Mycoplasma virus P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Delislevirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9FZR3|Q9FZR3_9CAUD p23 OS=Mycoplasma virus P1 OX=35238 GN=orf6 PE=4 SV=1
MM1 pKa = 7.8LYY3 pKa = 10.75YY4 pKa = 10.2IFNSDD9 pKa = 2.88VNYY12 pKa = 10.46FDD14 pKa = 3.96NPKK17 pKa = 10.75NKK19 pKa = 8.53TLRR22 pKa = 11.84QWILRR27 pKa = 11.84HH28 pKa = 5.89FNTSLQGSFDD38 pKa = 4.13LSWAQEE44 pKa = 4.04RR45 pKa = 11.84FFSFQSDD52 pKa = 3.83QEE54 pKa = 4.4VKK56 pKa = 10.5RR57 pKa = 11.84LFLISPTNDD66 pKa = 2.99KK67 pKa = 10.91NDD69 pKa = 3.76PKK71 pKa = 11.33AQICYY76 pKa = 9.93VNNIEE81 pKa = 5.42YY82 pKa = 10.65LDD84 pKa = 3.54SDD86 pKa = 4.11KK87 pKa = 11.28NLKK90 pKa = 10.12RR91 pKa = 11.84IYY93 pKa = 10.96AEE95 pKa = 3.46VDD97 pKa = 2.98SFYY100 pKa = 10.81DD101 pKa = 4.25INIDD105 pKa = 4.94DD106 pKa = 4.39IGGKK110 pKa = 9.46LKK112 pKa = 9.81RR113 pKa = 11.84TNNWLLFEE121 pKa = 4.62GKK123 pKa = 7.66YY124 pKa = 10.38HH125 pKa = 7.02KK126 pKa = 10.63NLLALEE132 pKa = 5.11GINLPKK138 pKa = 10.28LDD140 pKa = 3.81FEE142 pKa = 4.88KK143 pKa = 11.05VVTLDD148 pKa = 3.18YY149 pKa = 11.11YY150 pKa = 9.87KK151 pKa = 10.79QKK153 pKa = 10.79DD154 pKa = 3.19ISFQIRR160 pKa = 11.84DD161 pKa = 3.23KK162 pKa = 11.07AIRR165 pKa = 11.84LEE167 pKa = 3.87RR168 pKa = 11.84DD169 pKa = 3.31YY170 pKa = 11.4LIKK173 pKa = 10.89DD174 pKa = 3.26NNFIRR179 pKa = 11.84KK180 pKa = 8.16PLQYY184 pKa = 11.02SLDD187 pKa = 3.58FLEE190 pKa = 5.97RR191 pKa = 11.84IEE193 pKa = 5.03DD194 pKa = 3.7LQTRR198 pKa = 11.84EE199 pKa = 3.77MLEE202 pKa = 3.91AQEE205 pKa = 4.14EE206 pKa = 4.6NFWFWQNNQRR216 pKa = 11.84VPDD219 pKa = 4.09SIINHH224 pKa = 6.15FKK226 pKa = 10.87NNQSFQRR233 pKa = 11.84YY234 pKa = 7.9SVSDD238 pKa = 3.35QEE240 pKa = 4.67IINFINRR247 pKa = 11.84WYY249 pKa = 10.3DD250 pKa = 3.13RR251 pKa = 11.84WIFWLPTSTSTFIVISEE268 pKa = 4.29KK269 pKa = 10.71KK270 pKa = 9.25VVAINFLFTKK280 pKa = 9.7RR281 pKa = 11.84TYY283 pKa = 11.22NEE285 pKa = 4.1GNPPNHH291 pKa = 6.63RR292 pKa = 11.84LVEE295 pKa = 4.31RR296 pKa = 11.84NTIDD300 pKa = 3.65YY301 pKa = 10.17SEE303 pKa = 4.17QSIASEE309 pKa = 4.16EE310 pKa = 4.07WSDD313 pKa = 4.26LIFWKK318 pKa = 10.71SLAIVGLPEE327 pKa = 3.64IKK329 pKa = 10.41GAFVSWLKK337 pKa = 10.86EE338 pKa = 3.83KK339 pKa = 10.83AHH341 pKa = 6.67LNLEE345 pKa = 4.43PTFNTYY351 pKa = 11.2NDD353 pKa = 2.91VKK355 pKa = 10.41TFIEE359 pKa = 4.48EE360 pKa = 4.23KK361 pKa = 10.46FKK363 pKa = 11.66SNANGFLYY371 pKa = 10.44KK372 pKa = 9.3PTQEE376 pKa = 5.54LIIDD380 pKa = 4.31FNNLEE385 pKa = 4.03PLMNKK390 pKa = 8.31EE391 pKa = 3.57WTTLTFSKK399 pKa = 9.95KK400 pKa = 10.03HH401 pKa = 5.95KK402 pKa = 9.67IALSKK407 pKa = 10.9EE408 pKa = 3.75FVEE411 pKa = 4.48YY412 pKa = 10.58QVISPNCTMIIDD424 pKa = 4.02YY425 pKa = 9.07EE426 pKa = 4.27QVVAKK431 pKa = 10.61EE432 pKa = 4.17NFNDD436 pKa = 4.13SIRR439 pKa = 11.84FRR441 pKa = 11.84YY442 pKa = 9.74SEE444 pKa = 4.56IEE446 pKa = 4.05TQEE449 pKa = 3.55AGYY452 pKa = 10.34CILEE456 pKa = 4.18VEE458 pKa = 4.52GQDD461 pKa = 3.5FRR463 pKa = 11.84QVQSYY468 pKa = 10.02LKK470 pKa = 10.56NDD472 pKa = 3.51VLITNAYY479 pKa = 9.29KK480 pKa = 10.62SHH482 pKa = 7.43LDD484 pKa = 3.77ANWLSHH490 pKa = 5.41QFSSQSLQIAKK501 pKa = 9.51QRR503 pKa = 11.84ADD505 pKa = 3.55NDD507 pKa = 3.62FLLNTIKK514 pKa = 10.81NAIALPFSAISSFGSTFQGGSVVAGLNAIGGVINTGFNFASSIIGGLRR562 pKa = 11.84AQEE565 pKa = 3.96DD566 pKa = 3.79AQRR569 pKa = 11.84AIEE572 pKa = 4.64GFNATYY578 pKa = 10.83KK579 pKa = 10.57DD580 pKa = 3.74LQSQEE585 pKa = 4.54RR586 pKa = 11.84FLSNNTSLTSLLINKK601 pKa = 8.81EE602 pKa = 3.96NEE604 pKa = 3.41IALYY608 pKa = 9.4IAKK611 pKa = 9.55PVEE614 pKa = 3.97KK615 pKa = 10.33DD616 pKa = 3.14LYY618 pKa = 10.67ALNRR622 pKa = 11.84YY623 pKa = 7.91FQDD626 pKa = 3.68FGQSVNDD633 pKa = 3.67YY634 pKa = 10.72YY635 pKa = 11.51SYY637 pKa = 11.62SLYY640 pKa = 10.87NINEE644 pKa = 3.93ALKK647 pKa = 9.6FSIYY651 pKa = 10.9GLYY654 pKa = 10.31NQFEE658 pKa = 4.82YY659 pKa = 10.61IEE661 pKa = 5.52DD662 pKa = 3.63IPNVSWEE669 pKa = 4.01VNNYY673 pKa = 9.76IKK675 pKa = 10.85DD676 pKa = 3.61LAKK679 pKa = 9.8TGFWIKK685 pKa = 10.63KK686 pKa = 8.76YY687 pKa = 10.54DD688 pKa = 3.33

Molecular weight: 80.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9FZR8|Q9FZR8_9CAUD DNA-directed DNA polymerase OS=Mycoplasma virus P1 OX=35238 GN=orf1 PE=3 SV=1
MM1 pKa = 7.69SRR3 pKa = 11.84RR4 pKa = 11.84DD5 pKa = 3.75KK6 pKa = 11.56LKK8 pKa = 9.91DD9 pKa = 3.06TYY11 pKa = 11.73KK12 pKa = 10.79LFKK15 pKa = 10.66DD16 pKa = 3.5DD17 pKa = 5.6FKK19 pKa = 11.83ALGYY23 pKa = 9.54SQNKK27 pKa = 7.51FVKK30 pKa = 9.08EE31 pKa = 4.1FAHH34 pKa = 5.73VRR36 pKa = 11.84YY37 pKa = 9.54YY38 pKa = 11.02DD39 pKa = 3.54KK40 pKa = 10.97KK41 pKa = 10.4KK42 pKa = 10.89KK43 pKa = 8.3EE44 pKa = 4.0LKK46 pKa = 9.68ISKK49 pKa = 9.89INNRR53 pKa = 11.84LIAKK57 pKa = 9.73RR58 pKa = 11.84EE59 pKa = 3.67RR60 pKa = 11.84YY61 pKa = 8.0YY62 pKa = 11.19QKK64 pKa = 10.23LTTTSKK70 pKa = 10.81KK71 pKa = 10.35QINEE75 pKa = 3.83NLTKK79 pKa = 10.22EE80 pKa = 4.49FSNIAHH86 pKa = 6.99FSLKK90 pKa = 10.33ARR92 pKa = 11.84NLKK95 pKa = 7.99EE96 pKa = 4.16TQLKK100 pKa = 10.92SNIKK104 pKa = 8.5NTLNTLEE111 pKa = 4.21KK112 pKa = 10.51YY113 pKa = 10.15IRR115 pKa = 11.84TEE117 pKa = 3.77EE118 pKa = 3.82DD119 pKa = 2.53RR120 pKa = 11.84KK121 pKa = 10.71RR122 pKa = 11.84LFNIKK127 pKa = 10.03FVVNHH132 pKa = 6.99KK133 pKa = 9.21DD134 pKa = 3.39QKK136 pKa = 10.67VMNGLAFVNYY146 pKa = 7.84LTNHH150 pKa = 6.36GKK152 pKa = 10.18LDD154 pKa = 4.0SYY156 pKa = 10.57PFNYY160 pKa = 10.19SNEE163 pKa = 4.23QIKK166 pKa = 10.62SATKK170 pKa = 9.74ALRR173 pKa = 11.84YY174 pKa = 9.08VLKK177 pKa = 11.01NKK179 pKa = 9.74IDD181 pKa = 3.9FNSTKK186 pKa = 10.94DD187 pKa = 3.21FTKK190 pKa = 10.92YY191 pKa = 10.77FNTSLNKK198 pKa = 10.0QEE200 pKa = 4.15IKK202 pKa = 10.57KK203 pKa = 10.06HH204 pKa = 4.24GFKK207 pKa = 10.22TFSASEE213 pKa = 4.45VYY215 pKa = 10.38GVAGSTFNNLGFLRR229 pKa = 11.84VEE231 pKa = 3.98LTTYY235 pKa = 10.69DD236 pKa = 4.5SKK238 pKa = 11.34FQSQRR243 pKa = 11.84VNNVEE248 pKa = 3.98LVGEE252 pKa = 4.22SSKK255 pKa = 11.58ALIKK259 pKa = 9.27WAAYY263 pKa = 9.53LKK265 pKa = 10.57QKK267 pKa = 10.57GINQFLIDD275 pKa = 4.22AFGVDD280 pKa = 3.31IAEE283 pKa = 4.14NMHH286 pKa = 6.99LYY288 pKa = 10.27GPKK291 pKa = 8.71NSKK294 pKa = 8.86WLFVNEE300 pKa = 4.12KK301 pKa = 8.76DD302 pKa = 3.18HH303 pKa = 7.27EE304 pKa = 4.53YY305 pKa = 11.02FFFYY309 pKa = 10.55FNSAKK314 pKa = 10.15EE315 pKa = 3.63IYY317 pKa = 9.14YY318 pKa = 10.83ANRR321 pKa = 3.4

Molecular weight: 38.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

3579

80

694

325.4

38.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.744 ± 0.544

0.363 ± 0.154

5.979 ± 0.376

7.376 ± 0.509

6.706 ± 0.277

3.325 ± 0.391

1.565 ± 0.224

8.243 ± 0.502

10.198 ± 0.965

9.36 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.76 ± 0.335

9.193 ± 0.898

2.012 ± 0.269

4.219 ± 0.349

3.744 ± 0.26

6.454 ± 0.474

4.722 ± 0.302

4.331 ± 0.234

1.146 ± 0.306

5.56 ± 0.458
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski