Streptococcus satellite phage Javan397

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZM92|A0A4D5ZM92_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan397 OX=2558677 GN=JavanS397_0012 PE=4 SV=1
MM1 pKa = 7.44IRR3 pKa = 11.84NNLAKK8 pKa = 10.79LMIDD12 pKa = 3.64RR13 pKa = 11.84GISATQLYY21 pKa = 9.73NDD23 pKa = 3.75TGIARR28 pKa = 11.84STISKK33 pKa = 9.86ISNNNTDD40 pKa = 4.38KK41 pKa = 11.09ISLQTIDD48 pKa = 4.92KK49 pKa = 8.86ICNYY53 pKa = 10.13LGVSPMQFFDD63 pKa = 3.69FLSYY67 pKa = 9.93EE68 pKa = 4.39VKK70 pKa = 10.63VSCGFDD76 pKa = 3.14DD77 pKa = 4.55YY78 pKa = 11.52EE79 pKa = 4.51TFEE82 pKa = 4.46EE83 pKa = 4.49MIINYY88 pKa = 9.42KK89 pKa = 10.23DD90 pKa = 3.07NSYY93 pKa = 10.67IKK95 pKa = 9.94EE96 pKa = 4.16KK97 pKa = 10.35AWLSISFYY105 pKa = 11.44SMNILKK111 pKa = 10.75YY112 pKa = 10.68NFDD115 pKa = 3.5FDD117 pKa = 4.54FYY119 pKa = 10.92YY120 pKa = 10.4INDD123 pKa = 3.75FEE125 pKa = 4.49EE126 pKa = 5.95GYY128 pKa = 9.94PFDD131 pKa = 5.41DD132 pKa = 5.34GYY134 pKa = 10.66IAYY137 pKa = 9.32LINMEE142 pKa = 4.29YY143 pKa = 10.57SSKK146 pKa = 10.89NPSAEE151 pKa = 3.67IFNQIPIQFKK161 pKa = 10.36NDD163 pKa = 3.27IVNMAKK169 pKa = 10.54DD170 pKa = 3.42SLSEE174 pKa = 3.72FFNVSKK180 pKa = 10.81EE181 pKa = 3.93SATIKK186 pKa = 10.7EE187 pKa = 4.17FDD189 pKa = 3.64PEE191 pKa = 4.03FLEE194 pKa = 4.9FLL196 pKa = 4.13

Molecular weight: 22.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZMK4|A0A4D5ZMK4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan397 OX=2558677 GN=JavanS397_0018 PE=4 SV=1
MM1 pKa = 7.81EE2 pKa = 4.55YY3 pKa = 10.36SISHH7 pKa = 5.81PAEE10 pKa = 3.69RR11 pKa = 11.84QRR13 pKa = 11.84IRR15 pKa = 11.84GIGKK19 pKa = 9.45EE20 pKa = 3.72KK21 pKa = 10.55DD22 pKa = 3.12RR23 pKa = 11.84RR24 pKa = 11.84PTLDD28 pKa = 3.15QIKK31 pKa = 9.98EE32 pKa = 3.96RR33 pKa = 11.84QQLKK37 pKa = 10.2KK38 pKa = 10.63LKK40 pKa = 10.11KK41 pKa = 9.17KK42 pKa = 10.31RR43 pKa = 11.84NRR45 pKa = 11.84GKK47 pKa = 10.6

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

3135

38

493

174.2

20.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.402 ± 0.266

0.734 ± 0.118

6.316 ± 0.459

7.049 ± 0.389

4.848 ± 0.523

4.306 ± 0.481

1.563 ± 0.226

8.804 ± 0.632

8.804 ± 0.447

10.654 ± 0.641

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.914 ± 0.219

6.411 ± 0.355

3.158 ± 0.505

3.923 ± 0.367

4.147 ± 0.523

6.124 ± 0.482

5.869 ± 0.608

5.008 ± 0.489

0.957 ± 0.219

5.008 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski