Cellulomonas flavigena (strain ATCC 482 / DSM 20109 / BCRC 11376 / JCM 18109 / NBRC 3775 / NCIMB 8073 / NRS 134)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; Cellulomonas flavigena

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5ULG5|D5ULG5_CELFN Electron transfer flavoprotein alpha subunit OS=Cellulomonas flavigena (strain ATCC 482 / DSM 20109 / BCRC 11376 / JCM 18109 / NBRC 3775 / NCIMB 8073 / NRS 134) OX=446466 GN=Cfla_1103 PE=3 SV=1
MM1 pKa = 6.91NTRR4 pKa = 11.84SLRR7 pKa = 11.84ILTAPVLAVALAASLSSCSALDD29 pKa = 3.59SVLGNAPDD37 pKa = 3.47AVRR40 pKa = 11.84DD41 pKa = 3.72EE42 pKa = 4.61EE43 pKa = 4.5GQITEE48 pKa = 4.1AAEE51 pKa = 3.99ADD53 pKa = 4.09VFSLKK58 pKa = 10.86LGDD61 pKa = 4.25CFDD64 pKa = 4.22YY65 pKa = 11.2AAVASATEE73 pKa = 4.05ISSVSTIPCAEE84 pKa = 3.89AHH86 pKa = 6.44DD87 pKa = 4.26AEE89 pKa = 4.86IFAEE93 pKa = 4.41TTLTEE98 pKa = 4.25EE99 pKa = 3.97QFKK102 pKa = 11.28ADD104 pKa = 4.92LEE106 pKa = 4.37LTNAGDD112 pKa = 3.8TEE114 pKa = 4.67TPTFADD120 pKa = 3.92EE121 pKa = 4.15FCYY124 pKa = 10.37QQFTTFVGKK133 pKa = 10.36SYY135 pKa = 11.23EE136 pKa = 4.22EE137 pKa = 4.03STLDD141 pKa = 3.31YY142 pKa = 10.95TLFSPSEE149 pKa = 4.07EE150 pKa = 3.87GWAQGDD156 pKa = 4.07DD157 pKa = 3.62VVQCIVIHH165 pKa = 6.72PDD167 pKa = 2.91GGFTGSMKK175 pKa = 10.78DD176 pKa = 3.16SALL179 pKa = 3.64

Molecular weight: 19.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5UJL4|D5UJL4_CELFN Anaerobic ribonucleoside-triphosphate reductase activating protein OS=Cellulomonas flavigena (strain ATCC 482 / DSM 20109 / BCRC 11376 / JCM 18109 / NBRC 3775 / NCIMB 8073 / NRS 134) OX=446466 GN=Cfla_2767 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3673

0

3673

1233595

32

2585

335.9

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.704 ± 0.068

0.6 ± 0.01

6.65 ± 0.04

5.099 ± 0.037

2.323 ± 0.022

9.391 ± 0.041

2.223 ± 0.02

2.32 ± 0.03

1.215 ± 0.022

10.281 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.431 ± 0.014

1.371 ± 0.025

6.259 ± 0.036

2.627 ± 0.021

8.234 ± 0.044

4.585 ± 0.031

6.562 ± 0.046

10.759 ± 0.049

1.605 ± 0.017

1.762 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski