Klebsiella phage KpV71
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142F0X9|A0A142F0X9_9CAUD Uncharacterized protein OS=Klebsiella phage KpV71 OX=1796998 GN=kpv71_07 PE=4 SV=1
MM1 pKa = 7.26 VNVFNIIVTSAMLVLGNDD19 pKa = 3.45 VNNPVPYY26 pKa = 9.36 CTVQLQQPATQEE38 pKa = 3.85 PRR40 pKa = 11.84 PRR42 pKa = 11.84 PAYY45 pKa = 10.17 DD46 pKa = 3.39 LFEE49 pKa = 6.22 DD50 pKa = 4.39 PEE52 pKa = 4.87 GGCKK56 pKa = 9.76 EE57 pKa = 3.82 LGARR61 pKa = 11.84 ILAAVQEE68 pKa = 4.52 QYY70 pKa = 11.29 PDD72 pKa = 3.32 AAVTLTVDD80 pKa = 3.9 GKK82 pKa = 11.4 SNNDD86 pKa = 2.72 II87 pKa = 4.2
Molecular weight: 9.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.131
IPC2_protein 4.177
IPC_protein 4.037
Toseland 3.846
ProMoST 4.202
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.948
Rodwell 3.872
Grimsley 3.77
Solomon 3.999
Lehninger 3.961
Nozaki 4.151
DTASelect 4.329
Thurlkill 3.91
EMBOSS 3.961
Sillero 4.151
Patrickios 3.16
IPC_peptide 3.999
IPC2_peptide 4.139
IPC2.peptide.svr19 4.063
Protein with the highest isoelectric point:
>tr|A0A142F0Z8|A0A142F0Z8_9CAUD Uncharacterized protein OS=Klebsiella phage KpV71 OX=1796998 GN=kpv71_26 PE=4 SV=1
MM1 pKa = 7.82 NIITWVKK8 pKa = 8.2 EE9 pKa = 3.78 QYY11 pKa = 11.13 AVFLLLRR18 pKa = 11.84 AQRR21 pKa = 11.84 LQKK24 pKa = 10.75 RR25 pKa = 11.84 ADD27 pKa = 3.42 DD28 pKa = 3.92 WRR30 pKa = 11.84 WAANSHH36 pKa = 5.34 AHH38 pKa = 6.28 RR39 pKa = 11.84 ASLLGPEE46 pKa = 4.75 ISAQRR51 pKa = 11.84 YY52 pKa = 7.72 HH53 pKa = 7.76 LNRR56 pKa = 11.84 QCAKK60 pKa = 9.38 SRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 AYY66 pKa = 10.62 ALGAEE71 pKa = 4.45 ATATEE76 pKa = 4.55 TKK78 pKa = 10.23 AHH80 pKa = 5.85 NFISKK85 pKa = 10.22 HH86 pKa = 4.86 KK87 pKa = 10.71 LKK89 pKa = 11.07 GFDD92 pKa = 3.17
Molecular weight: 10.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.765
IPC_protein 10.467
Toseland 10.76
ProMoST 10.394
Dawson 10.847
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.155
Grimsley 10.891
Solomon 10.95
Lehninger 10.921
Nozaki 10.73
DTASelect 10.526
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.774
Patrickios 10.906
IPC_peptide 10.95
IPC2_peptide 9.385
IPC2.peptide.svr19 8.6
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
13551
51
1232
255.7
28.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.538 ± 0.549
1.225 ± 0.2
6.147 ± 0.239
5.881 ± 0.427
3.129 ± 0.182
7.276 ± 0.254
1.852 ± 0.187
4.324 ± 0.185
4.922 ± 0.322
8.671 ± 0.232
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.9 ± 0.175
3.941 ± 0.222
3.793 ± 0.262
4.575 ± 0.364
6.118 ± 0.299
6.265 ± 0.386
5.808 ± 0.283
7.232 ± 0.259
1.498 ± 0.12
3.904 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here