Macropodid alphaherpesvirus 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Y0AC57|A0A0Y0AC57_9ALPH Tegument protein OS=Macropodid alphaherpesvirus 1 OX=137443 GN=UL14 PE=3 SV=1
MM1 pKa = 7.65SPRR4 pKa = 11.84CSVRR8 pKa = 11.84PHH10 pKa = 6.48EE11 pKa = 4.51SVEE14 pKa = 3.98ALLEE18 pKa = 4.38PGAVQEE24 pKa = 4.3TSDD27 pKa = 4.05AEE29 pKa = 4.29EE30 pKa = 3.95VDD32 pKa = 3.44SDD34 pKa = 4.06EE35 pKa = 4.46EE36 pKa = 3.96QSEE39 pKa = 4.4GGEE42 pKa = 4.15SSEE45 pKa = 4.18EE46 pKa = 3.92DD47 pKa = 3.16SSEE50 pKa = 4.11EE51 pKa = 3.75NSEE54 pKa = 4.11LSDD57 pKa = 3.65EE58 pKa = 4.28EE59 pKa = 4.4FSDD62 pKa = 4.6GEE64 pKa = 4.16EE65 pKa = 4.89SSDD68 pKa = 3.3EE69 pKa = 3.87DD70 pKa = 3.74DD71 pKa = 3.43RR72 pKa = 11.84RR73 pKa = 11.84GRR75 pKa = 11.84TRR77 pKa = 11.84RR78 pKa = 11.84QSTKK82 pKa = 9.07TAVLEE87 pKa = 4.1FLDD90 pKa = 4.46LEE92 pKa = 4.71AIEE95 pKa = 4.61VGGGSPEE102 pKa = 4.12YY103 pKa = 10.83GCEE106 pKa = 3.52DD107 pKa = 3.94DD108 pKa = 5.41YY109 pKa = 11.68YY110 pKa = 11.55DD111 pKa = 3.86RR112 pKa = 11.84YY113 pKa = 10.89EE114 pKa = 4.12VFSEE118 pKa = 3.97EE119 pKa = 4.66DD120 pKa = 3.03SDD122 pKa = 4.07EE123 pKa = 4.46GVSEE127 pKa = 4.36EE128 pKa = 4.25DD129 pKa = 3.48HH130 pKa = 7.18KK131 pKa = 11.56DD132 pKa = 3.39ALGCGPSDD140 pKa = 4.15SKK142 pKa = 11.64LPDD145 pKa = 3.82LLSDD149 pKa = 4.13VPEE152 pKa = 4.5HH153 pKa = 6.68PANKK157 pKa = 10.19GEE159 pKa = 4.15SRR161 pKa = 11.84SNRR164 pKa = 11.84EE165 pKa = 3.19ISEE168 pKa = 4.38DD169 pKa = 3.45EE170 pKa = 4.6SICEE174 pKa = 3.76EE175 pKa = 4.08LVGFRR180 pKa = 11.84HH181 pKa = 6.4KK182 pKa = 10.27RR183 pKa = 11.84KK184 pKa = 8.52KK185 pKa = 7.56WHH187 pKa = 5.51VVEE190 pKa = 5.1SSSEE194 pKa = 3.93SDD196 pKa = 3.3EE197 pKa = 5.94DD198 pKa = 3.9SDD200 pKa = 4.88EE201 pKa = 4.65PEE203 pKa = 4.32STSKK207 pKa = 10.12PRR209 pKa = 11.84KK210 pKa = 8.03LLKK213 pKa = 10.05RR214 pKa = 11.84LRR216 pKa = 11.84RR217 pKa = 11.84QVDD220 pKa = 3.59GEE222 pKa = 4.5GNIASQRR229 pKa = 11.84SATSEE234 pKa = 3.46GRR236 pKa = 11.84QIVSGPDD243 pKa = 3.39NQPGEE248 pKa = 4.36PGASGRR254 pKa = 11.84RR255 pKa = 11.84PVKK258 pKa = 10.56RR259 pKa = 11.84NPTWSEE265 pKa = 3.47LQQNINQLFRR275 pKa = 11.84FLRR278 pKa = 11.84DD279 pKa = 3.26KK280 pKa = 11.05PNGRR284 pKa = 11.84GRR286 pKa = 11.84IDD288 pKa = 3.38RR289 pKa = 11.84LRR291 pKa = 11.84DD292 pKa = 3.57VVIDD296 pKa = 4.14IYY298 pKa = 11.62LMGYY302 pKa = 7.11EE303 pKa = 4.55RR304 pKa = 11.84RR305 pKa = 11.84RR306 pKa = 11.84LDD308 pKa = 3.22PTGWGVLLQTTGHH321 pKa = 5.98RR322 pKa = 11.84TDD324 pKa = 3.93AVRR327 pKa = 11.84DD328 pKa = 4.17DD329 pKa = 3.72IGGVEE334 pKa = 4.69CLVGVPGPTDD344 pKa = 3.26SLRR347 pKa = 11.84PLAGKK352 pKa = 10.32SYY354 pKa = 8.92GQCGSGGVPEE364 pKa = 4.62TSDD367 pKa = 3.16IDD369 pKa = 3.94SDD371 pKa = 3.71ASTVVVNTKK380 pKa = 10.04GRR382 pKa = 11.84WKK384 pKa = 9.66TSGNAARR391 pKa = 11.84LDD393 pKa = 3.54EE394 pKa = 4.33EE395 pKa = 4.67TRR397 pKa = 11.84VGSPLRR403 pKa = 11.84DD404 pKa = 3.27SPRR407 pKa = 11.84RR408 pKa = 11.84GEE410 pKa = 4.37LDD412 pKa = 3.43ASPSMATQPWPSMVVADD429 pKa = 4.72TDD431 pKa = 4.27STDD434 pKa = 3.03LWSQSTGQTFPVFKK448 pKa = 9.53DD449 pKa = 3.3TKK451 pKa = 9.37GCCEE455 pKa = 3.84IQFPAPRR462 pKa = 11.84RR463 pKa = 3.7

Molecular weight: 51.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A120HUJ2|A0A120HUJ2_9ALPH Isoform of A0A0Y0A7L1 GG OS=Macropodid alphaherpesvirus 1 OX=137443 GN=US4 PE=4 SV=1
MM1 pKa = 7.85PATSPSIPLPRR12 pKa = 11.84ATPIRR17 pKa = 11.84IRR19 pKa = 11.84TRR21 pKa = 11.84PRR23 pKa = 11.84RR24 pKa = 11.84PPIRR28 pKa = 11.84PTGLTNARR36 pKa = 11.84RR37 pKa = 11.84SPGPGTGDD45 pKa = 3.0LSLRR49 pKa = 11.84SRR51 pKa = 11.84VVRR54 pKa = 11.84RR55 pKa = 11.84KK56 pKa = 7.98RR57 pKa = 11.84TCVRR61 pKa = 11.84SAPTPFTDD69 pKa = 4.1AVRR72 pKa = 11.84ATTFPCLHH80 pKa = 6.3NFCVACLKK88 pKa = 9.67TWLPQHH94 pKa = 5.82NRR96 pKa = 11.84CPLCNLSVSYY106 pKa = 10.28LIVDD110 pKa = 3.69VRR112 pKa = 11.84PDD114 pKa = 3.44GTFSTIPVVRR124 pKa = 11.84QQDD127 pKa = 4.18LYY129 pKa = 11.11GEE131 pKa = 4.06PAAAVRR137 pKa = 11.84AGTAVDD143 pKa = 5.06FIWTGAARR151 pKa = 11.84QAPASVSAGGYY162 pKa = 4.93TVRR165 pKa = 11.84ARR167 pKa = 11.84SPRR170 pKa = 11.84RR171 pKa = 11.84SIGSSDD177 pKa = 3.25GANRR181 pKa = 11.84PTRR184 pKa = 11.84PVGRR188 pKa = 11.84PRR190 pKa = 11.84RR191 pKa = 11.84RR192 pKa = 11.84RR193 pKa = 11.84RR194 pKa = 11.84SNQVSPRR201 pKa = 11.84ADD203 pKa = 4.05PIVIDD208 pKa = 5.13DD209 pKa = 5.19DD210 pKa = 4.58EE211 pKa = 7.53DD212 pKa = 5.48DD213 pKa = 4.73GAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPRR259 pKa = 11.84PHH261 pKa = 6.5ATVTPQPRR269 pKa = 11.84TLWTSEE275 pKa = 3.77TGTSGLEE282 pKa = 3.99GPRR285 pKa = 11.84ATRR288 pKa = 11.84VRR290 pKa = 11.84VEE292 pKa = 3.99VLCNIARR299 pKa = 11.84TEE301 pKa = 4.1EE302 pKa = 4.12PQRR305 pKa = 11.84TPTASPDD312 pKa = 3.26ATPVHH317 pKa = 6.74SSTVPEE323 pKa = 4.1EE324 pKa = 4.36TPAGASSVPAPAPVRR339 pKa = 11.84KK340 pKa = 9.46RR341 pKa = 11.84RR342 pKa = 11.84LATEE346 pKa = 4.1NRR348 pKa = 11.84ASSPDD353 pKa = 3.37TRR355 pKa = 11.84GRR357 pKa = 11.84PAADD361 pKa = 3.56PRR363 pKa = 11.84PGTSGAAEE371 pKa = 4.06EE372 pKa = 4.97GEE374 pKa = 4.41EE375 pKa = 4.25EE376 pKa = 4.72DD377 pKa = 5.63GEE379 pKa = 4.93GPTPSDD385 pKa = 3.16PRR387 pKa = 11.84AGRR390 pKa = 11.84DD391 pKa = 3.43QPCRR395 pKa = 11.84KK396 pKa = 9.3CVRR399 pKa = 11.84KK400 pKa = 7.34THH402 pKa = 6.03HH403 pKa = 6.75RR404 pKa = 11.84SALAAEE410 pKa = 4.5RR411 pKa = 11.84PVEE414 pKa = 4.33GSFLQYY420 pKa = 10.45RR421 pKa = 11.84PLVGLSSAVAMAPFLNKK438 pKa = 9.33TVTGDD443 pKa = 3.52CLPIVDD449 pKa = 5.23LEE451 pKa = 4.3SGEE454 pKa = 3.81IGAYY458 pKa = 9.55VVLTHH463 pKa = 6.12QDD465 pKa = 3.2CNMARR470 pKa = 11.84ALAEE474 pKa = 4.36ARR476 pKa = 11.84STWLDD481 pKa = 3.61DD482 pKa = 3.48TSLPLTNPRR491 pKa = 11.84GATGSDD497 pKa = 3.57VPRR500 pKa = 11.84EE501 pKa = 4.11RR502 pKa = 11.84PGSPFRR508 pKa = 11.84LWSTSRR514 pKa = 11.84GTLLFRR520 pKa = 11.84RR521 pKa = 11.84DD522 pKa = 2.94GTLYY526 pKa = 10.65GRR528 pKa = 11.84QSPFGGVQLHH538 pKa = 6.55PWDD541 pKa = 3.83PP542 pKa = 3.55

Molecular weight: 57.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

1

71

37452

81

2844

527.5

58.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.243 ± 0.255

1.784 ± 0.12

5.193 ± 0.145

4.937 ± 0.156

3.917 ± 0.155

5.815 ± 0.177

2.766 ± 0.092

5.281 ± 0.165

3.196 ± 0.152

10.157 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.864 ± 0.107

3.482 ± 0.124

7.847 ± 0.327

3.901 ± 0.106

6.723 ± 0.199

7.148 ± 0.188

7.327 ± 0.211

6.227 ± 0.138

1.143 ± 0.061

3.049 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski