Mycobacterium phage Myrna

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5LJ74|B5LJ74_9CAUD Uncharacterized protein OS=Mycobacterium phage Myrna OX=546805 GN=63 PE=4 SV=1
MM1 pKa = 7.61FDD3 pKa = 2.97KK4 pKa = 10.97TFAKK8 pKa = 9.64WLSTIIAGAALVIGLSFTATSCSDD32 pKa = 3.22NGTGGQTFDD41 pKa = 2.9SLYY44 pKa = 9.96YY45 pKa = 10.66EE46 pKa = 4.52EE47 pKa = 5.54VSLPNGQRR55 pKa = 11.84VWCIGEE61 pKa = 4.23GNVGLSCDD69 pKa = 3.35WAGSRR74 pKa = 11.84DD75 pKa = 3.49

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5LJK2|B5LJK2_9CAUD DUF1508 domain-containing protein OS=Mycobacterium phage Myrna OX=546805 GN=232 PE=4 SV=1
MM1 pKa = 7.55PTTHH5 pKa = 5.95MTPAQARR12 pKa = 11.84RR13 pKa = 11.84EE14 pKa = 4.21TQALLDD20 pKa = 3.74AHH22 pKa = 6.98GLTGWKK28 pKa = 8.13ITFGTARR35 pKa = 11.84TRR37 pKa = 11.84NGSCCYY43 pKa = 8.69RR44 pKa = 11.84TRR46 pKa = 11.84TINLSRR52 pKa = 11.84TLMAFRR58 pKa = 11.84SYY60 pKa = 11.37DD61 pKa = 3.28EE62 pKa = 4.2TMDD65 pKa = 4.32TIRR68 pKa = 11.84HH69 pKa = 4.9EE70 pKa = 4.42VAHH73 pKa = 6.66ALTPGHH79 pKa = 5.84KK80 pKa = 9.34HH81 pKa = 5.73DD82 pKa = 4.57RR83 pKa = 11.84VWAAKK88 pKa = 9.86CRR90 pKa = 11.84EE91 pKa = 4.11LGGNGQRR98 pKa = 11.84CNGIADD104 pKa = 3.69EE105 pKa = 4.75AKK107 pKa = 10.33RR108 pKa = 11.84EE109 pKa = 3.95QLTQLALWVGTCEE122 pKa = 3.93HH123 pKa = 6.64GKK125 pKa = 9.5NFPRR129 pKa = 11.84HH130 pKa = 5.22RR131 pKa = 11.84QPKK134 pKa = 9.2RR135 pKa = 11.84LQGWRR140 pKa = 11.84CRR142 pKa = 11.84CSEE145 pKa = 3.85GSSPVTWRR153 pKa = 11.84KK154 pKa = 7.23TRR156 pKa = 3.26

Molecular weight: 17.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

50820

43

1296

221.9

24.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.628 ± 0.239

0.907 ± 0.064

6.834 ± 0.189

6.45 ± 0.209

3.135 ± 0.1

8.469 ± 0.307

2.21 ± 0.122

4.551 ± 0.126

4.333 ± 0.148

7.066 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.112

3.579 ± 0.112

5.63 ± 0.192

4.372 ± 0.127

6.523 ± 0.18

5.303 ± 0.149

6.415 ± 0.194

7.041 ± 0.186

1.913 ± 0.099

3.123 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski