Pseudopuniceibacterium sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudopuniceibacterium

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3986 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A399J462|A0A399J462_9RHOB Nicotinate phosphoribosyltransferase OS=Pseudopuniceibacterium sediminis OX=2211117 GN=pncB PE=3 SV=1
MM1 pKa = 7.48ILCTTRR7 pKa = 11.84FAKK10 pKa = 9.86IRR12 pKa = 11.84PTARR16 pKa = 11.84VGLGVACALTLSLCGPAAMAEE37 pKa = 4.09QKK39 pKa = 9.69TDD41 pKa = 4.38FKK43 pKa = 11.23LAWTIYY49 pKa = 9.43AGWMPWGYY57 pKa = 10.22IEE59 pKa = 5.18TSGIMDD65 pKa = 3.89KK66 pKa = 10.61WADD69 pKa = 3.4KK70 pKa = 11.31YY71 pKa = 11.2GITVDD76 pKa = 3.47IVQLNDD82 pKa = 3.23YY83 pKa = 10.37VEE85 pKa = 5.22SINQYY90 pKa = 8.14TAGAFDD96 pKa = 4.9GVTATNMDD104 pKa = 4.33ALSIPAGGGVDD115 pKa = 3.6STALIVGDD123 pKa = 3.76YY124 pKa = 11.35SNGNDD129 pKa = 3.81AIIVKK134 pKa = 10.28GDD136 pKa = 3.14VGLADD141 pKa = 4.1LAGKK145 pKa = 9.51SVNLVEE151 pKa = 5.48LSVSHH156 pKa = 6.06YY157 pKa = 10.83LLARR161 pKa = 11.84ALEE164 pKa = 4.13QVDD167 pKa = 4.88LSEE170 pKa = 5.45ADD172 pKa = 3.45LGAVINTSDD181 pKa = 4.2ADD183 pKa = 3.85MIAAFQTDD191 pKa = 3.72DD192 pKa = 3.86VEE194 pKa = 6.07VVVTWNPLVSTILEE208 pKa = 4.47DD209 pKa = 3.78PGATKK214 pKa = 10.51VYY216 pKa = 10.06DD217 pKa = 3.56SSDD220 pKa = 3.3IPGEE224 pKa = 4.2IIDD227 pKa = 4.39CLFVNTDD234 pKa = 3.46TLADD238 pKa = 3.46NPAFGKK244 pKa = 10.51AVTGAWYY251 pKa = 8.26EE252 pKa = 4.33TMALMASGTPEE263 pKa = 3.72GDD265 pKa = 3.23AALSEE270 pKa = 4.29MAEE273 pKa = 3.99ASGTDD278 pKa = 3.36LEE280 pKa = 5.16GYY282 pKa = 9.91KK283 pKa = 10.31SQLAATAMFYY293 pKa = 10.12TPKK296 pKa = 10.63AAMEE300 pKa = 4.18FTEE303 pKa = 4.88GASLQTTMVSVANFLFDD320 pKa = 4.46KK321 pKa = 11.1GILGEE326 pKa = 4.43GAPSPDD332 pKa = 3.94YY333 pKa = 11.45VGVAYY338 pKa = 9.61PDD340 pKa = 3.78GATTGDD346 pKa = 3.47AGNVNLRR353 pKa = 11.84YY354 pKa = 8.38DD355 pKa = 3.6TTYY358 pKa = 11.13VAMAVDD364 pKa = 4.42GTLL367 pKa = 3.25

Molecular weight: 38.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A399IVK0|A0A399IVK0_9RHOB D-glutamate deacylase OS=Pseudopuniceibacterium sediminis OX=2211117 GN=DL237_19210 PE=4 SV=1
MM1 pKa = 7.64PVFLFGSARR10 pKa = 11.84FRR12 pKa = 11.84QVRR15 pKa = 11.84FRR17 pKa = 11.84QVRR20 pKa = 11.84FGSARR25 pKa = 11.84FGSARR30 pKa = 11.84FGSARR35 pKa = 11.84FGSARR40 pKa = 11.84FGSARR45 pKa = 11.84FGSARR50 pKa = 11.84FGSAKK55 pKa = 10.16FGSAKK60 pKa = 10.21FGSAKK65 pKa = 9.51FRR67 pKa = 11.84QVSRR71 pKa = 11.84AGSS74 pKa = 3.24

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3986

0

3986

1282358

28

2798

321.7

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.562 ± 0.055

0.875 ± 0.012

6.068 ± 0.033

5.49 ± 0.039

3.624 ± 0.029

8.741 ± 0.036

2.043 ± 0.021

5.178 ± 0.03

2.844 ± 0.03

10.227 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.881 ± 0.021

2.456 ± 0.024

5.199 ± 0.031

3.284 ± 0.026

6.879 ± 0.038

5.273 ± 0.029

5.585 ± 0.032

7.245 ± 0.027

1.349 ± 0.017

2.197 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski