Staphylococcus phage Sebago

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; Staphylococcus virus Sebago

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MIA3|A0A482MIA3_9CAUD Uncharacterized protein OS=Staphylococcus phage Sebago OX=2557555 GN=CPT_Sebago_051 PE=4 SV=1
MM1 pKa = 7.51NNRR4 pKa = 11.84EE5 pKa = 4.1QIEE8 pKa = 3.98QSVISASAYY17 pKa = 9.87NGNDD21 pKa = 3.18TEE23 pKa = 4.28EE24 pKa = 4.07LLKK27 pKa = 10.81EE28 pKa = 4.0IEE30 pKa = 4.3DD31 pKa = 4.23VYY33 pKa = 11.57KK34 pKa = 10.64KK35 pKa = 10.72AQAFDD40 pKa = 4.66EE41 pKa = 4.34ILEE44 pKa = 4.2GLPNAMQDD52 pKa = 3.36ALKK55 pKa = 10.66EE56 pKa = 4.16DD57 pKa = 3.68IYY59 pKa = 11.25LDD61 pKa = 3.66EE62 pKa = 5.14AVGIMTSQVVYY73 pKa = 10.27KK74 pKa = 10.8YY75 pKa = 10.77EE76 pKa = 3.89EE77 pKa = 4.06EE78 pKa = 4.28QEE80 pKa = 3.93NDD82 pKa = 2.92

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MI22|A0A482MI22_9CAUD AAA-ATPase OS=Staphylococcus phage Sebago OX=2557555 GN=CPT_Sebago_058 PE=4 SV=1
MM1 pKa = 7.57SIVKK5 pKa = 10.22INGKK9 pKa = 8.42PYY11 pKa = 10.87KK12 pKa = 9.54FTEE15 pKa = 4.42HH16 pKa = 6.09EE17 pKa = 4.0NEE19 pKa = 4.33LIKK22 pKa = 11.15KK23 pKa = 9.98NGLTPGMVAKK33 pKa = 10.32RR34 pKa = 11.84VRR36 pKa = 11.84GGWALLEE43 pKa = 4.48ALHH46 pKa = 6.68APYY49 pKa = 11.09GMRR52 pKa = 11.84LAEE55 pKa = 4.07YY56 pKa = 10.6KK57 pKa = 10.62EE58 pKa = 3.92IVLAKK63 pKa = 10.18IMEE66 pKa = 4.51RR67 pKa = 11.84EE68 pKa = 4.12SKK70 pKa = 9.36EE71 pKa = 4.1RR72 pKa = 11.84EE73 pKa = 3.7MARR76 pKa = 11.84QRR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 9.17EE81 pKa = 3.73AEE83 pKa = 3.86LRR85 pKa = 11.84KK86 pKa = 10.04KK87 pKa = 10.35KK88 pKa = 9.92PHH90 pKa = 6.47LFNVPQKK97 pKa = 10.53HH98 pKa = 6.08SRR100 pKa = 11.84DD101 pKa = 3.97PYY103 pKa = 10.08WFDD106 pKa = 3.01VTYY109 pKa = 11.33NQMFKK114 pKa = 10.23KK115 pKa = 9.86WSEE118 pKa = 3.76AA119 pKa = 3.32

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

14046

30

961

200.7

22.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.61 ± 0.406

0.47 ± 0.077

6.685 ± 0.285

7.255 ± 0.428

4.378 ± 0.198

5.938 ± 0.306

1.687 ± 0.148

7.347 ± 0.267

9.056 ± 0.372

7.661 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.185

6.443 ± 0.242

2.762 ± 0.213

4.001 ± 0.269

4.065 ± 0.221

5.987 ± 0.236

6.208 ± 0.293

6.052 ± 0.252

1.203 ± 0.182

4.606 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski