CRESS virus sp. ctf7a5
Average proteome isoelectric point is 7.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W902|A0A5Q2W902_9VIRU Putative capsid protein OS=CRESS virus sp. ctf7a5 OX=2656684 PE=4 SV=1
MM1 pKa = 7.93 PSTNYY6 pKa = 10.35 RR7 pKa = 11.84 YY8 pKa = 10.01 LSYY11 pKa = 10.69 MNYY14 pKa = 10.39 LGNKK18 pKa = 7.1 WLWIKK23 pKa = 10.89 HH24 pKa = 5.82 FGDD27 pKa = 3.32 TAAQLEE33 pKa = 4.4 YY34 pKa = 10.78 LDD36 pKa = 5.07 DD37 pKa = 5.33 HH38 pKa = 9.02 IEE40 pKa = 4.03 MEE42 pKa = 4.43 EE43 pKa = 4.26 TFPLTQVRR51 pKa = 11.84 SRR53 pKa = 11.84 GGNLINEE60 pKa = 4.67 LRR62 pKa = 11.84 PGSRR66 pKa = 11.84 QNPIVIEE73 pKa = 4.07
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.354
IPC2_protein 5.512
IPC_protein 5.359
Toseland 5.601
ProMoST 5.194
Dawson 5.474
Bjellqvist 5.588
Wikipedia 5.397
Rodwell 5.436
Grimsley 5.677
Solomon 5.474
Lehninger 5.448
Nozaki 5.664
DTASelect 5.804
Thurlkill 5.69
EMBOSS 5.626
Sillero 5.753
Patrickios 3.834
IPC_peptide 5.486
IPC2_peptide 5.766
IPC2.peptide.svr19 5.909
Protein with the highest isoelectric point:
>tr|A0A5Q2WA66|A0A5Q2WA66_9VIRU Replication-associated protein OS=CRESS virus sp. ctf7a5 OX=2656684 PE=4 SV=1
MM1 pKa = 7.73 EE2 pKa = 4.46 YY3 pKa = 10.68 VGGASGGVIGYY14 pKa = 9.95 ISGGPWGAYY23 pKa = 9.52 SGASLGYY30 pKa = 9.97 AAGKK34 pKa = 10.07 NFTSNSQMEE43 pKa = 4.39 RR44 pKa = 11.84 GRR46 pKa = 11.84 SRR48 pKa = 11.84 VRR50 pKa = 11.84 GLTPVRR56 pKa = 11.84 VRR58 pKa = 11.84 WGSTSRR64 pKa = 11.84 EE65 pKa = 4.17 RR66 pKa = 11.84 KK67 pKa = 8.18 WKK69 pKa = 8.82 QHH71 pKa = 4.42 NLRR74 pKa = 11.84 AISRR78 pKa = 11.84 QSRR81 pKa = 11.84 SWSRR85 pKa = 11.84 GRR87 pKa = 11.84 EE88 pKa = 3.98 LKK90 pKa = 8.83 HH91 pKa = 4.45 TRR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 LGFKK99 pKa = 10.11 KK100 pKa = 9.39 RR101 pKa = 11.84 TVRR104 pKa = 11.84 GGGLRR109 pKa = 11.84 RR110 pKa = 11.84 PVVKK114 pKa = 10.37 YY115 pKa = 9.03 PKK117 pKa = 9.3 RR118 pKa = 11.84 SKK120 pKa = 10.9 VGFKK124 pKa = 9.93 RR125 pKa = 11.84 RR126 pKa = 11.84 KK127 pKa = 8.89 IRR129 pKa = 11.84 ISKK132 pKa = 9.12 KK133 pKa = 8.47 FRR135 pKa = 11.84 RR136 pKa = 11.84 KK137 pKa = 8.77 VMKK140 pKa = 9.99 IVTGSKK146 pKa = 10.13 YY147 pKa = 10.42 KK148 pKa = 10.94 GFFMWNTYY156 pKa = 8.61 TNVTLATHH164 pKa = 6.48 KK165 pKa = 9.74 QTVHH169 pKa = 5.48 QAQCGFNVQDD179 pKa = 4.49 LQNPDD184 pKa = 3.75 LGKK187 pKa = 7.48 TTYY190 pKa = 10.32 PLFSPSVFSAVAEE203 pKa = 4.3 TLWGSKK209 pKa = 10.08 AGPLSTATSYY219 pKa = 8.86 VTGVGNNEE227 pKa = 3.78 EE228 pKa = 4.36 SNIFSFAKK236 pKa = 10.15 IPVINSWVRR245 pKa = 11.84 YY246 pKa = 8.63 CMKK249 pKa = 10.75 NNTSQVLEE257 pKa = 4.06 VKK259 pKa = 10.2 MFVCKK264 pKa = 10.25 PKK266 pKa = 10.17 VASFMYY272 pKa = 10.2 HH273 pKa = 5.12 GMNPAGQSTDD283 pKa = 3.18 LAFVYY288 pKa = 10.51 DD289 pKa = 6.01 PILDD293 pKa = 3.76 WEE295 pKa = 4.58 KK296 pKa = 11.07 AQSDD300 pKa = 4.61 LYY302 pKa = 11.19 ADD304 pKa = 3.71 GVSRR308 pKa = 11.84 YY309 pKa = 9.89 DD310 pKa = 3.98 VLLQKK315 pKa = 9.16 QTLKK319 pKa = 10.97 GRR321 pKa = 11.84 LQTDD325 pKa = 5.31 GISTMDD331 pKa = 3.19 VDD333 pKa = 4.35 PRR335 pKa = 11.84 MFTWWNKK342 pKa = 6.99 KK343 pKa = 7.34 WKK345 pKa = 10.42 ADD347 pKa = 3.9 VIKK350 pKa = 9.93 MKK352 pKa = 10.39 IMPGQCIEE360 pKa = 4.95 KK361 pKa = 9.79 IVKK364 pKa = 10.08 GPGDD368 pKa = 3.66 FVVDD372 pKa = 3.61 TFKK375 pKa = 11.03 LWRR378 pKa = 11.84 GALEE382 pKa = 4.32 NNSSRR387 pKa = 11.84 TVEE390 pKa = 4.07 NLRR393 pKa = 11.84 PAGWTFEE400 pKa = 4.75 EE401 pKa = 3.91 IQKK404 pKa = 9.28 YY405 pKa = 8.56 NRR407 pKa = 11.84 YY408 pKa = 9.36 VFWIVKK414 pKa = 9.25 PEE416 pKa = 3.74 IASVSTAVVGGVPSAIGVGRR436 pKa = 11.84 IPNPEE441 pKa = 3.93 GFTGAEE447 pKa = 4.16 CFAVEE452 pKa = 3.55 QRR454 pKa = 11.84 MYY456 pKa = 11.12 YY457 pKa = 10.57 KK458 pKa = 10.78 LALPEE463 pKa = 5.05 KK464 pKa = 8.23 MGRR467 pKa = 11.84 WNEE470 pKa = 3.67 VAYY473 pKa = 10.36 QSNDD477 pKa = 3.24 IISEE481 pKa = 3.97 NQMRR485 pKa = 11.84 DD486 pKa = 3.24 VFFCQYY492 pKa = 10.49 SKK494 pKa = 11.01 QDD496 pKa = 3.25 RR497 pKa = 11.84 ANATGSIWQNTQTAGVDD514 pKa = 2.77 IFTQIQQ520 pKa = 3.06
Molecular weight: 58.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.225
IPC2_protein 9.545
IPC_protein 9.648
Toseland 10.292
ProMoST 9.911
Dawson 10.452
Bjellqvist 10.101
Wikipedia 10.599
Rodwell 10.921
Grimsley 10.511
Solomon 10.467
Lehninger 10.438
Nozaki 10.277
DTASelect 10.087
Thurlkill 10.306
EMBOSS 10.672
Sillero 10.35
Patrickios 10.555
IPC_peptide 10.467
IPC2_peptide 8.785
IPC2.peptide.svr19 8.567
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
861
73
520
287.0
32.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.039 ± 0.798
1.045 ± 0.235
4.065 ± 0.527
5.343 ± 1.14
3.717 ± 0.752
8.246 ± 0.674
1.51 ± 0.481
4.878 ± 0.448
6.62 ± 1.205
6.852 ± 1.763
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.671 ± 0.433
4.53 ± 0.968
4.53 ± 0.421
3.949 ± 0.766
7.782 ± 0.376
6.736 ± 0.801
5.923 ± 0.198
7.549 ± 1.171
3.484 ± 0.443
4.53 ± 0.678
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here