Amino acid dipepetide frequency for Raineyella sp. CBA3103

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.383AlaAla: 18.383 ± 0.208	0.991AlaCys: 0.991 ± 0.03	8.047AlaAsp: 8.047 ± 0.107	7.742AlaGlu: 7.742 ± 0.116	3.412AlaPhe: 3.412 ± 0.069	12.274AlaGly: 12.274 ± 0.138	2.573AlaHis: 2.573 ± 0.058	4.791AlaIle: 4.791 ± 0.084	2.482AlaLys: 2.482 ± 0.063	13.165AlaLeu: 13.165 ± 0.167	3.077AlaMet: 3.077 ± 0.058	2.02AlaAsn: 2.02 ± 0.048	6.187AlaPro: 6.187 ± 0.112	3.741AlaGln: 3.741 ± 0.064	9.116AlaArg: 9.116 ± 0.127	6.175AlaSer: 6.175 ± 0.081	7.797AlaThr: 7.797 ± 0.105	11.059AlaVal: 11.059 ± 0.128	2.036AlaTrp: 2.036 ± 0.046	2.828AlaTyr: 2.828 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.819CysAla: 0.819 ± 0.027	0.12CysCys: 0.12 ± 0.012	0.495CysAsp: 0.495 ± 0.022	0.346CysGlu: 0.346 ± 0.021	0.207CysPhe: 0.207 ± 0.016	0.903CysGly: 0.903 ± 0.031	0.215CysHis: 0.215 ± 0.014	0.247CysIle: 0.247 ± 0.018	0.11CysLys: 0.11 ± 0.012	0.666CysLeu: 0.666 ± 0.028	0.116CysMet: 0.116 ± 0.013	0.13CysAsn: 0.13 ± 0.014	0.529CysPro: 0.529 ± 0.024	0.199CysGln: 0.199 ± 0.016	0.632CysArg: 0.632 ± 0.028	0.455CysSer: 0.455 ± 0.024	0.482CysThr: 0.482 ± 0.021	0.506CysVal: 0.506 ± 0.022	0.121CysTrp: 0.121 ± 0.011	0.143CysTyr: 0.143 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.18AspAla: 7.18 ± 0.096	0.336AspCys: 0.336 ± 0.017	3.704AspAsp: 3.704 ± 0.068	3.941AspGlu: 3.941 ± 0.076	1.675AspPhe: 1.675 ± 0.045	5.362AspGly: 5.362 ± 0.089	1.52AspHis: 1.52 ± 0.041	2.264AspIle: 2.264 ± 0.05	1.142AspLys: 1.142 ± 0.038	6.986AspLeu: 6.986 ± 0.095	1.049AspMet: 1.049 ± 0.036	0.994AspAsn: 0.994 ± 0.037	4.767AspPro: 4.767 ± 0.069	1.931AspGln: 1.931 ± 0.05	5.012AspArg: 5.012 ± 0.077	2.353AspSer: 2.353 ± 0.055	3.017AspThr: 3.017 ± 0.063	5.221AspVal: 5.221 ± 0.087	0.975AspTrp: 0.975 ± 0.033	1.226AspTyr: 1.226 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	7.24GluAla: 7.24 ± 0.1	0.309GluCys: 0.309 ± 0.019	3.018GluAsp: 3.018 ± 0.074	3.28GluGlu: 3.28 ± 0.057	1.466GluPhe: 1.466 ± 0.039	4.286GluGly: 4.286 ± 0.066	1.455GluHis: 1.455 ± 0.041	2.391GluIle: 2.391 ± 0.053	1.212GluLys: 1.212 ± 0.036	5.545GluLeu: 5.545 ± 0.087	1.065GluMet: 1.065 ± 0.031	0.988GluAsn: 0.988 ± 0.036	2.726GluPro: 2.726 ± 0.056	2.135GluGln: 2.135 ± 0.054	4.747GluArg: 4.747 ± 0.081	2.386GluSer: 2.386 ± 0.053	2.61GluThr: 2.61 ± 0.049	4.944GluVal: 4.944 ± 0.074	0.744GluTrp: 0.744 ± 0.034	1.012GluTyr: 1.012 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.388PheAla: 3.388 ± 0.065	0.276PheCys: 0.276 ± 0.018	2.026PheAsp: 2.026 ± 0.053	1.394PheGlu: 1.394 ± 0.041	0.945PhePhe: 0.945 ± 0.04	3.213PheGly: 3.213 ± 0.066	0.642PheHis: 0.642 ± 0.026	1.004PheIle: 1.004 ± 0.036	0.412PheLys: 0.412 ± 0.02	2.786PheLeu: 2.786 ± 0.065	0.505PheMet: 0.505 ± 0.025	0.598PheAsn: 0.598 ± 0.027	1.36PhePro: 1.36 ± 0.04	0.675PheGln: 0.675 ± 0.027	1.811PheArg: 1.811 ± 0.045	1.617PheSer: 1.617 ± 0.05	1.991PheThr: 1.991 ± 0.052	2.481PheVal: 2.481 ± 0.053	0.437PheTrp: 0.437 ± 0.024	0.645PheTyr: 0.645 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.179GlyAla: 10.179 ± 0.13	0.862GlyCys: 0.862 ± 0.033	4.911GlyAsp: 4.911 ± 0.085	4.542GlyGlu: 4.542 ± 0.069	3.002GlyPhe: 3.002 ± 0.068	7.876GlyGly: 7.876 ± 0.116	2.233GlyHis: 2.233 ± 0.055	4.203GlyIle: 4.203 ± 0.075	2.049GlyLys: 2.049 ± 0.06	9.858GlyLeu: 9.858 ± 0.121	2.207GlyMet: 2.207 ± 0.051	1.663GlyAsn: 1.663 ± 0.048	4.84GlyPro: 4.84 ± 0.075	3.048GlyGln: 3.048 ± 0.061	7.388GlyArg: 7.388 ± 0.092	5.345GlySer: 5.345 ± 0.088	5.951GlyThr: 5.951 ± 0.101	7.932GlyVal: 7.932 ± 0.115	1.81GlyTrp: 1.81 ± 0.045	2.212GlyTyr: 2.212 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	2.326HisAla: 2.326 ± 0.057	0.199HisCys: 0.199 ± 0.015	1.422HisAsp: 1.422 ± 0.041	1.228HisGlu: 1.228 ± 0.042	0.633HisPhe: 0.633 ± 0.025	2.119HisGly: 2.119 ± 0.049	0.717HisHis: 0.717 ± 0.027	0.669HisIle: 0.669 ± 0.028	0.32HisLys: 0.32 ± 0.019	2.514HisLeu: 2.514 ± 0.052	0.376HisMet: 0.376 ± 0.019	0.416HisAsn: 0.416 ± 0.021	1.814HisPro: 1.814 ± 0.049	0.723HisGln: 0.723 ± 0.026	2.031HisArg: 2.031 ± 0.047	0.984HisSer: 0.984 ± 0.032	1.131HisThr: 1.131 ± 0.037	1.888HisVal: 1.888 ± 0.047	0.39HisTrp: 0.39 ± 0.021	0.471HisTyr: 0.471 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.71IleAla: 5.71 ± 0.086	0.344IleCys: 0.344 ± 0.019	2.854IleAsp: 2.854 ± 0.061	2.213IleGlu: 2.213 ± 0.05	1.033IlePhe: 1.033 ± 0.037	4.413IleGly: 4.413 ± 0.082	0.797IleHis: 0.797 ± 0.027	1.608IleIle: 1.608 ± 0.048	0.759IleLys: 0.759 ± 0.026	3.373IleLeu: 3.373 ± 0.072	0.645IleMet: 0.645 ± 0.029	0.931IleAsn: 0.931 ± 0.033	2.166IlePro: 2.166 ± 0.052	0.895IleGln: 0.895 ± 0.031	2.793IleArg: 2.793 ± 0.05	2.142IleSer: 2.142 ± 0.053	2.687IleThr: 2.687 ± 0.054	3.61IleVal: 3.61 ± 0.057	0.452IleTrp: 0.452 ± 0.019	0.674IleTyr: 0.674 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	2.545LysAla: 2.545 ± 0.055	0.088LysCys: 0.088 ± 0.009	1.206LysAsp: 1.206 ± 0.034	1.037LysGlu: 1.037 ± 0.033	0.473LysPhe: 0.473 ± 0.022	1.649LysGly: 1.649 ± 0.053	0.396LysHis: 0.396 ± 0.024	0.847LysIle: 0.847 ± 0.034	0.722LysLys: 0.722 ± 0.036	1.727LysLeu: 1.727 ± 0.045	0.392LysMet: 0.392 ± 0.021	0.467LysAsn: 0.467 ± 0.025	1.11LysPro: 1.11 ± 0.039	0.673LysGln: 0.673 ± 0.025	1.288LysArg: 1.288 ± 0.039	0.991LysSer: 0.991 ± 0.032	1.023LysThr: 1.023 ± 0.038	1.851LysVal: 1.851 ± 0.058	0.221LysTrp: 0.221 ± 0.017	0.478LysTyr: 0.478 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	14.647LeuAla: 14.647 ± 0.142	0.668LeuCys: 0.668 ± 0.026	6.612LeuAsp: 6.612 ± 0.082	5.15LeuGlu: 5.15 ± 0.082	2.6LeuPhe: 2.6 ± 0.055	9.541LeuGly: 9.541 ± 0.131	2.07LeuHis: 2.07 ± 0.044	3.643LeuIle: 3.643 ± 0.067	1.683LeuLys: 1.683 ± 0.05	10.194LeuLeu: 10.194 ± 0.152	1.97LeuMet: 1.97 ± 0.048	1.811LeuAsn: 1.811 ± 0.047	5.941LeuPro: 5.941 ± 0.086	2.582LeuGln: 2.582 ± 0.049	7.562LeuArg: 7.562 ± 0.094	5.568LeuSer: 5.568 ± 0.084	6.524LeuThr: 6.524 ± 0.105	10.001LeuVal: 10.001 ± 0.108	1.29LeuTrp: 1.29 ± 0.048	1.717LeuTyr: 1.717 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.814MetAla: 2.814 ± 0.049	0.13MetCys: 0.13 ± 0.01	1.013MetAsp: 1.013 ± 0.032	0.843MetGlu: 0.843 ± 0.032	0.587MetPhe: 0.587 ± 0.024	1.651MetGly: 1.651 ± 0.045	0.38MetHis: 0.38 ± 0.02	0.952MetIle: 0.952 ± 0.032	0.528MetLys: 0.528 ± 0.023	1.976MetLeu: 1.976 ± 0.048	0.452MetMet: 0.452 ± 0.024	0.515MetAsn: 0.515 ± 0.022	1.208MetPro: 1.208 ± 0.036	0.534MetGln: 0.534 ± 0.026	1.397MetArg: 1.397 ± 0.039	1.526MetSer: 1.526 ± 0.039	1.91MetThr: 1.91 ± 0.043	1.864MetVal: 1.864 ± 0.05	0.246MetTrp: 0.246 ± 0.02	0.344MetTyr: 0.344 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.291AsnAla: 2.291 ± 0.05	0.164AsnCys: 0.164 ± 0.014	1.078AsnAsp: 1.078 ± 0.033	0.828AsnGlu: 0.828 ± 0.031	0.552AsnPhe: 0.552 ± 0.031	1.878AsnGly: 1.878 ± 0.054	0.373AsnHis: 0.373 ± 0.017	0.786AsnIle: 0.786 ± 0.033	0.38AsnLys: 0.38 ± 0.023	1.806AsnLeu: 1.806 ± 0.046	0.358AsnMet: 0.358 ± 0.022	0.498AsnAsn: 0.498 ± 0.029	1.337AsnPro: 1.337 ± 0.036	0.582AsnGln: 0.582 ± 0.029	1.292AsnArg: 1.292 ± 0.038	0.875AsnSer: 0.875 ± 0.03	1.06AsnThr: 1.06 ± 0.042	1.585AsnVal: 1.585 ± 0.052	0.329AsnTrp: 0.329 ± 0.021	0.437AsnTyr: 0.437 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	7.627ProAla: 7.627 ± 0.115	0.33ProCys: 0.33 ± 0.02	4.301ProAsp: 4.301 ± 0.063	3.775ProGlu: 3.775 ± 0.056	1.553ProPhe: 1.553 ± 0.041	5.748ProGly: 5.748 ± 0.087	1.302ProHis: 1.302 ± 0.045	1.958ProIle: 1.958 ± 0.043	1.036ProLys: 1.036 ± 0.033	5.001ProLeu: 5.001 ± 0.075	1.113ProMet: 1.113 ± 0.033	0.94ProAsn: 0.94 ± 0.034	2.766ProPro: 2.766 ± 0.066	1.839ProGln: 1.839 ± 0.043	3.939ProArg: 3.939 ± 0.081	3.419ProSer: 3.419 ± 0.076	3.811ProThr: 3.811 ± 0.065	5.209ProVal: 5.209 ± 0.07	0.964ProTrp: 0.964 ± 0.035	1.28ProTyr: 1.28 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.862GlnAla: 3.862 ± 0.074	0.165GlnCys: 0.165 ± 0.014	1.463GlnAsp: 1.463 ± 0.039	1.518GlnGlu: 1.518 ± 0.04	0.755GlnPhe: 0.755 ± 0.034	2.515GlnGly: 2.515 ± 0.052	0.681GlnHis: 0.681 ± 0.031	1.348GlnIle: 1.348 ± 0.036	0.59GlnLys: 0.59 ± 0.025	3.161GlnLeu: 3.161 ± 0.062	0.623GlnMet: 0.623 ± 0.025	0.579GlnAsn: 0.579 ± 0.027	1.648GlnPro: 1.648 ± 0.044	1.337GlnGln: 1.337 ± 0.039	2.604GlnArg: 2.604 ± 0.058	1.304GlnSer: 1.304 ± 0.039	1.491GlnThr: 1.491 ± 0.043	2.909GlnVal: 2.909 ± 0.046	0.482GlnTrp: 0.482 ± 0.024	0.64GlnTyr: 0.64 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	8.893ArgAla: 8.893 ± 0.136	0.563ArgCys: 0.563 ± 0.027	4.352ArgAsp: 4.352 ± 0.075	4.122ArgGlu: 4.122 ± 0.076	2.274ArgPhe: 2.274 ± 0.048	5.643ArgGly: 5.643 ± 0.098	1.947ArgHis: 1.947 ± 0.047	3.467ArgIle: 3.467 ± 0.061	1.388ArgLys: 1.388 ± 0.04	8.038ArgLeu: 8.038 ± 0.115	1.894ArgMet: 1.894 ± 0.046	1.342ArgAsn: 1.342 ± 0.037	4.687ArgPro: 4.687 ± 0.075	2.406ArgGln: 2.406 ± 0.057	7.785ArgArg: 7.785 ± 0.126	4.156ArgSer: 4.156 ± 0.078	5.281ArgThr: 5.281 ± 0.081	6.024ArgVal: 6.024 ± 0.077	1.416ArgTrp: 1.416 ± 0.043	1.601ArgTyr: 1.601 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.48SerAla: 6.48 ± 0.086	0.43SerCys: 0.43 ± 0.02	2.758SerAsp: 2.758 ± 0.047	2.263SerGlu: 2.263 ± 0.047	1.574SerPhe: 1.574 ± 0.043	5.854SerGly: 5.854 ± 0.09	1.143SerHis: 1.143 ± 0.029	2.164SerIle: 2.164 ± 0.052	0.954SerLys: 0.954 ± 0.041	4.994SerLeu: 4.994 ± 0.072	1.318SerMet: 1.318 ± 0.04	0.967SerAsn: 0.967 ± 0.035	3.405SerPro: 3.405 ± 0.078	1.375SerGln: 1.375 ± 0.04	3.996SerArg: 3.996 ± 0.064	3.333SerSer: 3.333 ± 0.072	3.589SerThr: 3.589 ± 0.066	4.425SerVal: 4.425 ± 0.071	0.991SerTrp: 0.991 ± 0.032	1.253SerTyr: 1.253 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	7.589ThrAla: 7.589 ± 0.096	0.442ThrCys: 0.442 ± 0.022	3.574ThrAsp: 3.574 ± 0.066	2.802ThrGlu: 2.802 ± 0.053	1.802ThrPhe: 1.802 ± 0.045	6.176ThrGly: 6.176 ± 0.108	1.245ThrHis: 1.245 ± 0.037	2.658ThrIle: 2.658 ± 0.059	1.24ThrLys: 1.24 ± 0.043	6.101ThrLeu: 6.101 ± 0.092	1.162ThrMet: 1.162 ± 0.034	1.109ThrAsn: 1.109 ± 0.034	4.333ThrPro: 4.333 ± 0.083	1.63ThrGln: 1.63 ± 0.049	4.128ThrArg: 4.128 ± 0.074	3.832ThrSer: 3.832 ± 0.075	4.315ThrThr: 4.315 ± 0.086	5.892ThrVal: 5.892 ± 0.082	1.037ThrTrp: 1.037 ± 0.032	1.606ThrTyr: 1.606 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	11.582ValAla: 11.582 ± 0.129	0.713ValCys: 0.713 ± 0.028	5.582ValAsp: 5.582 ± 0.084	4.76ValGlu: 4.76 ± 0.084	2.448ValPhe: 2.448 ± 0.059	7.672ValGly: 7.672 ± 0.112	1.914ValHis: 1.914 ± 0.043	3.85ValIle: 3.85 ± 0.071	1.557ValLys: 1.557 ± 0.047	9.736ValLeu: 9.736 ± 0.119	1.901ValMet: 1.901 ± 0.051	1.788ValAsn: 1.788 ± 0.046	5.115ValPro: 5.115 ± 0.086	2.085ValGln: 2.085 ± 0.046	6.756ValArg: 6.756 ± 0.101	4.734ValSer: 4.734 ± 0.08	5.886ValThr: 5.886 ± 0.098	9.353ValVal: 9.353 ± 0.141	1.098ValTrp: 1.098 ± 0.037	1.413ValTyr: 1.413 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.741TrpAla: 1.741 ± 0.043	0.144TrpCys: 0.144 ± 0.012	0.776TrpAsp: 0.776 ± 0.027	0.666TrpGlu: 0.666 ± 0.025	0.55TrpPhe: 0.55 ± 0.029	1.178TrpGly: 1.178 ± 0.04	0.361TrpHis: 0.361 ± 0.021	0.71TrpIle: 0.71 ± 0.029	0.287TrpLys: 0.287 ± 0.018	1.817TrpLeu: 1.817 ± 0.048	0.352TrpMet: 0.352 ± 0.021	0.336TrpAsn: 0.336 ± 0.021	0.883TrpPro: 0.883 ± 0.034	0.574TrpGln: 0.574 ± 0.024	1.361TrpArg: 1.361 ± 0.044	1.055TrpSer: 1.055 ± 0.037	1.025TrpThr: 1.025 ± 0.037	1.238TrpVal: 1.238 ± 0.032	0.388TrpTrp: 0.388 ± 0.022	0.338TrpTyr: 0.338 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.6TyrAla: 2.6 ± 0.058	0.166TyrCys: 0.166 ± 0.014	1.467TyrAsp: 1.467 ± 0.045	1.106TyrGlu: 1.106 ± 0.034	0.664TyrPhe: 0.664 ± 0.026	2.161TyrGly: 2.161 ± 0.056	0.409TyrHis: 0.409 ± 0.019	0.54TyrIle: 0.54 ± 0.024	0.331TyrLys: 0.331 ± 0.022	2.328TyrLeu: 2.328 ± 0.049	0.298TyrMet: 0.298 ± 0.017	0.397TyrAsn: 0.397 ± 0.024	1.093TyrPro: 1.093 ± 0.039	0.71TyrGln: 0.71 ± 0.032	1.64TyrArg: 1.64 ± 0.05	1.016TyrSer: 1.016 ± 0.036	1.092TyrThr: 1.092 ± 0.036	1.948TyrVal: 1.948 ± 0.046	0.352TyrTrp: 0.352 ± 0.021	0.507TyrTyr: 0.507 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2972 proteins (955293 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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