Spinach severe curly top virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Curtovirus

Average proteome isoelectric point is 7.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0YA56|E0YA56_9GEMI Capsid protein OS=Spinach severe curly top virus OX=873160 GN=V1 PE=3 SV=1
MM1 pKa = 7.57GPFKK5 pKa = 10.86VDD7 pKa = 3.26QFPGNYY13 pKa = 8.72PAFLAVSTSCFLRR26 pKa = 11.84YY27 pKa = 9.84NKK29 pKa = 9.25WCILGVIPEE38 pKa = 4.18IGEE41 pKa = 4.02LTLEE45 pKa = 4.18EE46 pKa = 4.94GEE48 pKa = 5.25VFLQFQKK55 pKa = 10.65EE56 pKa = 4.5VKK58 pKa = 10.11KK59 pKa = 10.69LLRR62 pKa = 11.84LNCSFGRR69 pKa = 11.84KK70 pKa = 9.12CILYY74 pKa = 9.04QDD76 pKa = 3.83IYY78 pKa = 10.76KK79 pKa = 10.41KK80 pKa = 9.99YY81 pKa = 11.02VKK83 pKa = 10.54DD84 pKa = 3.54EE85 pKa = 4.33SEE87 pKa = 4.27KK88 pKa = 10.09KK89 pKa = 10.4NAVAGCWEE97 pKa = 4.19EE98 pKa = 4.24EE99 pKa = 4.27EE100 pKa = 4.73EE101 pKa = 4.37DD102 pKa = 4.51SIWEE106 pKa = 4.42EE107 pKa = 3.74IPMEE111 pKa = 4.18EE112 pKa = 4.56VCSKK116 pKa = 8.48EE117 pKa = 5.07QKK119 pKa = 10.54DD120 pKa = 3.59QTQDD124 pKa = 2.88VV125 pKa = 3.81

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0YA57|E0YA57_9GEMI C2 protein OS=Spinach severe curly top virus OX=873160 GN=C2 PE=4 SV=2
MM1 pKa = 6.28SQKK4 pKa = 10.8RR5 pKa = 11.84KK6 pKa = 8.27MQSQAAGKK14 pKa = 9.31KK15 pKa = 9.28KK16 pKa = 10.42KK17 pKa = 9.04KK18 pKa = 8.07TVYY21 pKa = 9.65GKK23 pKa = 10.0KK24 pKa = 8.82YY25 pKa = 6.93QWKK28 pKa = 9.48RR29 pKa = 11.84SVRR32 pKa = 11.84KK33 pKa = 9.18NRR35 pKa = 11.84KK36 pKa = 8.71IKK38 pKa = 10.82LKK40 pKa = 10.14MYY42 pKa = 10.75DD43 pKa = 3.8DD44 pKa = 3.94MLGAGGVGSAITNDD58 pKa = 3.77GMITMLNNYY67 pKa = 7.83VQGIGDD73 pKa = 3.95SQRR76 pKa = 11.84SSNVTVTRR84 pKa = 11.84HH85 pKa = 5.6LKK87 pKa = 10.41YY88 pKa = 11.21DD89 pKa = 3.5MALMGSTGFWEE100 pKa = 4.79APNYY104 pKa = 5.17MTQYY108 pKa = 9.98HH109 pKa = 7.01WIIVDD114 pKa = 3.52RR115 pKa = 11.84DD116 pKa = 3.7VGSVFPDD123 pKa = 3.34KK124 pKa = 11.1LSMIFDD130 pKa = 3.84IPSNGQAMPSTYY142 pKa = 10.14RR143 pKa = 11.84IRR145 pKa = 11.84RR146 pKa = 11.84DD147 pKa = 3.1MNEE150 pKa = 3.47RR151 pKa = 11.84FIVKK155 pKa = 10.02KK156 pKa = 10.31KK157 pKa = 6.5WTTHH161 pKa = 4.89LMSTGTDD168 pKa = 3.28YY169 pKa = 11.51GGKK172 pKa = 7.53QTYY175 pKa = 8.75KK176 pKa = 10.53APSMPNYY183 pKa = 9.85KK184 pKa = 10.17KK185 pKa = 10.14PLNVNVRR192 pKa = 11.84NINVKK197 pKa = 7.63TVWKK201 pKa = 8.79DD202 pKa = 3.18TGGGKK207 pKa = 10.11YY208 pKa = 10.33EE209 pKa = 4.14DD210 pKa = 3.64VKK212 pKa = 11.28EE213 pKa = 3.89NAILYY218 pKa = 9.01VVVNDD223 pKa = 3.71NTDD226 pKa = 3.47NTNMYY231 pKa = 7.83ATLYY235 pKa = 9.93GNCRR239 pKa = 11.84CYY241 pKa = 10.29FYY243 pKa = 11.55

Molecular weight: 27.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1057

88

346

176.2

20.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.595 ± 0.631

2.176 ± 0.573

5.393 ± 0.586

5.487 ± 1.353

4.636 ± 1.082

5.487 ± 0.949

2.176 ± 0.629

6.528 ± 0.549

8.798 ± 0.964

6.623 ± 0.901

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.217 ± 1.131

5.393 ± 1.121

5.109 ± 0.848

4.636 ± 0.513

4.636 ± 0.772

7.758 ± 1.055

6.244 ± 1.749

5.771 ± 0.559

1.892 ± 0.316

4.447 ± 0.712
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski