Anthoxanthum odoratum amalgavirus 1
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V0A0Q4|A0A2V0A0Q4_9VIRU RNA-dependent RNA polymerase OS=Anthoxanthum odoratum amalgavirus 1 OX=2065797 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.78 AEE3 pKa = 4.05 PRR5 pKa = 11.84 RR6 pKa = 11.84 TFQTAAPTEE15 pKa = 4.09 GDD17 pKa = 3.75 YY18 pKa = 11.05 VANLPADD25 pKa = 3.71 EE26 pKa = 5.1 DD27 pKa = 3.96 AAFVYY32 pKa = 8.93 FARR35 pKa = 11.84 WVFTTYY41 pKa = 10.76 HH42 pKa = 6.61 LAAGLLDD49 pKa = 3.97 PATYY53 pKa = 10.12 RR54 pKa = 11.84 PEE56 pKa = 4.71 GYY58 pKa = 9.63 SDD60 pKa = 3.77 KK61 pKa = 11.68 DD62 pKa = 3.01 MAARR66 pKa = 11.84 LRR68 pKa = 11.84 FFKK71 pKa = 10.8 GKK73 pKa = 9.4 EE74 pKa = 3.55 ADD76 pKa = 3.78 VIDD79 pKa = 4.31 TIFAVGVRR87 pKa = 11.84 KK88 pKa = 10.29 SFFTAKK94 pKa = 10.1 EE95 pKa = 3.98 SATFEE100 pKa = 3.89 QFANFLEE107 pKa = 4.37 FLRR110 pKa = 11.84 TADD113 pKa = 3.01 GAAAVTEE120 pKa = 4.11 DD121 pKa = 3.51 LRR123 pKa = 11.84 RR124 pKa = 11.84 SRR126 pKa = 11.84 FQAAGKK132 pKa = 10.22 GVFNAVEE139 pKa = 4.11 ISSVAQLTVQLADD152 pKa = 4.05 LEE154 pKa = 4.48 LHH156 pKa = 5.46 KK157 pKa = 10.52 HH158 pKa = 5.18 RR159 pKa = 11.84 MSDD162 pKa = 3.25 ASQKK166 pKa = 10.8 RR167 pKa = 11.84 IDD169 pKa = 3.98 EE170 pKa = 4.52 LEE172 pKa = 3.9 LLIAKK177 pKa = 9.17 EE178 pKa = 4.13 KK179 pKa = 10.87 EE180 pKa = 4.11 SLEE183 pKa = 4.5 TKK185 pKa = 10.57 LKK187 pKa = 10.42 MSDD190 pKa = 3.57 EE191 pKa = 4.4 EE192 pKa = 4.4 FFPASIYY199 pKa = 10.84 KK200 pKa = 10.18 KK201 pKa = 9.96 LNPMKK206 pKa = 10.13 LQKK209 pKa = 10.2 EE210 pKa = 4.24 CWVRR214 pKa = 11.84 AKK216 pKa = 10.81 ASSTEE221 pKa = 3.93 LAAVAVPTNDD231 pKa = 3.4 QLADD235 pKa = 3.28 ALKK238 pKa = 9.83 NFKK241 pKa = 10.83 EE242 pKa = 4.31 EE243 pKa = 3.8 VEE245 pKa = 4.14 RR246 pKa = 11.84 RR247 pKa = 11.84 HH248 pKa = 6.53 AVDD251 pKa = 6.31 FLAQQNRR258 pKa = 11.84 LTALRR263 pKa = 11.84 EE264 pKa = 4.01 YY265 pKa = 10.68 VNKK268 pKa = 10.54 KK269 pKa = 9.35 ILSFRR274 pKa = 11.84 ARR276 pKa = 11.84 GKK278 pKa = 10.32 YY279 pKa = 9.87 RR280 pKa = 11.84 IATRR284 pKa = 11.84 LGHH287 pKa = 6.24 VLAAMDD293 pKa = 4.1 GQASGEE299 pKa = 4.17 VPPAGAHH306 pKa = 6.42 IGDD309 pKa = 4.59 GDD311 pKa = 4.05 DD312 pKa = 5.33 SDD314 pKa = 5.43 GEE316 pKa = 4.62 DD317 pKa = 4.07 YY318 pKa = 11.08 IPDD321 pKa = 3.78 YY322 pKa = 10.98 EE323 pKa = 5.09 RR324 pKa = 11.84 EE325 pKa = 4.1 LSDD328 pKa = 3.48 EE329 pKa = 4.24 SSGSDD334 pKa = 3.65 EE335 pKa = 4.37 EE336 pKa = 4.6 SGSAGGTPEE345 pKa = 3.9 RR346 pKa = 11.84 HH347 pKa = 5.36 RR348 pKa = 11.84 TPTPARR354 pKa = 11.84 SRR356 pKa = 11.84 RR357 pKa = 11.84 PRR359 pKa = 11.84 RR360 pKa = 11.84 RR361 pKa = 11.84 RR362 pKa = 11.84 DD363 pKa = 3.25 YY364 pKa = 10.44 TGLARR369 pKa = 11.84 GSAPARR375 pKa = 11.84 NTRR378 pKa = 11.84 SRR380 pKa = 11.84 KK381 pKa = 9.27 AA382 pKa = 3.17
Molecular weight: 42.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.64
IPC2_protein 6.62
IPC_protein 6.985
Toseland 7.249
ProMoST 7.263
Dawson 7.41
Bjellqvist 7.088
Wikipedia 7.41
Rodwell 7.366
Grimsley 7.337
Solomon 7.556
Lehninger 7.541
Nozaki 7.41
DTASelect 7.614
Thurlkill 7.644
EMBOSS 7.775
Sillero 7.673
Patrickios 4.8
IPC_peptide 7.556
IPC2_peptide 7.0
IPC2.peptide.svr19 7.113
Protein with the highest isoelectric point:
>tr|A0A2V0A0Q4|A0A2V0A0Q4_9VIRU RNA-dependent RNA polymerase OS=Anthoxanthum odoratum amalgavirus 1 OX=2065797 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.78 AEE3 pKa = 4.05 PRR5 pKa = 11.84 RR6 pKa = 11.84 TFQTAAPTEE15 pKa = 4.09 GDD17 pKa = 3.75 YY18 pKa = 11.05 VANLPADD25 pKa = 3.71 EE26 pKa = 5.1 DD27 pKa = 3.96 AAFVYY32 pKa = 8.93 FARR35 pKa = 11.84 WVFTTYY41 pKa = 10.76 HH42 pKa = 6.61 LAAGLLDD49 pKa = 3.97 PATYY53 pKa = 10.12 RR54 pKa = 11.84 PEE56 pKa = 4.71 GYY58 pKa = 9.63 SDD60 pKa = 3.77 KK61 pKa = 11.68 DD62 pKa = 3.01 MAARR66 pKa = 11.84 LRR68 pKa = 11.84 FFKK71 pKa = 10.8 GKK73 pKa = 9.4 EE74 pKa = 3.55 ADD76 pKa = 3.78 VIDD79 pKa = 4.31 TIFAVGVRR87 pKa = 11.84 KK88 pKa = 10.29 SFFTAKK94 pKa = 10.1 EE95 pKa = 3.98 SATFEE100 pKa = 3.89 QFANFLEE107 pKa = 4.37 FLRR110 pKa = 11.84 TADD113 pKa = 3.01 GAAAVTEE120 pKa = 4.11 DD121 pKa = 3.51 LRR123 pKa = 11.84 RR124 pKa = 11.84 SRR126 pKa = 11.84 FQAAGKK132 pKa = 10.22 GVFNAVEE139 pKa = 4.11 ISSVAQLTVQLADD152 pKa = 4.05 LEE154 pKa = 4.48 LHH156 pKa = 5.46 KK157 pKa = 10.52 HH158 pKa = 5.18 RR159 pKa = 11.84 MSDD162 pKa = 3.25 ASQKK166 pKa = 10.8 RR167 pKa = 11.84 IDD169 pKa = 3.98 EE170 pKa = 4.52 LEE172 pKa = 3.9 LLIAKK177 pKa = 9.17 EE178 pKa = 4.13 KK179 pKa = 10.87 EE180 pKa = 4.11 SLEE183 pKa = 4.5 TKK185 pKa = 10.57 LKK187 pKa = 10.42 MSDD190 pKa = 3.57 EE191 pKa = 4.4 EE192 pKa = 4.4 FFPASIYY199 pKa = 10.84 KK200 pKa = 10.18 KK201 pKa = 9.96 LNPMKK206 pKa = 10.13 LQKK209 pKa = 10.2 EE210 pKa = 4.24 CWVRR214 pKa = 11.84 AKK216 pKa = 10.81 ASSTEE221 pKa = 3.93 LAAVAVPTNDD231 pKa = 3.4 QLADD235 pKa = 3.28 ALKK238 pKa = 9.83 NFKK241 pKa = 10.83 EE242 pKa = 4.31 EE243 pKa = 3.8 VEE245 pKa = 4.14 RR246 pKa = 11.84 RR247 pKa = 11.84 HH248 pKa = 6.53 AVDD251 pKa = 6.31 FLAQQNRR258 pKa = 11.84 LTALRR263 pKa = 11.84 EE264 pKa = 4.01 YY265 pKa = 10.68 VNKK268 pKa = 10.52 KK269 pKa = 9.13 ILSFVLVEE277 pKa = 4.29 NTGSRR282 pKa = 11.84 PALDD286 pKa = 3.98 TSWLPWTAKK295 pKa = 10.54 LLEE298 pKa = 4.85 KK299 pKa = 10.89 YY300 pKa = 9.01 PLPEE304 pKa = 5.16 RR305 pKa = 11.84 ISATAMIPMGRR316 pKa = 11.84 TTSPTTSVSCRR327 pKa = 11.84 TNLLEE332 pKa = 4.54 VMRR335 pKa = 11.84 SPALLAVRR343 pKa = 11.84 QRR345 pKa = 11.84 GTGPPPRR352 pKa = 11.84 LGAGVPAAVGTTPVLQGGARR372 pKa = 11.84 LHH374 pKa = 6.23 VIRR377 pKa = 11.84 APVRR381 pKa = 11.84 PDD383 pKa = 2.86 RR384 pKa = 11.84 RR385 pKa = 11.84 GIPYY389 pKa = 10.3 ARR391 pKa = 11.84 SKK393 pKa = 10.32 WEE395 pKa = 3.79 AGVRR399 pKa = 11.84 KK400 pKa = 9.86 IIGGGEE406 pKa = 3.87 LSDD409 pKa = 3.45 WQAASSKK416 pKa = 9.72 VRR418 pKa = 11.84 CGGNASDD425 pKa = 5.64 ALLLLADD432 pKa = 4.48 ASDD435 pKa = 3.86 RR436 pKa = 11.84 LPGKK440 pKa = 6.8 TLRR443 pKa = 11.84 GLFTLRR449 pKa = 11.84 GARR452 pKa = 11.84 DD453 pKa = 3.66 ALRR456 pKa = 11.84 LPSGLSVPDD465 pKa = 4.25 GRR467 pKa = 11.84 ACCNIKK473 pKa = 10.27 HH474 pKa = 6.09 FNVEE478 pKa = 3.64 ATAGPFLRR486 pKa = 11.84 AFGVKK491 pKa = 9.8 KK492 pKa = 10.6 KK493 pKa = 10.76 EE494 pKa = 4.09 SLEE497 pKa = 3.83 QLLGDD502 pKa = 4.22 FVWEE506 pKa = 4.52 CFDD509 pKa = 6.04 DD510 pKa = 4.87 FATNDD515 pKa = 2.99 ADD517 pKa = 3.83 IRR519 pKa = 11.84 RR520 pKa = 11.84 LPFFGMRR527 pKa = 11.84 IGFRR531 pKa = 11.84 TKK533 pKa = 10.36 LLKK536 pKa = 10.35 KK537 pKa = 10.41 SEE539 pKa = 4.32 MLTKK543 pKa = 10.15 IRR545 pKa = 11.84 DD546 pKa = 3.9 FKK548 pKa = 11.17 PLGRR552 pKa = 11.84 CVMMLDD558 pKa = 5.12 AIEE561 pKa = 4.41 QFCSSPLYY569 pKa = 10.56 NVLSKK574 pKa = 10.97 LSADD578 pKa = 3.55 ALKK581 pKa = 11.07 DD582 pKa = 3.45 PMSGFRR588 pKa = 11.84 NTAVRR593 pKa = 11.84 ASSDD597 pKa = 2.64 WSYY600 pKa = 9.73 MWEE603 pKa = 4.09 EE604 pKa = 3.54 IKK606 pKa = 10.33 EE607 pKa = 4.24 AKK609 pKa = 10.17 VCMEE613 pKa = 4.68 LDD615 pKa = 2.81 WSKK618 pKa = 10.93 FDD620 pKa = 4.73 RR621 pKa = 11.84 EE622 pKa = 4.11 RR623 pKa = 11.84 PRR625 pKa = 11.84 CDD627 pKa = 3.33 LEE629 pKa = 4.55 FMVDD633 pKa = 3.59 VVISCFAPKK642 pKa = 10.29 NEE644 pKa = 3.88 RR645 pKa = 11.84 EE646 pKa = 3.86 QRR648 pKa = 11.84 LLRR651 pKa = 11.84 GYY653 pKa = 6.91 EE654 pKa = 3.63 VCMRR658 pKa = 11.84 RR659 pKa = 11.84 AIVEE663 pKa = 4.18 RR664 pKa = 11.84 VALLDD669 pKa = 4.29 DD670 pKa = 4.4 GALFEE675 pKa = 5.2 IDD677 pKa = 3.61 GMVPSGSLWTGWLDD691 pKa = 3.31 TALNILYY698 pKa = 10.4 LSAALSEE705 pKa = 4.86 AGFSSYY711 pKa = 7.45 MARR714 pKa = 11.84 PKK716 pKa = 10.74 CAGDD720 pKa = 3.9 DD721 pKa = 3.7 NLTLFMEE728 pKa = 4.81 DD729 pKa = 3.42 VPDD732 pKa = 3.63 GRR734 pKa = 11.84 LIQVRR739 pKa = 11.84 GLLNDD744 pKa = 3.6 WFLAGIKK751 pKa = 10.57 DD752 pKa = 3.58 EE753 pKa = 5.12 DD754 pKa = 3.99 FAITRR759 pKa = 11.84 APFHH763 pKa = 6.8 VEE765 pKa = 4.08 TYY767 pKa = 10.35 QAVFPPGTDD776 pKa = 3.29 LTKK779 pKa = 10.06 GTSKK783 pKa = 10.63 IIDD786 pKa = 3.18 QCEE789 pKa = 3.68 WVRR792 pKa = 11.84 FEE794 pKa = 4.82 GQLQINQEE802 pKa = 4.01 QGLSHH807 pKa = 6.82 RR808 pKa = 11.84 WQYY811 pKa = 10.66 RR812 pKa = 11.84 FKK814 pKa = 10.9 GKK816 pKa = 9.57 PKK818 pKa = 10.28 FLSCYY823 pKa = 8.28 WLADD827 pKa = 3.34 GRR829 pKa = 11.84 PIRR832 pKa = 11.84 PASDD836 pKa = 3.13 NVEE839 pKa = 3.83 KK840 pKa = 10.78 LLYY843 pKa = 10.0 PEE845 pKa = 5.91 GIHH848 pKa = 7.08 KK849 pKa = 10.59 SVEE852 pKa = 4.29 DD853 pKa = 4.09 YY854 pKa = 11.09 ISACLAMVVDD864 pKa = 4.82 NPFNQHH870 pKa = 5.29 NVNHH874 pKa = 6.16 MKK876 pKa = 10.09 HH877 pKa = 6.55 RR878 pKa = 11.84 YY879 pKa = 9.24 LIAQQIKK886 pKa = 9.37 RR887 pKa = 11.84 ILVTGIPDD895 pKa = 3.77 RR896 pKa = 11.84 LVLALARR903 pKa = 11.84 IRR905 pKa = 11.84 PDD907 pKa = 3.29 GEE909 pKa = 3.6 EE910 pKa = 4.66 DD911 pKa = 2.97 IPYY914 pKa = 10.64 PMIAPWRR921 pKa = 11.84 RR922 pKa = 11.84 FKK924 pKa = 10.93 EE925 pKa = 4.18 YY926 pKa = 10.19 IDD928 pKa = 4.17 LDD930 pKa = 3.74 SYY932 pKa = 11.77 EE933 pKa = 5.42 PVQQWIKK940 pKa = 10.9 EE941 pKa = 3.92 FDD943 pKa = 3.78 DD944 pKa = 4.77 FVAGISGLYY953 pKa = 9.33 VRR955 pKa = 11.84 STTGGIDD962 pKa = 2.88 AYY964 pKa = 10.26 KK965 pKa = 10.72 FMDD968 pKa = 5.57 FIRR971 pKa = 11.84 GDD973 pKa = 3.5 AVIGEE978 pKa = 4.5 GQWGNEE984 pKa = 3.45 MDD986 pKa = 2.9 RR987 pKa = 11.84 WIRR990 pKa = 11.84 FITEE994 pKa = 4.03 HH995 pKa = 5.98 PVSRR999 pKa = 11.84 SLRR1002 pKa = 11.84 KK1003 pKa = 9.53 ARR1005 pKa = 11.84 RR1006 pKa = 11.84 HH1007 pKa = 5.56 NPASVPSAQVNQYY1020 pKa = 7.03 YY1021 pKa = 9.79 QRR1023 pKa = 11.84 AAEE1026 pKa = 4.06 GLNVYY1031 pKa = 9.69 RR1032 pKa = 11.84 QQLVNHH1038 pKa = 6.83 RR1039 pKa = 11.84 LDD1041 pKa = 3.01 SSRR1044 pKa = 11.84 EE1045 pKa = 3.72 YY1046 pKa = 11.1 GLWVSDD1052 pKa = 3.43 LLRR1055 pKa = 11.84 RR1056 pKa = 3.93
Molecular weight: 119.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.228
IPC2_protein 7.702
IPC_protein 7.512
Toseland 7.19
ProMoST 8.185
Dawson 8.273
Bjellqvist 8.609
Wikipedia 8.2
Rodwell 8.317
Grimsley 7.22
Solomon 8.317
Lehninger 8.331
Nozaki 8.799
DTASelect 8.346
Thurlkill 8.419
EMBOSS 8.478
Sillero 8.726
Patrickios 4.647
IPC_peptide 8.317
IPC2_peptide 7.454
IPC2.peptide.svr19 7.715
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1438
382
1056
719.0
81.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.848 ± 1.491
1.182 ± 0.454
6.676 ± 0.193
6.885 ± 0.735
5.076 ± 0.078
6.05 ± 0.143
1.53 ± 0.149
3.964 ± 0.663
5.563 ± 0.226
9.458 ± 0.791
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.156 ± 0.288
2.782 ± 0.21
4.798 ± 0.301
3.199 ± 0.157
8.623 ± 0.395
6.12 ± 0.209
4.798 ± 0.473
5.911 ± 0.462
1.599 ± 0.53
2.782 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here