Limnohabitans sp. TS-CS-82
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5751 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S7JL68|A0A2S7JL68_9BURK GntR family transcriptional regulator OS=Limnohabitans sp. TS-CS-82 OX=2094193 GN=C5F52_22940 PE=4 SV=1
MM1 pKa = 7.32 EE2 pKa = 6.79 LIMATAANNPSLILNISGTQSVFLKK27 pKa = 10.41 RR28 pKa = 11.84 GEE30 pKa = 4.19 KK31 pKa = 9.6 TSVIAKK37 pKa = 9.2 AGQRR41 pKa = 11.84 YY42 pKa = 8.43 RR43 pKa = 11.84 VVKK46 pKa = 10.2 EE47 pKa = 3.71 GEE49 pKa = 4.06 EE50 pKa = 3.99 AKK52 pKa = 10.52 EE53 pKa = 3.71 AAAKK57 pKa = 10.6 DD58 pKa = 3.82 SAAQAAKK65 pKa = 10.4 DD66 pKa = 3.72 VAASQQGQDD75 pKa = 4.12 LLLSYY80 pKa = 11.54 ADD82 pKa = 3.69 GTQVLLVSFYY92 pKa = 9.64 EE93 pKa = 4.03 ACKK96 pKa = 10.68 AEE98 pKa = 4.11 QCAVDD103 pKa = 3.72 MPGAKK108 pKa = 8.72 GTGTSGGYY116 pKa = 10.47 VITGDD121 pKa = 3.89 SAVGASLSDD130 pKa = 3.42 GGKK133 pKa = 9.78 LVYY136 pKa = 10.76 AFGDD140 pKa = 4.11 TPSMAALTQGAEE152 pKa = 3.87 QARR155 pKa = 11.84 GFSHH159 pKa = 6.77 QDD161 pKa = 2.95 SLSTYY166 pKa = 8.05 MPPSDD171 pKa = 3.39 GGFWTPLNVAFGALGVLGVATVLSSSKK198 pKa = 9.75 TEE200 pKa = 3.92 TVPTIIRR207 pKa = 11.84 GNVVAGPVVAGNGLEE222 pKa = 4.25 VVAYY226 pKa = 9.5 KK227 pKa = 10.78 ADD229 pKa = 3.6 GTVLVQGRR237 pKa = 11.84 VAADD241 pKa = 3.54 GSFEE245 pKa = 5.08 LNVPGGYY252 pKa = 10.01 LGPVLVRR259 pKa = 11.84 VHH261 pKa = 6.45 DD262 pKa = 3.97 TFAGSDD268 pKa = 3.84 YY269 pKa = 11.02 LDD271 pKa = 4.06 EE272 pKa = 4.58 ATSTLKK278 pKa = 10.86 DD279 pKa = 3.25 LTMDD283 pKa = 3.95 LRR285 pKa = 11.84 AATVISAPGIVKK297 pKa = 10.26 ININPITEE305 pKa = 3.98 LAVRR309 pKa = 11.84 KK310 pKa = 9.0 LALAGGDD317 pKa = 3.57 NANSEE322 pKa = 4.11 IALAGLTAQTIVSTNQQLAKK342 pKa = 10.84 ALGLPDD348 pKa = 4.1 GQDD351 pKa = 3.21 LVTGQAPVAVVDD363 pKa = 4.39 AQGHH367 pKa = 5.95 PNPATNDD374 pKa = 3.45 YY375 pKa = 10.73 GRR377 pKa = 11.84 LLAALSGAEE386 pKa = 4.0 AASGSNTVFDD396 pKa = 4.04 TLVANLTDD404 pKa = 3.67 NGLTAQAVDD413 pKa = 4.12 LLVNGAAQVSTTQSLDD429 pKa = 3.38 SDD431 pKa = 4.17 VKK433 pKa = 10.12 TLLNGALSAQEE444 pKa = 3.79 AMVYY448 pKa = 9.3 TLSNFDD454 pKa = 3.52 GTGDD458 pKa = 3.47 QPTIASYY465 pKa = 8.02 TTAGVTGVTTANLHH479 pKa = 6.43 VIDD482 pKa = 4.63 QAVATLNASSTDD494 pKa = 3.36 STAEE498 pKa = 3.82 LQALVDD504 pKa = 4.3 AYY506 pKa = 11.27 SAVLAVASTGGALTLGNFDD525 pKa = 4.24 ALGISGVTEE534 pKa = 4.63 DD535 pKa = 4.7 NLQAVQDD542 pKa = 4.18 ALAATADD549 pKa = 4.2 DD550 pKa = 4.84 GSDD553 pKa = 3.07 VDD555 pKa = 4.64 TLAKK559 pKa = 10.06 LQAVVDD565 pKa = 4.1 TAVALAWLSAYY576 pKa = 10.4 DD577 pKa = 4.03 GTDD580 pKa = 3.29 TAPTSLDD587 pKa = 3.21 YY588 pKa = 10.39 TAAGVTGVTDD598 pKa = 3.86 SNLDD602 pKa = 4.09 AINSALANLPANATGTAAQLQTVVSAYY629 pKa = 10.65 SAVLSAADD637 pKa = 4.93 GIAGNTTTAPTAAQYY652 pKa = 7.87 TALGVTGLTGNTASATASLLGNLIDD677 pKa = 4.61 GLSSADD683 pKa = 3.14 VDD685 pKa = 4.39 TVAEE689 pKa = 4.17 LQALANAASAVVAAASGGSVTLAQLQALGIMGVTADD725 pKa = 4.28 NLAAVQTALAATADD739 pKa = 4.26 DD740 pKa = 4.11 GTGVDD745 pKa = 4.64 SLTEE749 pKa = 3.71 IQALVTQAQAAFLAAQAVAALSAYY773 pKa = 10.33 DD774 pKa = 3.86 GTNTAPSLANYY785 pKa = 7.48 TAAGLAGVSSANLSAINSALAALPANATGTVAQLQTVVDD824 pKa = 4.95 AYY826 pKa = 9.93 TAVLATAEE834 pKa = 4.59 GTASSAPTAAQYY846 pKa = 10.86 AALGVTGLTGNTASATASLLGNLIDD871 pKa = 4.61 GLSSADD877 pKa = 3.14 VDD879 pKa = 4.39 TVAEE883 pKa = 4.17 LQALANAASAVVAAASGGSVTLAQLQALGITGVTADD919 pKa = 4.32 NLAAVQTALSATADD933 pKa = 3.85 DD934 pKa = 4.31 GTGVDD939 pKa = 4.46 SLIEE943 pKa = 3.83 IQALVGQVQAAVTAVQALATLSAYY967 pKa = 10.22 DD968 pKa = 4.22 GNTDD972 pKa = 3.47 APTSLDD978 pKa = 3.32 YY979 pKa = 10.4 TAAGVTGVTDD989 pKa = 3.86 SNLDD993 pKa = 4.09 AINSALANLPANATGTAAQLQIVVSAYY1020 pKa = 10.65 SAVLSAADD1028 pKa = 4.93 GIAGNTTTAPTAAQYY1043 pKa = 7.87 TALGVTGLTGNTASATASLLGNLIDD1068 pKa = 4.61 GLSSADD1074 pKa = 3.14 VDD1076 pKa = 4.39 TVAEE1080 pKa = 4.17 LQALANAASAVVGAASGGSVTLAQLQALGIMGVTADD1116 pKa = 4.28 NLAAVQTALAATADD1130 pKa = 4.26 DD1131 pKa = 4.11 GTGVDD1136 pKa = 4.64 SLTEE1140 pKa = 3.71 IQALVTQAEE1149 pKa = 4.53 SAFAAAQALAVLSGYY1164 pKa = 10.7 DD1165 pKa = 3.66 GTNTAPTSLDD1175 pKa = 3.17 YY1176 pKa = 10.39 TAAGVTGVTDD1186 pKa = 3.86 SNLDD1190 pKa = 4.09 AINSALANLPANATGTAAQLQIVVSAYY1217 pKa = 10.65 SAVLSAAYY1225 pKa = 9.74 GIAGNTTTAPTAAQYY1240 pKa = 7.87 TALGVTGLTGNTASATASLLGNLIDD1265 pKa = 4.75 GLSSTDD1271 pKa = 2.88 VDD1273 pKa = 4.26 TVAEE1277 pKa = 4.16 LQALANAASAVVGAASGGSVTLAQLQALGISGVTADD1313 pKa = 4.9 NLPTVQALLAATYY1326 pKa = 11.01 DD1327 pKa = 3.84 DD1328 pKa = 3.87 GTGVDD1333 pKa = 4.05 TLSEE1337 pKa = 3.93 IQTLVNTAAAVFTLSNYY1354 pKa = 10.49 SGTNTEE1360 pKa = 4.53 PDD1362 pKa = 3.4 AADD1365 pKa = 3.42 YY1366 pKa = 10.56 AAAGITGVIAGNLSAINSALAALPANATGTAAQLQTVVDD1405 pKa = 4.95 AYY1407 pKa = 9.91 TAVLAAANGTSTTAPTAAQFIALGVTVSGANNAAITSAASLLSSLIGEE1455 pKa = 4.62 LASADD1460 pKa = 3.4 VDD1462 pKa = 4.28 TVAEE1466 pKa = 4.17 LQALANAAAAVVAAAAGGTITLAQLQALGIDD1497 pKa = 3.76 VTHH1500 pKa = 7.28 ADD1502 pKa = 2.94 ITAVQTALAATADD1515 pKa = 4.17 DD1516 pKa = 4.49 GSGVSSLSALQTLVGNCQASNSGALNTISNYY1547 pKa = 10.64 NGTTNAPSAADD1558 pKa = 3.45 YY1559 pKa = 10.55 TNAFVTGVNANNLDD1573 pKa = 4.45 AINSAVAGLPNADD1586 pKa = 3.76 KK1587 pKa = 11.12 DD1588 pKa = 4.12 SAAEE1592 pKa = 3.94 LQSVVDD1598 pKa = 4.89 AYY1600 pKa = 10.04 TAVLAAADD1608 pKa = 4.08 GVAGNTTTAPNAAQYY1623 pKa = 7.75 TALGVTGLTGSTANATASLLGNLIDD1648 pKa = 4.73 GLTSADD1654 pKa = 3.2 VDD1656 pKa = 4.34 TVAEE1660 pKa = 4.26 LQTLANAAAAVVSAASGSPISLAQLKK1686 pKa = 10.38 ALGITGVTADD1696 pKa = 4.18 NLATVQAMLAATADD1710 pKa = 3.92 DD1711 pKa = 4.08 GTGVDD1716 pKa = 4.69 SLAEE1720 pKa = 3.96 IQTLANKK1727 pKa = 9.34 AAAVFTLSNYY1737 pKa = 8.48 TGSNTEE1743 pKa = 4.33 PEE1745 pKa = 4.11 AADD1748 pKa = 3.52 YY1749 pKa = 10.61 VAAGVTGVSSTNLSAINDD1767 pKa = 3.74 ALAALSASATGTPAALQTVVDD1788 pKa = 5.15 AYY1790 pKa = 10.96 AAVLAFADD1798 pKa = 4.58 GTSTTVPSASQYY1810 pKa = 8.41 TALGVTGMSGNSATATANLLGNLIGSLSRR1839 pKa = 11.84 ADD1841 pKa = 3.56 VNTVAEE1847 pKa = 4.27 IQALANAAAAVVAAASGTAISLVQLQALGITGVTANNLATVQAALAATADD1897 pKa = 4.33 DD1898 pKa = 4.81 GSGLDD1903 pKa = 3.88 TLSEE1907 pKa = 4.09 LQALVTPAVAVFTLSTYY1924 pKa = 10.04 TGGIPSPTAADD1935 pKa = 3.52 YY1936 pKa = 10.86 AAAGVTGVSDD1946 pKa = 3.7 INVSAINNALAVLPVSTTGTSHH1968 pKa = 8.0 DD1969 pKa = 3.94 LQTVVDD1975 pKa = 4.96 AYY1977 pKa = 11.28 SAVFSAAAGGSVTQAQLQALGITGVSADD2005 pKa = 3.78 NLAIVQALLSATADD2019 pKa = 3.73 DD2020 pKa = 4.79 GSGVDD2025 pKa = 4.89 SLTEE2029 pKa = 3.81 VQALVTQAAAVFTLSGYY2046 pKa = 8.8 TGANNAPTTADD2057 pKa = 3.34 YY2058 pKa = 11.02 AAAGVTGVNSANLSAINSALAEE2080 pKa = 4.3 LPASATGTIADD2091 pKa = 4.18 LQTVVDD2097 pKa = 4.98 AYY2099 pKa = 9.78 TAVFAAAAGGRR2110 pKa = 11.84 VSLAQLSALGVTGITADD2127 pKa = 4.18 NIGTVQALLDD2137 pKa = 3.64 ATADD2141 pKa = 3.85 DD2142 pKa = 4.7 GSGVDD2147 pKa = 4.01 TLSEE2151 pKa = 3.96 LQALVAKK2158 pKa = 10.83 AEE2160 pKa = 3.95 ALYY2163 pKa = 10.13 TLGQFDD2169 pKa = 4.41 GTNTAPVLASFTAAGVTGVSSTNLSAINSALANLPASASATVTSAEE2215 pKa = 4.1 LQTVVDD2221 pKa = 5.16 AYY2223 pKa = 10.95 AAVLAAADD2231 pKa = 4.16 GVAGNATTTPTAAQYY2246 pKa = 10.9 SALGLTGLTGLTGTSANATAALLGQLIDD2274 pKa = 4.12 GLSAADD2280 pKa = 3.42 VDD2282 pKa = 4.9 TVAEE2286 pKa = 4.17 LQALANAASAVVAAASGATISLAQLQTLGITGVTTDD2322 pKa = 3.91 NLATVQALLAATADD2336 pKa = 4.07 DD2337 pKa = 4.1 GTGVDD2342 pKa = 4.69 SLAEE2346 pKa = 3.79 IQILVNQAAAVFTLSSYY2363 pKa = 10.48 TGTNTEE2369 pKa = 4.29 PQAADD2374 pKa = 3.29 YY2375 pKa = 8.05 TAAGVSGVTAANLVAINSALAALPATATGSVAALQSVADD2414 pKa = 4.54 AYY2416 pKa = 11.33 NAVLSAANGTSTTAPTPAQLATLGVNVPGASTAVAASATALLCSLIGGLQAADD2469 pKa = 3.29 VDD2471 pKa = 4.85 TVAEE2475 pKa = 4.17 LQALANAAAAVVAAAAGGSITLAQLQALGINAANVDD2511 pKa = 3.4 ITTVNTALAATADD2524 pKa = 4.06 DD2525 pKa = 4.52 GAGVASLSALQTLVNNCVDD2544 pKa = 3.4 SSGGPLARR2552 pKa = 11.84 ITNYY2556 pKa = 10.13 TGITDD2561 pKa = 3.96 APSVANYY2568 pKa = 7.67 ATAGVTGVTDD2578 pKa = 4.07 SNIKK2582 pKa = 10.24 AINSVVAGLPTASKK2596 pKa = 10.81 DD2597 pKa = 3.7 SASEE2601 pKa = 3.94 LQTLVDD2607 pKa = 4.43 AYY2609 pKa = 10.86 AAISATADD2617 pKa = 3.3 GTAGNATNLPTAAQYY2632 pKa = 7.87 TALGVTGLSGNTANATAALLGNLIDD2657 pKa = 5.4 GLTQADD2663 pKa = 3.77 VDD2665 pKa = 4.56 TVAEE2669 pKa = 4.17 LQALADD2675 pKa = 3.8 AARR2678 pKa = 11.84 SVVAAASGTPITLDD2692 pKa = 3.39 PLKK2695 pKa = 10.82 ALGIIGLTADD2705 pKa = 3.8 NLVTVQALLAATADD2719 pKa = 4.07 DD2720 pKa = 4.03 GTGVDD2725 pKa = 4.13 TLSEE2729 pKa = 3.92 IQTLVNKK2736 pKa = 9.96 AAALFTLSSYY2746 pKa = 11.35 NGTNTEE2752 pKa = 4.34 PQTGDD2757 pKa = 3.24 YY2758 pKa = 7.39 TTAGVTGVSDD2768 pKa = 4.31 ANVSAINSALAGLDD2782 pKa = 3.29 TTATDD2787 pKa = 3.34 STADD2791 pKa = 3.43 VQAVVDD2797 pKa = 4.97 AYY2799 pKa = 10.94 AAVLAAADD2807 pKa = 4.11 GTSAMSQALPTAAQYY2822 pKa = 7.87 TALGVTGLTGTTANATAALLGNRR2845 pKa = 11.84 IDD2847 pKa = 4.11 SLSSADD2853 pKa = 3.24 VDD2855 pKa = 4.34 TVAEE2859 pKa = 4.17 LQALANAAAAVVSAASGGSITLAQVQALGITGVTADD2895 pKa = 4.18 NLATVQAALAATNDD2909 pKa = 4.17 DD2910 pKa = 3.72 GTGLDD2915 pKa = 3.88 TLAEE2919 pKa = 4.09 IQTLVTQAAAVYY2931 pKa = 8.52 TLSAYY2936 pKa = 9.33 TGTNTVPKK2944 pKa = 9.64 TGDD2947 pKa = 3.12 YY2948 pKa = 8.67 TAAGVTGVSDD2958 pKa = 4.31 ANVSAINSALAALPASATDD2977 pKa = 4.2 SVAEE2981 pKa = 4.11 LQTVVDD2987 pKa = 4.73 AYY2989 pKa = 11.14 VAVLAAADD2997 pKa = 4.43 RR2998 pKa = 11.84 EE2999 pKa = 4.29 PGNLGAAAPTLAQYY3013 pKa = 10.84 NVLGITGMSTQGSAKK3028 pKa = 10.32 ASLLNDD3034 pKa = 3.58 VIDD3037 pKa = 4.05 RR3038 pKa = 11.84 RR3039 pKa = 11.84 AATDD3043 pKa = 2.92 VDD3045 pKa = 4.53 TVAGIQWLANITNALVDD3062 pKa = 4.41 DD3063 pKa = 5.14 ASSATPALNKK3073 pKa = 8.92 TQVDD3077 pKa = 3.66 TLGRR3081 pKa = 11.84 IVGGITKK3088 pKa = 10.15 EE3089 pKa = 3.85 NLSIFNKK3096 pKa = 9.82 VVTATADD3103 pKa = 3.25 NGSGVDD3109 pKa = 3.54 TLVEE3113 pKa = 4.13 VQNLVQVAVEE3123 pKa = 4.02 LKK3125 pKa = 10.33 KK3126 pKa = 11.05 LSVYY3130 pKa = 10.56 SGTVRR3135 pKa = 11.84 APSLATYY3142 pKa = 9.37 QAAGVTGVTEE3152 pKa = 4.53 GNLSAINTVIAHH3164 pKa = 6.77 LPSTGTDD3171 pKa = 2.88 EE3172 pKa = 4.13 ATEE3175 pKa = 3.94 VQIAVDD3181 pKa = 4.11 AYY3183 pKa = 10.81 SAVLAAADD3191 pKa = 3.97 GTAGNATGVITASQYY3206 pKa = 11.1 DD3207 pKa = 3.79 ALGVIGMGTSAARR3220 pKa = 11.84 RR3221 pKa = 11.84 SLMSSVVDD3229 pKa = 3.49 ARR3231 pKa = 11.84 QATDD3235 pKa = 2.66 VDD3237 pKa = 4.66 TVAEE3241 pKa = 4.14 LQALGTAVTAVITAAAGTTTDD3262 pKa = 3.02 VTLAGLQSLGITGVTSANLAAVQTALAASPDD3293 pKa = 4.06 DD3294 pKa = 4.02 GSGVDD3299 pKa = 4.02 TLTKK3303 pKa = 10.47 LQALVTGVVQQANNSNANPSIPTPVALSDD3332 pKa = 3.14 IAAGVGGFVINGQGATDD3349 pKa = 3.78 GNGYY3353 pKa = 9.78 SVSAAGDD3360 pKa = 3.58 VNGDD3364 pKa = 3.37 GLADD3368 pKa = 4.01 VIVGAPYY3375 pKa = 10.14 ANEE3378 pKa = 3.93 RR3379 pKa = 11.84 VGRR3382 pKa = 11.84 SYY3384 pKa = 11.81 VVFGTTAGTTIDD3396 pKa = 4.32 LSAVAAGTGGFVINGQSVSGQAAEE3420 pKa = 4.26 SGKK3423 pKa = 10.42 SVSAAGDD3430 pKa = 3.54 VNGDD3434 pKa = 3.52 GLADD3438 pKa = 5.2 LIVGANGVNYY3448 pKa = 10.27 SAGSSYY3454 pKa = 11.07 VVYY3457 pKa = 10.55 GKK3459 pKa = 9.07 TSGLAIDD3466 pKa = 5.26 LSTVDD3471 pKa = 3.67 GGSGGFVLHH3480 pKa = 6.33 GQNVTDD3486 pKa = 3.55 KK3487 pKa = 10.86 SGYY3490 pKa = 8.79 SVSAAGDD3497 pKa = 3.58 VNGDD3501 pKa = 3.52 GLADD3505 pKa = 5.18 LIVGAYY3511 pKa = 7.88 YY3512 pKa = 11.24 ANVFGGRR3519 pKa = 11.84 SYY3521 pKa = 11.82 VVFGKK3526 pKa = 7.6 TTGTGMDD3533 pKa = 4.21 LSAVAGGAGGFVINGDD3549 pKa = 3.41 SSSYY3553 pKa = 9.75 FSGYY3557 pKa = 9.75 SVSAAGDD3564 pKa = 3.58 VNGDD3568 pKa = 3.52 GLADD3572 pKa = 5.05 LIVGAKK3578 pKa = 8.86 NANSGGGRR3586 pKa = 11.84 SYY3588 pKa = 11.76 VVFGKK3593 pKa = 7.6 TTGTGMDD3600 pKa = 4.21 LSAVAGGAGGFVINSDD3616 pKa = 2.93 SSTAEE3621 pKa = 3.46 NSGYY3625 pKa = 10.3 SVSAAGDD3632 pKa = 3.58 VNGDD3636 pKa = 3.52 GLADD3640 pKa = 5.2 LIVGAPYY3647 pKa = 11.0 SNDD3650 pKa = 3.03 GGGRR3654 pKa = 11.84 SYY3656 pKa = 11.78 VVFGKK3661 pKa = 7.58 TTGTVMDD3668 pKa = 4.97 LSAVAGGVGGFVINGDD3684 pKa = 3.41 SSSYY3688 pKa = 10.53 FSGNSVSAAGDD3699 pKa = 3.55 VNGDD3703 pKa = 3.52 GLADD3707 pKa = 5.25 LIVGAPNANGNSGRR3721 pKa = 11.84 SYY3723 pKa = 11.2 VVFGKK3728 pKa = 7.6 TTGTGMDD3735 pKa = 4.26 LSAVASGAGGFVINGDD3751 pKa = 3.9 SPLSFSGNSVSAAGDD3766 pKa = 3.55 VNGDD3770 pKa = 3.52 GLADD3774 pKa = 5.21 LIVGAYY3780 pKa = 9.01 NAIGGSGRR3788 pKa = 11.84 SYY3790 pKa = 11.28 VIFGNTTGAFGQTAVDD3806 pKa = 3.4 WMGTAGDD3813 pKa = 4.58 DD3814 pKa = 3.45 THH3816 pKa = 8.38 ADD3818 pKa = 3.21 NGTASTLAAGAGNDD3832 pKa = 3.94 TLTATAGSILLGGAGNDD3849 pKa = 3.34 NFNINAAMITALQSPLGSGGNTAQLARR3876 pKa = 11.84 IDD3878 pKa = 4.13 GGSGIDD3884 pKa = 3.49 TVALSGTGLTLDD3896 pKa = 4.15 LTQVANQAAANPDD3909 pKa = 3.16 GGSRR3913 pKa = 11.84 IDD3915 pKa = 3.46 SAEE3918 pKa = 4.22 TIDD3921 pKa = 5.04 LTGTGNNSLTLAARR3935 pKa = 11.84 DD3936 pKa = 3.94 VVDD3939 pKa = 3.54 MAGMNTFNSSNGWTSLGATVQAHH3962 pKa = 4.52 QLLVKK3967 pKa = 10.57 GDD3969 pKa = 3.74 AGDD3972 pKa = 3.52 VLNAQGTWYY3981 pKa = 10.86 NDD3983 pKa = 3.29 GTVSQGGQTYY3993 pKa = 10.22 QIYY3996 pKa = 10.38 NAIDD4000 pKa = 3.24 SAAQMLVDD4008 pKa = 3.72 TDD4010 pKa = 3.05 ITRR4013 pKa = 11.84 NVTVTPRR4020 pKa = 11.84 IYY4022 pKa = 10.22 PVNLSDD4028 pKa = 3.31 IAAGVGGFVINGDD4041 pKa = 3.95 SSTSTYY4047 pKa = 10.77 SGYY4050 pKa = 10.67 SVSAAGDD4057 pKa = 3.58 VNGDD4061 pKa = 3.52 GLADD4065 pKa = 5.25 LIVGAPNANSSRR4077 pKa = 11.84 GRR4079 pKa = 11.84 SYY4081 pKa = 11.64 VVFGKK4086 pKa = 7.61 TTGTGLNLSAVAGGAGGFVINGDD4109 pKa = 3.81 STSTSSGYY4117 pKa = 10.33 SVSAAGDD4124 pKa = 3.58 VNGDD4128 pKa = 3.52 GLADD4132 pKa = 5.22 LIVGAYY4138 pKa = 8.23 RR4139 pKa = 11.84 ANSGGGRR4146 pKa = 11.84 SYY4148 pKa = 11.76 VVFGKK4153 pKa = 7.6 TTGTGMDD4160 pKa = 4.21 LSAVAGGAGGFVISGDD4176 pKa = 3.24 SSTAEE4181 pKa = 3.51 NSGYY4185 pKa = 10.3 SVSAAGDD4192 pKa = 3.58 VNGDD4196 pKa = 3.52 GLADD4200 pKa = 5.28 LIVGAYY4206 pKa = 8.21 GANSGGGRR4214 pKa = 11.84 SYY4216 pKa = 11.76 VVFGKK4221 pKa = 7.5 TTATGMNLSAVAGGAGGFVINGDD4244 pKa = 3.22 NSSIYY4249 pKa = 10.65 SGASVSAAGDD4259 pKa = 3.58 VNGDD4263 pKa = 3.52 GLADD4267 pKa = 5.28 LIVGAYY4273 pKa = 8.21 GANSGGGRR4281 pKa = 11.84 SYY4283 pKa = 11.76 VVFGKK4288 pKa = 7.61 TTGTGLNLSAVAGGAGGFVINGDD4311 pKa = 3.81 STSTSSGYY4319 pKa = 10.33 SVSAAGDD4326 pKa = 3.58 VNGDD4330 pKa = 3.52 GLADD4334 pKa = 5.29 LIVGAPDD4341 pKa = 3.51 ANSGGGRR4348 pKa = 11.84 SYY4350 pKa = 11.76 VVFGKK4355 pKa = 7.6 TTGTGMDD4362 pKa = 4.21 LSAVAGGAGGFVINGDD4378 pKa = 3.81 STSTSSGWSVSAAGDD4393 pKa = 3.64 VNGDD4397 pKa = 3.52 GLADD4401 pKa = 5.22 LIVGAYY4407 pKa = 8.23 RR4408 pKa = 11.84 ANSGGGRR4415 pKa = 11.84 SYY4417 pKa = 11.76 VVFGKK4422 pKa = 7.6 TTGTGMDD4429 pKa = 4.21 LSAVAGGAGGFVINGDD4445 pKa = 3.81 STSTSSGWSVSAAGDD4460 pKa = 3.64 VNGDD4464 pKa = 3.52 GLADD4468 pKa = 5.29 LIVGTPDD4475 pKa = 3.06 ANSNGGRR4482 pKa = 11.84 SYY4484 pKa = 11.5 VIFGNTTGAFNSSAVDD4500 pKa = 3.34 WVGTAGTDD4508 pKa = 3.28 SRR4510 pKa = 11.84 SDD4512 pKa = 3.16 NGTASTLAAGAGNDD4526 pKa = 3.74 TLTATAASSLLGGAGNDD4543 pKa = 3.43 TFIINATMVTALQSPLGSGGNISQLARR4570 pKa = 11.84 IDD4572 pKa = 3.83 GGTGQDD4578 pKa = 3.94 TIALDD4583 pKa = 3.72 AAGLNLVLGQIANKK4597 pKa = 9.11 TDD4599 pKa = 3.27 GVNQTDD4605 pKa = 3.39 GRR4607 pKa = 11.84 INSVEE4612 pKa = 4.26 RR4613 pKa = 11.84 IDD4615 pKa = 4.0 ITGTGANILNVTASDD4630 pKa = 3.74 VRR4632 pKa = 11.84 DD4633 pKa = 3.54 MAGMNSINTTTGSGWSDD4650 pKa = 3.04 GTYY4653 pKa = 10.08 TLGAAVSRR4661 pKa = 11.84 HH4662 pKa = 4.64 QLVIDD4667 pKa = 4.3 GNTGDD4672 pKa = 3.89 MARR4675 pKa = 11.84 LTDD4678 pKa = 4.29 SGWTLMGTALKK4689 pKa = 9.17 GTATYY4694 pKa = 10.42 KK4695 pKa = 10.56 VYY4697 pKa = 11.27 NSASGLAQVLVANAVTVVNSSEE4719 pKa = 3.94 INVFSMSGLAGNTGGFIINGQAAGDD4744 pKa = 3.6 EE4745 pKa = 4.63 AGFSVASAGDD4755 pKa = 3.59 VNGDD4759 pKa = 3.76 GLVDD4763 pKa = 4.17 MIIGAHH4769 pKa = 6.59 KK4770 pKa = 10.75 ADD4772 pKa = 3.72 ANGNNDD4778 pKa = 2.98 AGRR4781 pKa = 11.84 SYY4783 pKa = 11.69 VVFGTTSKK4791 pKa = 11.15 AAINLSALAATAGGTGGFVINGQGGADD4818 pKa = 3.28 EE4819 pKa = 4.75 SGYY4822 pKa = 10.68 SVSAAGDD4829 pKa = 3.58 VNGDD4833 pKa = 3.52 GLADD4837 pKa = 5.23 LIVGAPLSDD4846 pKa = 3.93 GNGNDD4851 pKa = 3.16 AGRR4854 pKa = 11.84 TYY4856 pKa = 11.44 VVFGKK4861 pKa = 9.27 TNTTPINLSAVAGNTGGFAIAGPAGYY4887 pKa = 9.21 DD4888 pKa = 3.14 QSGYY4892 pKa = 10.61 SVASAGDD4899 pKa = 3.57 VNGDD4903 pKa = 3.48 GLADD4907 pKa = 5.2 LIVGVPMADD4916 pKa = 3.02 GSSTNSSTNDD4926 pKa = 2.79 AGRR4929 pKa = 11.84 AYY4931 pKa = 10.83 VVFGKK4936 pKa = 7.25 TTAGLVSLSALGTGGFAINNMHH4958 pKa = 6.54 GFDD4961 pKa = 3.76 YY4962 pKa = 10.91 VGNSVASAGDD4972 pKa = 3.54 VNGDD4976 pKa = 3.32 GLADD4980 pKa = 3.5 VLISAKK4986 pKa = 10.54 CADD4989 pKa = 3.65 ANGNNDD4995 pKa = 3.18 AGQTFVIYY5003 pKa = 10.13 GQTGNAAITLSALGSSGYY5021 pKa = 10.29 VINGQDD5027 pKa = 3.72 AYY5029 pKa = 10.89 DD5030 pKa = 3.58 EE5031 pKa = 4.85 SGTNVANAGDD5041 pKa = 3.88 VNGDD5045 pKa = 3.73 GLSDD5049 pKa = 4.73 LIIGAPHH5056 pKa = 6.3 PTGTVNAGRR5065 pKa = 11.84 SYY5067 pKa = 11.58 VVFGQTGTPSAINLSAVAGGTGGFVIVGQQNSDD5100 pKa = 3.05 RR5101 pKa = 11.84 AGYY5104 pKa = 9.7 SVASAGDD5111 pKa = 3.64 FNGDD5115 pKa = 3.26 GLADD5119 pKa = 4.98 LIIGAPLVDD5128 pKa = 4.4 AQPGTNNANDD5138 pKa = 3.68 AGRR5141 pKa = 11.84 SYY5143 pKa = 11.73 VVFGQTGTAPINLSAVAAGRR5163 pKa = 11.84 GGFVLSGVTGADD5175 pKa = 3.1 QVGYY5179 pKa = 10.29 SVANAGDD5186 pKa = 3.63 INGDD5190 pKa = 3.52 GLTDD5194 pKa = 5.41 LIVGAQGVDD5203 pKa = 3.18 ANGNDD5208 pKa = 3.31 AGRR5211 pKa = 11.84 AYY5213 pKa = 10.75 VILGNSQGALLKK5225 pKa = 8.59 MQPDD5229 pKa = 3.66 QLGTSAADD5237 pKa = 3.48 TLTATAASVLYY5248 pKa = 10.6 GGAGNDD5254 pKa = 3.12 RR5255 pKa = 11.84 FVLGTDD5261 pKa = 2.98 MLTALEE5267 pKa = 4.5 SPMGSGGNTTQLARR5281 pKa = 11.84 IDD5283 pKa = 3.88 GGVGVDD5289 pKa = 3.36 TLALNGSGQHH5299 pKa = 6.68 LDD5301 pKa = 3.29 LARR5304 pKa = 11.84 IANTLGSSRR5313 pKa = 11.84 LNSIEE5318 pKa = 4.3 VVDD5321 pKa = 4.68 LTGTGNNMITPTVADD5336 pKa = 3.86 VVAMNGVNVFNTGNGWTGTGLSASVMRR5363 pKa = 11.84 HH5364 pKa = 4.54 QLRR5367 pKa = 11.84 IDD5369 pKa = 3.81 GNSGDD5374 pKa = 4.41 SGDD5377 pKa = 4.29 SVNLPSGWTNTNTTATLAGQTYY5399 pKa = 11.02 NLYY5402 pKa = 10.64 QSTNGQAQLLVQSTVTVAPDD5422 pKa = 3.22 VAPTLSSVALTASSGPQQAGFLKK5445 pKa = 10.84 AGATVTATVSFSEE5458 pKa = 4.25 DD5459 pKa = 3.22 LVVNTSAGSPTLALDD5474 pKa = 3.48 VGGTTVQATYY5484 pKa = 11.27 VGMSGKK5490 pKa = 10.09 QISFTTTIAAGQADD5504 pKa = 4.18 ADD5506 pKa = 4.78 GIALAANGLSLNGAVISDD5524 pKa = 3.78 TTGNAASALGYY5535 pKa = 10.27 SAVADD5540 pKa = 3.59 NSSYY5544 pKa = 11.15 KK5545 pKa = 10.19 VDD5547 pKa = 4.03 ALASTPNATANAGVLVAPTNAEE5569 pKa = 3.5 AFATVEE5575 pKa = 3.88 YY5576 pKa = 10.31 RR5577 pKa = 11.84 IKK5579 pKa = 10.02 TLAGTFGSWSSTFAQPATNGTADD5602 pKa = 2.82 GRR5604 pKa = 11.84 YY5605 pKa = 8.39 QVEE5608 pKa = 4.27 VRR5610 pKa = 11.84 QTDD5613 pKa = 3.15 AAGNVSAVQALTLDD5627 pKa = 3.31 VGRR5630 pKa = 11.84 FYY5632 pKa = 11.52 LPIDD5636 pKa = 4.23 LSDD5639 pKa = 3.22 ISAGVGGFVINGDD5652 pKa = 3.78 STSLFSGISVSAAGDD5667 pKa = 3.59 VNGDD5671 pKa = 3.52 GLADD5675 pKa = 5.16 LIVGAWGANSYY5686 pKa = 10.5 GGRR5689 pKa = 11.84 SYY5691 pKa = 11.78 VVFGKK5696 pKa = 7.6 TTGTGMDD5703 pKa = 4.21 LSAVAGGTGGFVINGDD5719 pKa = 3.1 ITSTYY5724 pKa = 10.32 SGLSVSSAGDD5734 pKa = 3.4 VNGDD5738 pKa = 3.48 GLADD5742 pKa = 5.13 LIVGATAANSNGGRR5756 pKa = 11.84 SYY5758 pKa = 11.72 VVFGKK5763 pKa = 7.6 TTGTGIALSDD5773 pKa = 4.15 LSPPAGPVGPAGGAGGFVINGDD5795 pKa = 3.57 YY5796 pKa = 10.86 SSYY5799 pKa = 10.89 RR5800 pKa = 11.84 SGNSVSAAGDD5810 pKa = 3.55 VNGDD5814 pKa = 3.52 GLADD5818 pKa = 5.09 LIVGANAANSTNGRR5832 pKa = 11.84 SYY5834 pKa = 11.56 VVFGKK5839 pKa = 7.49 TTGAGMDD5846 pKa = 4.03 LSAVAGGAGGFVINGDD5862 pKa = 3.73 SSSSNSGNSVSAAGDD5877 pKa = 3.55 VNGDD5881 pKa = 3.52 GLADD5885 pKa = 5.14 LIVGALTANGYY5896 pKa = 10.3 AGRR5899 pKa = 11.84 SYY5901 pKa = 11.61 VVFGKK5906 pKa = 7.11 TTGMPMDD5913 pKa = 4.64 LSAVAGGTGGFVINGDD5929 pKa = 3.44 SSSAYY5934 pKa = 10.02 SGSSVSAAGDD5944 pKa = 3.56 VNGDD5948 pKa = 3.52 GLADD5952 pKa = 5.25 LIVGAPNANSGGGRR5966 pKa = 11.84 SYY5968 pKa = 11.76 VVFGKK5973 pKa = 7.59 TTGTGIDD5980 pKa = 3.85 LSAVAGGAGGFVINGDD5996 pKa = 3.6 SSSTKK6001 pKa = 9.92 SGYY6004 pKa = 9.93 SVSAAGDD6011 pKa = 3.58 VNGDD6015 pKa = 3.37 GLADD6019 pKa = 3.99 VIVGAWNANSGGGRR6033 pKa = 11.84 SYY6035 pKa = 11.76 VVFGKK6040 pKa = 7.6 TTGTGMDD6047 pKa = 4.26 LSAVASGAGGFVINGDD6063 pKa = 3.58 SSSANSGWSVSAAGDD6078 pKa = 3.64 VNGDD6082 pKa = 3.52 GLADD6086 pKa = 5.02 LIVGAKK6092 pKa = 9.29 AANSDD6097 pKa = 3.47 GGRR6100 pKa = 11.84 SYY6102 pKa = 11.54 VIFGNTSGAFNSSAVDD6118 pKa = 3.42 WVGTAGDD6125 pKa = 4.43 DD6126 pKa = 3.48 THH6128 pKa = 8.38 ADD6130 pKa = 3.21 NGTASTLAAGAGNDD6144 pKa = 3.74 TLTATAASVLYY6155 pKa = 10.56 GGAGNDD6161 pKa = 3.41 TFNINDD6167 pKa = 3.69 TMIGALLRR6175 pKa = 11.84 PMGSGGNTTQLARR6188 pKa = 11.84 IDD6190 pKa = 3.86 GGGGLDD6196 pKa = 3.47 TIVLNVSGLTIDD6208 pKa = 4.01 LTRR6211 pKa = 11.84 VANQAASNPDD6221 pKa = 2.92 GGSRR6225 pKa = 11.84 IDD6227 pKa = 3.88 SIEE6230 pKa = 4.51 KK6231 pKa = 9.83 IDD6233 pKa = 3.61 ITGSGDD6239 pKa = 3.29 NTLVLQLRR6247 pKa = 11.84 DD6248 pKa = 3.79 VLDD6251 pKa = 3.66 MGSANLFQTTGRR6263 pKa = 11.84 QQLLVKK6269 pKa = 10.38 GNAGDD6274 pKa = 3.97 TVDD6277 pKa = 4.78 LADD6280 pKa = 4.71 GSGTTGWTHH6289 pKa = 6.23 TATNATIDD6297 pKa = 3.74 SLSYY6301 pKa = 10.17 EE6302 pKa = 4.36 VWNHH6306 pKa = 5.57 DD6307 pKa = 3.29 TSKK6310 pKa = 10.07 ATLYY6314 pKa = 9.9 IQTGVVVGG6322 pKa = 4.0
Molecular weight: 612.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.567
IPC_protein 3.656
Toseland 3.401
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.643
Rodwell 3.465
Grimsley 3.3
Solomon 3.668
Lehninger 3.63
Nozaki 3.77
DTASelect 4.113
Thurlkill 3.465
EMBOSS 3.643
Sillero 3.783
Patrickios 1.532
IPC_peptide 3.668
IPC2_peptide 3.757
IPC2.peptide.svr19 3.719
Protein with the highest isoelectric point:
>tr|A0A2S7JRD7|A0A2S7JRD7_9BURK Protein adenylyltransferase SelO OS=Limnohabitans sp. TS-CS-82 OX=2094193 GN=selO PE=3 SV=1
MM1 pKa = 7.57 MLRR4 pKa = 11.84 LLPTIAALIGGFLPRR19 pKa = 11.84 FIWTLPSLSMPSVGGFGGRR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 10.37 GSNAAIKK47 pKa = 10.39 RR48 pKa = 11.84 QTTKK52 pKa = 10.21 RR53 pKa = 11.84 RR54 pKa = 11.84 NIAKK58 pKa = 10.04 RR59 pKa = 11.84 LPLRR63 pKa = 11.84 KK64 pKa = 9.27 RR65 pKa = 11.84 RR66 pKa = 3.69
Molecular weight: 7.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.51
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.184
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5751
0
5751
1932117
28
20618
336.0
36.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.79 ± 0.075
0.922 ± 0.018
5.304 ± 0.031
5.121 ± 0.057
3.595 ± 0.033
7.88 ± 0.075
2.389 ± 0.034
4.634 ± 0.027
4.196 ± 0.045
10.34 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.732 ± 0.034
3.342 ± 0.044
4.624 ± 0.044
4.413 ± 0.036
5.447 ± 0.075
5.789 ± 0.064
5.912 ± 0.12
7.898 ± 0.036
1.415 ± 0.021
2.26 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here