Moraxella phage Mcat5
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PHD5|A0A0R6PHD5_9CAUD Tail protein OS=Moraxella phage Mcat5 OX=1647551 PE=4 SV=1
MM1 pKa = 7.85 HH2 pKa = 7.07 YY3 pKa = 10.22 PVTLTPDD10 pKa = 3.17 TDD12 pKa = 4.07 GFCVTFRR19 pKa = 11.84 DD20 pKa = 3.49 IPEE23 pKa = 5.25 AISQGDD29 pKa = 3.84 TTDD32 pKa = 3.35 EE33 pKa = 4.31 ALDD36 pKa = 3.8 MAQDD40 pKa = 3.74 ALMVAMEE47 pKa = 5.96 FYY49 pKa = 11.02 FEE51 pKa = 5.02 DD52 pKa = 3.55 NRR54 pKa = 11.84 AVPMPSS60 pKa = 2.7
Molecular weight: 6.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.792
IPC2_protein 3.656
IPC_protein 3.567
Toseland 3.376
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.579
Rodwell 3.414
Grimsley 3.287
Solomon 3.554
Lehninger 3.503
Nozaki 3.732
DTASelect 3.948
Thurlkill 3.465
EMBOSS 3.579
Sillero 3.706
Patrickios 1.837
IPC_peptide 3.541
IPC2_peptide 3.668
IPC2.peptide.svr19 3.697
Protein with the highest isoelectric point:
>tr|A0A0R6PH59|A0A0R6PH59_9CAUD Uncharacterized protein OS=Moraxella phage Mcat5 OX=1647551 PE=4 SV=1
MM1 pKa = 7.7 AKK3 pKa = 10.25 LGNLPPRR10 pKa = 11.84 LKK12 pKa = 9.39 PTQSHH17 pKa = 4.64 TPKK20 pKa = 10.51 RR21 pKa = 11.84 NWGKK25 pKa = 10.32 GRR27 pKa = 11.84 EE28 pKa = 4.11 GRR30 pKa = 11.84 AWRR33 pKa = 11.84 RR34 pKa = 11.84 LRR36 pKa = 11.84 AKK38 pKa = 9.92 MLVRR42 pKa = 11.84 DD43 pKa = 4.06 NYY45 pKa = 9.72 TCQLCGRR52 pKa = 11.84 IGGRR56 pKa = 11.84 LEE58 pKa = 4.59 LDD60 pKa = 3.78 HH61 pKa = 6.99 IVNKK65 pKa = 10.57 AVGGTDD71 pKa = 4.2 DD72 pKa = 4.03 KK73 pKa = 11.72 TNLQILCYY81 pKa = 9.44 QCHH84 pKa = 4.95 KK85 pKa = 9.73 TKK87 pKa = 9.37 TQSEE91 pKa = 4.72 SKK93 pKa = 10.7 AGGVLQFLGG102 pKa = 3.78
Molecular weight: 11.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.275
IPC2_protein 9.502
IPC_protein 9.663
Toseland 10.599
ProMoST 10.116
Dawson 10.672
Bjellqvist 10.306
Wikipedia 10.804
Rodwell 11.155
Grimsley 10.701
Solomon 10.73
Lehninger 10.716
Nozaki 10.599
DTASelect 10.277
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.599
Patrickios 10.906
IPC_peptide 10.745
IPC2_peptide 9.268
IPC2.peptide.svr19 8.512
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
14626
38
1508
215.1
24.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.328 ± 0.443
0.93 ± 0.158
6.441 ± 0.201
5.504 ± 0.314
3.528 ± 0.192
6.441 ± 0.345
2.496 ± 0.234
7.029 ± 0.233
7.029 ± 0.309
8.663 ± 0.276
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.4 ± 0.234
5.128 ± 0.307
3.576 ± 0.332
4.902 ± 0.255
4.335 ± 0.155
6.352 ± 0.492
6.516 ± 0.384
5.606 ± 0.285
1.21 ± 0.088
3.589 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here