Streptococcus satellite phage Javan267
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHN0|A0A4D5ZHN0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan267 OX=2558598 GN=JavanS267_0013 PE=4 SV=1
MM1 pKa = 7.27 NRR3 pKa = 11.84 LKK5 pKa = 10.56 EE6 pKa = 3.91 LRR8 pKa = 11.84 QEE10 pKa = 4.15 KK11 pKa = 10.33 KK12 pKa = 10.62 LSQKK16 pKa = 10.35 EE17 pKa = 3.78 IAEE20 pKa = 4.22 TLGFSLRR27 pKa = 11.84 SFQRR31 pKa = 11.84 MEE33 pKa = 3.91 NGEE36 pKa = 4.05 NQIKK40 pKa = 9.89 PEE42 pKa = 4.02 KK43 pKa = 9.71 AQQLADD49 pKa = 3.69 YY50 pKa = 9.64 FGVSVGYY57 pKa = 10.38 LLGYY61 pKa = 9.63 SDD63 pKa = 4.55 VRR65 pKa = 11.84 FEE67 pKa = 4.9 LEE69 pKa = 4.07 QIEE72 pKa = 4.39 KK73 pKa = 10.37 AIKK76 pKa = 9.29 NRR78 pKa = 11.84 LMDD81 pKa = 4.11 SEE83 pKa = 4.47 STINKK88 pKa = 9.68 DD89 pKa = 3.4 VFEE92 pKa = 4.39 NTLKK96 pKa = 10.47 IVEE99 pKa = 4.32 LARR102 pKa = 11.84 FLDD105 pKa = 4.02 LDD107 pKa = 4.55 LDD109 pKa = 4.81 TITSLYY115 pKa = 10.34 FYY117 pKa = 10.05 NCEE120 pKa = 3.38 ISKK123 pKa = 10.5 IKK125 pKa = 9.37 TLNDD129 pKa = 3.02 LFDD132 pKa = 4.8 FFDD135 pKa = 4.7 SEE137 pKa = 4.12 AHH139 pKa = 6.67 GYY141 pKa = 8.53 MEE143 pKa = 5.16 LLSIVEE149 pKa = 4.78 PYY151 pKa = 10.1 QADD154 pKa = 4.08 EE155 pKa = 4.71 IIQEE159 pKa = 3.99 ISKK162 pKa = 10.87 LGDD165 pKa = 3.44 YY166 pKa = 10.73 KK167 pKa = 11.3 EE168 pKa = 4.12 EE169 pKa = 4.04 LSLYY173 pKa = 9.09 LDD175 pKa = 3.43 KK176 pKa = 11.34 VRR178 pKa = 11.84 AEE180 pKa = 3.71 EE181 pKa = 4.9 DD182 pKa = 3.6 FFNVTGYY189 pKa = 9.94 PITIEE194 pKa = 3.77 TDD196 pKa = 3.67 EE197 pKa = 4.48 IPNNVVKK204 pKa = 10.66 INRR207 pKa = 11.84 IEE209 pKa = 3.92 NTLSDD214 pKa = 4.56 KK215 pKa = 11.1 EE216 pKa = 4.61 LMALPPQKK224 pKa = 10.26 RR225 pKa = 11.84 KK226 pKa = 10.0 EE227 pKa = 3.93 YY228 pKa = 10.4 LKK230 pKa = 11.19 NFFDD234 pKa = 4.25 EE235 pKa = 4.74 VNDD238 pKa = 4.15 ALSSLTDD245 pKa = 4.32 NIDD248 pKa = 3.54 DD249 pKa = 4.98 AIKK252 pKa = 9.57 TVSQNQVDD260 pKa = 4.23 LLINNLIKK268 pKa = 10.75 ALAKK272 pKa = 10.95 LNDD275 pKa = 4.08 VKK277 pKa = 11.44 NKK279 pKa = 10.22 DD280 pKa = 3.2
Molecular weight: 32.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.671
IPC2_protein 4.584
IPC_protein 4.52
Toseland 4.355
ProMoST 4.584
Dawson 4.457
Bjellqvist 4.622
Wikipedia 4.329
Rodwell 4.355
Grimsley 4.266
Solomon 4.457
Lehninger 4.418
Nozaki 4.571
DTASelect 4.724
Thurlkill 4.355
EMBOSS 4.342
Sillero 4.622
Patrickios 4.101
IPC_peptide 4.469
IPC2_peptide 4.622
IPC2.peptide.svr19 4.571
Protein with the highest isoelectric point:
>tr|A0A4D5ZHL8|A0A4D5ZHL8_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan267 OX=2558598 GN=JavanS267_0003 PE=4 SV=1
MM1 pKa = 7.43 EE2 pKa = 5.07 VIKK5 pKa = 10.82 SITIEE10 pKa = 4.16 TFIKK14 pKa = 10.24 PMKK17 pKa = 9.81 NKK19 pKa = 10.25 NISHH23 pKa = 7.33 GIAEE27 pKa = 4.31 LDD29 pKa = 3.6 GRR31 pKa = 11.84 KK32 pKa = 9.93 LEE34 pKa = 4.22 IDD36 pKa = 4.08 LDD38 pKa = 3.71 NLYY41 pKa = 8.93 ITFGSNHH48 pKa = 6.29 FDD50 pKa = 3.63 LARR53 pKa = 11.84 IPGTKK58 pKa = 9.74 GGNRR62 pKa = 11.84 YY63 pKa = 9.04 FFLCPICGNRR73 pKa = 11.84 CRR75 pKa = 11.84 KK76 pKa = 9.38 LYY78 pKa = 10.47 KK79 pKa = 10.21 RR80 pKa = 11.84 LFQYY84 pKa = 11.26 GCGSCQKK91 pKa = 9.71 IHH93 pKa = 7.2 KK94 pKa = 8.43 STLNRR99 pKa = 11.84 SKK101 pKa = 9.89 TDD103 pKa = 3.25 CQYY106 pKa = 10.79 YY107 pKa = 8.91 WEE109 pKa = 4.49 LALRR113 pKa = 11.84 EE114 pKa = 4.13 ARR116 pKa = 11.84 KK117 pKa = 9.93 VEE119 pKa = 4.41 PGWSPKK125 pKa = 9.98 RR126 pKa = 11.84 GGYY129 pKa = 8.86 MFDD132 pKa = 4.06 SFPEE136 pKa = 3.89 RR137 pKa = 11.84 PKK139 pKa = 9.48 YY140 pKa = 9.67 MKK142 pKa = 9.96 RR143 pKa = 11.84 KK144 pKa = 9.49 RR145 pKa = 11.84 YY146 pKa = 8.21 YY147 pKa = 9.09 KK148 pKa = 10.15 HH149 pKa = 4.77 YY150 pKa = 10.44 QKK152 pKa = 10.67 FVNYY156 pKa = 7.57 TRR158 pKa = 11.84 KK159 pKa = 10.24 GDD161 pKa = 4.06 SFWLNGLSSLRR172 pKa = 3.78
Molecular weight: 20.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.211
IPC2_protein 9.326
IPC_protein 9.297
Toseland 9.882
ProMoST 9.692
Dawson 10.131
Bjellqvist 9.838
Wikipedia 10.306
Rodwell 10.511
Grimsley 10.204
Solomon 10.145
Lehninger 10.116
Nozaki 9.955
DTASelect 9.809
Thurlkill 9.97
EMBOSS 10.306
Sillero 10.058
Patrickios 9.663
IPC_peptide 10.145
IPC2_peptide 8.668
IPC2.peptide.svr19 8.186
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2669
57
491
190.6
22.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.695 ± 0.464
0.787 ± 0.226
5.283 ± 0.46
8.28 ± 0.833
4.496 ± 0.336
4.833 ± 0.479
1.798 ± 0.296
7.119 ± 0.478
9.067 ± 0.372
10.229 ± 0.64
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.323 ± 0.348
5.283 ± 0.507
3.597 ± 0.543
3.672 ± 0.247
5.283 ± 0.489
5.807 ± 0.431
5.92 ± 0.34
5.133 ± 0.609
0.787 ± 0.186
4.608 ± 0.416
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here