Pseudomonas pachastrellae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S8DDE1|A0A1S8DDE1_9PSED Uncharacterized protein OS=Pseudomonas pachastrellae OX=254161 GN=BXT89_16915 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 10.34RR3 pKa = 11.84FSALLLSSMLLSTAAQAQDD22 pKa = 3.3SFKK25 pKa = 10.87VCWSIYY31 pKa = 9.34AGWMPWAYY39 pKa = 9.76GAEE42 pKa = 4.02SGIVKK47 pKa = 9.99KK48 pKa = 9.67WADD51 pKa = 3.62KK52 pKa = 10.9YY53 pKa = 11.22DD54 pKa = 3.54INIDD58 pKa = 3.49VVQINDD64 pKa = 3.86YY65 pKa = 10.75IEE67 pKa = 5.44SINQYY72 pKa = 8.45TVGEE76 pKa = 4.29FDD78 pKa = 3.7ACTMTNMDD86 pKa = 4.98ALTIPAAGGVDD97 pKa = 3.65STALIVGDD105 pKa = 4.41FSNGNDD111 pKa = 4.1GIVLKK116 pKa = 11.12GEE118 pKa = 4.27SEE120 pKa = 3.89LAAIKK125 pKa = 9.67GQNVNLVEE133 pKa = 4.59LSVSHH138 pKa = 6.06YY139 pKa = 11.02LLARR143 pKa = 11.84ALDD146 pKa = 3.95SVGLSEE152 pKa = 6.34ADD154 pKa = 3.43VQVVNTSDD162 pKa = 3.46ADD164 pKa = 3.64MVAVYY169 pKa = 8.45GTDD172 pKa = 3.32DD173 pKa = 3.72VTSVATWNPLLSEE186 pKa = 4.75ISQQPSSTVVFNSAQIPGEE205 pKa = 4.27IIDD208 pKa = 4.62LLVINTDD215 pKa = 3.17TLAAHH220 pKa = 6.98PEE222 pKa = 3.97LGKK225 pKa = 10.71ALTGAWYY232 pKa = 9.7EE233 pKa = 4.07IMATMSIDD241 pKa = 3.61SEE243 pKa = 4.39QGVAARR249 pKa = 11.84SYY251 pKa = 8.95MAEE254 pKa = 3.85ASGTDD259 pKa = 3.47LAGYY263 pKa = 7.75EE264 pKa = 4.1AQLAATEE271 pKa = 4.24MFYY274 pKa = 10.76TPAAALEE281 pKa = 4.41LTNSPQLKK289 pKa = 8.2QTMQYY294 pKa = 9.82VAEE297 pKa = 4.38FSFDD301 pKa = 3.28HH302 pKa = 6.69GLLGDD307 pKa = 4.51GAPDD311 pKa = 3.22AGFIGVQMPAGDD323 pKa = 4.18YY324 pKa = 11.17GDD326 pKa = 3.99SSNLKK331 pKa = 10.29LRR333 pKa = 11.84FDD335 pKa = 3.76PTYY338 pKa = 10.37MQMAADD344 pKa = 3.83NQLL347 pKa = 3.03

Molecular weight: 37.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S8DE03|A0A1S8DE03_9PSED Uncharacterized protein OS=Pseudomonas pachastrellae OX=254161 GN=BXT89_16315 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.39GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3588

0

3588

1158881

23

2623

323.0

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.06 ± 0.053

1.028 ± 0.013

5.679 ± 0.031

6.046 ± 0.04

3.442 ± 0.022

7.819 ± 0.037

2.227 ± 0.02

4.691 ± 0.032

2.965 ± 0.04

11.827 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.02

2.953 ± 0.022

4.761 ± 0.03

4.962 ± 0.04

6.684 ± 0.034

5.783 ± 0.028

4.718 ± 0.026

6.973 ± 0.035

1.441 ± 0.017

2.518 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski