Gordonia phage Chikenjars
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J6D934|A0A5J6D934_9CAUD Uncharacterized protein OS=Gordonia phage Chikenjars OX=2601686 GN=61 PE=4 SV=1
MM1 pKa = 7.08 TRR3 pKa = 11.84 IMWNSIGQRR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 9.83 EE15 pKa = 3.89 VGIDD19 pKa = 3.19 RR20 pKa = 11.84 GVLYY24 pKa = 10.62 LPQEE28 pKa = 4.91 GIAVPWNGLTSVSDD42 pKa = 3.63 VSDD45 pKa = 3.52 TVVEE49 pKa = 3.82 PLYY52 pKa = 11.11 FNGIKK57 pKa = 10.65 YY58 pKa = 10.0 YY59 pKa = 10.72 DD60 pKa = 3.63 YY61 pKa = 11.43 VSRR64 pKa = 11.84 GDD66 pKa = 3.52 YY67 pKa = 10.72 KK68 pKa = 10.83 GTLKK72 pKa = 10.92 AFTYY76 pKa = 10.28 PEE78 pKa = 4.17 EE79 pKa = 4.39 FEE81 pKa = 5.79 LYY83 pKa = 10.68 DD84 pKa = 3.86 GVQEE88 pKa = 4.19 SGNGIFVTGQIPTGVFHH105 pKa = 7.35 LSYY108 pKa = 10.01 RR109 pKa = 11.84 TMIGDD114 pKa = 5.03 DD115 pKa = 3.22 IDD117 pKa = 4.41 GVGGGYY123 pKa = 9.95 KK124 pKa = 9.47 IHH126 pKa = 6.39 VLYY129 pKa = 11.03 NLTAKK134 pKa = 10.21 PSNKK138 pKa = 9.39 TYY140 pKa = 9.94 STINNSQAAQEE151 pKa = 4.33 FSWEE155 pKa = 4.03 LSSVPMAGLNLRR167 pKa = 11.84 PSSHH171 pKa = 7.22 IIFDD175 pKa = 3.69 TTKK178 pKa = 7.99 MHH180 pKa = 7.25 EE181 pKa = 3.86 IAIAEE186 pKa = 4.29 VEE188 pKa = 4.13 QALYY192 pKa = 8.57 GTDD195 pKa = 3.43 TQDD198 pKa = 3.43 PQMKK202 pKa = 9.57 TIDD205 pKa = 3.71 EE206 pKa = 4.64 FEE208 pKa = 5.25 FITTTAAEE216 pKa = 4.15 IEE218 pKa = 4.34 IVDD221 pKa = 4.25 NGDD224 pKa = 3.83 GTWSASGSDD233 pKa = 3.79 YY234 pKa = 10.77 FIKK237 pKa = 10.53 QMPGVGLFTIKK248 pKa = 10.43 EE249 pKa = 3.8 ADD251 pKa = 3.22 AVYY254 pKa = 10.98 VDD256 pKa = 4.43 DD257 pKa = 4.06 EE258 pKa = 4.72 TYY260 pKa = 10.49 EE261 pKa = 3.94 ISTTEE266 pKa = 3.77 SS267 pKa = 2.95
Molecular weight: 29.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.069
IPC2_protein 4.266
IPC_protein 4.215
Toseland 4.037
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.062
Rodwell 4.05
Grimsley 3.948
Solomon 4.177
Lehninger 4.126
Nozaki 4.291
DTASelect 4.469
Thurlkill 4.062
EMBOSS 4.075
Sillero 4.329
Patrickios 2.626
IPC_peptide 4.177
IPC2_peptide 4.317
IPC2.peptide.svr19 4.243
Protein with the highest isoelectric point:
>tr|A0A5J6D936|A0A5J6D936_9CAUD Uncharacterized protein OS=Gordonia phage Chikenjars OX=2601686 GN=65 PE=4 SV=1
MM1 pKa = 7.42 ASKK4 pKa = 10.42 IITHH8 pKa = 7.24 IITGEE13 pKa = 3.57 KK14 pKa = 9.94 RR15 pKa = 11.84 KK16 pKa = 9.54 IVRR19 pKa = 11.84 TAIDD23 pKa = 3.1 PRR25 pKa = 11.84 YY26 pKa = 10.13 YY27 pKa = 10.71 VVSDD31 pKa = 3.45 LNGRR35 pKa = 11.84 GQRR38 pKa = 11.84 IMRR41 pKa = 11.84 KK42 pKa = 7.49 STVAIEE48 pKa = 4.09 WSTSVV53 pKa = 3.38
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.838
IPC_protein 10.643
Toseland 10.804
ProMoST 10.482
Dawson 10.891
Bjellqvist 10.584
Wikipedia 11.096
Rodwell 11.169
Grimsley 10.935
Solomon 11.008
Lehninger 10.979
Nozaki 10.76
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.199
Sillero 10.818
Patrickios 10.95
IPC_peptide 11.023
IPC2_peptide 9.355
IPC2.peptide.svr19 8.537
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
92
0
92
19206
30
1703
208.8
23.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.659 ± 0.533
0.609 ± 0.109
6.071 ± 0.284
6.92 ± 0.327
3.52 ± 0.225
7.581 ± 0.404
2.088 ± 0.213
5.852 ± 0.263
5.936 ± 0.342
8.096 ± 0.382
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.76 ± 0.124
4.394 ± 0.171
4.207 ± 0.293
3.457 ± 0.147
4.941 ± 0.348
6.644 ± 0.201
6.488 ± 0.242
7.196 ± 0.214
1.307 ± 0.119
3.275 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here