = 10.39 HH78 pKa = 5.64 RR79 pKa = 11.84 DD80 pKa = 2.79 IRR82 pKa = 11.84 AEE84 pKa = 4.03 EE85 pKa = 4.42 LVDD88 pKa = 4.19 AMDD91 pKa = 3.68 IVMSEE96 pKa = 3.99 NEE98 pKa = 4.37 NLIGMQIIEE107 pKa = 4.24 PHH109 pKa = 7.0 RR110 pKa = 11.84 ITSKK114 pKa = 9.9 TSVV117 pKa = 2.93
Molecular weight: 13.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.674
IPC2_protein 5.703
IPC_protein 5.677
Toseland 6.008
ProMoST 5.982
Dawson 5.881
Bjellqvist 5.893
Wikipedia 5.868
Rodwell 5.868
Grimsley 6.122
Solomon 5.881
Lehninger 5.868
Nozaki 6.122
DTASelect 6.3
Thurlkill 6.287
EMBOSS 6.249
Sillero 6.224
Patrickios 4.253
IPC_peptide 5.906
IPC2_peptide 6.198
IPC2.peptide.svr19 6.303
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
117
117
117
117.0
13.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.128 ± 0.0
1.709 ± 0.0
5.128 ± 0.0
8.547 ± 0.0
5.128 ± 0.0
4.274 ± 0.0
2.564 ± 0.0
12.821 ± 0.0
8.547 ± 0.0
2.564 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.419 ± 0.0
4.274 ± 0.0
2.564 ± 0.0
1.709 ± 0.0
3.419 ± 0.0
5.128 ± 0.0
9.402 ± 0.0
10.256 ± 0.0
0.0 ± 0.0
3.419 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here