beta proteobacterium AAP65
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3930 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N1AAP4|A0A0N1AAP4_9PROT Protein-glutamate methylesterase/protein-glutamine glutaminase OS=beta proteobacterium AAP65 OX=1523424 GN=cheB PE=3 SV=1
MM1 pKa = 7.39 ILSPEE6 pKa = 3.85 KK7 pKa = 10.27 RR8 pKa = 11.84 VNTFRR13 pKa = 11.84 TGEE16 pKa = 3.83 QTNAQVAVLADD27 pKa = 4.05 GSYY30 pKa = 10.46 VIAWQSQGQDD40 pKa = 2.92 GSGWGIYY47 pKa = 7.68 AQRR50 pKa = 11.84 YY51 pKa = 6.93 SATGAALGPEE61 pKa = 4.31 FRR63 pKa = 11.84 VSTSTTNNQTEE74 pKa = 4.49 PKK76 pKa = 9.93 LSAVPGGGFVINWTDD91 pKa = 3.77 DD92 pKa = 3.35 NGLDD96 pKa = 4.16 GSGQTVLAQRR106 pKa = 11.84 YY107 pKa = 7.11 DD108 pKa = 3.57 ANGAAQGGNVIVNTTTFSTQNDD130 pKa = 4.24 GASAGWDD137 pKa = 3.14 SGYY140 pKa = 7.37 TTVWSGYY147 pKa = 9.31 NSSTGTQDD155 pKa = 3.44 IVMQRR160 pKa = 11.84 WDD162 pKa = 3.18 AAGNRR167 pKa = 11.84 VGTEE171 pKa = 4.18 TLVGTATGAGNAAAQAGSQVAPAIASNAAGQQIMVWTDD209 pKa = 2.89 QGGSDD214 pKa = 3.23 SSGNGVYY221 pKa = 10.3 GRR223 pKa = 11.84 RR224 pKa = 11.84 YY225 pKa = 9.03 NASTNTFGDD234 pKa = 3.6 TFLITTFVAGSQYY247 pKa = 10.96 EE248 pKa = 4.1 PDD250 pKa = 3.03 VAMASDD256 pKa = 4.13 GSFVVVWRR264 pKa = 11.84 SDD266 pKa = 3.49 SQDD269 pKa = 2.83 GSGAGVFAQRR279 pKa = 11.84 FNADD283 pKa = 2.6 GSTAGTEE290 pKa = 3.99 FRR292 pKa = 11.84 VNVATDD298 pKa = 3.31 NNQYY302 pKa = 10.04 QPQVTVLSTGGFVVTWYY319 pKa = 10.3 DD320 pKa = 3.77 DD321 pKa = 3.45 SSANDD326 pKa = 3.77 GSGTSRR332 pKa = 11.84 DD333 pKa = 3.65 VYY335 pKa = 10.78 LRR337 pKa = 11.84 EE338 pKa = 3.69 YY339 pKa = 10.49 DD340 pKa = 3.39 AAGAALGGEE349 pKa = 4.4 IKK351 pKa = 10.57 LASQTSGTEE360 pKa = 3.99 SQPTIADD367 pKa = 4.22 LGAGRR372 pKa = 11.84 YY373 pKa = 9.03 LVVYY377 pKa = 10.21 TDD379 pKa = 3.99 DD380 pKa = 4.1 TPDD383 pKa = 3.61 GGSDD387 pKa = 2.64 IVQQIFGPDD396 pKa = 4.19 FIGPIQQGGPAIGDD410 pKa = 3.49 WEE412 pKa = 4.45 GSITFAEE419 pKa = 4.39 NTVNAAPQLIDD430 pKa = 3.35 IVVALTDD437 pKa = 4.23 LDD439 pKa = 4.07 SANLDD444 pKa = 3.49 GGRR447 pKa = 11.84 LDD449 pKa = 4.24 LYY451 pKa = 10.31 YY452 pKa = 11.07 VQGGSTADD460 pKa = 3.37 QLGVRR465 pKa = 11.84 SGGTGPNQISVSGANVLFEE484 pKa = 3.94 GVVFGTLSGGANGSGLSVVFNANATVDD511 pKa = 3.59 AVEE514 pKa = 4.72 EE515 pKa = 4.69 LIQQLTYY522 pKa = 11.37 ANTSQSPAASRR533 pKa = 11.84 VVGLRR538 pKa = 11.84 VSDD541 pKa = 3.84 GDD543 pKa = 3.9 GGASPGYY550 pKa = 10.64 AKK552 pKa = 10.18 IIQVTPEE559 pKa = 3.94 ADD561 pKa = 3.47 GEE563 pKa = 4.16 LALYY567 pKa = 10.61 GEE569 pKa = 4.64 STVNSFVAGEE579 pKa = 3.87 QRR581 pKa = 11.84 APAVATLADD590 pKa = 3.57 GGYY593 pKa = 9.37 VVVWQSSLQVDD604 pKa = 3.42 NTYY607 pKa = 10.9 EE608 pKa = 3.87 IFFQRR613 pKa = 11.84 FNASGLAVGPEE624 pKa = 3.8 QRR626 pKa = 11.84 VNTVTSSTQEE636 pKa = 3.99 LPHH639 pKa = 6.52 VAGLSNGGFVITWDD653 pKa = 4.07 DD654 pKa = 3.31 ASGSDD659 pKa = 3.49 GSGRR663 pKa = 11.84 GVYY666 pKa = 8.12 GQRR669 pKa = 11.84 YY670 pKa = 8.97 DD671 pKa = 3.48 SQGQALGNNFLANTFTSSTQQYY693 pKa = 10.38 NDD695 pKa = 3.07 VASWAGAGPVAAGYY709 pKa = 10.33 AVVWASSGNNPDD721 pKa = 3.14 SSGWEE726 pKa = 3.58 IYY728 pKa = 9.7 LQRR731 pKa = 11.84 FDD733 pKa = 3.68 NAGNKK738 pKa = 9.44 VGGEE742 pKa = 4.08 LRR744 pKa = 11.84 VSDD747 pKa = 4.16 AVGASTAQPGTQYY760 pKa = 10.44 EE761 pKa = 4.22 PSIAAHH767 pKa = 6.45 ANGDD771 pKa = 4.05 LVIAWRR777 pKa = 11.84 DD778 pKa = 3.3 DD779 pKa = 3.46 SGNDD783 pKa = 3.56 GSGVGVFGRR792 pKa = 11.84 QFNAATDD799 pKa = 3.78 SFGPTFLISTTTAGSQYY816 pKa = 10.13 EE817 pKa = 4.2 VDD819 pKa = 3.73 VATLSDD825 pKa = 3.61 GGFVAVWRR833 pKa = 11.84 SDD835 pKa = 3.52 GQDD838 pKa = 3.03 GSSAGVYY845 pKa = 8.15 GQRR848 pKa = 11.84 FDD850 pKa = 3.63 AAGAKK855 pKa = 10.06 VGGEE859 pKa = 3.93 FIVNEE864 pKa = 4.33 TTSGGQYY871 pKa = 8.11 QPKK874 pKa = 10.38 VIGLSTGGFVVSFYY888 pKa = 11.18 NDD890 pKa = 3.04 NYY892 pKa = 11.16 DD893 pKa = 3.4 FTGTGTQNDD902 pKa = 4.32 VYY904 pKa = 10.68 IRR906 pKa = 11.84 QYY908 pKa = 10.62 DD909 pKa = 3.77 ANGVAVDD916 pKa = 3.91 SQRR919 pKa = 11.84 KK920 pKa = 8.46 LLSPTNNTEE929 pKa = 4.03 FEE931 pKa = 4.17 PALADD936 pKa = 3.76 LGNGNFVVVYY946 pKa = 10.26 ADD948 pKa = 3.7 YY949 pKa = 8.86 NTGVPGANSTYY960 pKa = 10.85 NIVQQLFGNAAEE972 pKa = 4.86 VPRR975 pKa = 11.84 QANPSLGDD983 pKa = 3.33 FTGSVTFAEE992 pKa = 4.35 NLVNATPQVIDD1003 pKa = 3.92 GAVSLNDD1010 pKa = 3.16 IDD1012 pKa = 4.62 SANFDD1017 pKa = 3.64 GGRR1020 pKa = 11.84 LDD1022 pKa = 4.15 LYY1024 pKa = 10.51 YY1025 pKa = 11.08 VQGGEE1030 pKa = 4.0 NTDD1033 pKa = 3.41 QFGVRR1038 pKa = 11.84 HH1039 pKa = 5.75 VGNGAGQIGVAGSTISYY1056 pKa = 9.64 GGVAIGSISGLGTGGQGNNLRR1077 pKa = 11.84 IDD1079 pKa = 4.13 FTAGATVEE1087 pKa = 4.27 AVQALVQNLTYY1098 pKa = 10.91 ASTSQSPAAEE1108 pKa = 3.8 RR1109 pKa = 11.84 TVGLRR1114 pKa = 11.84 LSDD1117 pKa = 3.79 GDD1119 pKa = 4.18 GGSTDD1124 pKa = 3.73 ASLITINVTRR1134 pKa = 11.84 EE1135 pKa = 3.78 LDD1137 pKa = 3.69 GQPLVWGEE1145 pKa = 4.12 EE1146 pKa = 3.98 AVNTYY1151 pKa = 8.82 TVDD1154 pKa = 3.44 AQDD1157 pKa = 3.97 DD1158 pKa = 4.15 PEE1160 pKa = 5.66 IGVLADD1166 pKa = 3.57 GGYY1169 pKa = 10.07 VIVWTSAGQDD1179 pKa = 3.45 GQADD1183 pKa = 4.01 GVYY1186 pKa = 7.87 MQRR1189 pKa = 11.84 YY1190 pKa = 8.08 NNVGVAVGGEE1200 pKa = 3.74 IRR1202 pKa = 11.84 VNSLTVSQQNYY1213 pKa = 8.02 AHH1215 pKa = 6.37 VAGLTGAGGGGWVVTWQDD1233 pKa = 2.99 SSNDD1237 pKa = 3.25 GSGWGVYY1244 pKa = 7.11 GQRR1247 pKa = 11.84 YY1248 pKa = 8.78 DD1249 pKa = 3.53 ADD1251 pKa = 3.75 GVAVGGQFLVNSVSTGTQYY1270 pKa = 11.46 HH1271 pKa = 6.96 DD1272 pKa = 4.06 AVASYY1277 pKa = 10.84 DD1278 pKa = 3.17 GGYY1281 pKa = 10.36 AVVWASSQAGGNAFDD1296 pKa = 4.9 IYY1298 pKa = 10.84 LKK1300 pKa = 10.55 RR1301 pKa = 11.84 YY1302 pKa = 9.59 DD1303 pKa = 3.51 NTGAITTAEE1312 pKa = 4.07 TRR1314 pKa = 11.84 VSVVAASAQTGTQEE1328 pKa = 4.29 LPDD1331 pKa = 3.41 VAAYY1335 pKa = 10.71 ANGNLVIVWQDD1346 pKa = 2.93 ANGNDD1351 pKa = 3.77 GASYY1355 pKa = 10.89 GVFARR1360 pKa = 11.84 LYY1362 pKa = 10.28 DD1363 pKa = 3.62 ATTGTFGDD1371 pKa = 3.84 TFLVNGTTTGNQYY1384 pKa = 10.06 EE1385 pKa = 4.29 PSVAVLSDD1393 pKa = 3.18 GGFVVTWRR1401 pKa = 11.84 SDD1403 pKa = 3.63 NQDD1406 pKa = 2.96 GASAGVYY1413 pKa = 9.88 AQVFTAAGAKK1423 pKa = 9.97 VGGEE1427 pKa = 3.79 FLVNEE1432 pKa = 4.44 TTAGGQYY1439 pKa = 9.37 QAAVTGLSGGGFAVSWYY1456 pKa = 10.46 SDD1458 pKa = 3.08 TSDD1461 pKa = 3.98 AGTSSDD1467 pKa = 3.51 VFVRR1471 pKa = 11.84 EE1472 pKa = 3.92 FTNAGVPIDD1481 pKa = 4.08 GEE1483 pKa = 4.36 RR1484 pKa = 11.84 KK1485 pKa = 9.57 LLSATNSTEE1494 pKa = 3.81 QQPAIAGLASGGFVVAYY1511 pKa = 10.11 ADD1513 pKa = 3.74 YY1514 pKa = 9.71 VTTAGGGNNTYY1525 pKa = 10.96 GIAQQLFGNPAALARR1540 pKa = 11.84 QANPVLGDD1548 pKa = 3.26 FTGSVTFAEE1557 pKa = 4.35 NLVNATPQLIDD1568 pKa = 3.33 AAVALVDD1575 pKa = 3.61 TDD1577 pKa = 3.67 SANFDD1582 pKa = 3.7 GGRR1585 pKa = 11.84 LDD1587 pKa = 3.79 LFYY1590 pKa = 11.32 VQGGEE1595 pKa = 4.61 AGDD1598 pKa = 3.59 QLGVRR1603 pKa = 11.84 NVGNAAGQIGVAGGTISFGGVAIGTVAAALTGSNGSALRR1642 pKa = 11.84 IDD1644 pKa = 4.59 LNANASTEE1652 pKa = 4.03 AVEE1655 pKa = 5.56 ALVQSLTYY1663 pKa = 10.59 GSTSEE1668 pKa = 4.62 GPALSRR1674 pKa = 11.84 TVGLRR1679 pKa = 11.84 LTDD1682 pKa = 3.65 GDD1684 pKa = 4.25 GGSTAPAAVTINVTPNLDD1702 pKa = 3.83 GAPLVYY1708 pKa = 10.41 GEE1710 pKa = 4.85 SLGSVNTYY1718 pKa = 10.24 RR1719 pKa = 11.84 EE1720 pKa = 4.23 STQEE1724 pKa = 3.91 YY1725 pKa = 10.04 PEE1727 pKa = 4.29 VAVLADD1733 pKa = 3.72 GSYY1736 pKa = 10.17 VVTWTSSGQDD1746 pKa = 3.24 GSGNGVYY1753 pKa = 10.16 AQRR1756 pKa = 11.84 YY1757 pKa = 5.23 TGAGVAIGNEE1767 pKa = 4.02 VRR1769 pKa = 11.84 VNTATSSTQDD1779 pKa = 3.17 FSQITALSDD1788 pKa = 3.0 GGYY1791 pKa = 10.14 VIVWQDD1797 pKa = 2.76 ISGSDD1802 pKa = 3.49 GAGWGVIGQRR1812 pKa = 11.84 YY1813 pKa = 9.04 DD1814 pKa = 3.02 SSGAAVGSNFTVNTTTAGSQYY1835 pKa = 9.6 HH1836 pKa = 5.79 GVVAGYY1842 pKa = 8.33 TGGFAAVWSGSQGAAGFSGQDD1863 pKa = 2.56 IYY1865 pKa = 11.58 LRR1867 pKa = 11.84 RR1868 pKa = 11.84 FDD1870 pKa = 3.94 NNGVQVTAEE1879 pKa = 3.83 LRR1881 pKa = 11.84 VNNTSGTTAQTSTQEE1896 pKa = 4.21 LPDD1899 pKa = 3.31 VAAQANGDD1907 pKa = 4.46 LLIVWQDD1914 pKa = 3.31 QAGLDD1919 pKa = 3.79 GNAWGVFGRR1928 pKa = 11.84 FYY1930 pKa = 11.33 DD1931 pKa = 3.69 AATGLLGPLFTVNTQTADD1949 pKa = 3.33 SQYY1952 pKa = 9.06 EE1953 pKa = 3.88 PSVAALSNGGFVVVWRR1969 pKa = 11.84 SNGGQDD1975 pKa = 2.93 GDD1977 pKa = 3.75 AAGVFGQLLDD1987 pKa = 3.55 ATGAKK1992 pKa = 10.04 VGGEE1996 pKa = 3.69 FLVNQRR2002 pKa = 11.84 TPGSQYY2008 pKa = 10.66 QPDD2011 pKa = 3.82 VTGLSTGGFVVTWYY2025 pKa = 11.0 NDD2027 pKa = 3.29 NTDD2030 pKa = 3.03 VSGTGTQNDD2039 pKa = 3.9 VYY2041 pKa = 10.83 IRR2043 pKa = 11.84 EE2044 pKa = 4.09 FNNAGVALDD2053 pKa = 4.33 GEE2055 pKa = 4.69 RR2056 pKa = 11.84 KK2057 pKa = 9.85 LPSLTNSTEE2066 pKa = 3.97 YY2067 pKa = 10.77 QPAIADD2073 pKa = 3.8 LGNGNFVVAYY2083 pKa = 9.55 SDD2085 pKa = 4.3 YY2086 pKa = 11.18 NATPINGAGGNTWEE2100 pKa = 4.77 IRR2102 pKa = 11.84 TQIFGDD2108 pKa = 3.54 AAEE2111 pKa = 4.48 LARR2114 pKa = 11.84 PSANPVLGDD2123 pKa = 3.39 VRR2125 pKa = 11.84 LLRR2128 pKa = 11.84 TLTNAPADD2136 pKa = 3.67 ANFYY2140 pKa = 10.89 AGTARR2145 pKa = 11.84 VIDD2148 pKa = 3.82 TDD2150 pKa = 3.69 ISVTDD2155 pKa = 3.55 SDD2157 pKa = 4.25 SANFDD2162 pKa = 3.45 GGQLTVFFTAGLSANDD2178 pKa = 3.67 TLGLRR2183 pKa = 11.84 NEE2185 pKa = 4.53 GNGAGQVGVSGSDD2198 pKa = 3.18 VSFGGTVVGSYY2209 pKa = 9.75 TGGGAGAPNLVVTFNANATAEE2230 pKa = 4.22 AVRR2233 pKa = 11.84 AVGEE2237 pKa = 4.31 NITYY2241 pKa = 10.58 LHH2243 pKa = 6.3 TAPPAGTTDD2252 pKa = 2.94 RR2253 pKa = 11.84 HH2254 pKa = 6.06 IGIRR2258 pKa = 11.84 LTDD2261 pKa = 3.81 GDD2263 pKa = 4.56 GGASDD2268 pKa = 4.32 ASTVQIRR2275 pKa = 11.84 IQASFSAPVLGLDD2288 pKa = 4.81 DD2289 pKa = 4.03 VASTLALTEE2298 pKa = 4.16 TQALAGALIDD2308 pKa = 4.42 PDD2310 pKa = 3.74 VQLSYY2315 pKa = 10.68 TGSNWANGKK2324 pKa = 8.48 LTVTYY2329 pKa = 10.37 LSSTNRR2335 pKa = 11.84 SDD2337 pKa = 3.42 DD2338 pKa = 3.26 QLAIRR2343 pKa = 11.84 NEE2345 pKa = 3.95 GSAAGQVGVVGSDD2358 pKa = 3.21 VLFGGVSIGTISATLNGANGSTLDD2382 pKa = 3.74 ITWKK2386 pKa = 9.35 ATATEE2391 pKa = 4.18 TAIEE2395 pKa = 3.91 RR2396 pKa = 11.84 VIEE2399 pKa = 3.9 NLRR2402 pKa = 11.84 YY2403 pKa = 9.93 SSTSSEE2409 pKa = 4.03 PLASRR2414 pKa = 11.84 SVRR2417 pKa = 11.84 ISLNDD2422 pKa = 3.7 GAGNAATARR2431 pKa = 11.84 DD2432 pKa = 3.71 VSITVAAEE2440 pKa = 3.73 DD2441 pKa = 4.46 DD2442 pKa = 4.15 ANAVQPLLGTEE2453 pKa = 4.02 QQVNTFEE2460 pKa = 5.83 SNQQQNPAVARR2471 pKa = 11.84 LLGSNDD2477 pKa = 2.53 GSYY2480 pKa = 10.67 VVTWTSVGAQDD2491 pKa = 5.28 GDD2493 pKa = 3.67 DD2494 pKa = 3.86 HH2495 pKa = 8.01 GIFAQRR2501 pKa = 11.84 YY2502 pKa = 7.28 ASNGAAIGPEE2512 pKa = 4.04 FQVNTTTLNSQTEE2525 pKa = 4.03 PAIGGLAGGGFVIAWRR2541 pKa = 11.84 ATGQDD2546 pKa = 2.98 GGGSYY2551 pKa = 10.74 GVYY2554 pKa = 9.25 AQRR2557 pKa = 11.84 YY2558 pKa = 7.02 NADD2561 pKa = 3.53 GSPAGPEE2568 pKa = 3.91 FRR2570 pKa = 11.84 ATTSTVGNQYY2580 pKa = 10.42 EE2581 pKa = 4.22 PAVLGLSDD2589 pKa = 3.76 GSFIIAYY2596 pKa = 10.03 RR2597 pKa = 11.84 SDD2599 pKa = 3.46 YY2600 pKa = 10.24 TGNSSLNDD2608 pKa = 3.09 VLAQRR2613 pKa = 11.84 FAADD2617 pKa = 3.68 GSLLGSEE2624 pKa = 4.2 ITLNTAGGATQYY2636 pKa = 10.7 QPQLAALSGGGFVAVWTDD2654 pKa = 3.06 TSGDD2658 pKa = 3.43 ASGSGVVAQLFTNAGAKK2675 pKa = 9.55 VGSPISINTTTAGAQSGVHH2694 pKa = 5.14 VAALSGGGFVAVWDD2708 pKa = 4.62 DD2709 pKa = 4.01 GNDD2712 pKa = 3.11 VRR2714 pKa = 11.84 AQRR2717 pKa = 11.84 FDD2719 pKa = 3.29 AAGTPVGTEE2728 pKa = 3.67 LRR2730 pKa = 11.84 VNGSDD2735 pKa = 4.41 SPSNLNGEE2743 pKa = 4.25 PRR2745 pKa = 11.84 VAALDD2750 pKa = 3.65 NNGFIVTWDD2759 pKa = 3.9 GNPSAALGGNSWDD2772 pKa = 4.07 VMTQQFDD2779 pKa = 3.33 AAGNKK2784 pKa = 9.05 VDD2786 pKa = 4.65 GVQVLNTVRR2795 pKa = 11.84 SSTQYY2800 pKa = 9.36 EE2801 pKa = 4.11 PEE2803 pKa = 3.88 IVGLGGNKK2811 pKa = 9.79 AVVVWAGYY2819 pKa = 8.12 DD2820 pKa = 3.46 QEE2822 pKa = 5.22 RR2823 pKa = 11.84 GTVNTYY2829 pKa = 10.66 GVFQQVLGAPGSLTGSAAPVLTDD2852 pKa = 3.92 LAASVTFAEE2861 pKa = 4.39 NLVNSAPQLIDD2872 pKa = 3.4 AGIGLVDD2879 pKa = 3.47 TDD2881 pKa = 3.97 SANFDD2886 pKa = 3.91 GGTLWVSVISGYY2898 pKa = 11.33 GNIEE2902 pKa = 3.93 TAQLADD2908 pKa = 3.86 PAAQQDD2914 pKa = 3.73 QFGIRR2919 pKa = 11.84 HH2920 pKa = 5.6 QGNGPGQVGVAGNTVSVGGVAIGTITGTGVNGADD2954 pKa = 3.41 LVVSFNANATASLVEE2969 pKa = 4.2 ALLEE2973 pKa = 4.12 NLTYY2977 pKa = 11.17 ANTASAPVPARR2988 pKa = 11.84 TVSISLSDD2996 pKa = 3.64 GDD2998 pKa = 4.56 GGTSVPRR3005 pKa = 11.84 AVVINITPEE3014 pKa = 3.94 ADD3016 pKa = 3.16 GGSPLFGNEE3025 pKa = 3.8 RR3026 pKa = 11.84 VNTYY3030 pKa = 10.86 APSTQDD3036 pKa = 3.0 NPASARR3042 pKa = 11.84 LSDD3045 pKa = 3.38 GGYY3048 pKa = 6.76 ITVWEE3053 pKa = 4.43 SSGQDD3058 pKa = 2.79 GWNEE3062 pKa = 3.32 GVYY3065 pKa = 10.33 AQRR3068 pKa = 11.84 FDD3070 pKa = 3.68 AQGVAVGAEE3079 pKa = 3.84 FRR3081 pKa = 11.84 INSNTRR3087 pKa = 11.84 DD3088 pKa = 3.64 SQYY3091 pKa = 10.69 EE3092 pKa = 3.84 PAVAGLNGGGYY3103 pKa = 9.39 VVTWRR3108 pKa = 11.84 SNGQDD3113 pKa = 3.14 GSGTGVYY3120 pKa = 9.82 AQRR3123 pKa = 11.84 FANDD3127 pKa = 3.29 GTALGGEE3134 pKa = 4.31 FLVNTSTDD3142 pKa = 3.23 QNQYY3146 pKa = 10.33 QPTIAATADD3155 pKa = 3.08 GGFVIGWYY3163 pKa = 10.02 DD3164 pKa = 4.6 DD3165 pKa = 4.2 YY3166 pKa = 11.47 YY3167 pKa = 10.67 TDD3169 pKa = 5.14 AGFTEE3174 pKa = 4.15 YY3175 pKa = 10.81 RR3176 pKa = 11.84 DD3177 pKa = 3.63 VFFQRR3182 pKa = 11.84 YY3183 pKa = 6.4 NAAGVAQGGEE3193 pKa = 4.2 TRR3195 pKa = 11.84 ANPAGEE3201 pKa = 4.39 GTVSQYY3207 pKa = 11.45 EE3208 pKa = 4.09 PVLVGLSGGGFVMMWTDD3225 pKa = 3.47 DD3226 pKa = 3.9 SKK3228 pKa = 11.59 DD3229 pKa = 3.56 GSGTGVFGQRR3239 pKa = 11.84 FDD3241 pKa = 3.75 AAGSALGASFRR3252 pKa = 11.84 VNSYY3256 pKa = 6.6 TTNAQSQAAAAALNDD3271 pKa = 4.33 GGWIAVWSSEE3281 pKa = 3.9 GQDD3284 pKa = 3.45 GSGLGVFGQRR3294 pKa = 11.84 YY3295 pKa = 8.3 AADD3298 pKa = 3.95 GSLVGGEE3305 pKa = 3.95 FRR3307 pKa = 11.84 VNTNTASTQYY3317 pKa = 10.21 LPSVTGLSHH3326 pKa = 6.79 GGFAVSYY3333 pKa = 10.1 YY3334 pKa = 10.75 SGGNQWVQAYY3344 pKa = 7.98 DD3345 pKa = 3.33 ATGARR3350 pKa = 11.84 LDD3352 pKa = 3.59 GEE3354 pKa = 4.39 QRR3356 pKa = 11.84 VDD3358 pKa = 3.86 ALDD3361 pKa = 3.74 NNGNSNASSIVALANGGFVVTFRR3384 pKa = 11.84 DD3385 pKa = 3.12 YY3386 pKa = 11.29 DD3387 pKa = 4.03 YY3388 pKa = 11.81 NDD3390 pKa = 3.2 GSTYY3394 pKa = 11.09 NVYY3397 pKa = 10.0 QQLFGKK3403 pKa = 9.27 PADD3406 pKa = 3.97 FTRR3409 pKa = 11.84 QANPQLVDD3417 pKa = 3.23 VATSVTFDD3425 pKa = 3.69 EE3426 pKa = 5.17 DD3427 pKa = 3.97 LVNATPQLIDD3437 pKa = 3.7 PVVGLSDD3444 pKa = 4.05 ADD3446 pKa = 3.64 STNFDD3451 pKa = 3.55 GGVLEE3456 pKa = 4.35 VNYY3459 pKa = 9.17 LTGYY3463 pKa = 10.5 ALQDD3467 pKa = 3.38 QLGLSGLSAQDD3478 pKa = 3.28 QLGVRR3483 pKa = 11.84 HH3484 pKa = 6.3 EE3485 pKa = 4.44 GSGVGQVGVAGNTVSFGGVAIGTITSNGVNGSKK3518 pKa = 10.46 LAISFNANASVVAVEE3533 pKa = 4.61 RR3534 pKa = 11.84 VIEE3537 pKa = 4.16 SLTYY3541 pKa = 11.09 ANTFSDD3547 pKa = 4.03 PAATRR3552 pKa = 11.84 TVSIRR3557 pKa = 11.84 VTDD3560 pKa = 3.92 GDD3562 pKa = 3.71 GGASVARR3569 pKa = 11.84 SVEE3572 pKa = 4.19 IKK3574 pKa = 9.33 VTPQFDD3580 pKa = 3.57 GAQPVFDD3587 pKa = 3.84 NEE3589 pKa = 4.33 RR3590 pKa = 11.84 VNSYY3594 pKa = 10.7 RR3595 pKa = 11.84 PNTQSDD3601 pKa = 3.42 AAMASLADD3609 pKa = 3.78 GSYY3612 pKa = 8.39 ITVWTSATAQDD3623 pKa = 3.37 GWDD3626 pKa = 3.28 AAVYY3630 pKa = 9.51 AQRR3633 pKa = 11.84 FAPNGVPLGTEE3644 pKa = 4.13 FQVNTYY3650 pKa = 8.63 TPYY3653 pKa = 9.48 SQSDD3657 pKa = 3.53 ATVVGLSGGGFVIAWRR3673 pKa = 11.84 SDD3675 pKa = 3.36 NQDD3678 pKa = 2.67 SSGAGVYY3685 pKa = 8.9 AQRR3688 pKa = 11.84 YY3689 pKa = 8.38 DD3690 pKa = 3.41 SAGRR3694 pKa = 11.84 PQGAEE3699 pKa = 3.63 FRR3701 pKa = 11.84 VNTSTDD3707 pKa = 3.07 QNQYY3711 pKa = 9.83 QPSIAATADD3720 pKa = 3.19 GGFVIAWYY3728 pKa = 10.22 DD3729 pKa = 3.51 DD3730 pKa = 4.09 YY3731 pKa = 11.16 YY3732 pKa = 10.93 TNAGFTEE3739 pKa = 3.93 YY3740 pKa = 10.79 RR3741 pKa = 11.84 DD3742 pKa = 3.54 IFFQRR3747 pKa = 11.84 YY3748 pKa = 6.35 NAAGVAQGGEE3758 pKa = 4.2 TRR3760 pKa = 11.84 ANPAGLGTVSQYY3772 pKa = 10.93 EE3773 pKa = 3.87 PSITGLVGGGFVVTWSDD3790 pKa = 4.02 DD3791 pKa = 3.84 GADD3794 pKa = 3.86 GNSNGINGQRR3804 pKa = 11.84 FDD3806 pKa = 4.33 ASGNAQGASFRR3817 pKa = 11.84 VNTYY3821 pKa = 6.98 TTSSQDD3827 pKa = 3.31 NSVVTALNDD3836 pKa = 3.61 GGFLVTWRR3844 pKa = 11.84 SEE3846 pKa = 4.24 GQDD3849 pKa = 3.37 SSSGGIFSQRR3859 pKa = 11.84 YY3860 pKa = 7.84 AADD3863 pKa = 4.15 GSPVGAEE3870 pKa = 3.61 FRR3872 pKa = 11.84 VNTTVSGNQFDD3883 pKa = 4.12 AAVAALSTGGWVVAWEE3899 pKa = 4.13 SSGQIFVQQFSATGEE3914 pKa = 4.18 RR3915 pKa = 11.84 VDD3917 pKa = 5.08 GEE3919 pKa = 4.09 IKK3921 pKa = 10.75 ADD3923 pKa = 4.12 LFPNASNYY3931 pKa = 10.36 AGQPALAALPDD3942 pKa = 3.69 GGFVVSFYY3950 pKa = 11.04 DD3951 pKa = 3.85 YY3952 pKa = 11.65 DD3953 pKa = 3.83 NTTNEE3958 pKa = 3.9 YY3959 pKa = 10.77 DD3960 pKa = 3.51 VYY3962 pKa = 10.85 QQVFGNPATLVRR3974 pKa = 11.84 QANPLLVDD3982 pKa = 3.83 VNSSVSFGEE3991 pKa = 3.87 NLVNAVPQLIDD4002 pKa = 3.83 AGVGLQDD4009 pKa = 3.08 VDD4011 pKa = 4.19 SPNFDD4016 pKa = 3.36 GGLLEE4021 pKa = 4.45 VNYY4024 pKa = 9.11 LTGYY4028 pKa = 9.15 GAQDD4032 pKa = 3.19 QLGQQGLDD4040 pKa = 3.36 SQDD4043 pKa = 3.27 QLGIRR4048 pKa = 11.84 SEE4050 pKa = 4.44 GTGARR4055 pKa = 11.84 QVSVAGAVVSYY4066 pKa = 10.46 QGVAIGTIVSNGANGNKK4083 pKa = 8.68 LTVQFNANATVDD4095 pKa = 3.44 AVEE4098 pKa = 4.02 QVIEE4102 pKa = 4.2 SLTYY4106 pKa = 11.02 ANTVSNPIANRR4117 pKa = 11.84 TISIRR4122 pKa = 11.84 VTDD4125 pKa = 4.02 GDD4127 pKa = 3.88 GGASEE4132 pKa = 4.61 ARR4134 pKa = 11.84 TVNINVRR4141 pKa = 11.84 PEE4143 pKa = 3.31 TDD4145 pKa = 3.24 GAVKK4149 pKa = 10.44 FGLEE4153 pKa = 4.03 KK4154 pKa = 10.84 VVNTTVAGNQVEE4166 pKa = 4.33 PAVARR4171 pKa = 11.84 VSDD4174 pKa = 3.57 GGYY4177 pKa = 9.17 VVVWTDD4183 pKa = 2.88 QGGADD4188 pKa = 3.53 GSSWGVYY4195 pKa = 6.83 GQRR4198 pKa = 11.84 YY4199 pKa = 9.96 DD4200 pKa = 3.76 SDD4202 pKa = 4.24 DD4203 pKa = 3.73 NAVGGQFRR4211 pKa = 11.84 VNTVTTNAQYY4221 pKa = 10.14 EE4222 pKa = 4.22 AQITALTGGGWVVTWRR4238 pKa = 11.84 SDD4240 pKa = 3.5 SQDD4243 pKa = 2.83 GSGAGVYY4250 pKa = 7.78 GQRR4253 pKa = 11.84 YY4254 pKa = 8.14 AADD4257 pKa = 3.88 GSTAGTEE4264 pKa = 4.14 FRR4266 pKa = 11.84 VNTGTDD4272 pKa = 3.12 QNQYY4276 pKa = 9.99 QPSITATADD4285 pKa = 2.98 GGFVVAWYY4293 pKa = 10.25 DD4294 pKa = 4.65 DD4295 pKa = 4.32 YY4296 pKa = 11.67 YY4297 pKa = 10.91 TDD4299 pKa = 5.14 AGFTEE4304 pKa = 4.23 YY4305 pKa = 10.81 RR4306 pKa = 11.84 DD4307 pKa = 3.75 VLFQRR4312 pKa = 11.84 YY4313 pKa = 9.14 DD4314 pKa = 3.35 SNGVVQGVEE4323 pKa = 4.1 TRR4325 pKa = 11.84 ANPSGLGTTTQNEE4338 pKa = 3.92 PSIAGLAGGGFVVIWTDD4355 pKa = 3.51 HH4356 pKa = 5.89 SADD4359 pKa = 3.75 GNGLGVFGQRR4369 pKa = 11.84 FSAAGVAEE4377 pKa = 4.43 GAAFAVNSYY4386 pKa = 10.86 VDD4388 pKa = 3.92 STQSAASVAALKK4400 pKa = 10.94 DD4401 pKa = 3.3 GGFVVTWEE4409 pKa = 4.37 SAGQDD4414 pKa = 3.46 LSSTGIYY4421 pKa = 9.58 AQRR4424 pKa = 11.84 FDD4426 pKa = 3.51 ATGTKK4431 pKa = 10.21 LGAEE4435 pKa = 4.09 FRR4437 pKa = 11.84 VNTNINGQQYY4447 pKa = 9.44 EE4448 pKa = 4.27 PAVTALEE4455 pKa = 4.19 SGGWVITWTDD4465 pKa = 3.78 TNVGSYY4471 pKa = 10.61 DD4472 pKa = 3.57 VYY4474 pKa = 11.03 AQQYY4478 pKa = 8.45 DD4479 pKa = 3.46 AAGRR4483 pKa = 11.84 AVDD4486 pKa = 5.25 GEE4488 pKa = 4.62 TLVNSYY4494 pKa = 10.32 RR4495 pKa = 11.84 YY4496 pKa = 7.9 STQWQPAVASMADD4509 pKa = 3.11 GGFVVTYY4516 pKa = 10.0 AGYY4519 pKa = 10.25 IATGDD4524 pKa = 3.84 GNGDD4528 pKa = 4.59 GIPDD4532 pKa = 4.43 GGNDD4536 pKa = 3.24 TYY4538 pKa = 10.86 EE4539 pKa = 3.67 IRR4541 pKa = 11.84 LQRR4544 pKa = 11.84 FSNTAPNIQNVTVTGAEE4561 pKa = 3.87 EE4562 pKa = 4.13 AVITLSNTLFEE4573 pKa = 5.65 GGFTDD4578 pKa = 5.16 PDD4580 pKa = 4.04 GQSLQAIKK4588 pKa = 10.37 IVTLPAEE4595 pKa = 4.25 GTLRR4599 pKa = 11.84 LDD4601 pKa = 3.5 GTPVLSGQEE4610 pKa = 3.61 ISIEE4614 pKa = 3.84 QLQAGLLTYY4623 pKa = 10.2 QGNVDD4628 pKa = 3.74 YY4629 pKa = 10.89 FGNDD4633 pKa = 2.93 QFRR4636 pKa = 11.84 WTGSDD4641 pKa = 3.14 GVAFASTPVFTNIVLSNVNDD4661 pKa = 4.35 GPRR4664 pKa = 11.84 LGLLTDD4670 pKa = 3.92 AAGSEE4675 pKa = 4.31 GSVFSRR4681 pKa = 11.84 SITIGDD4687 pKa = 4.44 PDD4689 pKa = 4.31 PEE4691 pKa = 4.36 SHH4693 pKa = 5.94 QVTVNWGDD4701 pKa = 3.39 GSANTVFVTSLANPNISHH4719 pKa = 6.89 VYY4721 pKa = 10.05 ADD4723 pKa = 3.5 NGNYY4727 pKa = 8.36 TVTVTVNDD4735 pKa = 3.45 QQGQGNSIEE4744 pKa = 4.59 SGSFTANIANIAPTIPLFGNTTVEE4768 pKa = 3.86 QGQVYY4773 pKa = 8.14 TLDD4776 pKa = 3.83 LGSVNDD4782 pKa = 4.91 PGDD4785 pKa = 4.09 DD4786 pKa = 3.52 DD4787 pKa = 3.84 VTEE4790 pKa = 4.05 YY4791 pKa = 10.74 RR4792 pKa = 11.84 INWGDD4797 pKa = 3.51 GSPVEE4802 pKa = 4.49 TYY4804 pKa = 8.93 TAATLPPGGILTHH4817 pKa = 6.15 TFVTVGPKK4825 pKa = 9.78 TIVVGLTDD4833 pKa = 3.65 EE4834 pKa = 4.97 DD4835 pKa = 3.84 GVYY4838 pKa = 11.12 ANAGTKK4844 pKa = 9.69 TIDD4847 pKa = 3.11 VSAPAEE4853 pKa = 4.4 VITVNAGADD4862 pKa = 3.44 TTVNEE4867 pKa = 4.42 GNFFSRR4873 pKa = 11.84 TIFFDD4878 pKa = 4.12 DD4879 pKa = 4.1 PTDD4882 pKa = 3.24 QGAAGRR4888 pKa = 11.84 TYY4890 pKa = 10.48 TVDD4893 pKa = 2.82 WGDD4896 pKa = 3.67 GQTSNGNIAAGQFSFNIGHH4915 pKa = 6.5 SYY4917 pKa = 10.64 ADD4919 pKa = 3.14 NGGPYY4924 pKa = 9.15 TVTVTVDD4931 pKa = 3.17 DD4932 pKa = 4.31 SGVVQQVGSDD4942 pKa = 3.57 SFIVNVNNVAPQIFIGGSSAVAEE4965 pKa = 4.38 GSVYY4969 pKa = 10.95 TMTLTRR4975 pKa = 11.84 SDD4977 pKa = 4.09 PGADD4981 pKa = 3.39 TISEE4985 pKa = 4.19 YY4986 pKa = 11.22 VIDD4989 pKa = 4.01 WGDD4992 pKa = 3.48 GTVQTVLAVDD5002 pKa = 5.08 FPANGQFNHH5011 pKa = 6.96 TYY5013 pKa = 10.77 DD5014 pKa = 4.34 DD5015 pKa = 4.27 GNITRR5020 pKa = 11.84 QIRR5023 pKa = 11.84 VSVTDD5028 pKa = 3.87 EE5029 pKa = 4.45 DD5030 pKa = 5.44 GVWADD5035 pKa = 3.29 AGVRR5039 pKa = 11.84 SITVNNVAPTAPVSGAATVAEE5060 pKa = 4.47 GATYY5064 pKa = 9.03 TLNVGAVTDD5073 pKa = 4.64 PGADD5077 pKa = 3.0 TRR5079 pKa = 11.84 TGYY5082 pKa = 10.47 SISWGDD5088 pKa = 3.33 GTPNEE5093 pKa = 4.64 NFTPAQWAAAGGVFTHH5109 pKa = 6.69 VYY5111 pKa = 10.35 ADD5113 pKa = 3.62 GVGAGTGRR5121 pKa = 11.84 IIVVSAADD5129 pKa = 3.53 EE5130 pKa = 4.86 DD5131 pKa = 4.35 GTHH5134 pKa = 7.34 ILGTQNLTITNTSPGLVLSGNANTLEE5160 pKa = 4.27 GAVYY5164 pKa = 9.17 TLNIVGTDD5172 pKa = 3.41 VPADD5176 pKa = 3.59 VLSYY5180 pKa = 10.91 SIDD5183 pKa = 3.46 WGDD5186 pKa = 3.5 GSALQTITAAEE5197 pKa = 3.96 LAALSGNVTHH5207 pKa = 7.14 TFADD5211 pKa = 3.47
Molecular weight: 537.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.814
IPC2_protein 3.834
IPC_protein 3.884
Toseland 3.656
ProMoST 4.037
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.706
Grimsley 3.554
Solomon 3.884
Lehninger 3.834
Nozaki 3.986
DTASelect 4.266
Thurlkill 3.694
EMBOSS 3.821
Sillero 4.012
Patrickios 2.422
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A0N1ABL1|A0A0N1ABL1_9PROT PEP-CTERM domain-containing protein OS=beta proteobacterium AAP65 OX=1523424 GN=IP80_16770 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.07 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.7 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3930
0
3930
1366771
37
5211
347.8
37.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.362 ± 0.076
0.84 ± 0.013
4.798 ± 0.029
5.15 ± 0.043
3.269 ± 0.025
8.803 ± 0.071
2.214 ± 0.026
3.455 ± 0.03
2.658 ± 0.037
11.333 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.158 ± 0.023
2.394 ± 0.032
5.673 ± 0.041
4.292 ± 0.035
7.198 ± 0.05
4.902 ± 0.044
5.232 ± 0.05
7.731 ± 0.034
1.567 ± 0.019
1.97 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here