Gordonia phage Sixama

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F3Z0|A0A5Q2F3Z0_9CAUD Uncharacterized protein OS=Gordonia phage Sixama OX=2653271 GN=28 PE=4 SV=1
MM1 pKa = 8.37DD2 pKa = 6.01DD3 pKa = 3.64PALAQLRR10 pKa = 11.84AQYY13 pKa = 10.7PEE15 pKa = 4.18FTEE18 pKa = 5.48LIYY21 pKa = 11.02GPEE24 pKa = 4.05DD25 pKa = 3.39SEE27 pKa = 4.86LRR29 pKa = 11.84TRR31 pKa = 11.84DD32 pKa = 3.08GDD34 pKa = 3.54RR35 pKa = 11.84LVYY38 pKa = 9.73TEE40 pKa = 4.87PSNDD44 pKa = 3.21GNLEE48 pKa = 4.09GGWCVFPEE56 pKa = 4.48GEE58 pKa = 4.56EE59 pKa = 4.24YY60 pKa = 7.81PTLCEE65 pKa = 3.85YY66 pKa = 10.89FEE68 pKa = 4.69PRR70 pKa = 11.84SEE72 pKa = 4.14EE73 pKa = 3.86

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F3Y0|A0A5Q2F3Y0_9CAUD Uncharacterized protein OS=Gordonia phage Sixama OX=2653271 GN=18 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.03IVNRR6 pKa = 11.84VPTLKK11 pKa = 10.47LRR13 pKa = 11.84ARR15 pKa = 11.84CPFTRR20 pKa = 11.84QFHH23 pKa = 6.3SGAVMRR29 pKa = 11.84CTLIAGHH36 pKa = 7.46DD37 pKa = 3.94DD38 pKa = 3.7GAEE41 pKa = 3.94VRR43 pKa = 11.84PNGYY47 pKa = 8.49PHH49 pKa = 7.43FFTPLAAFDD58 pKa = 3.43RR59 pKa = 11.84HH60 pKa = 6.93GEE62 pKa = 3.58IRR64 pKa = 11.84YY65 pKa = 9.8NGITRR70 pKa = 11.84TIPHH74 pKa = 6.86NIFRR78 pKa = 11.84GYY80 pKa = 10.45VDD82 pKa = 3.03PHH84 pKa = 6.87EE85 pKa = 4.2YY86 pKa = 8.98TRR88 pKa = 11.84AYY90 pKa = 10.6VEE92 pKa = 3.98AHH94 pKa = 5.88SKK96 pKa = 10.46RR97 pKa = 11.84LRR99 pKa = 11.84TWDD102 pKa = 3.09RR103 pKa = 11.84TGRR106 pKa = 3.61

Molecular weight: 12.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

168

0

168

35001

38

1951

208.3

23.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.045 ± 0.277

1.026 ± 0.114

6.511 ± 0.191

6.348 ± 0.21

3.051 ± 0.104

7.014 ± 0.291

2.234 ± 0.137

5.486 ± 0.149

5.588 ± 0.266

7.837 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.101

4.057 ± 0.154

5.034 ± 0.148

3.348 ± 0.11

6.143 ± 0.278

6.354 ± 0.218

6.423 ± 0.205

6.808 ± 0.139

2.031 ± 0.085

3.18 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski