Amino acid dipepetide frequency for Escherichia phage HK75

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.748AlaAla: 9.748 ± 1.675
0.975AlaCys: 0.975 ± 0.385
6.115AlaAsp: 6.115 ± 0.706
6.026AlaGlu: 6.026 ± 0.854
2.747AlaPhe: 2.747 ± 0.52
7.621AlaGly: 7.621 ± 0.871
1.507AlaHis: 1.507 ± 0.454
7.356AlaIle: 7.356 ± 0.777
4.165AlaLys: 4.165 ± 0.567
6.647AlaLeu: 6.647 ± 0.856
3.633AlaMet: 3.633 ± 0.686
4.786AlaAsn: 4.786 ± 0.775
1.507AlaPro: 1.507 ± 0.31
4.874AlaGln: 4.874 ± 0.614
4.165AlaArg: 4.165 ± 0.672
6.735AlaSer: 6.735 ± 1.291
5.849AlaThr: 5.849 ± 0.839
5.672AlaVal: 5.672 ± 0.79
1.861AlaTrp: 1.861 ± 0.38
2.924AlaTyr: 2.924 ± 0.45
0.0AlaXaa: 0.0 ± 0.0
Cys
1.063CysAla: 1.063 ± 0.421
0.177CysCys: 0.177 ± 0.137
0.975CysAsp: 0.975 ± 0.29
0.709CysGlu: 0.709 ± 0.237
0.266CysPhe: 0.266 ± 0.169
1.152CysGly: 1.152 ± 0.36
0.266CysHis: 0.266 ± 0.164
0.709CysIle: 0.709 ± 0.244
0.709CysLys: 0.709 ± 0.28
0.709CysLeu: 0.709 ± 0.293
0.089CysMet: 0.089 ± 0.097
0.709CysAsn: 0.709 ± 0.308
0.443CysPro: 0.443 ± 0.23
0.443CysGln: 0.443 ± 0.192
0.443CysArg: 0.443 ± 0.245
0.62CysSer: 0.62 ± 0.229
0.443CysThr: 0.443 ± 0.24
0.62CysVal: 0.62 ± 0.256
0.266CysTrp: 0.266 ± 0.211
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.229AspAla: 5.229 ± 0.748
0.62AspCys: 0.62 ± 0.267
4.077AspAsp: 4.077 ± 0.615
3.013AspGlu: 3.013 ± 0.459
1.95AspPhe: 1.95 ± 0.449
5.229AspGly: 5.229 ± 0.741
0.443AspHis: 0.443 ± 0.208
2.924AspIle: 2.924 ± 0.459
3.722AspLys: 3.722 ± 0.545
5.051AspLeu: 5.051 ± 0.641
1.241AspMet: 1.241 ± 0.347
3.456AspAsn: 3.456 ± 0.481
1.684AspPro: 1.684 ± 0.353
2.304AspGln: 2.304 ± 0.467
3.811AspArg: 3.811 ± 0.612
3.899AspSer: 3.899 ± 0.644
2.127AspThr: 2.127 ± 0.477
4.52AspVal: 4.52 ± 0.929
0.886AspTrp: 0.886 ± 0.257
2.216AspTyr: 2.216 ± 0.394
0.0AspXaa: 0.0 ± 0.0
Glu
5.229GluAla: 5.229 ± 0.798
0.532GluCys: 0.532 ± 0.238
2.216GluAsp: 2.216 ± 0.529
3.988GluGlu: 3.988 ± 0.788
1.861GluPhe: 1.861 ± 0.344
4.165GluGly: 4.165 ± 0.625
0.798GluHis: 0.798 ± 0.227
3.545GluIle: 3.545 ± 0.458
4.342GluLys: 4.342 ± 0.598
5.406GluLeu: 5.406 ± 0.66
1.507GluMet: 1.507 ± 0.412
3.456GluAsn: 3.456 ± 0.422
2.127GluPro: 2.127 ± 0.489
3.899GluGln: 3.899 ± 0.64
2.836GluArg: 2.836 ± 0.511
3.988GluSer: 3.988 ± 0.605
3.722GluThr: 3.722 ± 0.581
3.368GluVal: 3.368 ± 0.62
0.709GluTrp: 0.709 ± 0.278
1.95GluTyr: 1.95 ± 0.485
0.0GluXaa: 0.0 ± 0.0
Phe
3.102PheAla: 3.102 ± 0.594
0.354PheCys: 0.354 ± 0.175
2.127PheAsp: 2.127 ± 0.465
1.595PheGlu: 1.595 ± 0.291
0.62PhePhe: 0.62 ± 0.262
2.393PheGly: 2.393 ± 0.373
0.443PheHis: 0.443 ± 0.192
2.127PheIle: 2.127 ± 0.527
1.595PheLys: 1.595 ± 0.431
1.507PheLeu: 1.507 ± 0.469
0.709PheMet: 0.709 ± 0.19
2.038PheAsn: 2.038 ± 0.365
1.329PhePro: 1.329 ± 0.349
0.709PheGln: 0.709 ± 0.236
1.772PheArg: 1.772 ± 0.421
2.747PheSer: 2.747 ± 0.475
2.924PheThr: 2.924 ± 0.503
1.861PheVal: 1.861 ± 0.391
0.532PheTrp: 0.532 ± 0.175
0.975PheTyr: 0.975 ± 0.312
0.0PheXaa: 0.0 ± 0.0
Gly
6.203GlyAla: 6.203 ± 0.974
0.798GlyCys: 0.798 ± 0.221
4.342GlyAsp: 4.342 ± 0.668
4.608GlyGlu: 4.608 ± 0.559
3.102GlyPhe: 3.102 ± 0.624
5.938GlyGly: 5.938 ± 0.723
1.152GlyHis: 1.152 ± 0.265
4.431GlyIle: 4.431 ± 0.595
4.254GlyLys: 4.254 ± 0.535
6.115GlyLeu: 6.115 ± 0.821
2.924GlyMet: 2.924 ± 0.417
4.342GlyAsn: 4.342 ± 0.769
1.507GlyPro: 1.507 ± 0.363
3.633GlyGln: 3.633 ± 0.54
4.431GlyArg: 4.431 ± 0.636
3.456GlySer: 3.456 ± 0.541
5.76GlyThr: 5.76 ± 0.789
4.786GlyVal: 4.786 ± 0.624
1.772GlyTrp: 1.772 ± 0.386
2.127GlyTyr: 2.127 ± 0.418
0.0GlyXaa: 0.0 ± 0.0
His
1.418HisAla: 1.418 ± 0.446
0.266HisCys: 0.266 ± 0.16
0.975HisAsp: 0.975 ± 0.291
0.709HisGlu: 0.709 ± 0.278
0.443HisPhe: 0.443 ± 0.231
1.241HisGly: 1.241 ± 0.358
0.266HisHis: 0.266 ± 0.154
0.62HisIle: 0.62 ± 0.278
0.798HisLys: 0.798 ± 0.217
1.418HisLeu: 1.418 ± 0.449
0.0HisMet: 0.0 ± 0.0
0.62HisAsn: 0.62 ± 0.236
0.709HisPro: 0.709 ± 0.275
0.709HisGln: 0.709 ± 0.247
1.507HisArg: 1.507 ± 0.331
0.709HisSer: 0.709 ± 0.271
0.62HisThr: 0.62 ± 0.251
1.063HisVal: 1.063 ± 0.431
0.177HisTrp: 0.177 ± 0.131
0.443HisTyr: 0.443 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
4.874IleAla: 4.874 ± 0.647
0.709IleCys: 0.709 ± 0.345
5.051IleAsp: 5.051 ± 0.582
3.456IleGlu: 3.456 ± 0.488
2.216IlePhe: 2.216 ± 0.55
3.545IleGly: 3.545 ± 0.637
0.975IleHis: 0.975 ± 0.304
2.836IleIle: 2.836 ± 0.565
2.393IleLys: 2.393 ± 0.382
4.608IleLeu: 4.608 ± 0.662
1.063IleMet: 1.063 ± 0.379
3.368IleAsn: 3.368 ± 0.502
2.216IlePro: 2.216 ± 0.483
2.57IleGln: 2.57 ± 0.577
3.899IleArg: 3.899 ± 0.671
5.229IleSer: 5.229 ± 0.755
3.899IleThr: 3.899 ± 0.429
3.102IleVal: 3.102 ± 0.651
0.62IleTrp: 0.62 ± 0.201
1.418IleTyr: 1.418 ± 0.32
0.0IleXaa: 0.0 ± 0.0
Lys
5.583LysAla: 5.583 ± 0.69
0.443LysCys: 0.443 ± 0.239
3.102LysAsp: 3.102 ± 0.667
3.633LysGlu: 3.633 ± 0.697
1.418LysPhe: 1.418 ± 0.423
3.545LysGly: 3.545 ± 0.573
0.532LysHis: 0.532 ± 0.264
2.57LysIle: 2.57 ± 0.435
3.545LysLys: 3.545 ± 0.742
3.633LysLeu: 3.633 ± 0.523
2.216LysMet: 2.216 ± 0.604
2.216LysAsn: 2.216 ± 0.355
2.747LysPro: 2.747 ± 0.574
3.279LysGln: 3.279 ± 0.703
3.811LysArg: 3.811 ± 0.748
4.077LysSer: 4.077 ± 0.788
3.102LysThr: 3.102 ± 0.43
3.988LysVal: 3.988 ± 0.554
1.152LysTrp: 1.152 ± 0.328
2.038LysTyr: 2.038 ± 0.439
0.0LysXaa: 0.0 ± 0.0
Leu
7.887LeuAla: 7.887 ± 0.941
1.152LeuCys: 1.152 ± 0.321
4.786LeuAsp: 4.786 ± 0.482
5.14LeuGlu: 5.14 ± 0.751
2.038LeuPhe: 2.038 ± 0.393
3.899LeuGly: 3.899 ± 0.749
0.975LeuHis: 0.975 ± 0.399
4.963LeuIle: 4.963 ± 0.593
4.786LeuLys: 4.786 ± 0.617
5.583LeuLeu: 5.583 ± 0.814
1.684LeuMet: 1.684 ± 0.41
3.899LeuAsn: 3.899 ± 0.739
3.899LeuPro: 3.899 ± 0.56
2.924LeuGln: 2.924 ± 0.535
5.938LeuArg: 5.938 ± 0.867
6.115LeuSer: 6.115 ± 0.677
5.051LeuThr: 5.051 ± 0.667
3.988LeuVal: 3.988 ± 0.599
0.532LeuTrp: 0.532 ± 0.242
2.216LeuTyr: 2.216 ± 0.371
0.0LeuXaa: 0.0 ± 0.0
Met
3.633MetAla: 3.633 ± 0.563
0.532MetCys: 0.532 ± 0.3
1.507MetAsp: 1.507 ± 0.308
1.684MetGlu: 1.684 ± 0.422
0.709MetPhe: 0.709 ± 0.259
1.772MetGly: 1.772 ± 0.387
0.443MetHis: 0.443 ± 0.188
1.063MetIle: 1.063 ± 0.306
2.038MetLys: 2.038 ± 0.442
2.393MetLeu: 2.393 ± 0.46
0.354MetMet: 0.354 ± 0.179
0.709MetAsn: 0.709 ± 0.267
1.241MetPro: 1.241 ± 0.25
1.684MetGln: 1.684 ± 0.36
1.684MetArg: 1.684 ± 0.346
2.304MetSer: 2.304 ± 0.526
1.772MetThr: 1.772 ± 0.473
1.063MetVal: 1.063 ± 0.323
0.443MetTrp: 0.443 ± 0.223
0.709MetTyr: 0.709 ± 0.238
0.0MetXaa: 0.0 ± 0.0
Asn
5.229AsnAla: 5.229 ± 0.641
0.532AsnCys: 0.532 ± 0.263
2.304AsnAsp: 2.304 ± 0.346
3.013AsnGlu: 3.013 ± 0.441
1.418AsnPhe: 1.418 ± 0.339
5.317AsnGly: 5.317 ± 0.902
1.063AsnHis: 1.063 ± 0.355
3.102AsnIle: 3.102 ± 0.51
2.659AsnLys: 2.659 ± 0.457
3.102AsnLeu: 3.102 ± 0.524
0.798AsnMet: 0.798 ± 0.246
2.393AsnAsn: 2.393 ± 0.494
2.481AsnPro: 2.481 ± 0.56
2.127AsnGln: 2.127 ± 0.375
1.861AsnArg: 1.861 ± 0.471
2.216AsnSer: 2.216 ± 0.602
3.545AsnThr: 3.545 ± 0.594
1.684AsnVal: 1.684 ± 0.511
0.975AsnTrp: 0.975 ± 0.243
1.418AsnTyr: 1.418 ± 0.434
0.0AsnXaa: 0.0 ± 0.0
Pro
3.545ProAla: 3.545 ± 0.51
0.266ProCys: 0.266 ± 0.155
1.595ProAsp: 1.595 ± 0.415
2.304ProGlu: 2.304 ± 0.412
1.418ProPhe: 1.418 ± 0.389
3.279ProGly: 3.279 ± 0.457
0.62ProHis: 0.62 ± 0.268
1.152ProIle: 1.152 ± 0.355
1.95ProLys: 1.95 ± 0.381
2.481ProLeu: 2.481 ± 0.759
1.418ProMet: 1.418 ± 0.468
1.152ProAsn: 1.152 ± 0.314
2.038ProPro: 2.038 ± 0.429
1.95ProGln: 1.95 ± 0.429
2.127ProArg: 2.127 ± 0.397
3.279ProSer: 3.279 ± 0.598
1.595ProThr: 1.595 ± 0.481
3.368ProVal: 3.368 ± 0.548
0.354ProTrp: 0.354 ± 0.224
1.063ProTyr: 1.063 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
5.229GlnAla: 5.229 ± 0.995
0.177GlnCys: 0.177 ± 0.124
1.684GlnAsp: 1.684 ± 0.408
2.57GlnGlu: 2.57 ± 0.349
1.152GlnPhe: 1.152 ± 0.317
2.57GlnGly: 2.57 ± 0.56
0.532GlnHis: 0.532 ± 0.218
2.924GlnIle: 2.924 ± 0.359
3.456GlnLys: 3.456 ± 0.688
4.254GlnLeu: 4.254 ± 0.71
1.595GlnMet: 1.595 ± 0.376
2.127GlnAsn: 2.127 ± 0.527
1.241GlnPro: 1.241 ± 0.343
3.19GlnGln: 3.19 ± 0.623
3.633GlnArg: 3.633 ± 0.618
3.722GlnSer: 3.722 ± 0.666
3.19GlnThr: 3.19 ± 0.782
3.456GlnVal: 3.456 ± 0.468
0.975GlnTrp: 0.975 ± 0.331
1.152GlnTyr: 1.152 ± 0.403
0.0GlnXaa: 0.0 ± 0.0
Arg
4.608ArgAla: 4.608 ± 0.9
0.532ArgCys: 0.532 ± 0.193
3.899ArgAsp: 3.899 ± 0.652
3.899ArgGlu: 3.899 ± 0.676
2.038ArgPhe: 2.038 ± 0.368
3.899ArgGly: 3.899 ± 0.624
1.684ArgHis: 1.684 ± 0.367
3.19ArgIle: 3.19 ± 0.583
4.874ArgLys: 4.874 ± 0.555
5.849ArgLeu: 5.849 ± 0.644
1.95ArgMet: 1.95 ± 0.488
2.924ArgAsn: 2.924 ± 0.455
1.418ArgPro: 1.418 ± 0.33
3.19ArgGln: 3.19 ± 0.64
3.102ArgArg: 3.102 ± 0.804
2.659ArgSer: 2.659 ± 0.439
3.633ArgThr: 3.633 ± 0.48
3.899ArgVal: 3.899 ± 0.63
1.595ArgTrp: 1.595 ± 0.403
2.304ArgTyr: 2.304 ± 0.425
0.0ArgXaa: 0.0 ± 0.0
Ser
6.292SerAla: 6.292 ± 1.155
0.443SerCys: 0.443 ± 0.22
3.988SerAsp: 3.988 ± 0.515
4.254SerGlu: 4.254 ± 0.593
2.481SerPhe: 2.481 ± 0.413
6.203SerGly: 6.203 ± 0.782
0.532SerHis: 0.532 ± 0.227
3.545SerIle: 3.545 ± 0.491
2.836SerLys: 2.836 ± 0.692
6.026SerLeu: 6.026 ± 0.629
1.861SerMet: 1.861 ± 0.428
2.481SerAsn: 2.481 ± 0.437
3.19SerPro: 3.19 ± 0.55
3.722SerGln: 3.722 ± 0.879
4.963SerArg: 4.963 ± 0.656
5.14SerSer: 5.14 ± 1.159
3.545SerThr: 3.545 ± 0.54
5.938SerVal: 5.938 ± 0.961
1.507SerTrp: 1.507 ± 0.323
1.329SerTyr: 1.329 ± 0.396
0.0SerXaa: 0.0 ± 0.0
Thr
6.647ThrAla: 6.647 ± 0.829
0.798ThrCys: 0.798 ± 0.31
3.722ThrAsp: 3.722 ± 0.49
3.19ThrGlu: 3.19 ± 0.457
2.924ThrPhe: 2.924 ± 0.574
5.76ThrGly: 5.76 ± 0.783
0.443ThrHis: 0.443 ± 0.181
3.988ThrIle: 3.988 ± 0.641
2.836ThrLys: 2.836 ± 0.545
3.279ThrLeu: 3.279 ± 0.486
1.507ThrMet: 1.507 ± 0.283
2.127ThrAsn: 2.127 ± 0.477
3.102ThrPro: 3.102 ± 0.708
2.659ThrGln: 2.659 ± 0.615
3.368ThrArg: 3.368 ± 0.547
4.254ThrSer: 4.254 ± 0.554
2.747ThrThr: 2.747 ± 0.546
4.165ThrVal: 4.165 ± 0.518
1.241ThrTrp: 1.241 ± 0.374
1.241ThrTyr: 1.241 ± 0.363
0.0ThrXaa: 0.0 ± 0.0
Val
5.14ValAla: 5.14 ± 0.716
0.62ValCys: 0.62 ± 0.291
3.19ValAsp: 3.19 ± 0.424
3.545ValGlu: 3.545 ± 0.464
1.684ValPhe: 1.684 ± 0.293
4.52ValGly: 4.52 ± 0.752
0.975ValHis: 0.975 ± 0.287
4.077ValIle: 4.077 ± 0.654
3.899ValLys: 3.899 ± 0.454
5.495ValLeu: 5.495 ± 0.77
2.038ValMet: 2.038 ± 0.343
3.279ValAsn: 3.279 ± 0.699
2.57ValPro: 2.57 ± 0.413
2.481ValGln: 2.481 ± 0.37
3.633ValArg: 3.633 ± 0.494
5.849ValSer: 5.849 ± 0.781
3.545ValThr: 3.545 ± 0.536
4.786ValVal: 4.786 ± 0.738
1.063ValTrp: 1.063 ± 0.336
1.595ValTyr: 1.595 ± 0.311
0.0ValXaa: 0.0 ± 0.0
Trp
0.975TrpAla: 0.975 ± 0.304
0.532TrpCys: 0.532 ± 0.242
0.975TrpAsp: 0.975 ± 0.319
0.975TrpGlu: 0.975 ± 0.286
0.532TrpPhe: 0.532 ± 0.228
1.241TrpGly: 1.241 ± 0.313
0.798TrpHis: 0.798 ± 0.201
0.709TrpIle: 0.709 ± 0.215
0.798TrpLys: 0.798 ± 0.22
2.038TrpLeu: 2.038 ± 0.5
0.62TrpMet: 0.62 ± 0.241
0.266TrpAsn: 0.266 ± 0.149
0.354TrpPro: 0.354 ± 0.168
0.62TrpGln: 0.62 ± 0.179
1.684TrpArg: 1.684 ± 0.345
1.241TrpSer: 1.241 ± 0.345
0.975TrpThr: 0.975 ± 0.401
1.241TrpVal: 1.241 ± 0.298
0.354TrpTrp: 0.354 ± 0.161
0.62TrpTyr: 0.62 ± 0.199
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.368TyrAla: 3.368 ± 0.508
0.354TyrCys: 0.354 ± 0.17
1.772TyrAsp: 1.772 ± 0.258
1.329TyrGlu: 1.329 ± 0.313
0.443TyrPhe: 0.443 ± 0.225
2.393TyrGly: 2.393 ± 0.439
0.266TyrHis: 0.266 ± 0.159
2.216TyrIle: 2.216 ± 0.432
0.975TyrLys: 0.975 ± 0.3
1.861TyrLeu: 1.861 ± 0.373
0.443TyrMet: 0.443 ± 0.213
0.886TyrAsn: 0.886 ± 0.255
1.152TyrPro: 1.152 ± 0.391
1.861TyrGln: 1.861 ± 0.348
2.481TyrArg: 2.481 ± 0.446
2.038TyrSer: 2.038 ± 0.539
1.95TyrThr: 1.95 ± 0.522
1.418TyrVal: 1.418 ± 0.365
0.532TyrTrp: 0.532 ± 0.207
0.798TyrTyr: 0.798 ± 0.243
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (11285 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski