Mycobacterium phage Sagefire

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VEG6|A0A649VEG6_9CAUD Lysin A OS=Mycobacterium phage Sagefire OX=2656602 GN=10 PE=4 SV=1
MM1 pKa = 7.93KK2 pKa = 9.35YY3 pKa = 7.78TLRR6 pKa = 11.84VFGIPVLSFEE16 pKa = 4.47SAGTGAEE23 pKa = 3.56EE24 pKa = 5.43GYY26 pKa = 10.65INLTGGSFEE35 pKa = 4.65LAPEE39 pKa = 3.98EE40 pKa = 4.15PEE42 pKa = 3.9YY43 pKa = 11.13DD44 pKa = 3.29EE45 pKa = 5.61EE46 pKa = 4.95YY47 pKa = 10.82YY48 pKa = 10.93EE49 pKa = 4.22EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VG56|A0A649VG56_9CAUD Terminase large subunit OS=Mycobacterium phage Sagefire OX=2656602 GN=12 PE=4 SV=1
MM1 pKa = 7.25SWEE4 pKa = 3.97SSDD7 pKa = 4.4RR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 4.24RR11 pKa = 11.84LPADD15 pKa = 3.09WTRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.78VLRR25 pKa = 11.84AAGHH29 pKa = 6.46RR30 pKa = 11.84CQIRR34 pKa = 11.84YY35 pKa = 9.29PDD37 pKa = 3.72ICTGMATEE45 pKa = 4.27VDD47 pKa = 3.34HH48 pKa = 7.08VRR50 pKa = 11.84YY51 pKa = 9.51RR52 pKa = 11.84DD53 pKa = 3.7EE54 pKa = 4.41EE55 pKa = 4.64SPTQASCRR63 pKa = 11.84PCHH66 pKa = 5.76ARR68 pKa = 11.84KK69 pKa = 9.57SAMEE73 pKa = 4.09GVAQRR78 pKa = 11.84AKK80 pKa = 10.29LRR82 pKa = 11.84AMKK85 pKa = 9.94KK86 pKa = 9.63RR87 pKa = 11.84PPPRR91 pKa = 11.84HH92 pKa = 5.87PGRR95 pKa = 11.84RR96 pKa = 11.84SNN98 pKa = 3.52

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

16128

28

823

181.2

19.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.144 ± 0.432

0.67 ± 0.102

6.56 ± 0.236

6.535 ± 0.288

3.063 ± 0.152

8.544 ± 0.446

1.947 ± 0.193

4.886 ± 0.213

4.043 ± 0.24

8.687 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.125

3.15 ± 0.161

5.624 ± 0.247

3.212 ± 0.169

6.362 ± 0.425

5.921 ± 0.294

6.504 ± 0.272

7.211 ± 0.218

2.108 ± 0.144

2.772 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski