Bordetella ansorpii
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5495 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A157SI34|A0A157SI34_9BORD Uncharacterized protein OS=Bordetella ansorpii OX=288768 GN=SAMEA3906486_02913 PE=4 SV=1
MM1 pKa = 7.52 LSAIEE6 pKa = 4.4 QLIQRR11 pKa = 11.84 NLADD15 pKa = 4.01 GSDD18 pKa = 3.38 IAVSAGVSQDD28 pKa = 3.72 MIQQAEE34 pKa = 4.27 SMLGVRR40 pKa = 11.84 FPVSYY45 pKa = 10.47 RR46 pKa = 11.84 AFLATYY52 pKa = 9.14 GAIEE56 pKa = 4.41 IDD58 pKa = 3.88 CQSFAGLTPEE68 pKa = 4.71 GKK70 pKa = 10.51 VGDD73 pKa = 4.34 DD74 pKa = 3.54 CDD76 pKa = 3.76 VVSFTRR82 pKa = 11.84 YY83 pKa = 9.59 ARR85 pKa = 11.84 EE86 pKa = 4.29 HH87 pKa = 6.57 YY88 pKa = 10.28 SLPDD92 pKa = 3.6 PYY94 pKa = 11.2 VALDD98 pKa = 3.8 FQDD101 pKa = 3.62 GDD103 pKa = 3.31 FWLCLDD109 pKa = 4.44 TSQVDD114 pKa = 3.81 AQGEE118 pKa = 4.73 SPVVLVSPVDD128 pKa = 3.75 GTRR131 pKa = 11.84 KK132 pKa = 9.85 GPAVAEE138 pKa = 4.31 SWASYY143 pKa = 7.9 LTSYY147 pKa = 11.21 LSAA150 pKa = 5.58
Molecular weight: 16.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.948
IPC_protein 3.923
Toseland 3.694
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.617
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.037
Patrickios 0.998
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.928
Protein with the highest isoelectric point:
>tr|A0A157M585|A0A157M585_9BORD 30S ribosomal protein S7 OS=Bordetella ansorpii OX=288768 GN=rpsG PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.39 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5495
0
5495
1836431
29
5643
334.2
36.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.119 ± 0.046
0.868 ± 0.011
5.584 ± 0.028
4.943 ± 0.029
3.21 ± 0.023
8.652 ± 0.056
2.168 ± 0.018
4.253 ± 0.024
2.642 ± 0.028
10.702 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.472 ± 0.02
2.509 ± 0.026
5.459 ± 0.031
4.062 ± 0.019
7.474 ± 0.042
5.404 ± 0.03
5.137 ± 0.047
7.563 ± 0.025
1.398 ± 0.015
2.381 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here