Aurantimicrobium minutum
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1574 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A173LXB8|A0A173LXB8_9MICO Uncharacterized protein OS=Aurantimicrobium minutum OX=708131 GN=AUMI_110630 PE=4 SV=1
MM1 pKa = 7.79 ADD3 pKa = 3.1 STQTYY8 pKa = 9.77 LQLLDD13 pKa = 4.42 AVQAHH18 pKa = 6.34 IQAITGEE25 pKa = 4.15 DD26 pKa = 3.48 TIARR30 pKa = 11.84 DD31 pKa = 3.24 WVLVCGVTDD40 pKa = 3.96 INAPANDD47 pKa = 3.43 IDD49 pKa = 4.11 ADD51 pKa = 3.51 IRR53 pKa = 11.84 IDD55 pKa = 3.57 YY56 pKa = 10.6 SPRR59 pKa = 11.84 TTTYY63 pKa = 10.18 TLNGLLNLALDD74 pKa = 4.05 VFQPEE79 pKa = 4.45 LEE81 pKa = 4.14
Molecular weight: 8.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.694
IPC_protein 3.643
Toseland 3.427
ProMoST 3.808
Dawson 3.668
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.478
Grimsley 3.338
Solomon 3.63
Lehninger 3.592
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.77
Patrickios 1.875
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A173LYI0|A0A173LYI0_9MICO Uncharacterized protein OS=Aurantimicrobium minutum OX=708131 GN=AUMI_113560 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.45 VHH17 pKa = 5.31 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.65 GRR40 pKa = 11.84 SEE42 pKa = 3.94 LSAA45 pKa = 4.73
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.453
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.735
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1574
0
1574
503936
39
3103
320.2
34.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.305 ± 0.085
0.535 ± 0.014
5.486 ± 0.054
6.259 ± 0.072
3.538 ± 0.037
8.2 ± 0.063
1.974 ± 0.035
5.718 ± 0.045
3.7 ± 0.052
9.826 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.087 ± 0.031
3.131 ± 0.039
4.826 ± 0.037
3.267 ± 0.035
5.415 ± 0.06
6.408 ± 0.083
6.202 ± 0.089
8.503 ± 0.057
1.36 ± 0.027
2.261 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here