Staphylococcus phage phiSa2wa_st5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus; Staphylococcus virus st5

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6PDA4|A0A2I6PDA4_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSa2wa_st5 OX=2060951 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 2.96VDD20 pKa = 3.24YY21 pKa = 11.49QHH23 pKa = 7.01FDD25 pKa = 3.49DD26 pKa = 4.54VDD28 pKa = 3.67KK29 pKa = 11.4EE30 pKa = 4.28KK31 pKa = 10.38EE32 pKa = 4.13TLADD36 pKa = 3.57YY37 pKa = 10.81LYY39 pKa = 10.9NNPDD43 pKa = 3.71EE44 pKa = 4.28ILEE47 pKa = 3.92YY48 pKa = 11.25DD49 pKa = 3.43NLKK52 pKa = 9.69IRR54 pKa = 11.84NVNVEE59 pKa = 3.93VEE61 pKa = 4.1

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6PDF9|A0A2I6PDF9_9CAUD Holin OS=Staphylococcus phage phiSa2wa_st5 OX=2060951 GN=hol PE=4 SV=1
MM1 pKa = 7.54IKK3 pKa = 10.33KK4 pKa = 9.52LLWSTISWFYY14 pKa = 11.55VYY16 pKa = 10.47YY17 pKa = 9.12QSKK20 pKa = 9.42YY21 pKa = 10.26KK22 pKa = 10.83LFIICLVMNGFFSKK36 pKa = 10.93

Molecular weight: 4.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14483

25

2074

188.1

21.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.538 ± 0.662

0.532 ± 0.13

6.152 ± 0.238

7.416 ± 0.431

3.86 ± 0.234

5.986 ± 0.584

1.664 ± 0.136

7.312 ± 0.419

9.998 ± 0.501

7.906 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.141

7.022 ± 0.285

2.499 ± 0.216

3.818 ± 0.198

4.053 ± 0.177

6.325 ± 0.352

5.862 ± 0.232

5.648 ± 0.208

1.215 ± 0.102

4.495 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski