Halarcobacter bivalviorum
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2581 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345IVM7|A0A345IVM7_9PROT NMN amidohydrolase OS=Halarcobacter bivalviorum OX=663364 GN=pncC PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.26 KK3 pKa = 10.6 FSLFTCALLLSTSTVFAADD22 pKa = 4.16 SIDD25 pKa = 3.42 EE26 pKa = 4.27 AFKK29 pKa = 10.74 SGKK32 pKa = 9.81 VSGDD36 pKa = 3.03 ITLHH40 pKa = 4.52 TVKK43 pKa = 10.38 YY44 pKa = 9.46 DD45 pKa = 3.4 NKK47 pKa = 10.53 GVVDD51 pKa = 3.78 SGFTAGTVGLAYY63 pKa = 7.91 EE64 pKa = 4.28 TGSFYY69 pKa = 11.28 GLSAKK74 pKa = 9.73 MGFRR78 pKa = 11.84 ANHH81 pKa = 5.85 EE82 pKa = 4.01 FSEE85 pKa = 4.54 VEE87 pKa = 3.74 EE88 pKa = 4.47 GDD90 pKa = 3.66 YY91 pKa = 11.62 EE92 pKa = 4.64 NFFVHH97 pKa = 7.04 DD98 pKa = 4.17 ALMTEE103 pKa = 5.13 AYY105 pKa = 9.88 LKK107 pKa = 10.36 YY108 pKa = 10.33 ASEE111 pKa = 4.45 SFSITAGRR119 pKa = 11.84 QAIDD123 pKa = 3.98 LEE125 pKa = 4.2 WLGDD129 pKa = 3.67 YY130 pKa = 11.21 NEE132 pKa = 5.59 AIVAAITAIPDD143 pKa = 3.33 TTIILGYY150 pKa = 7.64 TDD152 pKa = 3.75 RR153 pKa = 11.84 QAISDD158 pKa = 4.02 EE159 pKa = 4.67 DD160 pKa = 3.8 EE161 pKa = 4.87 SSDD164 pKa = 3.64 FTEE167 pKa = 4.37 LTNEE171 pKa = 3.85 GAYY174 pKa = 10.74 VLDD177 pKa = 3.56 IKK179 pKa = 11.33 YY180 pKa = 10.44 KK181 pKa = 10.87 AFDD184 pKa = 3.46 SLEE187 pKa = 4.16 FNPYY191 pKa = 10.3 AYY193 pKa = 10.14 SAPDD197 pKa = 3.18 AVDD200 pKa = 4.56 FYY202 pKa = 11.69 GLKK205 pKa = 9.71 TSYY208 pKa = 11.09 SSDD211 pKa = 2.74 IFSAIAHH218 pKa = 5.86 YY219 pKa = 8.07 ATSNVDD225 pKa = 3.72 SKK227 pKa = 11.19 MKK229 pKa = 10.6 DD230 pKa = 2.63 ITTDD234 pKa = 3.15 DD235 pKa = 3.97 GNILNLEE242 pKa = 4.6 LGTSFAGISASLGYY256 pKa = 10.31 IKK258 pKa = 9.72 TDD260 pKa = 2.8 KK261 pKa = 10.98 DD262 pKa = 3.6 YY263 pKa = 11.76 GIGLMDD269 pKa = 3.98 TYY271 pKa = 11.42 GDD273 pKa = 4.57 NINLMDD279 pKa = 5.23 SGNQIYY285 pKa = 10.43 SADD288 pKa = 3.5 AKK290 pKa = 9.66 TVYY293 pKa = 10.6 GSLSYY298 pKa = 10.59 EE299 pKa = 3.8 IAGVEE304 pKa = 4.02 LGALYY309 pKa = 10.84 SDD311 pKa = 4.12 TEE313 pKa = 4.24 YY314 pKa = 11.36 GADD317 pKa = 3.4 NFDD320 pKa = 3.72 EE321 pKa = 5.36 KK322 pKa = 11.12 EE323 pKa = 4.08 LNLTVSYY330 pKa = 10.79 AITDD334 pKa = 3.86 SLSVGLLYY342 pKa = 10.83 ADD344 pKa = 4.84 IDD346 pKa = 4.13 TDD348 pKa = 5.13 SDD350 pKa = 3.97 DD351 pKa = 5.04 ADD353 pKa = 4.93 SNDD356 pKa = 3.25 STYY359 pKa = 11.43 GSLTLSYY366 pKa = 11.21 SFF368 pKa = 4.92
Molecular weight: 39.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.202
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.961
Patrickios 0.553
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|A0A345IZL4|A0A345IZL4_9PROT DUF59 domain-containing protein OS=Halarcobacter bivalviorum OX=663364 GN=ABIV_2021 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 6.08 GFRR19 pKa = 11.84 IRR21 pKa = 11.84 MKK23 pKa = 9.04 TKK25 pKa = 10.16 NGRR28 pKa = 11.84 KK29 pKa = 8.88 VIKK32 pKa = 10.14 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.79 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2581
0
2581
831417
37
8997
322.1
36.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.909 ± 0.053
0.861 ± 0.02
5.682 ± 0.128
7.649 ± 0.067
5.267 ± 0.059
5.311 ± 0.114
1.561 ± 0.022
9.351 ± 0.057
9.587 ± 0.101
9.836 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.231 ± 0.034
6.197 ± 0.068
2.619 ± 0.037
2.758 ± 0.026
2.901 ± 0.034
6.557 ± 0.047
5.171 ± 0.095
5.854 ± 0.038
0.677 ± 0.017
4.02 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here