Capybara microvirus Cap1_SP_175
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVQ7|A0A4V1FVQ7_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_175 OX=2585404 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.17 GEE4 pKa = 4.04 KK5 pKa = 8.49 MGNVIGTVIGGLASNVSNATQGALSDD31 pKa = 3.73 SSSWGGGVSNSSSSSWEE48 pKa = 4.05 SGGSSSMNSASSAEE62 pKa = 4.41 DD63 pKa = 2.91 AWSQAISNSWTEE75 pKa = 3.94 ANEE78 pKa = 3.94 ANQNAAYY85 pKa = 9.56 AAYY88 pKa = 10.04 VANQLQDD95 pKa = 2.52 KK96 pKa = 9.43 WYY98 pKa = 7.84 QQQMEE103 pKa = 4.57 YY104 pKa = 10.76 NRR106 pKa = 11.84 QEE108 pKa = 3.99 AQNQRR113 pKa = 11.84 DD114 pKa = 3.72 FEE116 pKa = 4.28 EE117 pKa = 5.13 RR118 pKa = 11.84 MSSTAYY124 pKa = 9.76 QRR126 pKa = 11.84 AVNDD130 pKa = 4.06 LKK132 pKa = 11.26 AAGLNPILAATLGGSSTPSTSAASISSPTAKK163 pKa = 10.68 AADD166 pKa = 3.86 TYY168 pKa = 10.03 MNSYY172 pKa = 10.76 SSAKK176 pKa = 10.04 SASQSTGRR184 pKa = 11.84 SYY186 pKa = 11.43 SYY188 pKa = 11.43 GEE190 pKa = 4.48 SSSGWSGGSEE200 pKa = 4.26 STSHH204 pKa = 6.34 SLEE207 pKa = 4.24 MNKK210 pKa = 8.57 STSHH214 pKa = 5.87 YY215 pKa = 10.49 EE216 pKa = 4.07 SGLSTGLKK224 pKa = 10.51 LLGQTEE230 pKa = 4.48 LTNGNALTTAADD242 pKa = 3.59 ALRR245 pKa = 11.84 YY246 pKa = 9.01 SAAVGYY252 pKa = 10.15 RR253 pKa = 3.19
Molecular weight: 26.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.84
IPC2_protein 5.003
IPC_protein 4.863
Toseland 4.762
ProMoST 4.965
Dawson 4.813
Bjellqvist 4.952
Wikipedia 4.647
Rodwell 4.724
Grimsley 4.673
Solomon 4.8
Lehninger 4.762
Nozaki 4.914
DTASelect 5.016
Thurlkill 4.736
EMBOSS 4.673
Sillero 4.991
Patrickios 3.541
IPC_peptide 4.813
IPC2_peptide 4.991
IPC2.peptide.svr19 4.958
Protein with the highest isoelectric point:
>tr|A0A4P8W4G9|A0A4P8W4G9_9VIRU Nonstructural protein OS=Capybara microvirus Cap1_SP_175 OX=2585404 PE=4 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 RR3 pKa = 11.84 IVKK6 pKa = 9.74 KK7 pKa = 10.72 KK8 pKa = 10.12 KK9 pKa = 9.89 GATKK13 pKa = 9.5 MIEE16 pKa = 4.02 MPEE19 pKa = 3.98 FTNKK23 pKa = 8.24 YY24 pKa = 5.9 TAQQISDD31 pKa = 3.06 ITGISKK37 pKa = 8.72 RR38 pKa = 11.84 TIEE41 pKa = 3.61 RR42 pKa = 11.84 WRR44 pKa = 11.84 RR45 pKa = 11.84 SGIPGNMEE53 pKa = 3.49 GMKK56 pKa = 10.12 YY57 pKa = 10.16 QYY59 pKa = 11.41 LLTKK63 pKa = 10.78 LLLTGNKK70 pKa = 9.53 EE71 pKa = 3.94 IIIKK75 pKa = 10.81 GEE77 pKa = 3.74
Molecular weight: 8.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.168
IPC2_protein 9.619
IPC_protein 9.692
Toseland 10.555
ProMoST 10.058
Dawson 10.643
Bjellqvist 10.248
Wikipedia 10.76
Rodwell 11.257
Grimsley 10.687
Solomon 10.672
Lehninger 10.657
Nozaki 10.496
DTASelect 10.248
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.555
Patrickios 11.008
IPC_peptide 10.687
IPC2_peptide 8.668
IPC2.peptide.svr19 8.698
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1468
77
556
244.7
28.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.084 ± 1.373
0.954 ± 0.374
4.223 ± 0.526
8.243 ± 1.356
2.929 ± 0.753
6.471 ± 0.665
1.635 ± 0.367
7.084 ± 1.236
7.561 ± 2.241
5.45 ± 0.316
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.406 ± 0.483
5.722 ± 0.46
3.883 ± 0.821
4.223 ± 0.448
4.564 ± 0.485
7.425 ± 2.463
7.357 ± 1.125
3.474 ± 1.038
2.316 ± 0.28
5.995 ± 0.559
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here