Amino acid dipepetide frequency for Mycobacterium phage Beelzebub

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.056AlaAla: 12.056 ± 1.172
1.157AlaCys: 1.157 ± 0.287
6.462AlaAsp: 6.462 ± 0.588
6.799AlaGlu: 6.799 ± 0.635
2.893AlaPhe: 2.893 ± 0.423
6.414AlaGly: 6.414 ± 0.552
2.17AlaHis: 2.17 ± 0.352
4.678AlaIle: 4.678 ± 0.413
4.485AlaLys: 4.485 ± 0.56
8.535AlaLeu: 8.535 ± 0.723
2.749AlaMet: 2.749 ± 0.349
3.617AlaAsn: 3.617 ± 0.512
4.629AlaPro: 4.629 ± 0.492
3.713AlaGln: 3.713 ± 0.565
6.221AlaArg: 6.221 ± 0.561
4.051AlaSer: 4.051 ± 0.441
5.835AlaThr: 5.835 ± 0.624
6.365AlaVal: 6.365 ± 0.739
2.074AlaTrp: 2.074 ± 0.241
2.797AlaTyr: 2.797 ± 0.358
0.0AlaXaa: 0.0 ± 0.0
Cys
1.109CysAla: 1.109 ± 0.249
0.289CysCys: 0.289 ± 0.133
1.398CysAsp: 1.398 ± 0.345
0.964CysGlu: 0.964 ± 0.269
0.241CysPhe: 0.241 ± 0.097
1.688CysGly: 1.688 ± 0.351
0.482CysHis: 0.482 ± 0.166
0.82CysIle: 0.82 ± 0.187
0.338CysLys: 0.338 ± 0.149
0.482CysLeu: 0.482 ± 0.155
0.096CysMet: 0.096 ± 0.064
0.579CysAsn: 0.579 ± 0.173
0.868CysPro: 0.868 ± 0.221
0.772CysGln: 0.772 ± 0.212
1.013CysArg: 1.013 ± 0.273
0.723CysSer: 0.723 ± 0.172
0.53CysThr: 0.53 ± 0.183
0.772CysVal: 0.772 ± 0.222
0.386CysTrp: 0.386 ± 0.16
0.434CysTyr: 0.434 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
5.015AspAla: 5.015 ± 0.453
0.772AspCys: 0.772 ± 0.206
5.063AspAsp: 5.063 ± 0.628
5.497AspGlu: 5.497 ± 0.637
2.218AspPhe: 2.218 ± 0.307
4.388AspGly: 4.388 ± 0.521
1.061AspHis: 1.061 ± 0.205
3.713AspIle: 3.713 ± 0.593
2.218AspLys: 2.218 ± 0.361
5.835AspLeu: 5.835 ± 0.559
1.832AspMet: 1.832 ± 0.318
1.977AspAsn: 1.977 ± 0.333
5.063AspPro: 5.063 ± 0.598
1.64AspGln: 1.64 ± 0.317
4.195AspArg: 4.195 ± 0.414
2.556AspSer: 2.556 ± 0.386
2.893AspThr: 2.893 ± 0.376
4.774AspVal: 4.774 ± 0.498
1.447AspTrp: 1.447 ± 0.225
2.508AspTyr: 2.508 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
7.185GluAla: 7.185 ± 0.777
0.916GluCys: 0.916 ± 0.268
3.569GluAsp: 3.569 ± 0.535
4.871GluGlu: 4.871 ± 0.588
3.279GluPhe: 3.279 ± 0.364
4.967GluGly: 4.967 ± 0.463
1.784GluHis: 1.784 ± 0.35
2.7GluIle: 2.7 ± 0.3
2.17GluLys: 2.17 ± 0.377
5.787GluLeu: 5.787 ± 0.56
2.604GluMet: 2.604 ± 0.382
2.025GluAsn: 2.025 ± 0.374
2.749GluPro: 2.749 ± 0.41
2.99GluGln: 2.99 ± 0.383
5.305GluArg: 5.305 ± 0.527
3.665GluSer: 3.665 ± 0.457
3.038GluThr: 3.038 ± 0.341
4.774GluVal: 4.774 ± 0.596
1.591GluTrp: 1.591 ± 0.279
1.736GluTyr: 1.736 ± 0.384
0.0GluXaa: 0.0 ± 0.0
Phe
2.218PheAla: 2.218 ± 0.284
0.53PheCys: 0.53 ± 0.157
2.652PheAsp: 2.652 ± 0.306
2.411PheGlu: 2.411 ± 0.314
1.013PhePhe: 1.013 ± 0.211
3.327PheGly: 3.327 ± 0.451
0.772PheHis: 0.772 ± 0.181
1.64PheIle: 1.64 ± 0.285
1.688PheLys: 1.688 ± 0.247
2.749PheLeu: 2.749 ± 0.409
0.772PheMet: 0.772 ± 0.192
1.35PheAsn: 1.35 ± 0.211
1.157PhePro: 1.157 ± 0.283
1.013PheGln: 1.013 ± 0.198
2.17PheArg: 2.17 ± 0.335
1.543PheSer: 1.543 ± 0.33
2.122PheThr: 2.122 ± 0.433
2.074PheVal: 2.074 ± 0.388
0.868PheTrp: 0.868 ± 0.222
1.157PheTyr: 1.157 ± 0.227
0.0PheXaa: 0.0 ± 0.0
Gly
6.414GlyAla: 6.414 ± 0.863
0.627GlyCys: 0.627 ± 0.179
6.028GlyAsp: 6.028 ± 0.686
4.34GlyGlu: 4.34 ± 0.435
3.713GlyPhe: 3.713 ± 0.397
8.969GlyGly: 8.969 ± 1.364
1.64GlyHis: 1.64 ± 0.308
3.424GlyIle: 3.424 ± 0.483
3.761GlyLys: 3.761 ± 0.544
6.317GlyLeu: 6.317 ± 0.595
1.977GlyMet: 1.977 ± 0.35
2.942GlyAsn: 2.942 ± 0.41
4.051GlyPro: 4.051 ± 0.704
2.845GlyGln: 2.845 ± 0.417
5.594GlyArg: 5.594 ± 0.583
5.497GlySer: 5.497 ± 0.613
4.871GlyThr: 4.871 ± 0.71
5.931GlyVal: 5.931 ± 0.622
2.266GlyTrp: 2.266 ± 0.41
2.556GlyTyr: 2.556 ± 0.388
0.0GlyXaa: 0.0 ± 0.0
His
2.122HisAla: 2.122 ± 0.385
0.289HisCys: 0.289 ± 0.111
1.398HisAsp: 1.398 ± 0.351
1.591HisGlu: 1.591 ± 0.318
0.338HisPhe: 0.338 ± 0.132
2.122HisGly: 2.122 ± 0.289
0.964HisHis: 0.964 ± 0.247
1.013HisIle: 1.013 ± 0.238
0.627HisLys: 0.627 ± 0.137
2.218HisLeu: 2.218 ± 0.334
0.338HisMet: 0.338 ± 0.135
0.82HisAsn: 0.82 ± 0.173
1.736HisPro: 1.736 ± 0.238
0.868HisGln: 0.868 ± 0.177
1.109HisArg: 1.109 ± 0.239
1.061HisSer: 1.061 ± 0.198
1.013HisThr: 1.013 ± 0.237
1.109HisVal: 1.109 ± 0.213
0.338HisTrp: 0.338 ± 0.14
0.868HisTyr: 0.868 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
4.919IleAla: 4.919 ± 0.527
0.772IleCys: 0.772 ± 0.171
4.003IleAsp: 4.003 ± 0.486
3.617IleGlu: 3.617 ± 0.387
1.206IlePhe: 1.206 ± 0.232
3.954IleGly: 3.954 ± 0.447
1.157IleHis: 1.157 ± 0.239
1.64IleIle: 1.64 ± 0.305
1.447IleLys: 1.447 ± 0.256
3.424IleLeu: 3.424 ± 0.422
0.772IleMet: 0.772 ± 0.179
1.591IleAsn: 1.591 ± 0.388
2.845IlePro: 2.845 ± 0.47
1.64IleGln: 1.64 ± 0.266
3.134IleArg: 3.134 ± 0.382
2.363IleSer: 2.363 ± 0.322
2.17IleThr: 2.17 ± 0.447
2.99IleVal: 2.99 ± 0.438
0.675IleTrp: 0.675 ± 0.192
1.206IleTyr: 1.206 ± 0.299
0.0IleXaa: 0.0 ± 0.0
Lys
4.678LysAla: 4.678 ± 0.508
0.723LysCys: 0.723 ± 0.182
1.254LysAsp: 1.254 ± 0.279
1.784LysGlu: 1.784 ± 0.375
1.398LysPhe: 1.398 ± 0.302
2.749LysGly: 2.749 ± 0.318
0.916LysHis: 0.916 ± 0.22
1.64LysIle: 1.64 ± 0.301
2.315LysLys: 2.315 ± 0.453
3.279LysLeu: 3.279 ± 0.484
1.061LysMet: 1.061 ± 0.189
1.061LysAsn: 1.061 ± 0.302
2.7LysPro: 2.7 ± 0.443
1.832LysGln: 1.832 ± 0.333
3.038LysArg: 3.038 ± 0.402
1.736LysSer: 1.736 ± 0.296
1.881LysThr: 1.881 ± 0.331
2.17LysVal: 2.17 ± 0.298
0.868LysTrp: 0.868 ± 0.163
0.53LysTyr: 0.53 ± 0.201
0.0LysXaa: 0.0 ± 0.0
Leu
8.391LeuAla: 8.391 ± 0.733
0.964LeuCys: 0.964 ± 0.273
6.124LeuAsp: 6.124 ± 0.628
5.69LeuGlu: 5.69 ± 0.524
2.652LeuPhe: 2.652 ± 0.476
6.124LeuGly: 6.124 ± 0.87
1.398LeuHis: 1.398 ± 0.297
3.954LeuIle: 3.954 ± 0.37
2.652LeuLys: 2.652 ± 0.398
6.51LeuLeu: 6.51 ± 0.55
1.832LeuMet: 1.832 ± 0.287
2.797LeuAsn: 2.797 ± 0.417
5.642LeuPro: 5.642 ± 0.623
2.7LeuGln: 2.7 ± 0.34
5.787LeuArg: 5.787 ± 0.533
4.629LeuSer: 4.629 ± 0.454
5.256LeuThr: 5.256 ± 0.475
5.546LeuVal: 5.546 ± 0.583
1.109LeuTrp: 1.109 ± 0.23
2.17LeuTyr: 2.17 ± 0.305
0.0LeuXaa: 0.0 ± 0.0
Met
3.038MetAla: 3.038 ± 0.381
0.386MetCys: 0.386 ± 0.141
0.916MetAsp: 0.916 ± 0.264
1.157MetGlu: 1.157 ± 0.237
1.254MetPhe: 1.254 ± 0.257
2.122MetGly: 2.122 ± 0.279
0.53MetHis: 0.53 ± 0.17
1.061MetIle: 1.061 ± 0.228
0.964MetLys: 0.964 ± 0.193
1.543MetLeu: 1.543 ± 0.243
0.627MetMet: 0.627 ± 0.171
1.109MetAsn: 1.109 ± 0.251
1.688MetPro: 1.688 ± 0.273
0.434MetGln: 0.434 ± 0.159
1.688MetArg: 1.688 ± 0.244
2.508MetSer: 2.508 ± 0.411
2.7MetThr: 2.7 ± 0.407
1.495MetVal: 1.495 ± 0.363
0.916MetTrp: 0.916 ± 0.195
0.193MetTyr: 0.193 ± 0.082
0.0MetXaa: 0.0 ± 0.0
Asn
4.003AsnAla: 4.003 ± 0.4
0.675AsnCys: 0.675 ± 0.199
1.881AsnAsp: 1.881 ± 0.262
1.977AsnGlu: 1.977 ± 0.301
1.157AsnPhe: 1.157 ± 0.238
3.52AsnGly: 3.52 ± 0.405
0.482AsnHis: 0.482 ± 0.138
1.591AsnIle: 1.591 ± 0.261
1.061AsnLys: 1.061 ± 0.261
2.797AsnLeu: 2.797 ± 0.356
0.482AsnMet: 0.482 ± 0.137
1.206AsnAsn: 1.206 ± 0.267
2.797AsnPro: 2.797 ± 0.426
1.64AsnGln: 1.64 ± 0.249
2.074AsnArg: 2.074 ± 0.341
1.929AsnSer: 1.929 ± 0.356
1.929AsnThr: 1.929 ± 0.357
1.977AsnVal: 1.977 ± 0.301
0.916AsnTrp: 0.916 ± 0.178
0.82AsnTyr: 0.82 ± 0.262
0.0AsnXaa: 0.0 ± 0.0
Pro
4.774ProAla: 4.774 ± 0.752
0.675ProCys: 0.675 ± 0.174
3.472ProAsp: 3.472 ± 0.477
4.678ProGlu: 4.678 ± 0.681
1.881ProPhe: 1.881 ± 0.281
5.642ProGly: 5.642 ± 0.721
1.591ProHis: 1.591 ± 0.306
2.411ProIle: 2.411 ± 0.373
2.074ProLys: 2.074 ± 0.323
3.858ProLeu: 3.858 ± 0.393
1.398ProMet: 1.398 ± 0.237
2.266ProAsn: 2.266 ± 0.383
3.327ProPro: 3.327 ± 0.498
2.17ProGln: 2.17 ± 0.324
3.183ProArg: 3.183 ± 0.421
4.822ProSer: 4.822 ± 0.529
3.376ProThr: 3.376 ± 0.523
3.472ProVal: 3.472 ± 0.405
1.061ProTrp: 1.061 ± 0.228
1.447ProTyr: 1.447 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
3.665GlnAla: 3.665 ± 0.576
0.723GlnCys: 0.723 ± 0.2
1.64GlnAsp: 1.64 ± 0.305
2.266GlnGlu: 2.266 ± 0.278
0.868GlnPhe: 0.868 ± 0.179
2.459GlnGly: 2.459 ± 0.381
0.772GlnHis: 0.772 ± 0.182
1.929GlnIle: 1.929 ± 0.312
1.591GlnLys: 1.591 ± 0.278
3.81GlnLeu: 3.81 ± 0.531
1.254GlnMet: 1.254 ± 0.212
0.772GlnAsn: 0.772 ± 0.132
1.929GlnPro: 1.929 ± 0.249
2.025GlnGln: 2.025 ± 0.419
3.183GlnArg: 3.183 ± 0.463
1.977GlnSer: 1.977 ± 0.337
1.688GlnThr: 1.688 ± 0.249
2.652GlnVal: 2.652 ± 0.367
1.061GlnTrp: 1.061 ± 0.227
0.723GlnTyr: 0.723 ± 0.164
0.0GlnXaa: 0.0 ± 0.0
Arg
6.221ArgAla: 6.221 ± 0.617
1.109ArgCys: 1.109 ± 0.288
4.147ArgAsp: 4.147 ± 0.494
4.678ArgGlu: 4.678 ± 0.478
2.315ArgPhe: 2.315 ± 0.399
5.16ArgGly: 5.16 ± 0.566
1.398ArgHis: 1.398 ± 0.331
3.231ArgIle: 3.231 ± 0.329
3.183ArgLys: 3.183 ± 0.462
6.124ArgLeu: 6.124 ± 0.642
1.591ArgMet: 1.591 ± 0.278
2.266ArgAsn: 2.266 ± 0.308
3.279ArgPro: 3.279 ± 0.367
2.556ArgGln: 2.556 ± 0.343
6.269ArgArg: 6.269 ± 0.813
2.99ArgSer: 2.99 ± 0.331
2.7ArgThr: 2.7 ± 0.369
4.099ArgVal: 4.099 ± 0.479
2.315ArgTrp: 2.315 ± 0.437
2.025ArgTyr: 2.025 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
5.497SerAla: 5.497 ± 0.625
0.482SerCys: 0.482 ± 0.176
4.195SerAsp: 4.195 ± 0.533
3.231SerGlu: 3.231 ± 0.464
1.736SerPhe: 1.736 ± 0.37
5.69SerGly: 5.69 ± 0.898
1.398SerHis: 1.398 ± 0.33
2.508SerIle: 2.508 ± 0.37
1.495SerLys: 1.495 ± 0.22
4.388SerLeu: 4.388 ± 0.556
1.881SerMet: 1.881 ± 0.316
1.784SerAsn: 1.784 ± 0.361
3.038SerPro: 3.038 ± 0.355
1.881SerGln: 1.881 ± 0.298
3.713SerArg: 3.713 ± 0.588
3.713SerSer: 3.713 ± 0.501
3.231SerThr: 3.231 ± 0.327
3.424SerVal: 3.424 ± 0.401
1.302SerTrp: 1.302 ± 0.254
1.013SerTyr: 1.013 ± 0.187
0.0SerXaa: 0.0 ± 0.0
Thr
5.497ThrAla: 5.497 ± 0.536
0.772ThrCys: 0.772 ± 0.223
3.424ThrAsp: 3.424 ± 0.362
2.749ThrGlu: 2.749 ± 0.406
1.736ThrPhe: 1.736 ± 0.279
5.16ThrGly: 5.16 ± 0.574
1.157ThrHis: 1.157 ± 0.251
2.652ThrIle: 2.652 ± 0.343
1.736ThrLys: 1.736 ± 0.264
5.16ThrLeu: 5.16 ± 0.691
1.495ThrMet: 1.495 ± 0.326
2.266ThrAsn: 2.266 ± 0.406
3.858ThrPro: 3.858 ± 0.576
2.218ThrGln: 2.218 ± 0.282
3.424ThrArg: 3.424 ± 0.438
2.508ThrSer: 2.508 ± 0.42
3.183ThrThr: 3.183 ± 0.465
4.147ThrVal: 4.147 ± 0.593
1.254ThrTrp: 1.254 ± 0.283
1.206ThrTyr: 1.206 ± 0.222
0.0ThrXaa: 0.0 ± 0.0
Val
6.992ValAla: 6.992 ± 0.688
1.206ValCys: 1.206 ± 0.294
3.472ValAsp: 3.472 ± 0.389
5.546ValGlu: 5.546 ± 0.459
1.977ValPhe: 1.977 ± 0.294
5.015ValGly: 5.015 ± 0.583
0.868ValHis: 0.868 ± 0.206
2.99ValIle: 2.99 ± 0.474
2.459ValLys: 2.459 ± 0.344
5.305ValLeu: 5.305 ± 0.474
1.977ValMet: 1.977 ± 0.339
2.797ValAsn: 2.797 ± 0.371
4.244ValPro: 4.244 ± 0.506
2.17ValGln: 2.17 ± 0.424
3.906ValArg: 3.906 ± 0.429
4.437ValSer: 4.437 ± 0.419
4.003ValThr: 4.003 ± 0.594
5.931ValVal: 5.931 ± 0.633
0.868ValTrp: 0.868 ± 0.254
1.543ValTyr: 1.543 ± 0.266
0.0ValXaa: 0.0 ± 0.0
Trp
1.447TrpAla: 1.447 ± 0.276
0.627TrpCys: 0.627 ± 0.174
1.688TrpAsp: 1.688 ± 0.263
1.688TrpGlu: 1.688 ± 0.307
0.579TrpPhe: 0.579 ± 0.155
1.832TrpGly: 1.832 ± 0.327
0.868TrpHis: 0.868 ± 0.243
0.675TrpIle: 0.675 ± 0.206
0.82TrpLys: 0.82 ± 0.193
1.881TrpLeu: 1.881 ± 0.332
0.772TrpMet: 0.772 ± 0.183
0.675TrpAsn: 0.675 ± 0.133
0.82TrpPro: 0.82 ± 0.197
0.964TrpGln: 0.964 ± 0.179
1.398TrpArg: 1.398 ± 0.26
1.447TrpSer: 1.447 ± 0.342
1.398TrpThr: 1.398 ± 0.274
1.977TrpVal: 1.977 ± 0.328
0.772TrpTrp: 0.772 ± 0.192
0.675TrpTyr: 0.675 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.459TyrAla: 2.459 ± 0.379
0.289TyrCys: 0.289 ± 0.135
1.881TyrAsp: 1.881 ± 0.273
2.17TyrGlu: 2.17 ± 0.331
0.772TyrPhe: 0.772 ± 0.179
2.508TyrGly: 2.508 ± 0.339
0.53TyrHis: 0.53 ± 0.177
1.254TyrIle: 1.254 ± 0.291
0.53TyrLys: 0.53 ± 0.193
1.929TyrLeu: 1.929 ± 0.371
0.772TyrMet: 0.772 ± 0.176
1.013TyrAsn: 1.013 ± 0.175
1.157TyrPro: 1.157 ± 0.263
0.916TyrGln: 0.916 ± 0.193
1.254TyrArg: 1.254 ± 0.225
1.447TyrSer: 1.447 ± 0.231
1.784TyrThr: 1.784 ± 0.324
2.025TyrVal: 2.025 ± 0.266
0.916TyrTrp: 0.916 ± 0.203
0.964TyrTyr: 0.964 ± 0.199
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 118 proteins (20738 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski