Streptococcus phage phi5218

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1Q1A9|M1Q1A9_9CAUD Uncharacterized protein OS=Streptococcus phage phi5218 OX=1289597 GN=phi5218_0055 PE=4 SV=1
MM1 pKa = 7.63FDD3 pKa = 3.54YY4 pKa = 8.75DD5 pKa = 3.72TWLSTPPEE13 pKa = 4.38PPVDD17 pKa = 2.65IWKK20 pKa = 9.79IDD22 pKa = 3.61EE23 pKa = 4.82DD24 pKa = 3.7EE25 pKa = 4.38DD26 pKa = 4.15RR27 pKa = 11.84AYY29 pKa = 10.64DD30 pKa = 3.46EE31 pKa = 4.65WKK33 pKa = 10.76DD34 pKa = 3.89NVAMGYY40 pKa = 8.29EE41 pKa = 4.0

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1PRY8|M1PRY8_9CAUD Uncharacterized protein OS=Streptococcus phage phi5218 OX=1289597 GN=phi5218_0039 PE=4 SV=1
MM1 pKa = 6.39TTADD5 pKa = 3.75KK6 pKa = 10.59IKK8 pKa = 10.94YY9 pKa = 9.05ILQKK13 pKa = 9.81TGWTRR18 pKa = 11.84GQFATEE24 pKa = 3.84MDD26 pKa = 3.9VTTLSVYY33 pKa = 10.06KK34 pKa = 9.79WLDD37 pKa = 3.06GRR39 pKa = 11.84PPRR42 pKa = 11.84QRR44 pKa = 11.84MLDD47 pKa = 3.9KK48 pKa = 10.28IDD50 pKa = 3.69EE51 pKa = 4.79LYY53 pKa = 10.91EE54 pKa = 3.85QVKK57 pKa = 9.92PYY59 pKa = 9.67EE60 pKa = 4.45PKK62 pKa = 10.46VLAPRR67 pKa = 11.84GKK69 pKa = 9.48IRR71 pKa = 11.84LVYY74 pKa = 9.7PYY76 pKa = 9.9YY77 pKa = 10.39SHH79 pKa = 6.81QRR81 pKa = 11.84QPWGKK86 pKa = 9.78

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

11467

33

1408

179.2

20.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.898 ± 0.407

0.654 ± 0.112

5.982 ± 0.247

7.77 ± 0.494

4.003 ± 0.178

6.261 ± 0.485

1.788 ± 0.195

6.628 ± 0.254

7.953 ± 0.569

8.913 ± 0.524

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.607 ± 0.201

5.32 ± 0.285

2.869 ± 0.277

4.395 ± 0.211

4.709 ± 0.3

5.869 ± 0.451

6.218 ± 0.515

6.209 ± 0.236

1.125 ± 0.134

3.828 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski