Zhouia amylolytica AD3

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Zhouia; Zhouia amylolytica

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W2UL28|W2UL28_9FLAO Sulfate transporter OS=Zhouia amylolytica AD3 OX=1286632 GN=P278_26320 PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.22INYY5 pKa = 8.36KK6 pKa = 9.57WLLLLAFGFIACDD19 pKa = 3.46SDD21 pKa = 5.81DD22 pKa = 4.48DD23 pKa = 4.16MSSEE27 pKa = 4.21GPDD30 pKa = 3.05AVMLTSGEE38 pKa = 4.36ADD40 pKa = 3.44FSTYY44 pKa = 10.39VAVGSSLSAGYY55 pKa = 10.43SDD57 pKa = 3.8GALFIASQINSFPNILASKK76 pKa = 9.51FQMAGGGAFTQPLMGDD92 pKa = 3.46NLGGMLVGGNQLLEE106 pKa = 3.8NRR108 pKa = 11.84LFFNGEE114 pKa = 3.83LPEE117 pKa = 4.51RR118 pKa = 11.84LPGTPTTEE126 pKa = 4.16ATNVMPGPYY135 pKa = 9.92NNMGITGAKK144 pKa = 9.11IFHH147 pKa = 6.87LGVEE151 pKa = 5.48GYY153 pKa = 10.29GSSAALSVGAANPYY167 pKa = 7.2YY168 pKa = 11.07VRR170 pKa = 11.84MASSPGASVIGDD182 pKa = 4.2ALSLAPTFFTLWAGSDD198 pKa = 3.72DD199 pKa = 3.81VLSYY203 pKa = 10.91AVSGGIGEE211 pKa = 4.32DD212 pKa = 3.23QAGNFNPEE220 pKa = 4.12TYY222 pKa = 10.39GSNDD226 pKa = 2.75ITDD229 pKa = 3.92PAVFSQAFSGILTLLMSNGAKK250 pKa = 9.93GVVANIPDD258 pKa = 3.96VTALPYY264 pKa = 8.98FTTVPHH270 pKa = 6.92APLDD274 pKa = 3.73PTNEE278 pKa = 4.05DD279 pKa = 3.81FGPQIPLLNTIFGALNQIFVALGEE303 pKa = 4.32GEE305 pKa = 4.12RR306 pKa = 11.84AVVFSEE312 pKa = 4.73TSASPVVIKK321 pKa = 10.75DD322 pKa = 3.4EE323 pKa = 4.26SLTDD327 pKa = 3.36ISAQIEE333 pKa = 4.09AALNASEE340 pKa = 4.79TFPQFIAQFGLPPEE354 pKa = 4.51AAPGVANLLGLMYY367 pKa = 10.32GQSRR371 pKa = 11.84QATEE375 pKa = 3.78ADD377 pKa = 3.94LLVLTSSSVIGEE389 pKa = 4.25VNTAAVQFLISRR401 pKa = 11.84GLSQEE406 pKa = 3.84LAAQFSVEE414 pKa = 4.34GITYY418 pKa = 9.61PLDD421 pKa = 4.52DD422 pKa = 4.28GWVLLPSEE430 pKa = 4.1QNEE433 pKa = 4.2IKK435 pKa = 9.83TATTAFNTTIEE446 pKa = 4.36SLASQNGLAFVDD458 pKa = 4.04AHH460 pKa = 7.87ALMNEE465 pKa = 4.4VASGGLPFDD474 pKa = 3.94EE475 pKa = 4.84FTLNSSLVFGNTFSLDD491 pKa = 3.46GVHH494 pKa = 6.08PTARR498 pKa = 11.84GYY500 pKa = 11.57AFIANQFMLAIDD512 pKa = 3.86AAYY515 pKa = 10.16GSNFEE520 pKa = 4.25EE521 pKa = 4.89AGVLAKK527 pKa = 10.84AGDD530 pKa = 4.25YY531 pKa = 9.48GTMYY535 pKa = 10.4PVEE538 pKa = 4.31LPP540 pKa = 3.48

Molecular weight: 56.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W2UPN9|W2UPN9_9FLAO Uncharacterized protein OS=Zhouia amylolytica AD3 OX=1286632 GN=P278_16900 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.13RR10 pKa = 11.84KK11 pKa = 9.48RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.49HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.19RR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 9.14VLAKK33 pKa = 10.18RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.07GRR39 pKa = 11.84KK40 pKa = 7.95KK41 pKa = 10.52LSVSSEE47 pKa = 3.87PRR49 pKa = 11.84HH50 pKa = 5.92KK51 pKa = 10.61KK52 pKa = 9.84

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3353

0

3353

1128980

37

4606

336.7

38.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.365 ± 0.041

0.708 ± 0.014

5.739 ± 0.037

6.859 ± 0.043

5.015 ± 0.034

6.554 ± 0.046

1.901 ± 0.023

7.699 ± 0.041

7.649 ± 0.057

9.204 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.017

6.006 ± 0.032

3.511 ± 0.023

3.329 ± 0.024

3.595 ± 0.027

6.347 ± 0.033

5.496 ± 0.049

6.313 ± 0.034

1.15 ± 0.018

4.275 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski