Wuhan insect virus 34

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEF8|A0A1L3KEF8_9VIRU Uncharacterized protein OS=Wuhan insect virus 34 OX=1923737 PE=4 SV=1
MM1 pKa = 8.37DD2 pKa = 4.39ATTPDD7 pKa = 3.65NEE9 pKa = 4.5TISWILYY16 pKa = 9.17VMEE19 pKa = 4.83KK20 pKa = 10.25EE21 pKa = 4.12YY22 pKa = 11.29TEE24 pKa = 4.29AAWRR28 pKa = 11.84LPGSWWSWEE37 pKa = 3.85AFVEE41 pKa = 4.51AVRR44 pKa = 11.84HH45 pKa = 5.68LEE47 pKa = 4.11MTSSPGIPYY56 pKa = 9.43MKK58 pKa = 9.81EE59 pKa = 3.47APTIGDD65 pKa = 3.54WLRR68 pKa = 11.84WDD70 pKa = 4.86GVQCDD75 pKa = 3.92DD76 pKa = 3.84FQLRR80 pKa = 11.84RR81 pKa = 11.84LWHH84 pKa = 5.93EE85 pKa = 3.96VQQVFDD91 pKa = 3.55EE92 pKa = 4.25DD93 pKa = 3.5FRR95 pKa = 11.84LKK97 pKa = 10.83LRR99 pKa = 11.84VFIKK103 pKa = 10.37QEE105 pKa = 3.57PHH107 pKa = 6.37KK108 pKa = 10.5KK109 pKa = 10.16SKK111 pKa = 11.3AEE113 pKa = 4.04DD114 pKa = 3.7GRR116 pKa = 11.84WRR118 pKa = 11.84LILASPLNVQVAWHH132 pKa = 5.85MLFSYY137 pKa = 10.47MNDD140 pKa = 2.95IEE142 pKa = 4.32IDD144 pKa = 3.18KK145 pKa = 10.73AYY147 pKa = 10.51YY148 pKa = 10.36LPSQQGLNLSGGMWKK163 pKa = 10.48LYY165 pKa = 10.13LNQWKK170 pKa = 8.38TSRR173 pKa = 11.84QTCGMDD179 pKa = 2.93KK180 pKa = 10.22TAWDD184 pKa = 3.63WTAPWWVFLVDD195 pKa = 3.46LEE197 pKa = 4.39FRR199 pKa = 11.84RR200 pKa = 11.84RR201 pKa = 11.84MCRR204 pKa = 11.84RR205 pKa = 11.84GDD207 pKa = 3.33VGSWLRR213 pKa = 11.84KK214 pKa = 9.8AKK216 pKa = 10.3VLYY219 pKa = 10.01HH220 pKa = 6.61QMFEE224 pKa = 4.11QPILVLSDD232 pKa = 3.17GSEE235 pKa = 4.15FEE237 pKa = 3.85QLYY240 pKa = 9.34PGIMKK245 pKa = 10.08SGCVNTISTNSHH257 pKa = 5.24AQCFVHH263 pKa = 7.13LAVCRR268 pKa = 11.84KK269 pKa = 9.92NNLPYY274 pKa = 10.67YY275 pKa = 8.81PLPKK279 pKa = 10.23CVGDD283 pKa = 3.81DD284 pKa = 3.56TLSTEE289 pKa = 4.19EE290 pKa = 4.38QIPANIMDD298 pKa = 5.15LYY300 pKa = 11.34AEE302 pKa = 4.11FGVRR306 pKa = 11.84IKK308 pKa = 10.98SVTDD312 pKa = 3.0TLEE315 pKa = 3.82FVGRR319 pKa = 11.84EE320 pKa = 3.92FRR322 pKa = 11.84DD323 pKa = 3.66CGPIPMYY330 pKa = 10.36VGKK333 pKa = 9.73HH334 pKa = 3.94VAKK337 pKa = 9.93AHH339 pKa = 5.25YY340 pKa = 9.92VSEE343 pKa = 4.43EE344 pKa = 3.98NLVSYY349 pKa = 10.0LDD351 pKa = 3.57SMARR355 pKa = 11.84EE356 pKa = 4.32YY357 pKa = 10.97CHH359 pKa = 6.74SEE361 pKa = 3.4SYY363 pKa = 9.8YY364 pKa = 10.68RR365 pKa = 11.84VWEE368 pKa = 4.11ALAEE372 pKa = 4.03RR373 pKa = 11.84LGIDD377 pKa = 3.62LPLSRR382 pKa = 11.84EE383 pKa = 4.27SYY385 pKa = 10.45LYY387 pKa = 9.92WYY389 pKa = 9.51DD390 pKa = 3.62YY391 pKa = 11.12QGWW394 pKa = 3.51

Molecular weight: 46.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEF8|A0A1L3KEF8_9VIRU Uncharacterized protein OS=Wuhan insect virus 34 OX=1923737 PE=4 SV=1
MM1 pKa = 7.88VFEE4 pKa = 4.64FCSDD8 pKa = 3.33YY9 pKa = 11.39SSAIMTSLRR18 pKa = 11.84RR19 pKa = 11.84EE20 pKa = 4.07LAEE23 pKa = 4.53MIRR26 pKa = 11.84PSQPLVSLAEE36 pKa = 4.15LPPIPEE42 pKa = 4.32APLVEE47 pKa = 4.37GAVRR51 pKa = 11.84LTVEE55 pKa = 4.95AKK57 pKa = 10.24RR58 pKa = 11.84SAAGVLWSGLRR69 pKa = 11.84RR70 pKa = 11.84GVSSLMHH77 pKa = 6.75ASLDD81 pKa = 3.66GAKK84 pKa = 9.61VAKK87 pKa = 9.99EE88 pKa = 4.31CFDD91 pKa = 4.08SLPSSVKK98 pKa = 10.1VGGLIVTTAVATHH111 pKa = 7.18LIGKK115 pKa = 8.38HH116 pKa = 5.67KK117 pKa = 10.49RR118 pKa = 11.84DD119 pKa = 3.13IDD121 pKa = 3.67AKK123 pKa = 8.71VAKK126 pKa = 9.51FRR128 pKa = 11.84DD129 pKa = 3.58MFRR132 pKa = 11.84PEE134 pKa = 4.34MKK136 pKa = 10.03VVMAVGSTAKK146 pKa = 10.29ILEE149 pKa = 4.33SKK151 pKa = 10.39RR152 pKa = 11.84AGSEE156 pKa = 4.0EE157 pKa = 4.69SDD159 pKa = 2.99MTAYY163 pKa = 9.83KK164 pKa = 10.11GQCQIGCYY172 pKa = 9.68DD173 pKa = 3.25GSKK176 pKa = 10.5FVVTGAALRR185 pKa = 11.84FASNYY190 pKa = 10.14LVGPDD195 pKa = 3.65HH196 pKa = 7.32VLGASNLIAKK206 pKa = 8.42GRR208 pKa = 11.84QGRR211 pKa = 11.84VPLDD215 pKa = 3.42GKK217 pKa = 10.39EE218 pKa = 4.2RR219 pKa = 11.84IPLATDD225 pKa = 3.59LVAILLTDD233 pKa = 3.8AEE235 pKa = 4.43FANLGVSEE243 pKa = 4.23VKK245 pKa = 10.43IGVFEE250 pKa = 4.13SSIYY254 pKa = 9.81AQVVGPLGKK263 pKa = 9.35GTTGALKK270 pKa = 10.44DD271 pKa = 4.1DD272 pKa = 4.06NSCFGRR278 pKa = 11.84VIYY281 pKa = 10.42EE282 pKa = 4.02GTTLPGYY289 pKa = 10.12SGSAYY294 pKa = 10.66SSGSYY299 pKa = 10.05CYY301 pKa = 10.72GMHH304 pKa = 5.93QCGGQVNGGFSASFIWCLLRR324 pKa = 11.84IHH326 pKa = 6.96LRR328 pKa = 11.84RR329 pKa = 11.84RR330 pKa = 11.84DD331 pKa = 3.6EE332 pKa = 4.17DD333 pKa = 3.98TADD336 pKa = 2.95HH337 pKa = 6.92LMRR340 pKa = 11.84EE341 pKa = 4.27FSKK344 pKa = 11.13GKK346 pKa = 9.92GIYY349 pKa = 9.24WSTSHH354 pKa = 7.09TDD356 pKa = 3.18PSEE359 pKa = 4.11VIVRR363 pKa = 11.84INGRR367 pKa = 11.84YY368 pKa = 8.88EE369 pKa = 3.79YY370 pKa = 10.58HH371 pKa = 6.18STDD374 pKa = 2.22TMYY377 pKa = 11.24DD378 pKa = 3.2VFGNDD383 pKa = 2.88WMNKK387 pKa = 7.76TEE389 pKa = 4.26LKK391 pKa = 10.42RR392 pKa = 11.84RR393 pKa = 11.84GRR395 pKa = 11.84DD396 pKa = 3.39FQDD399 pKa = 3.63FKK401 pKa = 11.57DD402 pKa = 4.03VEE404 pKa = 4.49FEE406 pKa = 4.1SAPVSGEE413 pKa = 3.87SRR415 pKa = 11.84SLKK418 pKa = 10.63SGGSGILEE426 pKa = 4.25SPQDD430 pKa = 4.11SLPLSHH436 pKa = 6.75QSLMKK441 pKa = 10.26EE442 pKa = 4.04YY443 pKa = 10.69QKK445 pKa = 11.01LSKK448 pKa = 10.13DD449 pKa = 3.25SKK451 pKa = 9.03MKK453 pKa = 10.06FRR455 pKa = 11.84KK456 pKa = 9.65SLGMWMEE463 pKa = 4.53GPTTSGRR470 pKa = 11.84VKK472 pKa = 10.57SGNPTSS478 pKa = 3.52

Molecular weight: 52.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

872

394

478

436.0

49.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.422 ± 0.559

1.95 ± 0.223

5.734 ± 0.238

6.537 ± 0.549

3.67 ± 0.078

7.913 ± 1.722

2.294 ± 0.163

4.128 ± 0.045

5.734 ± 0.608

8.83 ± 0.374

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.44 ± 0.075

2.294 ± 0.332

4.243 ± 0.217

3.096 ± 0.643

6.078 ± 0.009

9.06 ± 1.64

4.472 ± 0.274

6.995 ± 0.264

2.867 ± 1.473

4.243 ± 0.894
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski